{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T13:59:51Z","timestamp":1784210391320,"version":"3.55.0"},"reference-count":73,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T00:00:00Z","timestamp":1699228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R44GM108150"],"award-info":[{"award-number":["R44GM108150"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01OD016575"],"award-info":[{"award-number":["R01OD016575"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Spermatogonial stem cell (SSC) transplantation into the testis of a germ cell (GC)-depleted surrogate allows transmission of donor genotype via donor-derived sperm produced by the recipient. Transplantation of gene-edited SSCs provides an approach to propagate gene-edited large animal models. DAZL is a conserved RNA-binding protein important for GC development, and DAZL knockout (KO) causes defects in GC commitment and differentiation. We characterized DAZL-KO pigs as SSC transplantation recipients. While there were GCs in 1-week-old (wko) KO, complete GC depletion was observed by 10 wko. Donor GCs were transplanted into 18 DAZL-KO recipients at 10\u201313 wko. At sexual maturity, semen and testes were evaluated for transplantation efficiency and spermatogenesis. Approximately 22% of recipient seminiferous tubules contained GCs, including elongated spermatids and proliferating spermatogonia. The ejaculate of 89% of recipients contained sperm, exclusively from donor origin. However, sperm concentration was lower than the wild-type range. Testicular protein expression and serum hormonal levels were comparable between DAZL-KO and wild-type. Intratesticular testosterone and Leydig cell volume were increased, and Leydig cell number decreased in transplanted DAZL-KO testis compared to wild-type. In summary, DAZL-KO pigs support donor-derived spermatogenesis following SSC transplantation, but low spermatogenic efficiency currently limits their use for the production of offspring.<\/jats:p>","DOI":"10.3390\/cells12212582","type":"journal-article","created":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T08:15:56Z","timestamp":1699258556000},"page":"2582","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["DAZL Knockout Pigs as Recipients for Spermatogonial Stem Cell Transplantation"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8170-6384","authenticated-orcid":false,"given":"Nathalia L. 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Fertil. Dev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1071\/RD07036","article-title":"Germ cell transplantation for the propagation of companion animals, non-domestic and endangered species","volume":"19","author":"Dobrinski","year":"2007","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1071\/RD17418","article-title":"Recent advances for spermatogonial stem cell transplantation in livestock","volume":"30","author":"Oatley","year":"2018","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"hoaa016","DOI":"10.1093\/hropen\/hoaa016","article-title":"Fertility preservation in boys: Recent developments and new insights","volume":"2020","author":"Goossens","year":"2020","journal-title":"Hum. Reprod. Open"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"55","DOI":"10.12703\/r\/10-55","article-title":"Recent advances: Fertility preservation and fertility restoration options for males and females","volume":"10","author":"Doungkamchan","year":"2021","journal-title":"Fac. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Zhao, X., Wan, W., Zhang, X., Wu, Z., and Yang, H. (2021). Spermatogonial stem cell transplantation in large animals. Animals, 11.","DOI":"10.3390\/ani11040918"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Zhang, W., Nie, R., Cai, Y., Xie, W., and Zou, K. (2022). Progress in germline stem cell transplantation in mammals and the potential usage. Reprod. Biol. Endocrinol., 20.","DOI":"10.1186\/s12958-022-00930-5"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1002\/j.1939-4640.2000.tb03408.x","article-title":"All you wanted to know about spermatogonia but were afraid to ask","volume":"21","author":"Russell","year":"2000","journal-title":"J. Androl."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1530\/rep.0.1210347","article-title":"Proliferation and differentiation of spermatogonial stem cells","volume":"121","year":"2001","journal-title":"Reproduction"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1093\/humupd\/dmk001","article-title":"Spermatogonial stem cells: Questions, models and perspectives","volume":"12","author":"Ehmcke","year":"2006","journal-title":"Hum. Reprod. Update"},{"key":"ref_12","first-page":"35","article-title":"Regulation of germ line stem cell homeostasis","volume":"12","author":"Garcia","year":"2015","journal-title":"Anim. Reprod."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1093\/biolre\/ioy077","article-title":"Spermatogonial stem cells","volume":"99","author":"Kubota","year":"2018","journal-title":"Biol. Reprod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1093\/humupd\/dmz006","article-title":"Spermatogonial stem cells: Updates from specification to clinical relevance","volume":"25","author":"Sharma","year":"2019","journal-title":"Hum. Reprod. Update"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"11303","DOI":"10.1073\/pnas.91.24.11303","article-title":"Germline transmission of donor haplotype following spermatogonial transplantation","volume":"91","author":"Brinster","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"11298","DOI":"10.1073\/pnas.91.24.11298","article-title":"Spermatogenesis following male germ-cell transplantation","volume":"91","author":"Brinster","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1095\/biolreprod60.6.1429","article-title":"Pattern and kinetics of mouse donor spermatogonial stem cell colonization in recipient testes","volume":"60","author":"Nagano","year":"1999","journal-title":"Biol. Reprod."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1095\/biolreprod66.1.21","article-title":"Germ cell transplantation in pigs","volume":"66","author":"Honaramooz","year":"2002","journal-title":"Biol. Reprod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/j.stem.2012.07.017","article-title":"Spermatogonial stem cell transplantation into rhesus testes regenerates spermatogenesis producing functional sperm","volume":"11","author":"Hermann","year":"2012","journal-title":"Cell Stem Cell"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1016\/j.stem.2021.03.016","article-title":"Transient suppression of transplanted spermatogonial stem cell differentiation restores fertility in mice","volume":"28","author":"Nakamura","year":"2021","journal-title":"Cell Stem Cell"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"16526","DOI":"10.1073\/pnas.1310478110","article-title":"Efficient nonmeiotic allele introgression in livestock using custom endonucleases","volume":"110","author":"Tan","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1093\/ilar\/ilv004","article-title":"Beyond the mouse monopoly: Studying the male germ line in domestic animal models","volume":"56","author":"Gonzalez","year":"2015","journal-title":"ILAR J."},{"key":"ref_23","first-page":"93","article-title":"Germline modification of domestic animals","volume":"12","author":"Tang","year":"2015","journal-title":"Anim. Reprod."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Whitworth, K.M., Green, J.A., Redel, B.K., Geisert, R.D., Lee, K., Telugu, B.P., Wells, K.D., and Prather, R.S. (2022). Improvements in pig agriculture through gene editing. CABI Agric. Biosci., 3.","DOI":"10.1186\/s43170-022-00111-9"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"F1","DOI":"10.1530\/REP-18-0533","article-title":"Testicular transplantation for fertility preservation: Clinical potential and current challenges","volume":"158","author":"Kilcoyne","year":"2019","journal-title":"Reproduction"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1093\/biolre\/ioac072","article-title":"Male fertility preservation and restoration strategies for patients undergoing gonadotoxic therapies","volume":"107","author":"Tran","year":"2022","journal-title":"Biol. Reprod."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.scr.2018.03.006","article-title":"Culture and transplantation of spermatogonial stem cells","volume":"29","author":"Takashima","year":"2018","journal-title":"Stem Cell Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.1007\/s10815-021-02334-7","article-title":"Current scenario and challenges ahead in application of spermatogonial stem cell technology in livestock","volume":"38","author":"Binsila","year":"2021","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1038\/71496","article-title":"Transplantation of male germ line stem cells restores fertility in infertile mice","volume":"6","author":"Ogawa","year":"2000","journal-title":"Nat. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1095\/biolreprod.103.016519","article-title":"Restoration of fertility by germ cell transplantation requires effective recipient preparation","volume":"69","author":"Brinster","year":"2003","journal-title":"Biol. Reprod."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"698","DOI":"10.2164\/jandrol.05032","article-title":"Depletion of endogenous germ cells in male pigs and goats in preparation for germ cell transplantation","volume":"26","author":"Honaramooz","year":"2005","journal-title":"J. Androl."},{"key":"ref_32","first-page":"112","article-title":"Fertility of male germline stem cells following spermatogonial transplantation in infertile mouse models","volume":"94","author":"Morimoto","year":"2016","journal-title":"Biol. Reprod."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.1111\/andr.12807","article-title":"Restoration of functional sperm production in irradiated pubertal rhesus monkeys by spermatogonial stem cell transplantation","volume":"8","author":"Shetty","year":"2020","journal-title":"Andrology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"104448","DOI":"10.1016\/j.livsci.2021.104448","article-title":"Intratesticular injection of busulfan for producing recipient male pigs for spermatogonial stem cell transplantation","volume":"245","author":"Zhang","year":"2021","journal-title":"Livest. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1093\/humrep\/17.1.55","article-title":"Germ cell transplantation into X-irradiated monkey testes","volume":"17","author":"Schlatt","year":"2002","journal-title":"Hum. Reprod."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1002\/jcp.20910","article-title":"The radiation-induced block in spermatogonial differentiation is due to damage to the somatic environment, not the germ cells","volume":"211","author":"Zhang","year":"2007","journal-title":"J. Cell. Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1095\/biolreprod.109.078279","article-title":"Irradiation enhances the efficiency of testicular germ cell transplantation in sheep","volume":"81","author":"Herrid","year":"2009","journal-title":"Biol. Reprod."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1095\/biolreprod.110.089623","article-title":"Efficient and safe recipient preparation for transplantation of mouse spermatogonial stem cells: Pretreating testes with heat shock","volume":"85","author":"Ma","year":"2011","journal-title":"Biol. Reprod."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e20200001","DOI":"10.1590\/1984-3143-ar2020-0001","article-title":"Spermatogonial stem cell survival in ram lambs following busulfan treatment","volume":"17","author":"Rasouli","year":"2020","journal-title":"Anim. Reprod."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1530\/rep.0.1260599","article-title":"Spermatogenesis in testes of Dazl null mice after transplantation of wild-type germ cells","volume":"126","author":"Rilianawati","year":"2003","journal-title":"Reproduction"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"40176","DOI":"10.1038\/srep40176","article-title":"Generation of germline ablated male pigs by CRISPR\/Cas9 editing of the NANOS2 gene","volume":"7","author":"Park","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"24195","DOI":"10.1073\/pnas.2010102117","article-title":"Donor-derived spermatogenesis following stem cell transplantation in sterile NANOS2 knockout males","volume":"117","author":"Ciccarelli","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Shah, C., VanGompel, M.J.W., Naeem, V., Chen, Y., Lee, T., Angeloni, N., Wang, Y., and Xu, E.Y. (2010). Widespread presence of human BOULE homologs among animals and conservation of their ancient reproductive function. PLoS Genet., 6.","DOI":"10.1371\/journal.pgen.1001022"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"36","DOI":"10.4161\/spmg.1.1.14659","article-title":"The roles of the DAZ family in spermatogenesis","volume":"1","author":"VanGompel","year":"2011","journal-title":"Spermatogenesis"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.7150\/ijbs.11536","article-title":"DAZ family proteins, key players for germ cell development","volume":"11","author":"Fu","year":"2015","journal-title":"Int. J. Biol. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/37987","article-title":"The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis","volume":"389","author":"Ruggiu","year":"1997","journal-title":"Nature"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.ydbio.2005.06.032","article-title":"Dazl deficiency leads to embryonic arrest of germ cell development in XY C57BL\/6 mice","volume":"288","author":"Lin","year":"2005","journal-title":"Dev. Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1093\/nsr\/nwy163","article-title":"DAZL is a master translational regulator of murine spermatogenesis","volume":"6","author":"Li","year":"2019","journal-title":"Natl. Sci. Rev."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e56523","DOI":"10.7554\/eLife.56523","article-title":"DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors","volume":"9","author":"Mikedis","year":"2020","journal-title":"Elife"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1530\/rep.0.1260589","article-title":"Absence of mDazl produces a final block on germ cell development at meiosis","volume":"126","author":"Saunders","year":"2003","journal-title":"Reproduction"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3899","DOI":"10.1093\/hmg\/ddi414","article-title":"Dazl binds in vivo to specific transcripts and can regulate the pre-meiotic translation of Mvh in germ cells","volume":"14","author":"Reynolds","year":"2005","journal-title":"Hum. Mol. Genet."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1038\/nature08562","article-title":"Human DAZL, DAZ and BOULE genes modulate primordial germ-cell and haploid gamete formation","volume":"462","author":"Kee","year":"2009","journal-title":"Nature"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"7443","DOI":"10.1073\/pnas.1104501108","article-title":"Licensing of gametogenesis, dependent on RNA binding protein DAZL, as a gateway to sexual differentiation of fetal germ cells","volume":"108","author":"Gill","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"25677","DOI":"10.1073\/pnas.1910733116","article-title":"Mammalian germ cells are determined after PGC colonization of the nascent gonad","volume":"116","author":"Nicholls","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1038\/ng0895-383","article-title":"Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA\u2013binding protein gene","volume":"10","author":"Reijo","year":"1995","journal-title":"Nat. Genet."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1093\/molehr\/7.11.1015","article-title":"Expression patterns and transcript concentrations of the autosomal DAZL gene in testes of azoospermic men","volume":"7","author":"Lin","year":"2001","journal-title":"Mol. Hum. Reprod."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Tung, J.Y., Rosen, M.P., Nelson, L.M., Turek, P.J., Witte, J.S., Cramer, D.W., Cedars, M.I., and Reijo-Pera, R.A. (2006). Novel missense mutations of the Deleted-in-AZoospermia-Like (DAZL) gene in infertile women and men. Reprod. Biol. Endocrinol., 4.","DOI":"10.1186\/1477-7827-4-40"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1080\/19396368.2017.1305466","article-title":"In silico analysis of non-synonymous single nucleotide polymorphisms in human DAZL gene associated with male infertility","volume":"63","author":"Nailwal","year":"2017","journal-title":"Syst. Biol. Reprod. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1095\/biolreprod65.3.771","article-title":"Nature of the spermatogenic arrest in Dazl\u2212\/\u2212 mice","volume":"65","author":"Saunders","year":"2001","journal-title":"Biol. Reprod."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1002\/mrd.20010","article-title":"Expression profiling of the developing testis in wild-type and Dazl knockout mice","volume":"67","author":"Maratou","year":"2004","journal-title":"Mol. Reprod. Dev."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/mrd.23443","article-title":"Testes of DAZL null neonatal sheep lack prospermatogonia but maintain normal somatic cell morphology and marker expression","volume":"88","author":"McLean","year":"2021","journal-title":"Mol. Reprod. Dev."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1095\/biolreprod.112.104422","article-title":"Viral transduction of male germline stem cells results in transgene transmission after germ cell transplantation in pigs","volume":"88","author":"Zeng","year":"2013","journal-title":"Biol. Reprod."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1002\/mrd.22961","article-title":"TALEN-mediated gene targeting in porcine spermatogonia","volume":"85","author":"Tang","year":"2018","journal-title":"Mol. Reprod. Dev."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.theriogenology.2009.12.014","article-title":"Postnatal somatic cell proliferation and seminiferous tubule maturation in pigs: A non-random event","volume":"74","author":"Avelar","year":"2010","journal-title":"Theriogenology"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.3389\/fgene.2020.627673","article-title":"Targeted gene editing in porcine spermatogonia","volume":"11","author":"Webster","year":"2021","journal-title":"Front. Genet."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"6186","DOI":"10.1073\/pnas.111158198","article-title":"Remodeling of the postnatal mouse testis is accompanied by dramatic changes in stem cell number and niche accessibility","volume":"98","author":"Shinohara","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1111\/j.1439-0531.2006.00651.x","article-title":"Transplantation of normal boar testicular cells resulted in complete focal spermatogenesis in a boar affected by the immotile short-tail sperm defect","volume":"41","author":"Mikkola","year":"2006","journal-title":"Reprod. Domest. Anim."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Oatley, J., and Griswold, M. (2017). The Biology of Mammalian Spermatogonia, Springer.","DOI":"10.1007\/978-1-4939-7505-1"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.semcdb.2014.02.012","article-title":"The regulation of spermatogenesis by androgens","volume":"30","author":"Smith","year":"2014","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"R151","DOI":"10.1530\/REP-16-0588","article-title":"Role of the testis interstitial compartment in spermatogonial stem cell function","volume":"153","author":"Potter","year":"2017","journal-title":"Reproduction"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Grynberg, M., and Patrizio, P. (2022). Female and Male Fertility Preservation, Springer International Publishing.","DOI":"10.1007\/978-3-030-47767-7"},{"key":"ref_72","unstructured":"Cheng, C.Y. (2012). Biology and Regulation of Blood Tissue Barriers, Landes Bioscience and Springer Science."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/andr.12571","article-title":"Effect of hormone modulations on donor-derived spermatogenesis or colonization after syngeneic and xenotransplantation in mice","volume":"7","author":"Shetty","year":"2019","journal-title":"Andrology"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/21\/2582\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:18:09Z","timestamp":1760131089000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/21\/2582"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,6]]},"references-count":73,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2023,11]]}},"alternative-id":["cells12212582"],"URL":"https:\/\/doi.org\/10.3390\/cells12212582","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,6]]}}}