{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T10:14:50Z","timestamp":1790763290548,"version":"4.1.0"},"reference-count":48,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Sorbonne Universit\u00e9-UPMC","award":["ANR-19-CE16-0005"],"award-info":[{"award-number":["ANR-19-CE16-0005"]}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-19-CE16-0005"],"award-info":[{"award-number":["ANR-19-CE16-0005"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"name":"DIM NeRF","award":["ANR-19-CE16-0005"],"award-info":[{"award-number":["ANR-19-CE16-0005"]}]},{"name":"FRC\/Rotary \u2018Espoir en t\u00eate\u2019","award":["ANR-19-CE16-0005"],"award-info":[{"award-number":["ANR-19-CE16-0005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Na-K-2Cl cotransporter 1 (NKCC1) regulates chloride influx in neurons and thereby GABAA receptor activity in normal and pathological conditions. Here, we characterized in hippocampal neurons the membrane expression, distribution and dynamics of exogenous NKCC1a and NKCC1b isoforms and compared them to those of the chloride extruder K-Cl cotransporter 2 (KCC2). We found that NKCC1a and NKCC1b behave quite similarly. NKCC1a\/1b but not KCC2 are present along the axon initial segment where they are confined. Moreover, NKCC1a\/1b are detected in the somato-dendritic compartment at a lower level than KCC2, where they form fewer, smaller and less compact clusters at perisynaptic and extrasynaptic sites. Interestingly, ~60% of dendritic clusters of NKCC1a\/1b are colocalized with KCC2. They are larger and brighter than those devoid of KCC2, suggesting a particular NKCC1a\/1b-KCC2 relationship. In agreement with the reduced dendritic clustering of NKCC1a\/1b compared with that of KCC2, NKCC1a\/1b are more mobile on the dendrite than KCC2, suggesting weaker cytoskeletal interaction. NKCC1a\/b are confined to endocytic zones, where they spend more time than KCC2. However, they spend less time in these compartments than at the synapses, suggesting that they can rapidly leave endocytic zones to increase the membrane pool, which can happen in pathological conditions. Thus, NKCC1a\/b have different membrane dynamics and clustering from KCC2, which helps to explain their low level in the neuronal membrane, while allowing a rapid increase in the membrane pool under pathological conditions.<\/jats:p>","DOI":"10.3390\/cells12192363","type":"journal-article","created":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T08:55:01Z","timestamp":1695718501000},"page":"2363","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["NKCC1 and KCC2 Chloride Transporters Have Different Membrane Dynamics on the Surface of Hippocampal Neurons"],"prefix":"10.3390","volume":"12","author":[{"given":"Erwan","family":"Pol","sequence":"first","affiliation":[{"name":"Institut du Fer \u00e0 Moulin, Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) UMR-S 1270, Sorbonne Universit\u00e9, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"C\u00f4me","sequence":"additional","affiliation":[{"name":"Institut du Fer \u00e0 Moulin, Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) UMR-S 1270, Sorbonne Universit\u00e9, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaha","family":"Merlaud","sequence":"additional","affiliation":[{"name":"Institut du Fer \u00e0 Moulin, Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) UMR-S 1270, Sorbonne Universit\u00e9, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9811-7260","authenticated-orcid":false,"given":"Juliette","family":"Gouhier","sequence":"additional","affiliation":[{"name":"Institut du Fer \u00e0 Moulin, Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) UMR-S 1270, Sorbonne Universit\u00e9, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9681-0491","authenticated-orcid":false,"given":"Marion","family":"Russeau","sequence":"additional","affiliation":[{"name":"Institut du Fer \u00e0 Moulin, Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) UMR-S 1270, Sorbonne Universit\u00e9, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Scotto-Lomassese","sequence":"additional","affiliation":[{"name":"Institut du Fer \u00e0 Moulin, Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) UMR-S 1270, Sorbonne Universit\u00e9, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imane","family":"Moutkine","sequence":"additional","affiliation":[{"name":"Institut du Fer \u00e0 Moulin, Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) UMR-S 1270, Sorbonne Universit\u00e9, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6423-2658","authenticated-orcid":false,"given":"Xavier","family":"Marques","sequence":"additional","affiliation":[{"name":"Institut du Fer \u00e0 Moulin, Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) UMR-S 1270, Sorbonne Universit\u00e9, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9927-0945","authenticated-orcid":false,"given":"Sabine","family":"L\u00e9vi","sequence":"additional","affiliation":[{"name":"Institut du Fer \u00e0 Moulin, Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) UMR-S 1270, Sorbonne Universit\u00e9, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1038\/nrn3819","article-title":"Cation-chloride cotransporters in neuronal development, plasticity and disease","volume":"15","author":"Kaila","year":"2014","journal-title":"Nat. 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