{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:18:53Z","timestamp":1792174733954,"version":"4.4.0"},"reference-count":86,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T00:00:00Z","timestamp":1698192000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Health (NIH)","award":["EY025377"],"award-info":[{"award-number":["EY025377"]}]},{"name":"National Institute of Health (NIH)","award":["EY029829"],"award-info":[{"award-number":["EY029829"]}]},{"name":"National Institute of Health (NIH)","award":["EY013431"],"award-info":[{"award-number":["EY013431"]}]},{"name":"National Institute of Health (NIH)","award":["EY031377"],"award-info":[{"award-number":["EY031377"]}]},{"name":"Board of Governors Regenerative Medicine Institute","award":["EY025377"],"award-info":[{"award-number":["EY025377"]}]},{"name":"Board of Governors Regenerative Medicine Institute","award":["EY029829"],"award-info":[{"award-number":["EY029829"]}]},{"name":"Board of Governors Regenerative Medicine Institute","award":["EY013431"],"award-info":[{"award-number":["EY013431"]}]},{"name":"Board of Governors Regenerative Medicine Institute","award":["EY031377"],"award-info":[{"award-number":["EY031377"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Epithelial and stromal\/mesenchymal limbal stem cells contribute to corneal homeostasis and cell renewal. Extracellular vesicles (EVs), including exosomes (Exos), can be paracrine mediators of intercellular communication. Previously, we described cargos and regulatory roles of limbal stromal cell (LSC)-derived Exos in non-diabetic (N) and diabetic (DM) limbal epithelial cells (LECs). Presently, we quantify the miRNA and proteome profiles of human LEC-derived Exos and their regulatory roles in N- and DM-LSC. We revealed some miRNA and protein differences in DM vs. N-LEC-derived Exos\u2019 cargos, including proteins involved in Exo biogenesis and packaging that may affect Exo production and ultimately cellular crosstalk and corneal function. Treatment by N-Exos, but not by DM-Exos, enhanced wound healing in cultured N-LSCs and increased proliferation rates in N and DM LSCs vs. corresponding untreated (control) cells. N-Exos-treated LSCs reduced the keratocyte markers ALDH3A1 and lumican and increased the MSC markers CD73, CD90, and CD105 vs. control LSCs. These being opposite to the changes quantified in wounded LSCs. Overall, N-LEC Exos have a more pronounced effect on LSC wound healing, proliferation, and stem cell marker expression than DM-LEC Exos. This suggests that regulatory miRNA and protein cargo differences in DM- vs. N-LEC-derived Exos could contribute to the disease state.<\/jats:p>","DOI":"10.3390\/cells12212524","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T02:40:07Z","timestamp":1698288007000},"page":"2524","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["MicroRNA and Protein Cargos of Human Limbal Epithelial Cell-Derived Exosomes and Their Regulatory Roles in Limbal Stromal Cells of Diabetic and Non-Diabetic Corneas"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3149-7764","authenticated-orcid":false,"given":"Nagendra","family":"Verma","sequence":"first","affiliation":[{"name":"Eye Program, Board of Governors Regenerative Medicine Institute, Cedars Sinai Medical Center, 8700 Beverly Boulevard, AHSP-A8104, Los Angeles, CA 90048, USA"},{"name":"Departments of Biomedical Sciences, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Drirh","family":"Khare","sequence":"additional","affiliation":[{"name":"Eye Program, Board of Governors Regenerative Medicine Institute, Cedars Sinai Medical Center, 8700 Beverly Boulevard, AHSP-A8104, Los Angeles, CA 90048, USA"},{"name":"Departments of Biomedical Sciences, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA"},{"name":"Division of Pediatric Blood and Marrow Transplantation & Cellular Therapy, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam J.","family":"Poe","sequence":"additional","affiliation":[{"name":"Eye Program, Board of Governors Regenerative Medicine Institute, Cedars Sinai Medical Center, 8700 Beverly Boulevard, AHSP-A8104, Los Angeles, CA 90048, USA"},{"name":"Departments of Biomedical Sciences, Cedars Sinai Medical Center, Los Angeles, CA 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Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Fealy","sequence":"additional","affiliation":[{"name":"Eye Program, Board of Governors Regenerative Medicine Institute, Cedars Sinai Medical Center, 8700 Beverly Boulevard, AHSP-A8104, Los Angeles, CA 90048, USA"},{"name":"Departments of Biomedical Sciences, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaghaiegh","family":"Ebrahimi","sequence":"additional","affiliation":[{"name":"Eye Program, Board of Governors Regenerative Medicine Institute, Cedars Sinai Medical Center, 8700 Beverly Boulevard, AHSP-A8104, Los Angeles, CA 90048, USA"},{"name":"Departments of Biomedical Sciences, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Odelia","family":"Shadrokh","sequence":"additional","affiliation":[{"name":"Eye Program, Board of Governors Regenerative Medicine Institute, Cedars Sinai Medical Center, 8700 Beverly Boulevard, AHSP-A8104, Los Angeles, CA 90048, USA"},{"name":"Departments of Biomedical Sciences, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Ying","family":"Song","sequence":"additional","affiliation":[{"name":"Genomics Core, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chintda","family":"Santiskulvong","sequence":"additional","affiliation":[{"name":"Genomics Core, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitra","family":"Mastali","sequence":"additional","affiliation":[{"name":"Advanced Clinical Biosystems Research Institute, The Smidt Heart Institute, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Parker","sequence":"additional","affiliation":[{"name":"Advanced Clinical Biosystems Research Institute, The Smidt Heart Institute, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandr","family":"Stotland","sequence":"additional","affiliation":[{"name":"Advanced Clinical Biosystems Research Institute, The Smidt Heart Institute, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer E.","family":"Van Eyk","sequence":"additional","affiliation":[{"name":"Advanced Clinical Biosystems Research Institute, The Smidt Heart Institute, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2398-5319","authenticated-orcid":false,"given":"Alexander 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