{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T03:47:37Z","timestamp":1784000857287,"version":"3.55.0"},"reference-count":79,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T00:00:00Z","timestamp":1690416000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["EY026614"],"award-info":[{"award-number":["EY026614"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["P30EY031631"],"award-info":[{"award-number":["P30EY031631"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute P30 Core","doi-asserted-by":"publisher","award":["EY026614"],"award-info":[{"award-number":["EY026614"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute P30 Core","doi-asserted-by":"publisher","award":["P30EY031631"],"award-info":[{"award-number":["P30EY031631"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Retinal ganglion cells (RGCs) are the sole output neurons conveying visual stimuli from the retina to the brain, and dysfunction or loss of RGCs is the primary determinant of visual loss in traumatic and degenerative ocular conditions. Currently, there is a lack of RGC-specific Cre mouse lines that serve as invaluable tools for manipulating genes in RGCs and studying the genetic basis of RGC diseases. The RNA-binding protein with multiple splicing (RBPMS) is identified as the specific marker of all RGCs. Here, we report the generation and characterization of a knock-in mouse line in which a P2A-CreERT2 coding sequence is fused in-frame to the C-terminus of endogenous RBPMS, allowing for the co-expression of RBPMS and CreERT2. The inducible Rbpms-CreERT2 mice exhibited a high recombination efficiency in activating the expression of the tdTomato reporter gene in nearly all adult RGCs as well as in differentiated RGCs starting at E13.5. Additionally, both heterozygous and homozygous Rbpms-CreERT2 knock-in mice showed no detectable defect in the retinal structure, visual function, and transcriptome. Together, these results demonstrated that the Rbpms-CreERT2 knock-in mouse can serve as a powerful and highly desired genetic tool for lineage tracing, genetic manipulation, retinal physiology study, and ocular disease modeling in RGCs.<\/jats:p>","DOI":"10.3390\/cells12151951","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T01:56:16Z","timestamp":1690509376000},"page":"1951","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Inducible Rbpms-CreERT2 Mouse Line for Studying Gene Function in Retinal Ganglion Cell Physiology and Disease"],"prefix":"10.3390","volume":"12","author":[{"given":"Luming","family":"Guo","sequence":"first","affiliation":[{"name":"College of Life Sciences, Zhejiang University, Hangzhou 310058, China"},{"name":"Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2891-670X","authenticated-orcid":false,"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"},{"name":"James and Jean Culver Vision Discovery Institute, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"},{"name":"James and Jean Culver Vision Discovery Institute, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuchun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang University, Hangzhou 310058, China"},{"name":"Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8611-4943","authenticated-orcid":false,"given":"Ebenezer","family":"Quainoo","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"},{"name":"James and Jean Culver Vision Discovery Institute, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rithwik","family":"Koppaka","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaping","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia B.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"},{"name":"James and Jean Culver Vision Discovery Institute, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5278-4554","authenticated-orcid":false,"given":"Lin","family":"Gan","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"},{"name":"James and Jean Culver Vision Discovery Institute, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8936","DOI":"10.1523\/JNEUROSCI.18-21-08936.1998","article-title":"The major cell populations of the mouse retina","volume":"18","author":"Jeon","year":"1998","journal-title":"J. Neurosci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.neuron.2012.10.002","article-title":"The Neuronal Organization of the Retina","volume":"76","author":"Masland","year":"2012","journal-title":"Neuron"},{"key":"ref_3","first-page":"285","article-title":"Ganglion cell distribution in the retina of the mouse","volume":"20","author":"Olsen","year":"1981","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4886","DOI":"10.1167\/iovs.10-5921","article-title":"Morphology and Immunoreactivity of Retrogradely Double-Labeled Ganglion Cells in the Mouse Retina","volume":"52","author":"Pang","year":"2011","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/cne.20631","article-title":"Diversity of ganglion cells in the mouse retina: Unsupervised morphological classification and its limits","volume":"489","author":"Kong","year":"2005","journal-title":"J. Comp. Neurol."},{"key":"ref_6","first-page":"E2391","article-title":"The most numerous ganglion cell type of the mouse retina is a selective feature detector","volume":"109","author":"Zhang","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1146\/annurev-neuro-071714-034120","article-title":"The Types of Retinal Ganglion Cells: Current Status and Implications for Neuronal Classification","volume":"38","author":"Sanes","year":"2015","journal-title":"Annu. Rev. Neurosci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1038\/s41467-018-05134-3","article-title":"Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes","volume":"9","author":"Rheaume","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/j.neuron.2019.11.006","article-title":"Single-Cell Profiles of Retinal Ganglion Cells Differing in Resilience to Injury Reveal Neuroprotective Genes","volume":"104","author":"Tran","year":"2019","journal-title":"Neuron"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.devcel.2020.04.009","article-title":"Single-Cell Analysis of Human Retina Identifies Evolutionarily Conserved and Species-Specific Mechanisms Controlling Development","volume":"53","author":"Lu","year":"2020","journal-title":"Dev. Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"111040","DOI":"10.1016\/j.celrep.2022.111040","article-title":"Unified classification of mouse retinal ganglion cells using function, morphology, and gene expression","volume":"40","author":"Goetz","year":"2022","journal-title":"Cell Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.neuroscience.2022.07.013","article-title":"Vision-Dependent and -Independent Molecular Maturation of Mouse Retinal Ganglion Cells","volume":"508","author":"Whitney","year":"2023","journal-title":"Neuroscience"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1038\/sj.eye.6701591","article-title":"Hereditary optic neuropathies","volume":"18","author":"Newman","year":"2004","journal-title":"Eye"},{"key":"ref_14","first-page":"145","article-title":"Inherited mitochondrial optic neuropathies","volume":"46","author":"Griffiths","year":"2009","journal-title":"J. Med. Genet."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.preteyeres.2010.11.002","article-title":"Mitochondrial optic neuropathies\u2013Disease mechanisms and therapeutic strategies","volume":"30","author":"Griffiths","year":"2011","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"108571","DOI":"10.1016\/j.exer.2021.108571","article-title":"Commonalities of optic nerve injury and glaucoma-induced neurodegeneration: Insights from transcriptome-wide studies","volume":"207","author":"Wang","year":"2021","journal-title":"Exp. Eye Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1038\/79944","article-title":"OPA1, encoding a dynamin-related GTPase, is mutated in autosomal dominant optic atrophy linked to chromosome 3q28","volume":"26","author":"Alexander","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1093\/hmg\/10.13.1359","article-title":"OPA1 mutations in patients with autosomal dominant optic atrophy and evidence for semi-dominant inheritance","volume":"10","author":"Pesch","year":"2001","journal-title":"Hum. Mol. Genet."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1001\/archopht.125.1.30","article-title":"Genetic Etiologies of Glaucoma","volume":"125","author":"Wiggs","year":"2007","journal-title":"Arch. Ophthalmol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e144","DOI":"10.1016\/S2214-109X(20)30489-7","article-title":"Causes of blindness and vision impairment in 2020 and trends over 30 years, and prevalence of avoidable blindness in relation to VISION 2020: The Right to Sight: An analysis for the Global Burden of Disease Study","volume":"9","author":"Steinmetz","year":"2021","journal-title":"Lancet Glob. Health"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"E1559","DOI":"10.1073\/pnas.1421535112","article-title":"Two transcription factors, Pou4f2 and Isl1, are sufficient to specify the retinal ganglion cell fate","volume":"112","author":"Wu","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/381603a0","article-title":"Role of transcription factors a Brn-3.1 and Brn-3.2 in auditory and visual system development","volume":"381","author":"Erkman","year":"1996","journal-title":"Nature"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3920","DOI":"10.1073\/pnas.93.9.3920","article-title":"POU domain factor Brn3b is required for the development of a large set of retinal ganglion cells","volume":"93","author":"Gan","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/s13064-021-00155-z","article-title":"Genetic interplay between transcription factor Pou4f1\/Brn3a and neurotrophin receptor Ret in retinal ganglion cell type specification","volume":"16","author":"Muzyka","year":"2021","journal-title":"Neural Dev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"E3974","DOI":"10.1073\/pnas.1618551114","article-title":"Molecular codes for cell type specification in Brn3 retinal ganglion cells","volume":"114","author":"Sajgo","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"18429","DOI":"10.1074\/jbc.M113.478503","article-title":"Transcription Factors SOX4 and SOX11 Function Redundantly to Regulate the Development of Mouse Retinal Ganglion Cells","volume":"288","author":"Jiang","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1016\/j.neuron.2017.01.029","article-title":"SoxC Transcription Factors Promote Contralateral Retinal Ganglion Cell Differentiation and Axon Guidance in the Mouse Visual System","volume":"93","author":"Kuwajima","year":"2017","journal-title":"Neuron"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1038\/s41467-021-21704-4","article-title":"Single cell transcriptomics reveals lineage trajectory of retinal ganglion cells in wild-type and Atoh7-null retinas","volume":"12","author":"Wu","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.1242\/dev.128.13.2497","article-title":"Math5 is required for retinal ganglion cell and optic nerve formation","volume":"128","author":"Brown","year":"2001","journal-title":"Development"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"21690","DOI":"10.1073\/pnas.2006888117","article-title":"The Atoh7 remote enhancer provides transcriptional robustness during retinal ganglion cell development","volume":"117","author":"Miesfeld","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1097\/00001756-200004270-00008","article-title":"Functional human heme oxygenase has a neuroprotective effect on adult rat ganglion cells after pressure-induced ischemia","volume":"11","author":"Hegazy","year":"2000","journal-title":"NeuroReport"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1038\/mt.2015.198","article-title":"Virus-mediated EpoR76E Therapy Slows Optic Nerve Axonopathy in Experimental Glaucoma","volume":"24","author":"Bond","year":"2016","journal-title":"Mol. Ther."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3943","DOI":"10.1167\/iovs.18-23784","article-title":"Ecel1 Knockdown With an AAV2-Mediated CRISPR\/Cas9 System Promotes Optic Nerve Damage-Induced RGC Death in the Mouse Retina","volume":"59","author":"Sato","year":"2018","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1146\/annurev-neuro-062012-170307","article-title":"Genetic Approaches to Neural Circuits in the Mouse","volume":"36","author":"Zeng","year":"2013","journal-title":"Annu. Rev. Neurosci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"76","DOI":"10.3389\/fncir.2014.00076","article-title":"Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation","volume":"8","author":"Harris","year":"2014","journal-title":"Front. Neural Circuits"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2058","DOI":"10.1016\/j.celrep.2017.01.075","article-title":"Diverse Central Projection Patterns of Retinal Ganglion Cells","volume":"18","author":"Martersteck","year":"2017","journal-title":"Cell Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1002\/cne.25065","article-title":"Identification of retinal ganglion cell types and brain nuclei expressing the transcription factor Brn3c\/Pou4f3 using a Cre recombinase knock-in allele","volume":"529","author":"Parmhans","year":"2021","journal-title":"J. Comp. Neurol."},{"key":"ref_38","first-page":"705","article-title":"Pou4f2 knock-in Cre mouse: A multifaceted genetic tool for vision researchers","volume":"22","author":"Simmons","year":"2016","journal-title":"Mol. Vis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1016\/0306-4522(84)90195-7","article-title":"Thy-1 antigen: A ganglion cell specific marker in rodent retina","volume":"11","author":"Barnstable","year":"1984","journal-title":"Neuroscience"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.brainres.2008.03.038","article-title":"Comparison of the ontogeny of the vesicular glutamate transporter 3 (VGLUT3) with VGLUT1 and VGLUT2 in the rat retina","volume":"1215","author":"Stella","year":"2008","journal-title":"Brain Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1016\/j.neuron.2015.02.017","article-title":"Subtype-specific regeneration of retinal ganglion cells following axotomy: Effects of osteopontin and mTOR signaling","volume":"85","author":"Duan","year":"2015","journal-title":"Neuron"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.neuroscience.2009.10.021","article-title":"Characterization of transgenic mouse lines expressing Cre recombinase in the retina","volume":"165","author":"Ivanova","year":"2010","journal-title":"Neuroscience"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0092-8674(01)00295-1","article-title":"Pax6 Is Required for the Multipotent State of Retinal Progenitor Cells","volume":"105","author":"Marquardt","year":"2001","journal-title":"Cell"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e23305","DOI":"10.1002\/dvg.23305","article-title":"A Chrnb3-Cre BAC transgenic mouse line for manipulation of gene expression in retinal ganglion cells","volume":"57","author":"Drayson","year":"2019","journal-title":"Genesis"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.1007\/s00246-019-02180-6","article-title":"Exploring the Activities of RBPMS Proteins in Myocardial Biology","volume":"40","author":"Akerberg","year":"2019","journal-title":"Pediatr. Cardiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1006\/dbio.2002.0678","article-title":"A role for the RNA-binding protein, hermes, in the regulation of heart development","volume":"247","author":"Gerber","year":"2002","journal-title":"Dev. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/j.devcel.2022.03.017","article-title":"RBPMS is an RNA-binding protein that mediates cardiomyocyte binucleation and cardiovascular development","volume":"57","author":"Gan","year":"2022","journal-title":"Dev. Cell"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"11895","DOI":"10.1093\/nar\/gkac1048","article-title":"Phosphorylation of the smooth muscle master splicing regulator RBPMS regulates its splicing activity","volume":"50","author":"Barnhart","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1167\/iovs.09-4098","article-title":"RNA Binding Protein with Multiple Splicing: A New Marker for Retinal Ganglion Cells","volume":"51","author":"Kwong","year":"2010","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1002\/cne.23521","article-title":"The RNA binding protein RBPMS is a selective marker of ganglion cells in the mammalian retina","volume":"522","author":"Rodriguez","year":"2014","journal-title":"J. Comp. Neurol."},{"key":"ref_51","first-page":"226","article-title":"Pan-retinal ganglion cell markers in mice, rats, and rhesus macaques","volume":"44","author":"Li","year":"2023","journal-title":"Zool. Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"9694","DOI":"10.1167\/iovs.11-7869","article-title":"Quantitative analysis of retinal ganglion cell survival with Rbpms immunolabeling in animal models of optic neuropathies","volume":"52","author":"Kwong","year":"2011","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1016\/j.ymthe.2022.01.035","article-title":"NMNAT2 is downregulated in glaucomatous RGCs, and RGC-specific gene therapy rescues neurodegeneration and visual function","volume":"30","author":"Fang","year":"2022","journal-title":"Mol. Ther."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1016\/j.neuron.2022.06.002","article-title":"Overlapping transcriptional programs promote survival and axonal regeneration of injured retinal ganglion cells","volume":"110","author":"Jacobi","year":"2022","journal-title":"Neuron"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"148377","DOI":"10.1016\/j.brainres.2023.148377","article-title":"Transcriptomic profiling of retinal cells reveals a subpopulation of microglia\/macrophages expressing Rbpms marker of retinal ganglion cells (RGCs) that confound identification of RGCs","volume":"1811","author":"Theune","year":"2023","journal-title":"Brain Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2607","DOI":"10.1016\/j.neuron.2022.06.003","article-title":"Core transcription programs controlling injury-induced neurodegeneration of retinal ganglion cells","volume":"110","author":"Tian","year":"2022","journal-title":"Neuron"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/nn.2467","article-title":"A robust and high-throughput Cre reporting and characterization system for the whole mouse brain","volume":"13","author":"Madisen","year":"2010","journal-title":"Nat. Neurosci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/dvdy.24067","article-title":"Bhlhb5 is required for the subtype development of retinal amacrine and bipolar cells in mice","volume":"243","author":"Huang","year":"2014","journal-title":"Dev. Dyn."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"108894","DOI":"10.1016\/j.exer.2021.108894","article-title":"Effect of long-term chronic hyperhomocysteinemia on retinal structure and function in the cystathionine-\u03b2-synthase mutant mouse","volume":"214","author":"Xiao","year":"2022","journal-title":"Exp. Eye Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1080\/19420862.2015.1008351","article-title":"Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells","volume":"7","author":"Chng","year":"2015","journal-title":"MAbs"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4324","DOI":"10.1093\/nar\/27.22.4324","article-title":"Temporally-controlled site-specific mutagenesis in the basal layer of the epidermis: Comparison of the recombinase activity of the tamoxifen-inducible Cre-ER(T) and Cre-ER(T2) recombinases","volume":"27","author":"Indra","year":"1999","journal-title":"Nucleic Acids Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"4762","DOI":"10.1523\/JNEUROSCI.15-07-04762.1995","article-title":"The Brn-3 family of POU-domain factors: Primary structure, binding specificity, and expression in subsets of retinal ganglion cells and somatosensory neurons","volume":"15","author":"Xiang","year":"1995","journal-title":"J. Neurosci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/0014-4886(74)90119-8","article-title":"Vinblastine-induced blockage of orthograde and retrograde axonal transport of protein in retinal ganglion cells","volume":"45","author":"Bunt","year":"1974","journal-title":"Exp. Neurol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/0306-4522(81)90174-3","article-title":"Evidence for an amacrine cell system in the ganglion cell layer of the rat retina","volume":"6","author":"Perry","year":"1981","journal-title":"Neuroscience"},{"key":"ref_65","first-page":"1387","article-title":"Evaluation of the percentage of ganglion cells in the ganglion cell layer of the rodent retina","volume":"19","author":"Schlamp","year":"2013","journal-title":"Mol. Vis."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"6095","DOI":"10.1167\/iovs.15-17195","article-title":"Long-Term Effect of Optic Nerve Axotomy on the Retinal Ganglion Cell Layer","volume":"56","year":"2015","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1801053","DOI":"10.1002\/mnfr.201801053","article-title":"Visual Performance in the \u201cReal World\u201d: Contrast Sensitivity, Visual Acuity, and Effects of Macular Carotenoids","volume":"63","author":"Roark","year":"2019","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Diez-Roux, G., Banfi, S., Sultan, M., Geffers, L., Anand, S., Rozado, D., Magen, A., Canidio, E., Pagani, M., and Peluso, I. (2011). A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol., 9.","DOI":"10.1371\/journal.pbio.1000582"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1016\/j.neuron.2009.01.020","article-title":"Distinct roles of transcription factors brn3a and brn3b in controlling the development, morphology, and function of retinal ganglion cells","volume":"61","author":"Badea","year":"2009","journal-title":"Neuron"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"10384","DOI":"10.1523\/JNEUROSCI.5858-12.2013","article-title":"RNA-binding protein Hermes\/RBPMS inversely affects synapse density and axon arbor formation in retinal ganglion cells in vivo","volume":"33","author":"Maurus","year":"2013","journal-title":"J. Neurosci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0925-4773(02)00459-8","article-title":"Identification of new Xlsirt family members in the Xenopus laevis oocyte","volume":"120","author":"Zearfoss","year":"2003","journal-title":"Mech. Dev."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1101\/gr.132159.111","article-title":"The GENCODE v7 catalog of human long noncoding RNAs: Analysis of their gene structure, evolution, and expression","volume":"22","author":"Derrien","year":"2012","journal-title":"Genome Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"e46327","DOI":"10.7554\/eLife.46327","article-title":"Identification of RBPMS as a mammalian smooth muscle master splicing regulator via proximity of its gene with super-enhancers","volume":"8","author":"Gooding","year":"2019","journal-title":"eLife"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.ydbio.2004.03.039","article-title":"Genetic analysis of the homeodomain transcription factor Chx10 in the retina using a novel multifunctional BAC transgenic mouse reporter","volume":"271","author":"Rowan","year":"2004","journal-title":"Dev. Biol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.ydbio.2003.08.005","article-title":"Math5 determines the competence state of retinal ganglion cell progenitors","volume":"264","author":"Yang","year":"2003","journal-title":"Dev. Biol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.ydbio.2012.03.006","article-title":"Math5 defines the ganglion cell competence state in a subpopulation of retinal progenitor cells exiting the cell cycle","volume":"365","author":"Brzezinski","year":"2012","journal-title":"Dev. Biol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1002\/(SICI)1526-968X(200002)26:2<130::AID-GENE9>3.0.CO;2-I","article-title":"Retina- and ventral forebrain-specific Cre recombinase activity in transgenic mice","volume":"26","author":"Furuta","year":"2000","journal-title":"Genesis"},{"key":"ref_78","first-page":"4522","article-title":"Retinal ganglion cells with recombinase activity in mice with engineered Cre in the vGluT3 gene","volume":"61","author":"Chen","year":"2020","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Warthi, G.D., Faulkner, J.L., Doja, J., Ghanam, A.R., Gao, P., Yang, A.C., Slivano, O.J., Barris, C.T., Kress, T.C., and Zawieja, S.D. (2022). An Inducible Cre Mouse with Preferential Activity in Vascular Smooth Muscle Evades a Previously Lethal Intestinal Phenotype. bioRxiv.","DOI":"10.1101\/2022.02.03.479061"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/15\/1951\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:20:44Z","timestamp":1760127644000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/15\/1951"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,27]]},"references-count":79,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["cells12151951"],"URL":"https:\/\/doi.org\/10.3390\/cells12151951","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,27]]}}}