{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T20:23:31Z","timestamp":1793132611878,"version":"4.5.0"},"reference-count":44,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T00:00:00Z","timestamp":1687392000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Austrian Federal Ministry of Transport, Innovation and Technology (BMVIT)","award":["COMET-Project 871132"],"award-info":[{"award-number":["COMET-Project 871132"]}]},{"name":"Austrian Federal Ministry of Transport, Innovation and Technology (BMVIT)","award":["ZK-17"],"award-info":[{"award-number":["ZK-17"]}]},{"name":"Austrian Science Fond (FWF)","award":["COMET-Project 871132"],"award-info":[{"award-number":["COMET-Project 871132"]}]},{"name":"Austrian Science Fond (FWF)","award":["ZK-17"],"award-info":[{"award-number":["ZK-17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Postnatal bone fractures of the growth plate (GP) are often associated with regenerative complications such as growth impairment. In order to understand the underlying processes of trauma-associated growth impairment within postnatal bone, an ex vivo rat femur slice model was developed. To achieve this, a 2 mm horizontal cut was made through the GP of rat femur prior to the organotypic culture being cultivated for 15 days in vitro. Histological analysis showed disrupted endochondral ossification, including disordered architecture, increased chondrocyte metabolic activity, and a loss of hypertrophic zone throughout the distal femur. Furthermore, altered expression patterns of Col2\u03b11, Acan, and ColX, and increased chondrocyte metabolic activity in the TZ and MZ at day 7 and day 15 postinjury were observed. STEM revealed the presence of stem cells, fibroblasts, and chondrocytes within the injury site at day 7. In summary, the findings of this study suggest that the ex vivo organotypic GP injury model could be a valuable tool for investigating the underlying mechanisms of GP regeneration post-trauma, as well as other tissue engineering and disease studies.<\/jats:p>","DOI":"10.3390\/cells12131687","type":"journal-article","created":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T01:54:56Z","timestamp":1687485296000},"page":"1687","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Disruption of Endochondral Ossification and Extracellular Matrix Maturation in an Ex Vivo Rat Femur Organotypic Slice Model Due to Growth Plate Injury"],"prefix":"10.3390","volume":"12","author":[{"given":"Vanessa","family":"Etschmaier","sequence":"first","affiliation":[{"name":"Research Unit for Experimental Neurotraumatology, Medical University of Graz, 8036 Graz, Austria"},{"name":"Department of Orthopaedics and Trauma, Medical University Graz, 8036 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2578-3833","authenticated-orcid":false,"given":"Muammer","family":"\u00dc\u00e7al","sequence":"additional","affiliation":[{"name":"Research Unit for Experimental Neurotraumatology, Medical University of Graz, 8036 Graz, Austria"},{"name":"Bio-Tech-Med Graz, 8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3156-7902","authenticated-orcid":false,"given":"Birgit","family":"Lohberger","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Trauma, Medical University Graz, 8036 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Absenger-Novak","sequence":"additional","affiliation":[{"name":"Center for Medical Research, Core Facility Imaging, Medical University of Graz, 8036 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dagmar","family":"Kolb","sequence":"additional","affiliation":[{"name":"Center for Medical Research, Core Facility Ultrastructure Analysis, Gottfried Schatz Research Center, Medical University of Graz, 8010 Graz, Austria"},{"name":"Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0385-4346","authenticated-orcid":false,"given":"Annelie","family":"Weinberg","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Trauma, Medical University Graz, 8036 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Sch\u00e4fer","sequence":"additional","affiliation":[{"name":"Research Unit for Experimental Neurotraumatology, Medical University of Graz, 8036 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.2106\/00004623-200304000-00021","article-title":"The biology of the growth plate","volume":"85","author":"Ballock","year":"2003","journal-title":"J. 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