{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T19:27:40Z","timestamp":1789154860918,"version":"build-2803163510"},"reference-count":35,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT159687"],"award-info":[{"award-number":["PJT159687"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Synchronized contractions of cardiomyocytes within the heart are tightly coupled to electrical stimulation known as excitation-contraction coupling. Calcium plays a key role in this process and dysregulated calcium handling can significantly impair cardiac function and lead to the development of cardiomyopathies and heart failure. Here, we describe a method and analytical technique to study myofilament-localized calcium signaling using the intensity-based fluorescent biosensor, RGECO-TnT. Dilated cardiomyopathy is a heart muscle disease that negatively impacts the heart\u2019s contractile function following dilatation of the left ventricle. We demonstrate how this biosensor can be used to characterize 2D hiPSC-CMs monolayers generated from a healthy control subject compared to two patients diagnosed with dilated cardiomyopathy. Lastly, we provide a step-by-step guide for single-cell data analysis and describe a custom Transient Analysis application, specifically designed to quantify features of calcium transients. All in all, we explain how this analytical approach can be applied to phenotype hiPSC-CM behaviours and stratify patient responses to identify perturbations in calcium signaling.<\/jats:p>","DOI":"10.3390\/cells12212526","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T06:20:16Z","timestamp":1698301216000},"page":"2526","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Measuring Single-Cell Calcium Dynamics Using a Myofilament-Localized Optical Biosensor in hiPSC-CMs Derived from DCM Patients"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5580-6867","authenticated-orcid":false,"given":"Cara","family":"Hawey","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Therapeutics, McGill University, Montr\u00e9al, QC H3G 1Y6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyla","family":"Bourque","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, McGill University, Montr\u00e9al, QC H3G 1Y6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karima","family":"Alim","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, McGill University, Montr\u00e9al, QC H3G 1Y6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2843-1470","authenticated-orcid":false,"given":"Ida","family":"Derish","sequence":"additional","affiliation":[{"name":"Research Institute, McGull University Hospital Centre, 1001 Decarie Blvd, Montr\u00e9al, QC H4A 3J1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise","family":"Rody","sequence":"additional","affiliation":[{"name":"Research Institute, McGull University Hospital Centre, 1001 Decarie Blvd, Montr\u00e9al, QC H4A 3J1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kashif","family":"Khan","sequence":"additional","affiliation":[{"name":"Research Institute, McGull University Hospital Centre, 1001 Decarie Blvd, Montr\u00e9al, QC H4A 3J1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Gendron","sequence":"additional","affiliation":[{"name":"Research Institute, McGull University Hospital Centre, 1001 Decarie Blvd, Montr\u00e9al, QC H4A 3J1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renzo","family":"Cecere","sequence":"additional","affiliation":[{"name":"Research Institute, McGull University Hospital Centre, 1001 Decarie Blvd, Montr\u00e9al, QC H4A 3J1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Giannetti","sequence":"additional","affiliation":[{"name":"Research Institute, McGull University Hospital Centre, 1001 Decarie Blvd, Montr\u00e9al, QC H4A 3J1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terence E.","family":"H\u00e9bert","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, McGill University, Montr\u00e9al, QC H3G 1Y6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1093\/hmg\/ddv468","article-title":"Modeling structural and functional deficiencies of RBM20 familial dilated cardiomyopathy using human induced pluripotent stem cells","volume":"25","author":"Wyles","year":"2016","journal-title":"Hum. 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