{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:57:25Z","timestamp":1790143045882,"version":"4.0.1"},"reference-count":200,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T00:00:00Z","timestamp":1699401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["174ISSFPP"],"award-info":[{"award-number":["174ISSFPP"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["EP\/Y030206\/1"],"award-info":[{"award-number":["EP\/Y030206\/1"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["EP\/V029983\/1"],"award-info":[{"award-number":["EP\/V029983\/1"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"EPSRC","doi-asserted-by":"publisher","award":["174ISSFPP"],"award-info":[{"award-number":["174ISSFPP"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"EPSRC","doi-asserted-by":"publisher","award":["EP\/Y030206\/1"],"award-info":[{"award-number":["EP\/Y030206\/1"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"EPSRC","doi-asserted-by":"publisher","award":["EP\/V029983\/1"],"award-info":[{"award-number":["EP\/V029983\/1"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"UK Research and Innovation (UKRI)","doi-asserted-by":"publisher","award":["174ISSFPP"],"award-info":[{"award-number":["174ISSFPP"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"UK Research and Innovation (UKRI)","doi-asserted-by":"publisher","award":["EP\/Y030206\/1"],"award-info":[{"award-number":["EP\/Y030206\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"UK Research and Innovation (UKRI)","doi-asserted-by":"publisher","award":["EP\/V029983\/1"],"award-info":[{"award-number":["EP\/V029983\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Traumatic brain injury (TBI) affects millions of people of all ages around the globe. TBI is notoriously hard to diagnose at the point of care, resulting in incorrect patient management, avoidable death and disability, long-term neurodegenerative complications, and increased costs. It is vital to develop timely, alternative diagnostics for TBI to assist triage and clinical decision-making, complementary to current techniques such as neuroimaging and cognitive assessment. These could deliver rapid, quantitative TBI detection, by obtaining information on biochemical changes from patient\u2019s biofluids. If available, this would reduce mis-triage, save healthcare providers costs (both over- and under-triage are expensive) and improve outcomes by guiding early management. Herein, we utilize Raman spectroscopy-based detection to profile a panel of 18 raw (human, animal, and synthetically derived) TBI-indicative biomarkers (N-acetyl-aspartic acid (NAA), Ganglioside, Glutathione (GSH), Neuron Specific Enolase (NSE), Glial Fibrillary Acidic Protein (GFAP), Ubiquitin C-terminal Hydrolase L1 (UCHL1), Cholesterol, D-Serine, Sphingomyelin, Sulfatides, Cardiolipin, Interleukin-6 (IL-6), S100B, Galactocerebroside, Beta-D-(+)-Glucose, Myo-Inositol, Interleukin-18 (IL-18), Neurofilament Light Chain (NFL)) and their aqueous solution. The subsequently derived unique spectral reference library, exploiting four excitation lasers of 514, 633, 785, and 830 nm, will aid the development of rapid, non-destructive, and label-free spectroscopy-based neuro-diagnostic technologies. These biomolecules, released during cellular damage, provide additional means of diagnosing TBI and assessing the severity of injury. The spectroscopic temporal profiles of the studied biofluid neuro-markers are classed according to their acute, sub-acute, and chronic temporal injury phases and we have further generated detailed peak assignment tables for each brain-specific biomolecule within each injury phase. The intensity ratios of significant peaks, yielding the combined unique spectroscopic barcode for each brain-injury marker, are compared to assess variance between lasers, with the smallest variance found for UCHL1 (\u03c32 = 0.000164) and the highest for sulfatide (\u03c32 = 0.158). Overall, this work paves the way for defining and setting the most appropriate diagnostic time window for detection following brain injury. Further rapid and specific detection of these biomarkers, from easily accessible biofluids, would not only enable the triage of TBI, predict outcomes, indicate the progress of recovery, and save healthcare providers costs, but also cement the potential of Raman-based spectroscopy as a powerful tool for neurodiagnostics.<\/jats:p>","DOI":"10.3390\/cells12222589","type":"journal-article","created":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T07:02:05Z","timestamp":1699426925000},"page":"2589","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Raman Spectroscopy Spectral Fingerprints of Biomarkers of Traumatic Brain Injury"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8826-7057","authenticated-orcid":false,"given":"Georgia","family":"Harris","sequence":"first","affiliation":[{"name":"Advanced Nanomaterials Structures and Applications Laboratories, School of Chemical Engineering, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clarissa A.","family":"Stickland","sequence":"additional","affiliation":[{"name":"Advanced Nanomaterials Structures and Applications Laboratories, School of Chemical Engineering, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Lim","sequence":"additional","affiliation":[{"name":"Advanced Nanomaterials Structures and Applications Laboratories, School of Chemical Engineering, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1014-4724","authenticated-orcid":false,"given":"Pola","family":"Goldberg Oppenheimer","sequence":"additional","affiliation":[{"name":"Advanced Nanomaterials Structures and Applications Laboratories, School of Chemical Engineering, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK"},{"name":"Institute of Healthcare Technologies, Mindelsohn Way, Birmingham B15 2TH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2878","DOI":"10.1007\/s00415-019-09541-4","article-title":"Recent advances in traumatic brain injury","volume":"266","author":"Khellaf","year":"2019","journal-title":"J. Neurol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.mcna.2019.11.001","article-title":"Traumatic Brain Injury: An Overview of Epidemiology, Pathophysiology, and Medical Management","volume":"104","author":"Capizzi","year":"2020","journal-title":"Med. Clin. N. Am."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1080\/14737159.2018.1428089","article-title":"An update on diagnostic and prognostic biomarkers for traumatic brain injury","volume":"18","author":"Wang","year":"2018","journal-title":"Expert Rev. Mol. Diagn."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e0294-16","DOI":"10.1523\/ENEURO.0294-16.2016","article-title":"Biomarkers of traumatic brain injury: Temporal changes in body fluids","volume":"3","author":"Adrian","year":"2016","journal-title":"eNeuro"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ercole, A., Thelin, E.P., Holst, A., Bellander, B.M., and Nelson, D.W. (2016). Kinetic modelling of serum S100b after traumatic brain injury. BMC Neurol., 16.","DOI":"10.1186\/s12883-016-0614-3"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1007\/s11910-020-01076-w","article-title":"Recent Advances in Blood-Based Biomarkers of Remote Combat-Related Traumatic Brain Injury","volume":"20","author":"Lippa","year":"2020","journal-title":"Curr. Neurol. Neurosci. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1002\/prca.200800011","article-title":"Neuroproteomics and systems biology-based discovery of protein biomarkers for traumatic brain injury and clinical validation","volume":"2","author":"Kobeissy","year":"2008","journal-title":"Proteom.\u2014Clin. Appl."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1080\/02699052.2017.1357836","article-title":"Biofluid biomarkers of traumatic brain injury","volume":"31","author":"Agoston","year":"2017","journal-title":"Brain Inj."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e623","DOI":"10.1212\/WNL.0000000000009985","article-title":"Time course and diagnostic utility of NfL, tau, GFAP, and UCH-L1 in subacute and chronic TBI","volume":"95","author":"Shahim","year":"2020","journal-title":"Neurology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1001\/jamaneurol.2017.0655","article-title":"Comparing plasma phospho tau, total tau, and phospho tau\u2013total tau ratio as acute and chronic traumatic brain injury biomarkers","volume":"74","author":"Rubenstein","year":"2017","journal-title":"JAMA Neurol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"431","DOI":"10.3389\/fnins.2019.00431","article-title":"Consolidated biochemical profile of subacute stage traumatic brain injury in early development","volume":"13","author":"Chitturi","year":"2019","journal-title":"Front. Neurosci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.3171\/2017.10.JNS17352","article-title":"Estimating the global incidence of traumatic brain injury","volume":"130","author":"Dewan","year":"2019","journal-title":"J. Neurosurg."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1016\/S1474-4422(14)70120-6","article-title":"The Glasgow Coma Scale at 40 years: Standing the test of time","volume":"13","author":"Teasdale","year":"2014","journal-title":"Lancet Neurol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1197\/j.aem.2005.07.038","article-title":"Accuracy of mild traumatic brain injury case ascertainment using ICD-9 codes","volume":"13","author":"Bazarian","year":"2006","journal-title":"Acad. Emerg. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2514","DOI":"10.1089\/neu.2019.6762","article-title":"Biomarkers for Traumatic Brain Injury: Data Standards and Statistical Considerations","volume":"38","author":"Huie","year":"2021","journal-title":"J. Neurotrauma"},{"key":"ref_16","first-page":"e40417","article-title":"Blood-Based Biomarkers in Traumatic Brain Injury: A Narrative Review with Implications for the Legal System","volume":"15","author":"McBride","year":"2023","journal-title":"Cureus"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Mavroudis, I., Petridis, F., Balmus, I.M., Ciobica, A., Gorgan, D.L., and Luca, A.C. (2023). Review on the Role of Salivary Biomarkers in the Diagnosis of Mild Traumatic Brain Injury and Post-Concussion Syndrome. Diagnostics, 13.","DOI":"10.3390\/diagnostics13081367"},{"key":"ref_18","first-page":"88","article-title":"The diagnostic accuracy of serum and urinary S100B protein in children and adolescents with mild traumatic brain injury","volume":"73","author":"Mozafari","year":"2019","journal-title":"N. Z. J. Med. Lab. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.mcn.2015.02.003","article-title":"Fluid markers of traumatic brain injury","volume":"66","author":"Zetterberg","year":"2015","journal-title":"Mol. Cell. Neurosci."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Cordero, E., Latka, I., Matth\u00e4us, C., Schie, I., and Popp, J. (2018). In-vivo Raman spectroscopy: From basics to applications. J. Biomed. Opt., 23.","DOI":"10.1117\/1.JBO.23.7.071210"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Hanlon, E.B., Manoharan, R., Koo, T.W., Shafer, K.E., Motz, J.T., Fitzmaurice, M., Kramer, J.R., Itzkan, I., Dasari, R.R., and Feld, M.S. (2000). Prospects for in vivo Raman spectroscopy. Phys. Med. Biol., 45.","DOI":"10.1088\/0031-9155\/45\/2\/201"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2396","DOI":"10.1039\/C6AN00041J","article-title":"Optimization of Raman-spectrum baseline correction in biological application","volume":"141","author":"Guo","year":"2016","journal-title":"Analyst"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Birech, Z., Mwangi, P.W., Bukachi, F., and Mandela, K.M. (2017). Application of Raman spectroscopy in type 2 diabetes screening in blood using leucine and isoleucine amino-acids as biomarkers and in comparative anti-diabetic drugs efficacy studies. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0185130"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"18464","DOI":"10.1038\/s41598-022-22197-x","article-title":"Fused Raman spectroscopic analysis of blood and saliva delivers high accuracy for head and neck cancer diagnostics","volume":"12","author":"Koster","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Ryzhikova, E., Ralbovsky, N.M., Sikirzhytski, V., Kazakov, O., Halamkova, L., Quinn, J., Zimmerman, E.A., and Lednev, I.K. (2021). Raman spectroscopy and machine learning for biomedical applications: Alzheimer\u2019s disease diagnosis based on the analysis of cerebrospinal fluid. Spectrochim. Acta A Mol. Biomol. Spectrosc., 248.","DOI":"10.1016\/j.saa.2020.119188"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Sathyavathi, R., Dingari, N.C., Barman, I., Prasad, P.S.R., Prabhakar, S., Narayana Rao, D., Dasari, R.R., and Undamatla, J. (2013). Raman spectroscopy provides a powerful, rapid diagnostic tool for the detection of tuberculous meningitis in ex vivo cerebrospinal fluid samples. J. Biophotonics, 6.","DOI":"10.1002\/jbio.201200110"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"164043","DOI":"10.1016\/j.ijleo.2019.164043","article-title":"Urine Raman spectroscopy for rapid and inexpensive diagnosis of chronic renal failure (CRF) using multiple classification algorithms","volume":"203","author":"Chen","year":"2020","journal-title":"Optik"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e14879","DOI":"10.7717\/peerj.14879","article-title":"Analysis of urine Raman spectra differences from patients with diabetes mellitus and renal pathologies","volume":"11","author":"Kavuru","year":"2023","journal-title":"PeerJ"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Buchan, E., Hardy, M., de Carvalho Gomes, P., Kelleher, L., Chu, H.O.M., and Oppenheimer, P.G. (2022). Emerging Raman spectroscopy and saliva-based diagnostics: From challenges to applications. Appl. Spectrosc. Rev.","DOI":"10.1080\/05704928.2022.2130351"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7897","DOI":"10.1021\/acsami.9b19421","article-title":"Label-Free Surface-Enhanced Raman Spectroscopy Biosensor for On-Site Breast Cancer Detection Using Human Tears","volume":"12","author":"Kim","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Camerlingo, C., Lisitskiy, M., Lepore, M., Portaccio, M., Montorio, D., Del Prete, S., and Cennamo, G. (2019). Characterization of human tear fluid by means of surface-enhanced raman spectroscopy. Sensors, 19.","DOI":"10.3390\/s19051177"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Cennamo, G., Montorio, D., Morra, V.B., Criscuolo, C., Lanzillo, R., Salvatore, E., Camerlingo, C., Lisitskiy, M., Delfino, I., and Portaccio, M. (2020). Surface-enhanced Raman spectroscopy of tears: Toward a diagnostic tool for neurodegenerative disease identification. J. Biomed. Opt., 25.","DOI":"10.1117\/1.JBO.25.8.087002"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1038\/s41551-019-0510-4","article-title":"Rapid optofluidic detection of biomarkers for traumatic brain injury via surface-enhanced Raman spectroscopy","volume":"4","author":"Rickard","year":"2020","journal-title":"Nat. Biomed. Eng."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/s41551-020-0574-1","article-title":"Rapid detection of traumatic brain injury","volume":"4","author":"Li","year":"2020","journal-title":"Nat. Biomed. Eng."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1021\/acsbiomaterials.0c01156","article-title":"Development and Characterization of a Probe Device toward Intracranial Spectroscopy of Traumatic Brain Injury","volume":"7","author":"Mowbray","year":"2021","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Stevens, A.R., Stickland, C.A., Harris, G., Ahmed, Z., Oppenheimer, P.G., Belli, A., and Davies, D.J. (2022). Raman Spectroscopy as a Neuromonitoring Tool in Traumatic Brain Injury: A Systematic Review and Clinical Perspectives. Cells, 11.","DOI":"10.3390\/cells11071227"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1001\/jamaneurol.2020.5079","article-title":"Association of Pharmacological Interventions With Symptom Burden Reduction in Patients With Mild Traumatic Brain Injury: A Systematic Review","volume":"78","author":"Feinberg","year":"2021","journal-title":"JAMA Neurol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1002\/jbio.201300101","article-title":"Dynamic molecular monitoring of retina inflammation by in vivo Raman spectroscopy coupled with multivariate analysis","volume":"7","author":"Marro","year":"2014","journal-title":"J. Biophotonics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1109\/RBME.2022.3161352","article-title":"Review: Emerging Eye-Based Diagnostic Technologies for Traumatic Brain Injury","volume":"16","author":"Harris","year":"2023","journal-title":"IEEE Rev. Biomed. Eng."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1227\/NEU.0b013e318214a81f","article-title":"Neuron-specific enolase, S100B, and glial fibrillary acidic protein levels as outcome predictors in patients with severe traumatic brain injury","volume":"68","author":"Oses","year":"2011","journal-title":"Neurosurgery"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2091","DOI":"10.1007\/s10143-020-01421-0","article-title":"Biomarkers for traumatic brain injury: A short review","volume":"44","author":"Gutierre","year":"2021","journal-title":"Neurosurg. Rev."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/nrneurol.2013.9","article-title":"Biomarkers of mild traumatic brain injury in cerebrospinal fluid and blood","volume":"9","author":"Zetterberg","year":"2013","journal-title":"Nat. Rev. Neurol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.clinbiochem.2020.08.001","article-title":"Interest of blood biomarkers to predict lesions in medical imaging in the context of mild traumatic brain injury","volume":"85","author":"Damien","year":"2020","journal-title":"Clin. Biochem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1021\/cn300216h","article-title":"Gangliosides and CeramidesChange in a Mouse Model of Blast Induced Traumatic Brain Injury","volume":"4","author":"Woods","year":"2013","journal-title":"ACS Chem. Neurosci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e97105","DOI":"10.1172\/jci.insight.97105","article-title":"The current state of biomarkers of mild traumatic brain injury","volume":"3","author":"Kim","year":"2018","journal-title":"JCI Insight"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3114","DOI":"10.1172\/JCI92300","article-title":"Enhanced astrocytic d-serine underlies synaptic damage after traumatic brain injury","volume":"127","author":"Perez","year":"2017","journal-title":"J. Clin. Investig."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Lee, S.H., Kho, A.R., Lee, S.H., Hong, D.K., Kang, B.S., Park, M.K., Lee, C.J., Yang, H.W., Woo, S.Y., and Park, S.W. (2022). Acid Sphingomyelinase Inhibitor, Imipramine, Reduces Hippocampal Neuronal Death after Traumatic Brain Injury. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232314749"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1111\/jnc.13840","article-title":"Imaging mass spectrometry reveals loss of polyunsaturated cardiolipins in the cortical contusion, hippocampus, and thalamus after traumatic brain injury","volume":"139","author":"Sparvero","year":"2016","journal-title":"J. Neurochem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e97677","DOI":"10.1172\/jci.insight.97677","article-title":"Disentangling oxidation\/hydrolysis reactions of brain mitochondrial cardiolipins in pathogenesis of traumatic injury","volume":"3","author":"Chao","year":"2018","journal-title":"JCI Insight"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"6249","DOI":"10.1364\/BOE.399473","article-title":"Spectroscopic detection of traumatic brain injury severity and biochemistry from the retina","volume":"11","author":"Banbury","year":"2020","journal-title":"Biomed. Opt. Express"},{"key":"ref_51","first-page":"68","article-title":"Interleukin-6 Plasmatic Levels in Patients with Head Trauma and Intracerebral Hemorrhage","volume":"5","author":"Antunes","year":"2010","journal-title":"Asian J. Neurosurg."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.bbi.2014.12.021","article-title":"Acute CSF interleukin-6 trajectories after TBI: Associations with neuroinflammation, polytrauma, and outcome","volume":"45","author":"Kumar","year":"2015","journal-title":"Brain. Behav. Immun."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e622","DOI":"10.1002\/ams2.622","article-title":"Thorough overview of ubiquitin C-terminal hydrolase-L1 and glial fibrillary acidic protein as tandem biomarkers recently cleared by US Food and Drug Administration for the evaluation of intracranial injuries among patients with traumatic brain injury","volume":"8","author":"Wang","year":"2021","journal-title":"Acute Med. Surg."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5937","DOI":"10.1016\/j.biomaterials.2013.04.047","article-title":"Transplantation of human mesenchymal stem cells loaded on collagen scaffolds for the treatment of traumatic brain injury in rats","volume":"34","author":"Guan","year":"2013","journal-title":"Biomaterials"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1016\/j.saa.2004.11.017","article-title":"Near infrared Raman spectra of human brain lipids","volume":"61","author":"Krafft","year":"2005","journal-title":"Spectrochim. Acta\u2014Part A Mol. Biomol. Spectrosc."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"71052","DOI":"10.18632\/oncotarget.11958","article-title":"Review: Traumatic brain injury and hyperglycemia, a potentially modifiable risk factor","volume":"7","author":"Shi","year":"2016","journal-title":"Oncotarget"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1212\/WNL.0000000000000105","article-title":"Myoinositol and glutamate complex neurometabolite abnormality after mild traumatic brain injury","volume":"82","author":"Kierans","year":"2014","journal-title":"Neurology"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1159\/000354764","article-title":"Increased Levels of Serum IL-18 Are Associated with the Long-Term Outcome of Severe Traumatic Brain Injury","volume":"21","author":"Ciaramella","year":"2014","journal-title":"Neuroimmunomodulation"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1111\/j.1471-4159.2005.03602.x","article-title":"Extracellular N-acetylaspartate depletion in traumatic brain injury","volume":"96","author":"Belli","year":"2006","journal-title":"J. Neurochem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"41269","DOI":"10.1038\/srep41269","article-title":"Restoring GM1 ganglioside expression ameliorates axonal outgrowth inhibition and cognitive impairments induced by blast traumatic brain injury","volume":"7","author":"Rubovitch","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.4103\/1673-5374.257520","article-title":"Glutathione precursors shield the brain from trauma","volume":"14","author":"Koza","year":"2019","journal-title":"Neural Regen. Res."},{"key":"ref_62","unstructured":"Smith, R., Chepisheva, M., Cronin, T., and Seemungal, B.M. (2019). Neurosensory Disorders in Mild Traumatic Brain Injury, Academic Press."},{"key":"ref_63","first-page":"86","article-title":"Current Trends in Biomarkers for Traumatic Brain Injury","volume":"12","author":"Mehta","year":"2020","journal-title":"Open Access J. Neurol. Neurosurg."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1089\/neu.2007.0444","article-title":"Cortical Injury Increases Cholesterol 24S Hydroxylase (Cyp46) Levels in the Rat Brain","volume":"25","author":"Cartagena","year":"2008","journal-title":"J. Neurotrauma"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Bergholt, M.S., Serio, A., and Albro, M.B. (2019). Raman Spectroscopy: Guiding Light for the Extracellular Matrix. Front. Bioeng. Biotechnol., 7.","DOI":"10.3389\/fbioe.2019.00303"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Chau, A.H., Motz, J.T., Gardecki, J.A., Waxman, S., Bouma, B.E., and Tearney, G.J. (2008). Fingerprint and high-wavenumber Raman spectroscopy in a human-swine coronary xenograft in vivo. J. Biomed. Opt., 13.","DOI":"10.1117\/1.2960015"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"69505","DOI":"10.3389\/fnene.2013.00011","article-title":"N-Acetylaspartate reductions in brain injury: Impact on post-injury neuroenergetics, lipid synthesis, and protein acetylation","volume":"5","author":"Moffett","year":"2013","journal-title":"Front. Neuroenergetics"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1089\/neu.2015.3950","article-title":"Extracellular N-Acetylaspartate in Human Traumatic Brain Injury","volume":"33","author":"Shannon","year":"2016","journal-title":"J. Neurotrauma"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.3389\/fnins.2020.572965","article-title":"Gangliosides in the Brain: Physiology, Pathophysiology and Therapeutic Applications","volume":"14","author":"Sipione","year":"2020","journal-title":"Front. Neurosci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"689","DOI":"10.2174\/1871527317666180627120501","article-title":"Oxidative Stress: Major Threat in Traumatic Brain Injury","volume":"17","author":"Khatri","year":"2018","journal-title":"CNS Neurol. Disord. Drug Targets"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1007\/s11011-016-9801-6","article-title":"Neuron specific enolase: A promising therapeutic target in acute spinal cord injury","volume":"31","author":"Haque","year":"2016","journal-title":"Metab. Brain Dis."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.tins.2015.04.003","article-title":"Glial fibrillary acidic protein: From intermediate filament assembly and gliosis to neurobiomarker","volume":"38","author":"Yang","year":"2015","journal-title":"Trends Neurosci."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.tins.2009.08.002","article-title":"Molecular dissection of reactive astrogliosis and glial scar formation","volume":"32","author":"Sofroniew","year":"2009","journal-title":"Trends Neurosci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"335","DOI":"10.2174\/1568010054022051","article-title":"Prostaglandins and cyclooxygenases in glial cells during brain inflammation","volume":"4","author":"Tzeng","year":"2005","journal-title":"Curr. Drug Targets Inflamm. Allergy"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"4643","DOI":"10.1073\/pnas.1821282116","article-title":"Role of UCHL1 in axonal injury and functional recovery after cerebral ischemia","volume":"116","author":"Liu","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/02699052.2017.1382717","article-title":"The diagnostic values of UCH-L1 in traumatic brain injury: A meta-analysis","volume":"32","author":"Shahjouei","year":"2017","journal-title":"Brain Inj."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1136\/mp.56.3.132","article-title":"Genetic vulnerability following traumatic brain injury: The role of apolipoprotein E","volume":"56","author":"Nathoo","year":"2003","journal-title":"Mol. Pathol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1039\/C6AN02238C","article-title":"Label-free monitoring of tissue biochemistry following traumatic brain injury using Raman spectroscopy","volume":"142","author":"Surmacki","year":"2017","journal-title":"Analyst"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1089\/089771503767869953","article-title":"Remodeling of Cerebrospinal Fluid Lipoprotein Particles after Human Traumatic Brain Injury","volume":"20","author":"Kay","year":"2004","journal-title":"J. Neurotrauma"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"e13126","DOI":"10.1111\/bpa.13126","article-title":"Brain-specific loss of Abcg1 disturbs cholesterol metabolism and aggravates pyroptosis and neurological deficits after traumatic brain injury","volume":"33","author":"Xu","year":"2023","journal-title":"Brain Pathol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1002\/glia.24161","article-title":"Inhibition of glial D-serine release rescues synaptic damage after brain injury","volume":"70","author":"Tapanes","year":"2022","journal-title":"Glia"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"513554","DOI":"10.3389\/fneur.2020.00437","article-title":"Exploring Sphingolipid Implications in Neurodegeneration","volume":"11","author":"Alessenko","year":"2020","journal-title":"Front. Neurol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"e0421-18","DOI":"10.1523\/ENEURO.0421-18.2019","article-title":"Sphingomyelin in Brain and Cognitive Development: Preliminary Data","volume":"6","author":"Schneider","year":"2019","journal-title":"eNeuro"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"416109","DOI":"10.3389\/fnins.2019.00103","article-title":"Converging and differential brain phospholipid dysregulation in the pathogenesis of repetitive mild traumatic brain injury and Alzheimer\u2019s disease","volume":"13","author":"Ojo","year":"2019","journal-title":"Front. Neurosci."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jbior.2018.09.013","article-title":"Sphingolipids in neurodegeneration (with focus on ceramide and S1P)","volume":"70","author":"Wang","year":"2018","journal-title":"Adv. Biol. Regul."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1089\/neu.1993.10.287","article-title":"Alterations of protein kinase C in rat hippocampus following traumatic brain injury","volume":"10","author":"Yang","year":"1993","journal-title":"J. Neurotrauma"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1194\/jlr.R026682","article-title":"Role of sulfatide in normal and pathological cells and tissues","volume":"53","author":"Takahashi","year":"2012","journal-title":"J. Lipid Res."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Paradies, G., Paradies, V., Ruggiero, F.M., and Petrosillo, G. (2019). Role of Cardiolipin in Mitochondrial Function and Dynamics in Health and Disease: Molecular and Pharmacological Aspects. Cells, 8.","DOI":"10.3390\/cells8070728"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1038\/ncb2837","article-title":"Cardiolipin externalization to the outer mitochondrial membrane acts as an elimination signal for mitophagy in neuronal cells","volume":"15","author":"Chu","year":"2013","journal-title":"Nat. Cell Biol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1930","DOI":"10.1523\/JNEUROSCI.3415-17.2018","article-title":"Cardiolipin-Dependent Mitophagy Guides Outcome after Traumatic Brain Injury","volume":"39","author":"Chao","year":"2019","journal-title":"J. Neurosci."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/1742-2094-4-17","article-title":"Acute and delayed neuroinflammatory response following experimental penetrating ballistic brain injury in the rat","volume":"4","author":"Williams","year":"2007","journal-title":"J. Neuroinflamm."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"3035","DOI":"10.1007\/s10143-022-01827-y","article-title":"Interleukin-6 as a prognostic biomarker of clinical outcomes after traumatic brain injury: A systematic review","volume":"45","author":"Ooi","year":"2022","journal-title":"Neurosurg. Rev."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"ii43","DOI":"10.1093\/rheumatology\/kex513","article-title":"Physiological effects of modulating the interleukin-6 axis","volume":"57","author":"Schett","year":"2018","journal-title":"Rheumatology"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"4037","DOI":"10.1073\/pnas.052020999","article-title":"Glial protein S100B modulates long-term neuronal synaptic plasticity","volume":"99","author":"Nishiyama","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1089\/neu.2012.2579","article-title":"S100b as a Prognostic Biomarker in Outcome Prediction for Patients with Severe Traumatic Brain Injury","volume":"30","author":"Goyal","year":"2013","journal-title":"J. Neurotrauma"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/nrneurol.2013.163","article-title":"Lysosomal storage diseases\u2014The horizon expands","volume":"9","author":"Boustany","year":"2013","journal-title":"Nat. Rev. Neurol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/S0304-4165(02)00410-5","article-title":"Galactolipids are molecular determinants of myelin development and axo\u2013glial organization","volume":"1573","author":"Marcus","year":"2002","journal-title":"Biochim. Biophys. Acta\u2014Gen. Subj."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.tins.2013.07.001","article-title":"Sugar for the brain: The role of glucose in physiological and pathological brain function","volume":"36","author":"Mergenthaler","year":"2013","journal-title":"Trends Neurosci."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1097\/01.WCB.0000076701.45782.EF","article-title":"Persistently low extracellular glucose correlates with poor outcome 6 months after human traumatic brain injury despite a lack of increased lactate: A microdialysis study","volume":"23","author":"Vespa","year":"2003","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1007\/s11011-014-9628-y","article-title":"Glucose metabolism following human traumatic brain injury: Methods of assessment and pathophysiological findings","volume":"30","author":"Jalloh","year":"2015","journal-title":"Metab. Brain Dis."},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"L\u00f3pez-Gambero, A.J., Sanjuan, C., Serrano-Castro, P.J., Su\u00e1rez, J., and Fonseca, F.R. (2020). De The Biomedical Uses of Inositols: A Nutraceutical Approach to Metabolic Dysfunction in Aging and Neurodegenerative Diseases. Biomedicines, 8.","DOI":"10.3390\/biomedicines8090295"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1159\/000111347","article-title":"Multinuclear NMR studies on the energy metabolism of glial and neuronal cells","volume":"15","author":"Brand","year":"1993","journal-title":"Dev. Neurosci."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"2079","DOI":"10.1016\/j.neuroimage.2010.10.017","article-title":"In vivo mapping of brain myo-inositol","volume":"54","author":"Haris","year":"2011","journal-title":"Neuroimage"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1203\/01.PDR.0000139928.60530.7D","article-title":"Proton Spectroscopy Detected Myoinositol in Children with Traumatic Brain Injury","volume":"56","author":"Ashwal","year":"2004","journal-title":"Pediatr. Res."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1742-2094-7-9","article-title":"Interleukin 18 in the CNS","volume":"7","author":"Alboni","year":"2010","journal-title":"J. Neuroinflamm."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.tins.2005.06.008","article-title":"IL-18: A key player in neuroinflammation and neurodegeneration?","volume":"28","author":"Schmidt","year":"2005","journal-title":"Trends Neurosci."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"46","DOI":"10.12703\/r\/10-46","article-title":"Neurofilament light: A narrative review on biomarker utility","volume":"10","author":"Narayanan","year":"2021","journal-title":"Fac. Rev."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/s41582-018-0058-z","article-title":"Neurofilaments as biomarkers in neurological disorders","volume":"14","author":"Khalil","year":"2018","journal-title":"Nat. Rev. Neurol."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1136\/jnnp-2018-320106","article-title":"Neurofilament light chain as a biomarker in neurological disorders","volume":"90","author":"Gaetani","year":"2019","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1016\/S1474-4422(17)30371-X","article-title":"Traumatic brain injury: Integrated approaches to improve prevention, clinical care, and research","volume":"16","author":"Maas","year":"2017","journal-title":"Lancet Neurol."},{"key":"ref_111","unstructured":"Zhang, J., Puvenna, V., and Janigro, D. (2016). Translational Research in Traumatic Brain Injury, CRC Press\/Taylor and Francis Group."},{"key":"ref_112","unstructured":"Socrates, G. (2004). Infrared and Raman Characteristic Group Frequencies: Tables and Charts, John Wiley & Sons, Ltd.. [3rd ed.]."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.saa.2015.03.086","article-title":"Complementary analysis of tissue homogenates composition obtained by Vis and NIR laser excitations and Raman spectroscopy","volume":"147","author":"Malek","year":"2015","journal-title":"Spectrochim. Acta\u2014Part A Mol. Biomol. Spectrosc."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"14368","DOI":"10.1039\/D0RA01173H","article-title":"Raman profile alterations of irradiated human nasopharyngeal cancer cells detected with laser tweezer Raman spectroscopy","volume":"10","author":"Qiu","year":"2020","journal-title":"RSC Adv."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0009-8981(03)00200-6","article-title":"Analysis of glutathione: Implication in redox and detoxification","volume":"333","author":"Pastore","year":"2003","journal-title":"Clin. Chim. Acta"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"8188","DOI":"10.1021\/acsomega.9b00629","article-title":"Raman-Based in Situ Monitoring of Changes in Molecular Signatures during Mitochondrially Mediated Apoptosis","volume":"4","author":"Shin","year":"2019","journal-title":"ACS Omega"},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1007\/s11084-015-9477-7","article-title":"Defining Multiple Characteristic Raman Bands of \u03b1-Amino Acids as Biomarkers for Planetary Missions Using a Statistical Method","volume":"46","author":"Rolfe","year":"2016","journal-title":"Orig. Life Evol. Biosph."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.3389\/fonc.2019.01165","article-title":"Rapid Label-Free Analysis of Brain Tumor Biopsies by Near Infrared Raman and Fluorescence Spectroscopy\u2014A Study of 209 Patients","volume":"9","author":"Galli","year":"2019","journal-title":"Front. Oncol."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1002\/jrs.6204","article-title":"Raman spectroscopy in cell biology and microbiology","volume":"52","author":"Pezzotti","year":"2021","journal-title":"J. Raman Spectrosc."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1002\/ijc.11500","article-title":"Near-infrared Raman spectroscopy for optical diagnosis of lung cancer","volume":"107","author":"Huang","year":"2003","journal-title":"Int. J. Cancer"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.vibspec.2007.04.002","article-title":"Raman spectroscopy of glycerol\/D2O solutions","volume":"45","author":"Mudalige","year":"2007","journal-title":"Vib. Spectrosc."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1186\/s13054-016-1450-y","article-title":"Utility of neuron-specific enolase in traumatic brain injury; relations to S100B levels, outcome, and extracranial injury severity","volume":"20","author":"Thelin","year":"2016","journal-title":"Crit. Care"},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1080\/05704920701551530","article-title":"Raman Spectroscopy of Biological Tissues","volume":"42","author":"Movasaghi","year":"2007","journal-title":"Appl. Spectrosc. Rev."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"3673","DOI":"10.1039\/C2AN16292J","article-title":"Confocal Raman microspectroscopy for skin characterization: A comparative study between human skin and pig skin","volume":"137","author":"Tfaili","year":"2012","journal-title":"Analyst"},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"7971","DOI":"10.1038\/s41598-019-44353-6","article-title":"Low frequency Raman Spectroscopy for micron-scale and in vivo characterization of elemental sulfur in microbial samples","volume":"9","author":"Nims","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1093\/mmy\/myx078","article-title":"Nail Raman spectroscopy: A promising method for the diagnosis of onychomycosis. An ex vivo pilot study","volume":"56","author":"Kourkoumelis","year":"2018","journal-title":"Med. Mycol."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/0022-2860(82)85050-3","article-title":"Surface enhanced raman spectroscopy of nucleic acid bases on Ag electrodes","volume":"79","author":"Koglin","year":"1982","journal-title":"J. Mol. Struct."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"8015024","DOI":"10.1155\/2020\/8015024","article-title":"Identification of lesional tissues and nonlesional tissues in early gastric cancer endoscopic submucosal dissection specimens using a fiber optic raman system","volume":"2020","author":"Luan","year":"2020","journal-title":"Gastroenterol. Res. Pract."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"9079","DOI":"10.1038\/s41598-021-88640-7","article-title":"Spectroscopic evaluation of carcinogenesis in endometrial cancer","volume":"11","author":"Depciuch","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"3700","DOI":"10.1021\/acsomega.8b03675","article-title":"Complementarity of Raman and Infrared Spectroscopy for Structural Characterization of Plant Epicuticular Waxes","volume":"4","author":"Farber","year":"2019","journal-title":"ACS Omega"},{"key":"ref_131","doi-asserted-by":"crossref","unstructured":"Zhou, Y., Liu, C., Sun, Y., Pu, Y., Boydston-White, S., Liu, Y., and Alfano, R.R. (2012). Human brain cancer studied by resonance Raman spectroscopy. J. Biomed. Opt., 17.","DOI":"10.1117\/1.JBO.17.11.116021"},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/j.saa.2017.07.046","article-title":"Epileptic rat brain tissue analyzed by 2D correlation Raman spectroscopy","volume":"188","author":"Sacharz","year":"2018","journal-title":"Spectrochim. Acta\u2014Part A Mol. Biomol. Spectrosc."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1752-153X-7-37","article-title":"Surface-enhanced Raman spectroscopy investigation on human breast cancer cells","volume":"7","author":"Zhu","year":"2013","journal-title":"Chem. Cent. J."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"2527","DOI":"10.1007\/s43032-021-00494-6","article-title":"Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy","volume":"28","author":"Jahmani","year":"2021","journal-title":"Reprod. Sci."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1002\/jrs.4581","article-title":"Raman spectroscopy at different excitation wavelengths (1064, 785 and 532\u2009nm) as a tool for diagnosis of colon cancer","volume":"45","author":"Synytsya","year":"2014","journal-title":"J. Raman Spectrosc."},{"key":"ref_136","doi-asserted-by":"crossref","unstructured":"Katsara, K., Psatha, K., Kenanakis, G., Aivaliotis, M., and Papadakis, V.M. (2022). Subtyping on Live Lymphoma Cell Lines by Raman Spectroscopy. Materials, 15.","DOI":"10.3390\/ma15020546"},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1039\/c0an00897d","article-title":"Detection of acute brain injury by Raman spectral signature","volume":"136","author":"Tay","year":"2011","journal-title":"Analyst"},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1021\/acs.chemrev.0c00487","article-title":"Power of Infrared and Raman Spectroscopies to Characterize Metal-Organic Frameworks and Investigate Their Interaction with Guest Molecules","volume":"121","author":"Hadjiivanov","year":"2021","journal-title":"Chem. Rev."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.molliq.2015.05.015","article-title":"Intermolecular interactions in mixtures of 1-n-butyl-3-methylimidazolium acetate and water: Insights from IR, Raman, NMR spectroscopy and quantum chemistry calculations","volume":"210","author":"Marekha","year":"2015","journal-title":"J. Mol. Liq."},{"key":"ref_140","first-page":"171","article-title":"Raman Spectroscopy of Amino Acid Crystals","volume":"201","author":"Freire","year":"2017","journal-title":"Raman Spectrosc. Appl."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1097\/TA.0b013e3182491e3d","article-title":"Serum levels of ubiquitin C-terminal hydrolase distinguish mild traumatic brain injury from trauma controls and are elevated in mild and moderate traumatic brain injury patients with intracranial lesions and neurosurgical intervention","volume":"72","author":"Papa","year":"2012","journal-title":"J. Trauma Acute Care Surg."},{"key":"ref_142","doi-asserted-by":"crossref","unstructured":"Bik, E., Dorosz, A., Mateuszuk, L., Baranska, M., and Majzner, K. (2020). Fixed versus live endothelial cells: The effect of glutaraldehyde fixation manifested by characteristic bands on the Raman spectra of cells. Spectrochim. Acta Part A Mol. Biomol. Spectrosc., 240.","DOI":"10.1016\/j.saa.2020.118460"},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"103073","DOI":"10.1016\/j.vibspec.2020.103073","article-title":"Raman spectroscopy characterization of the major classes of plasma lipoproteins","volume":"109","author":"Ricciardi","year":"2020","journal-title":"Vib. Spectrosc."},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1002\/jrs.4607","article-title":"Raman spectroscopy of lipids: A review","volume":"46","author":"Czamara","year":"2015","journal-title":"J. Raman Spectrosc."},{"key":"ref_145","unstructured":"Huff, T., Boyd, B., and Jialal, I. (2017). StatPearls, StatPearls Publishing."},{"key":"ref_146","doi-asserted-by":"crossref","unstructured":"Ito, T., Hamauchi, N., Hagi, T., Morohashi, N., Hemmi, H., Sato, Y.G., Saito, T., and Yoshimura, T. (2018). D-serine metabolism and its importance in development of Dictyostelium discoideum. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.00784"},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.plipres.2013.05.001","article-title":"Biological functions of sphingomyelins","volume":"52","author":"Slotte","year":"2013","journal-title":"Prog. Lipid Res."},{"key":"ref_148","doi-asserted-by":"crossref","unstructured":"Kurek, K., \u0141ukaszuk, B., Piotrowska, D.M., Wiesio\u0142ek, P., Chabowska, A.M., and Zendzian-Piotrowska, M. (2013). Metabolism, physiological role, and clinical implications of sphingolipids in gastrointestinal tract. BioMed Res. Int., 2013.","DOI":"10.1155\/2013\/908907"},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"13142","DOI":"10.1074\/jbc.M113.530311","article-title":"Essential roles of neutral ceramidase and sphingosine in mitochondrial dysfunction due to traumatic brain injury","volume":"289","author":"Novgorodov","year":"2014","journal-title":"J. Biol. Chem."},{"key":"ref_150","first-page":"5","article-title":"The Raman study on certain sulfates","volume":"55","author":"Buzgar","year":"2009","journal-title":"Analele Stiintifice Univ. Al. I. Cuza"},{"key":"ref_151","unstructured":"Uphoff, A., Hermansson, M., Haimi, P., and Somerharju, P. (2008). Medical Applications of Mass Spectrometry, Elsevier."},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1007\/s002230001206","article-title":"Raman spectroscopic detection of changes in bioapatite in mouse femora as a function of age and in vitro fluoride treatment","volume":"68","author":"Freeman","year":"2001","journal-title":"Calcif. Tissue Int."},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1093\/aob\/mcl059","article-title":"A Raman-scattering Study on the Net Orientation of Biomacromolecules in the Outer Epidermal Walls of Mature Wheat Stems (Triticum aestivum)","volume":"97","author":"Cao","year":"2006","journal-title":"Ann. Bot."},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1007\/s00425-019-03216-0","article-title":"Raman spectroscopy as an early detection tool for rose rosette infection","volume":"250","author":"Farber","year":"2019","journal-title":"Planta"},{"key":"ref_155","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.phpro.2015.09.161","article-title":"Detailed Analysis of the Structural Changes of Bone Matrix during the Demineralization Process Using Raman Spectroscopy","volume":"73","author":"Timchenko","year":"2015","journal-title":"Phys. Procedia"},{"key":"ref_156","doi-asserted-by":"crossref","unstructured":"Calcagnile, O., Anell, A., and Und\u00e9n, J. (2016). The addition of S100B to guidelines for management of mild head injury is potentially cost saving. BMC Neurol., 16.","DOI":"10.1186\/s12883-016-0723-z"},{"key":"ref_157","doi-asserted-by":"crossref","unstructured":"Li, Y., Pan, J., Chen, G., Li, C., Lin, S., Shao, Y., Feng, S., Huang, Z., Xie, S., and Zeng, H. (2013). Micro-Raman spectroscopy study of cancerous and normal nasopharyngeal tissues. J. Biomed. Opt., 18.","DOI":"10.1117\/1.JBO.18.2.027003"},{"key":"ref_158","doi-asserted-by":"crossref","unstructured":"Girard, A., Cooper, A., Mabbott, S., Bradley, B., Asiala, S., Jamieson, L., Clucas, C., Capewell, P., Marchesi, F., and Gibbins, M.P. (2021). Raman spectroscopic analysis of skin as a diagnostic tool for Human African Trypanosomiasis. PLoS Pathog., 17.","DOI":"10.1371\/journal.ppat.1010060"},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.jaci.2005.03.023","article-title":"Antibody responses against galactocerebroside are potential stage-specific biomarkers in multiple sclerosis","volume":"116","author":"Menge","year":"2005","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_160","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2217\/clp.11.8","article-title":"The structural and functional role of myelin fast-migrating cerebrosides: Pathological importance in multiple sclerosis","volume":"6","author":"Podbielska","year":"2011","journal-title":"Clin. Lipidol."},{"key":"ref_161","doi-asserted-by":"crossref","first-page":"2545","DOI":"10.1038\/s41467-022-30227-5","article-title":"Serum metabolome associated with severity of acute traumatic brain injury","volume":"13","author":"Thomas","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_162","doi-asserted-by":"crossref","unstructured":"Ayala, O.D., Doster, R.S., Manning, S.D., O\u2019Brien, C.M., Aronoff, D.M., Gaddy, J.A., and Mahadevan-Jansen, A. (2019). Raman microspectroscopy differentiates perinatal pathogens on ex vivo infected human fetal membrane tissues. J. Biophotonics, 12.","DOI":"10.1002\/jbio.201800449"},{"key":"ref_163","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1021\/bi00383a021","article-title":"Assignment of Fingerprint Vibrations in the Resonance Raman Spectra of Rhodopsin, Isorhodopsin, and Bathorhodopsin: Implications for Chromophore Structure and Environment","volume":"26","author":"Palings","year":"1987","journal-title":"Biochemistry"},{"key":"ref_164","doi-asserted-by":"crossref","first-page":"832522","DOI":"10.3389\/fpls.2022.832522","article-title":"Raman Spectroscopy Detects Changes in Carotenoids on the Surface of Watermelon Fruits during Maturation","volume":"13","author":"Dhanani","year":"2022","journal-title":"Front. Plant Sci."},{"key":"ref_165","doi-asserted-by":"crossref","first-page":"012025","DOI":"10.1088\/1742-6596\/277\/1\/012025","article-title":"Chloride ion-dependent surface-enhanced Raman scattering study of biotin on the silver surface","volume":"277","author":"Liu","year":"2011","journal-title":"J. Phys. Conf. Ser."},{"key":"ref_166","doi-asserted-by":"crossref","first-page":"11510","DOI":"10.3390\/s111211510","article-title":"Contrastive Analysis of the Raman Spectra of Polychlorinated Benzene: Hexachlorobenzene and Benzene","volume":"11","author":"Zhang","year":"2011","journal-title":"Sensors"},{"key":"ref_167","doi-asserted-by":"crossref","unstructured":"Khnykina, K.A., Kireev, V.V., Krunina, N.V., Kundikova, N.D., and Verina, E.V. (2018, January 13\u201315). Influence of Environment on Raman Spectra of Glycine and Prospect of Their Use for Functional Diagnostics of Human Beings. Proceedings of the 2018 Global Smart Industry Conference (GloSIC), Chelyabinsk, Russia.","DOI":"10.1109\/GloSIC.2018.8570138"},{"key":"ref_168","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0924-2031(98)00004-6","article-title":"Infrared and Raman spectra of alkaline-earth and copper(II) acetates in aqueous solutions","volume":"16","author":"Burneau","year":"1998","journal-title":"Vib. Spectrosc."},{"key":"ref_169","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.1021\/ja00449a002","article-title":"Raman Spectroscopy of Uncomplexed Valinomycin. 1. The Solid State","volume":"99","author":"Asher","year":"1977","journal-title":"J. Am. Chem. Soc."},{"key":"ref_170","doi-asserted-by":"crossref","first-page":"15048","DOI":"10.1038\/s41598-018-33417-8","article-title":"Raman spectroscopic detection of high-grade cervical cytology: Using morphologically normal appearing cells","volume":"8","author":"Duraipandian","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_171","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1155\/2006\/328210","article-title":"Near Infrared Raman Spectroscopy (NIRS): A technique for doping control","volume":"20","author":"Pacheco","year":"2006","journal-title":"J. Spectrosc."},{"key":"ref_172","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/S0006-3495(90)82604-7","article-title":"Resonance Raman enhancement of phenyl ring vibrational modes in phenyl iron complex of myoglobin","volume":"57","author":"Liu","year":"1990","journal-title":"Biophys. J."},{"key":"ref_173","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1177\/00037028211043119","article-title":"Raman Spectroscopy for the Analysis of Microplastics in Aquatic Systems","volume":"75","author":"Nava","year":"2021","journal-title":"Appl. Spectrosc."},{"key":"ref_174","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1002\/jrs.5588","article-title":"Infrared and Raman spectra of lignin substructures: Coniferyl alcohol, abietin, and coniferyl aldehyde","volume":"50","author":"Bock","year":"2019","journal-title":"J. Raman Spectrosc."},{"key":"ref_175","doi-asserted-by":"crossref","first-page":"101531","DOI":"10.1016\/j.jscs.2022.101531","article-title":"Surface-Enhanced Raman Spectroscopy (SERS) for characterization SARS-CoV-2","volume":"26","author":"Durigo","year":"2022","journal-title":"J. Saudi Chem. Soc."},{"key":"ref_176","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.bjp.2015.09.002","article-title":"Rapid assessment of chemical compounds from Phyllogorgia dilatata using Raman spectroscopy","volume":"25","author":"Maia","year":"2015","journal-title":"Rev. Bras. Farmacogn."},{"key":"ref_177","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.pdpdt.2019.08.006","article-title":"Application of Raman spectroscopy in the detection of hepatitis B virus infection","volume":"28","author":"Tong","year":"2019","journal-title":"Photodiagnosis Photodyn. Ther."},{"key":"ref_178","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1002\/jrs.4335","article-title":"Raman spectroscopy of proteins: A review","volume":"44","author":"Rygula","year":"2013","journal-title":"J. Raman Spectrosc."},{"key":"ref_179","doi-asserted-by":"crossref","unstructured":"Bankapur, A., Zachariah, E., Chidangil, S., Valiathan, M., and Mathur, D. (2010). Raman Tweezers Spectroscopy of Live, Single Red and White Blood Cells. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0010427"},{"key":"ref_180","doi-asserted-by":"crossref","first-page":"9262","DOI":"10.1021\/acs.jpcc.9b01355","article-title":"Raman, SERS, and DFT Analysis of the Main Alkaloids Contained in Syrian Rue","volume":"123","author":"Pozzi","year":"2019","journal-title":"J. Phys. Chem. C"},{"key":"ref_181","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1515\/opag-2020-0210","article-title":"Evaluation of FT-Raman and FTIR-ATR spectroscopy for the quality evaluation of Lavandula spp. Honey","volume":"6","author":"Anjos","year":"2021","journal-title":"Open Agric."},{"key":"ref_182","first-page":"381","article-title":"Evaluation of degradation in chemical compounds of wood in historical buildings using FT-IR and FT-Raman vibrational spectroscopy","volume":"21","author":"Moosavinejad","year":"2019","journal-title":"Maderas. Cienc. Tecnol."},{"key":"ref_183","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1002\/jrs.2726","article-title":"FT-Raman spectroscopy\u2014A rapid and reliable quantification protocol for the determination of natural indigo dye in Polygonum tinctorium","volume":"42","author":"Fiedler","year":"2011","journal-title":"J. Raman Spectrosc."},{"key":"ref_184","doi-asserted-by":"crossref","first-page":"012009","DOI":"10.1088\/1742-6596\/277\/1\/012009","article-title":"The determination of captopril in Solution by Raman spectroscopy","volume":"277","author":"Gao","year":"2011","journal-title":"J. Phys. Conf. Ser."},{"key":"ref_185","doi-asserted-by":"crossref","first-page":"9673","DOI":"10.1039\/C9SC03296G","article-title":"Tip-enhanced Raman spectroscopy for structural analysis of two-dimensional covalent monolayers synthesized on water and on Au (111)","volume":"10","author":"Zheng","year":"2019","journal-title":"Chem. Sci."},{"key":"ref_186","doi-asserted-by":"crossref","first-page":"5340","DOI":"10.1038\/ncomms6340","article-title":"The structural basis for receptor recognition of human interleukin-18","volume":"5","author":"Tsutsumi","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_187","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1366\/000370207779947585","article-title":"Relative Intensity Correction of Raman Spectrometers: NIST SRMs 2241 Through 2243 for 785 nm, 532 nm, and 488 nm","volume":"61","author":"Choquette","year":"2007","journal-title":"Appl. Spectrosc."},{"key":"ref_188","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1186\/s11671-019-3039-2","article-title":"Raman Techniques: Fundamentals and Frontiers","volume":"14","author":"Jones","year":"2019","journal-title":"Nanoscale Res. Lett."},{"key":"ref_189","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1242\/dmm.002733","article-title":"Rats!","volume":"2","author":"Iannaccone","year":"2009","journal-title":"Dis. Model. Mech."},{"key":"ref_190","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1186\/s12967-019-2052-7","article-title":"Modeling trauma in rats: Similarities to humans and potential pitfalls to consider","volume":"17","author":"Weber","year":"2019","journal-title":"J. Transl. Med."},{"key":"ref_191","doi-asserted-by":"crossref","first-page":"4272","DOI":"10.1016\/S0021-9258(18)62081-2","article-title":"Enzymatic Synthesis of Galactocerebroside by a Galactosyltransferase from Embryonic Chicken Brain","volume":"246","author":"Basu","year":"1971","journal-title":"J. Biol. Chem."},{"key":"ref_192","doi-asserted-by":"crossref","first-page":"15893","DOI":"10.1038\/s41598-018-34329-3","article-title":"Plasma lipidomic analysis reveals strong similarities between lipid fingerprints in human, hamster and mouse compared to other animal species","volume":"8","author":"Kaabia","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_193","doi-asserted-by":"crossref","first-page":"6403","DOI":"10.1021\/acsomega.1c07070","article-title":"Analysis and Comparison of Mouse and Human Brain Gangliosides via Two-Stage Matching of MS\/MS Spectra","volume":"7","author":"Huang","year":"2022","journal-title":"ACS Omega"},{"key":"ref_194","doi-asserted-by":"crossref","unstructured":"Jiang, Z., Shen, T., Huynh, H., Fang, X., Han, Z., and Ouyang, K. (2022). Cardiolipin Regulates Mitochondrial Ultrastructure and Function in Mammalian Cells. Genes, 13.","DOI":"10.3390\/genes13101889"},{"key":"ref_195","first-page":"108","article-title":"Alzheimer\u2019s disease biomarkers in animal models: Closing the translational gap","volume":"2","author":"Sabbagh","year":"2013","journal-title":"Am. J. Neurodegener. Dis."},{"key":"ref_196","doi-asserted-by":"crossref","first-page":"10812","DOI":"10.1038\/s41598-019-47205-5","article-title":"Development of the Self Optimising Kohonen Index Network (SKiNET) for Raman Spectroscopy Based Detection of Anatomical Eye Tissue","volume":"9","author":"Banbury","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_197","doi-asserted-by":"crossref","first-page":"115796","DOI":"10.1016\/j.trac.2019.115796","article-title":"Deep learning and artificial intelligence methods for Raman and surface-enhanced Raman scattering","volume":"124","author":"Lussier","year":"2020","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_198","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1093\/jalm.2019.031393","article-title":"Predictive Performance of Traumatic Brain Injury Biomarkers in High-Risk Elderly Patients","volume":"5","author":"Ward","year":"2020","journal-title":"J. Appl. Lab. Med."},{"key":"ref_199","doi-asserted-by":"crossref","unstructured":"Terrones, O., Olazar-Intxausti, J., Anso, I., Lorizate, M., Nieto-Garai, J.A., and Contreras, F.X. (2023). Raman Spectroscopy as a Tool to Study the Pathophysiology of Brain Diseases. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24032384"},{"key":"ref_200","doi-asserted-by":"crossref","first-page":"3622","DOI":"10.1364\/BOE.495285","article-title":"Serum-based Raman spectroscopic diagnosis of blast-induced brain injury in a rat model","volume":"14","author":"Ge","year":"2023","journal-title":"Biomed. Opt. Express"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/22\/2589\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:19:12Z","timestamp":1760131152000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/22\/2589"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,8]]},"references-count":200,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2023,11]]}},"alternative-id":["cells12222589"],"URL":"https:\/\/doi.org\/10.3390\/cells12222589","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,8]]}}}