{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T20:50:52Z","timestamp":1775681452953,"version":"3.50.1"},"reference-count":92,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T00:00:00Z","timestamp":1688428800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"National Science Centre (NCN) Poland","doi-asserted-by":"publisher","award":["2017\/27\/B\/NZ9\/00113"],"award-info":[{"award-number":["2017\/27\/B\/NZ9\/00113"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Telomerase activity has been found in the somatic tissues of rainbow trout. The enzyme is essential for maintaining telomere length but also assures homeostasis of the fish organs, playing an important role during tissue regeneration. The unique morphological and physiological characteristics of triploid rainbow trout, when compared to diploid specimens, make them a promising model for studies concerning telomerase activity. Thus, in this study, we examined the expression of the Tert gene in various organs of subadult and adult diploid and triploid rainbow trout females. Upregulated Tert mRNA transcription was observed in all the examined somatic tissues sampled from the triploid fish when compared to diploid individuals. Contrastingly, Tert expression in the ovaries was significantly decreased in the triploid specimens. Within the diploids, the highest expression of Tert was observed in the liver and in the ovaries of the subadult individuals. In the triploids, Tert expression was increased in the somatic tissues, while the ovaries exhibited lower activity of telomerase compared to other organs and decreased compared to the ovaries in the diploids. The ovaries of triploid individuals were underdeveloped, consisting of only a few oocytes. The lack of germ cells, which are usually characterized by high Tert expression, might be responsible for the decrease in telomerase activity in the triploid ovaries. The increase in Tert expression in triploid somatic tissues suggests that they require higher telomerase activity to cope with environmental stress and maintain internal homeostasis.<\/jats:p>","DOI":"10.3390\/cells12131772","type":"journal-article","created":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T01:42:47Z","timestamp":1688434967000},"page":"1772","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Telomerase Activity in Somatic Tissues and Ovaries of Diploid and Triploid Rainbow Trout (Oncorhynchus mykiss) Females"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0621-5902","authenticated-orcid":false,"given":"Ligia","family":"Panasiak","sequence":"first","affiliation":[{"name":"Department of Marine Biology and Biotechnology, Faculty of Oceanography and Geography, University of Gdansk, M. Pi\u0142sudskiego 46 Av., 81-378 Gdynia, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2977-7249","authenticated-orcid":false,"given":"Marcin","family":"Kuci\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Marine Biology and Biotechnology, Faculty of Oceanography and Geography, University of Gdansk, M. Pi\u0142sudskiego 46 Av., 81-378 Gdynia, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5205-608X","authenticated-orcid":false,"given":"Piotr","family":"Hliwa","sequence":"additional","affiliation":[{"name":"Department of Ichthyology and Aquaculture, University of Warmia and Mazury in Olsztyn, Warszawska St. 117, 10-719 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5256-3847","authenticated-orcid":false,"given":"Konrad","family":"Pomianowski","sequence":"additional","affiliation":[{"name":"Laboratory of Physiology of Marine Organisms, Genetics and Marine Biotechnology Department, Institute of Oceanology Polish Academy of Sciences, Powsta\u0144c\u00f3w Warszawy 55, 81-712 Sopot, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konrad","family":"Ocalewicz","sequence":"additional","affiliation":[{"name":"Department of Marine Biology and Biotechnology, Faculty of Oceanography and Geography, University of Gdansk, M. Pi\u0142sudskiego 46 Av., 81-378 Gdynia, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/350569a0","article-title":"Structure and Function of Telomeres","volume":"350","author":"Blackburn","year":"1991","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1002\/path.2090","article-title":"Hallmarks of Telomeres in Ageing Research","volume":"211","author":"Shay","year":"2007","journal-title":"J. Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1038\/554","article-title":"Expression of TERT in Early Premalignant Lesions and a Subset of Cells in Normal Tissues","volume":"19","author":"Kolquist","year":"1998","journal-title":"Nat. Genet."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1038\/sj.neo.7900134","article-title":"Immunohistochemical Detection of Telomerase (HTERT) Protein in Human Cancer Tissues and a Subset of Cells in Normal Tissues","volume":"3","author":"Hiyama","year":"2001","journal-title":"Neoplasia"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.exger.2007.03.007","article-title":"Telomerase and the Aging Process","volume":"42","author":"Hornsby","year":"2007","journal-title":"Exp. Gerontol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Anchelin, M., Murcia, L., Alcaraz-P\u00e9rez, F., Garc\u00eda-Navarro, E.M., and Cayuela, M.L. (2011). Behaviour of Telomere and Telomerase during Aging and Regeneration in Zebrafish. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0016955"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Lund, T.C., Glass, T.J., Tolar, J., and Blazar, B.R. (2009). Expression of Telomerase and Telomere Length Are Unaffected by Either Age or Limb Regeneration in Danio Rerio. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0007688"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Panasiak, L., Dobosz, S., and Ocalewicz, K. (2020). Telomere Dynamics in the Diploid and Triploid Rainbow Trout (Oncorhynchus mykiss) Assessed by Q-FISH Analysis. Genes, 11.","DOI":"10.3390\/genes11070786"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"62","DOI":"10.18632\/aging.100873","article-title":"Telomere Attrition and Restoration in the Normal Teleost Oryzias Latipes Are Linked to Growth Rate and Telomerase Activity at Each Life Stage","volume":"8","author":"Hatakeyama","year":"2016","journal-title":"Aging"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/S0014-5793(98)01020-5","article-title":"Telomerase Activity in \u201cImmortal\u201d Fish 1","volume":"434","author":"Klapper","year":"1998","journal-title":"FEBS Lett."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"958","DOI":"10.3181\/0712-RM-345","article-title":"Upregulation of Telomerase Function during Tissue Regeneration","volume":"233","author":"Elmore","year":"2008","journal-title":"Exp. Biol. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"20160449","DOI":"10.1098\/rstb.2016.0449","article-title":"Ectothermic Telomeres: It\u2019s Time They Came in from the Cold","volume":"373","author":"Olsson","year":"2018","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1440-169X.2008.00986.x","article-title":"Tert Expression and Telomerase Activity in Gonads and Somatic Cells of the Japanese Medaka (Oryzias latipes)","volume":"50","author":"Pfennig","year":"2008","journal-title":"Dev. Growth Differ."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.cbpc.2015.09.004","article-title":"Modulation of Telomerase Activity in Fish Muscle by Biological and Environmental Factors","volume":"178","author":"Peterson","year":"2015","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s12031-007-0072-x","article-title":"Molecular Cloning and Characterization of the Zebrafish (Danio rerio) Telomerase Catalytic Subunit (Telomerase Reverse Transcriptase, TERT)","volume":"34","author":"Lau","year":"2007","journal-title":"J. Mol. Neurosci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1089\/zeb.2005.1.349","article-title":"Vertebrate Marine Species as Model Systems for Studying Telomeres and Telomerase","volume":"1","author":"McChesney","year":"2005","journal-title":"Zebrafish"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/S0531-5565(03)00108-6","article-title":"The Zebrafish as a Vertebrate Model of Functional Aging and Very Gradual Senescence","volume":"38","author":"Kishi","year":"2003","journal-title":"Exp. Gerontol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/j.cell.2015.01.038","article-title":"A Platform for Rapid Exploration of Aging and Diseases in a Naturally Short-Lived Vertebrate","volume":"160","author":"Harel","year":"2015","journal-title":"Cell"},{"key":"ref_19","unstructured":"The State of World Fisheries 2022 (2023, May 05). FAO|Food and Agriculture Organization of the United Nations. Available online: https:\/\/www.fao.org\/publications\/sofia\/2022\/en\/."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/S1096-4959(02)00167-7","article-title":"Status and Opportunities for Genomics Research with Rainbow Trout","volume":"133","author":"Thorgaard","year":"2002","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Bio."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1016\/j.theriogenology.2011.05.028","article-title":"Altered Gene Transcription and Telomere Length in Trout Embryo and Larvae Obtained with DNA Cryodamaged Sperm","volume":"76","year":"2011","journal-title":"Theriogenology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1159\/000130217","article-title":"Triploidy in Rainbow Trout","volume":"11","author":"Cuellar","year":"1972","journal-title":"Cytogenet. Genome Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1093\/genetics\/93.4.961","article-title":"Adult Triploids in a Rainbow Trout Family","volume":"93","author":"Thorgaard","year":"1979","journal-title":"Genetics"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/0044-8486(82)90144-2","article-title":"Triploid Rainbow Trout Identified by Flow Cytometry","volume":"29","author":"Thorgaard","year":"1982","journal-title":"Aquaculture"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.aquaculture.2003.08.019","article-title":"Effects of Post-Ovulatory Oocyte Ageing and Temperature on Egg Quality and on the Occurrence of Triploid Fry in Rainbow Trout","volume":"231","author":"Aegerter","year":"2004","journal-title":"Oncorhynchus Mykiss. Aquac."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1139\/G09-009","article-title":"Karyotype Variation in the Albino Rainbow Trout (Oncorhynchus mykiss (Walbaum))","volume":"52","author":"Ocalewicz","year":"2009","journal-title":"Genome"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"161","DOI":"10.2183\/pjab.62.161","article-title":"Triploid Rainbow Trouts Induced by Polyethylene Glycol","volume":"62","author":"Ueda","year":"1986","journal-title":"Proc. Jpn. Acad. B"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/BF00266992","article-title":"Production of Second Generation Triploid and Tetraploid Rainbow Trout by Mating Tetraploid Males and Diploid Females ? Potential of Tetraploid Fish","volume":"72","author":"Chourrout","year":"1986","journal-title":"Theor. Appl. Genet."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0044-8486(87)90233-X","article-title":"Evaluation of Juvenile Rainbow Trout Survival and Growth in Half-Sib Families from Diploid and Tetraploid Sires","volume":"65","author":"Blanc","year":"1987","journal-title":"Aquaculture"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0044-8486(91)90142-T","article-title":"Early Growth and Survival of Heat-Shocked and Tetraploid-Derived Triploid Rainbow Trout (Oncorhynchus mykiss)","volume":"96","author":"Myers","year":"1991","journal-title":"Aquaculture"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0044-8486(86)90180-8","article-title":"Ploidy Manipulation and Performance","volume":"57","author":"Thorgaard","year":"1986","journal-title":"Aquaculture"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1023\/A:1003332526659","article-title":"Ploidy Induction and Sex Control in Fish","volume":"384","author":"Pandian","year":"1998","journal-title":"Hydrobiologia"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1159\/000353301","article-title":"Genomic Constitution and Atypical Reproduction in Polyploid and Unisexual Lineages of the Misgurnus Loach, a Teleost Fish","volume":"140","author":"Arai","year":"2013","journal-title":"Cytogenet. Genome Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1080\/10641269991319162","article-title":"The Physiology and Behavior of Triploid Fishes","volume":"7","author":"Benfey","year":"1999","journal-title":"Rev. Fish. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.aquaculture.2009.04.036","article-title":"Polyploid Fish and Shellfish: Production, Biology and Applications to Aquaculture for Performance Improvement and Genetic Containment","volume":"293","author":"Piferrer","year":"2009","journal-title":"Aquaculture"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1023\/A:1013776931796","article-title":"Genetic Manipulations in Aquaculture: A Review of Stock Improvement by Classical and Modern Technologies","volume":"111","author":"Hulata","year":"2001","journal-title":"Genetica"},{"key":"ref_37","first-page":"266","article-title":"A Review of Ploidy Manipulations in Aquaculture: The Israeli Experience","volume":"58","author":"Rothbard","year":"2006","journal-title":"Isr. J. Aquac. Bamidgeh."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1080\/02755947.2011.561163","article-title":"Performance of Diploid and Triploid Rainbow Trout Stocked in Idaho Alpine Lakes","volume":"31","author":"Koenig","year":"2011","journal-title":"N. Am. J. Fish. Manag."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1007\/PL00011808","article-title":"Growth Performance and Gonadal Development of Growth Enhanced Transgenic Tilapia Oreochromis niloticus (L.) Following Heat-Shock-Induced Triploidy","volume":"1","author":"Razak","year":"1999","journal-title":"Mar. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.aquaculture.2003.09.046","article-title":"Triploid Hybridization of Fast-Growing Transgenic Mud Loach Misgurnus Mizolepis Male to Cyprinid Loach Misgurnus anguillicaudatus Female: The First Performance Study on Growth and Reproduction of Transgenic Polyploid Hybrid Fish","volume":"231","author":"Nam","year":"2004","journal-title":"Aquaculture"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1080\/02755947.2011.608616","article-title":"Triploid Grass Carp: Triploid Induction, Sterility, Reversion, and Certification","volume":"31","author":"Zajicek","year":"2011","journal-title":"N. Am. J. Fish. Manag."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"106929","DOI":"10.1016\/j.anireprosci.2022.106929","article-title":"Application of High Hydrostatic Pressure (HHP) Shock to Induce Triploid Development in the European Grayling (Thymallus thymallus L.)","volume":"237","author":"Hliwa","year":"2022","journal-title":"Anim. Reprod. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.aquaculture.2013.01.015","article-title":"The Effect of Triploidization of Atlantic Cod (Gadus morhua L.) on Survival, Growth and Deformities during Early Life Stages","volume":"388\u2013391","author":"Opstad","year":"2013","journal-title":"Aquaculture"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.aquaculture.2019.03.037","article-title":"Surrogate Production of Salmo Salar Oocytes and Sperm in Triploid Oncorhynchus Mykiss by Germ Cell Transplantation Technology","volume":"506","author":"Hattori","year":"2019","journal-title":"Aquaculture"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"100594","DOI":"10.1016\/j.animal.2022.100594","article-title":"Ovarian Transcriptome Analysis of Diploid and Triploid Rainbow Trout Revealed New Pathways Related to Gonadal Development and Fertility","volume":"16","author":"Nynca","year":"2022","journal-title":"Animal"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1086\/700218","article-title":"Ploidy Has Minimal Effect on Hypoxia Tolerance at High Temperature in Rainbow Trout (Oncorhynchus mykiss)","volume":"91","author":"Benfey","year":"2018","journal-title":"Physiol. Biochem. Zool."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"921709","DOI":"10.3389\/fphys.2022.921709","article-title":"Effects of Acute Hypoxic Stress on Physiological and Hepatic Metabolic Responses of Triploid Rainbow Trout (Oncorhynchus mykiss)","volume":"13","author":"Han","year":"2022","journal-title":"Front. Physiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.mce.2013.09.024","article-title":"Ploidy Effects on Genes Regulating Growth Mechanisms during Fasting and Refeeding in Juvenile Rainbow Trout (Oncorhynchus mykiss)","volume":"382","author":"Cleveland","year":"2014","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"110240","DOI":"10.1016\/j.vetimm.2021.110240","article-title":"Effect of Cortisol on the Immune-like Response of Rainbow Trout (Oncorhynchus mykiss) Myotubes Challenged with Piscirickettsia Salmonis","volume":"237","author":"Carrizo","year":"2021","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_51","unstructured":"Zawistowski, S. (1983). Histological Technique, Histology and the Principles of Histopathology, PZWL."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1080\/19425120.2011.555724","article-title":"A Standardized Terminology for Describing Reproductive Development in Fishes","volume":"3","author":"Wyanski","year":"2011","journal-title":"Mar. Coast. Fish."},{"key":"ref_53","first-page":"13","article-title":"Cytogenetic Markers for X Chromosome in a Karyotype of Rainbow Trout from Rutki Strain (Poland)","volume":"50","author":"Ocalewicz","year":"2002","journal-title":"Folia Biol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S1464793100005613","article-title":"Chromosome Evolution in the Salmonidae (Pisces): An Update","volume":"76","author":"Phillips","year":"2001","journal-title":"Biol. Rev."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1467-2979.2007.00269.x","article-title":"The Physiology of Triploid Fish: Current Knowledge and Comparisons with Diploid Fish","volume":"9","author":"Maxime","year":"2008","journal-title":"Fish Fish."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0074-7696(08)61648-4","article-title":"Cell Size and Nuclear DNA Content in Vertebrates","volume":"44","author":"Szarski","year":"1976","journal-title":"Int. Rev. Cytol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.aquaculture.2013.09.034","article-title":"The Effect of Triploidy on the Culture Performance, Deformity Prevalence, and Heart Morphology in Atlantic Salmon","volume":"416\u2013417","author":"Fraser","year":"2013","journal-title":"Aquaculture"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Vera, L.M., Metochis, C., Taylor, J.F., Clarkson, M., Skj\u00e6rven, K.H., Migaud, H., and Tocher, D.R. (2017). Early Nutritional Programming Affects Liver Transcriptome in Diploid and Triploid Atlantic Salmon, Salmo Salar. BMC Genom., 18.","DOI":"10.1186\/s12864-017-4264-7"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"14080","DOI":"10.1073\/pnas.2334605100","article-title":"Cell Size as a Link between Noncoding DNA and Metabolic Rate Scaling","volume":"100","author":"Kozlowski","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Christensen, K.A., Sakhrani, D., Rondeau, E.B., Richards, J., Koop, B.F., and Devlin, R.H. (2019). Effect of Triploidy on Liver Gene Expression in Coho Salmon (Oncorhynchus kisutch) under Different Metabolic States. BMC Genom., 20.","DOI":"10.1186\/s12864-019-5655-8"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.ygcen.2012.06.006","article-title":"Elevated Expressions of GH\/IGF Axis Genes in Triploid Crucian Carp","volume":"178","author":"Zhong","year":"2012","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Harvey, A., Fjelldal, P.G., Solberg, M.F., Hansen, T.W., and Glover, K.A. (2017). Ploidy Elicits a Whole-Genome Dosage Effect: Growth of Triploid Atlantic Salmon Is Linked to the Genetic Origin of the Second Maternal Chromosome Set. BMC Genet., 18.","DOI":"10.1186\/s12863-017-0502-x"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1007\/s10126-017-9730-3","article-title":"De Novo Transcriptome Characterization and Growth-Related Gene Expression Profiling of Diploid and Triploid Bighead Catfish (Clarias macrocephalus G\u00fcnther, 1864)","volume":"19","author":"Chatchaiphan","year":"2017","journal-title":"Mar. Biotechnol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.aquaculture.2011.05.010","article-title":"Effect of Triploidy on Growth and Ionoregulatory Performance in Ocean-Type Chinook Salmon: A Quantitative Genetics Approach","volume":"362\u2013363","author":"Shrimpton","year":"2012","journal-title":"Aquaculture"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"736149","DOI":"10.1016\/j.aquaculture.2020.736149","article-title":"Oxidative Stress-Related Gene Expression in Diploid and Triploid Rainbow Trout (Oncorhynchus mykiss) Fed Diets with Organic and Inorganic Zinc","volume":"533","author":"Meiler","year":"2021","journal-title":"Aquaculture"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1038\/hdy.2009.108","article-title":"Transcriptional Differences between Triploid and Diploid Chinook Salmon (Oncorhynchus tshawytscha) during Live Vibrio Anguillarum Challenge","volume":"104","author":"Ching","year":"2009","journal-title":"Heredity"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Henriques, C.M., Carneiro, M.C., Tenente, I.M., Jacinto, A., and Ferreira, M.G. (2013). Telomerase Is Required for Zebrafish Lifespan. PLoS Genet., 9.","DOI":"10.1371\/journal.pgen.1003214"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Yu, R.M., Chen, E.X., Kong, R.Y., Ng, P.K., Mok, H.O., and Au, D.W. (2006). Hypoxia Induces Telomerase Reverse Transcriptase (TERT) Gene Expression in Non-Tumor Fish Tissues in Vivo: The Marine Medaka (Oryzias melastigma) Model. BMC Mol. Biol., 7.","DOI":"10.1186\/1471-2199-7-27"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.cbpc.2008.08.005","article-title":"Japanese Medaka: A New Vertebrate Model for Studying Telomere and Telomerase Biology","volume":"149","author":"Au","year":"2009","journal-title":"Comp. Biochem. Physiol. Part\u2014C Toxicol. Pharmacol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.gene.2014.03.006","article-title":"Molecular Cloning and Measurement of Telomerase Reverse Transcriptase (TERT) Transcription Patterns in Tissues of European Hake (Merluccius merluccius) and Atlantic Cod (Gadus morhua) during Aging","volume":"541","author":"Bilbao","year":"2014","journal-title":"Gene"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1089\/zeb.2022.0012","article-title":"Telomerase Activity in Androgenetic Rainbow Trout with Growth Deficiency and in Normally Developed Individuals","volume":"19","author":"Panasiak","year":"2022","journal-title":"Zebrafish"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2857","DOI":"10.1519\/JSC.0b013e3181e840f3","article-title":"The Mechanisms of Muscle Hypertrophy and Their Application to Resistance Training","volume":"24","author":"Schoenfeld","year":"2010","journal-title":"J. Strength. Cond. Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1002\/1097-010X(20001201)287:7<493::AID-JEZ5>3.0.CO;2-8","article-title":"Effect of Induced Triploidy on Fin Regeneration of Juvenile Rainbow Trout, Oncorhynchus mykiss","volume":"287","author":"Alonso","year":"2000","journal-title":"J. Exp. Zoo."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1134\/S002689332003005X","article-title":"Healthy Aging: Antioxidants, Uncouplers And\/or Telomerase?","volume":"54","author":"Yegorov","year":"2020","journal-title":"Mol. Biol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.ygeno.2006.08.014","article-title":"Genome Multiplication as Adaptation to Tissue Survival: Evidence from Gene Expression in Mammalian Heart and Liver","volume":"89","author":"Anatskaya","year":"2007","journal-title":"Genomics"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1046\/j.1440-1746.2000.02207.x","article-title":"Reactive Oxygen and Mechanisms of Inflammatory Liver Injury","volume":"15","author":"Jaeschke","year":"2000","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1139\/f04-110","article-title":"Oxygen and Carbon Dioxide Transport during Sustained Exercise in Diploid and Triploid Chinook Salmon (Oncorhynchus tshawytscha)","volume":"61","author":"Bernier","year":"2004","journal-title":"Can. J. Fish. Aquat. Sci."},{"key":"ref_78","first-page":"361","article-title":"Physiological Response of Triploid Rainbow Trout to Exhaustive Exercise","volume":"57","author":"Kobayashi","year":"2009","journal-title":"Aquacult. Sci."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1002\/(SICI)1520-6408(1999)25:4<397::AID-DVG13>3.0.CO;2-J","article-title":"Telomerase Activity and Telomere Detection during Early Bovine Development","volume":"25","author":"Betts","year":"1999","journal-title":"Dev. Genet."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1530\/REP-10-0008","article-title":"Telomerase in the Ovary","volume":"140","author":"Liu","year":"2010","journal-title":"Reproduction"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1620\/tjem.190.231","article-title":"Telomerase Activity in Normal Ovaries and Premature Ovarian Failure","volume":"190","author":"Kinugawa","year":"2000","journal-title":"Tohoku J. Exp. Med."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0303-7207(00)00430-5","article-title":"Dynamics of the Membrana Granulosa during Expansion of the Ovarian Follicular Antrum","volume":"171","author":"Rodgers","year":"2001","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.anireprosci.2007.02.003","article-title":"Telomerase Activity in Pig Granulosa Cells Proliferating and Differentiating in Vitro","volume":"104","author":"Chronowska","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.mad.2009.01.003","article-title":"Telomeres Shorten While Tert Expression Increases during Ageing of the Short-Lived Fish Nothobranchius Furzeri","volume":"130","author":"Hartmann","year":"2009","journal-title":"Mech. Ageing Dev."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.cbpc.2011.05.005","article-title":"Characterization of Telomeres and Telomerase Expression in Xiphophorus","volume":"155","author":"Downs","year":"2012","journal-title":"Comp. Biochem. Physiol. Part C Toxicol. Pharmacol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1530\/jrf.0.1130197","article-title":"Long-Term, Quantitative Analysis of Gametogenesis in Autotriploid Rainbow Trout, Oncorhynchus Mykiss","volume":"113","author":"Carrasco","year":"1998","journal-title":"Reproduction"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1111\/j.1095-8649.1984.tb04886.x","article-title":"Sexual Maturation in Triploid Rainbow Trout, Salmo Gairdneri Richardson","volume":"25","author":"Lincoln","year":"1984","journal-title":"J. Fish Biol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1002\/(SICI)1097-010X(19990901)284:4<466::AID-JEZ13>3.0.CO;2-G","article-title":"Gonadal Sex Reversal in Triploid Rainbow Trout (Oncorhynchus mykiss)","volume":"284","author":"Krisfalusi","year":"1999","journal-title":"J. Exp. Zool."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1111\/j.1095-8649.2010.02613.x","article-title":"Comparison of Reproductive Development in Triploid and Diploid Female Rainbow Trout, Oncorhynchus Mykiss","volume":"76","author":"Han","year":"2010","journal-title":"J. Fish Biol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.tice.2011.11.002","article-title":"Apoptosis, Cell Proliferation and Vitellogenesis during the Folliculogenesis and Follicular Growth in Teleost Fish","volume":"44","author":"Domingos","year":"2012","journal-title":"Tissue Cell"},{"key":"ref_91","first-page":"5917","article-title":"Estrogen Activates Telomerase","volume":"59","author":"Kyo","year":"1999","journal-title":"Cancer Res."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"20160446","DOI":"10.1098\/rstb.2016.0446","article-title":"Somatic Growth and Telomere Dynamics in Vertebrates: Relationships, Mechanisms and Consequences","volume":"373","author":"Monaghan","year":"2018","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/13\/1772\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:05:39Z","timestamp":1760126739000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/13\/1772"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,4]]},"references-count":92,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["cells12131772"],"URL":"https:\/\/doi.org\/10.3390\/cells12131772","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,4]]}}}