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Veronesi","award":["nosiricaten2022"],"award-info":[{"award-number":["nosiricaten2022"]}]},{"name":"Daniele Lana is a recipient of a fellowship from Fondazione U. Veronesi","award":["zecchiricaten2022"],"award-info":[{"award-number":["zecchiricaten2022"]}]},{"name":"Daniele Lana is a recipient of a fellowship from Fondazione U. Veronesi","award":["bucciantiniricaten2022"],"award-info":[{"award-number":["bucciantiniricaten2022"]}]},{"name":"Daniele Lana is a recipient of a fellowship from Fondazione U. Veronesi","award":["giovanniniricaten2022"],"award-info":[{"award-number":["giovanniniricaten2022"]}]},{"name":"Daniele Lana is a recipient of a fellowship from Fondazione U. Veronesi","award":["Cooperatio 36 funding scheme"],"award-info":[{"award-number":["Cooperatio 36 funding scheme"]}]},{"name":"Daniele Lana is a recipient of a fellowship from Fondazione U. Veronesi","award":["PROPREBIOAD"],"award-info":[{"award-number":["PROPREBIOAD"]}]},{"name":"Daniele Lana is a recipient of a fellowship from Fondazione U. Veronesi","award":["ZECCHICRF2018"],"award-info":[{"award-number":["ZECCHICRF2018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The term neuroinflammation defines the reactions of astrocytes and microglia to alterations in homeostasis in the diseased central nervous system (CNS), the exacerbation of which contributes to the neurodegenerative effects of Alzheimer\u2019s disease (AD). Local environmental conditions, such as the presence of proinflammatory molecules, mechanical properties of the extracellular matrix (ECM), and local cell\u2013cell interactions, are determinants of glial cell phenotypes. In AD, the load of the cytotoxic\/proinflammatory amyloid \u03b2 (A\u03b2) peptide is a microenvironmental component increasingly growing in the CNS, imposing time-evolving challenges on resident cells. This study aimed to investigate the temporal and spatial variations of the effects produced by this process on astrocytes and microglia, either directly or by interfering in their interactions. Ex vivo confocal analyses of hippocampal sections from the mouse model TgCRND8 at different ages have shown that overproduction of A\u03b2 peptide induced early and time-persistent disassembly of functional astroglial syncytium and promoted a senile phenotype of reactive microglia, hindering A\u03b2 clearance. In the late stages of the disease, these patterns were altered in the presence of A\u03b2-plaques, surrounded by typically reactive astrocytes and microglia. Morphofunctional characterization of peri-plaque gliosis revealed a direct contribution of astrocytes in plaque buildup that might result in shielding A\u03b2-peptide cytotoxicity and, as a side effect, in exacerbating neuroinflammation.<\/jats:p>","DOI":"10.3390\/cells12182258","type":"journal-article","created":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T21:34:12Z","timestamp":1694554452000},"page":"2258","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Morphofunctional Investigation in a Transgenic Mouse Model of Alzheimer\u2019s Disease: Non-Reactive Astrocytes Are Involved in A\u03b2 Load and Reactive Astrocytes in Plaque Build-Up"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5193-0019","authenticated-orcid":false,"given":"Daniele","family":"Lana","sequence":"first","affiliation":[{"name":"Department of Health Sciences, University of Florence, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3179-0706","authenticated-orcid":false,"given":"Jacopo Junio Valerio","family":"Branca","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Delfino","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Florence, 50121 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4563-0420","authenticated-orcid":false,"given":"Maria Grazia","family":"Giovannini","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, University of Florence, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiorella","family":"Casamenti","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Psychology, Drug Research and Child Health, University of Florence, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Nardiello","sequence":"additional","affiliation":[{"name":"General Laboratory, Careggi University Hospital, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5243-9301","authenticated-orcid":false,"given":"Monica","family":"Bucciantini","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Biomedical Sciences, University of Florence, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Stefani","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Biomedical Sciences, University of Florence, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1941-2972","authenticated-orcid":false,"given":"Petr","family":"Zach","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Third Faculty of Medicine, Charles University, 100 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5932-7118","authenticated-orcid":false,"given":"Sandra","family":"Zecchi-Orlandini","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Nosi","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy"},{"name":"DMSC Imaging Platform, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0304-3940(87)90696-3","article-title":"Reactive Microglia in Patients with Senile Dementia of the Alzheimer Type Are Positive for the Histocompatibility Glycoprotein HLA-DR","volume":"79","author":"McGeer","year":"1987","journal-title":"Neurosci. 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