{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:44:11Z","timestamp":1760150651947,"version":"build-2065373602"},"reference-count":60,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T00:00:00Z","timestamp":1702598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["075-15-2021-596","21-15-00349"],"award-info":[{"award-number":["075-15-2021-596","21-15-00349"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["075-15-2021-596","21-15-00349"],"award-info":[{"award-number":["075-15-2021-596","21-15-00349"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Human Wharton\u2019s jelly mesenchymal stem cells (hWJ-MSCs) are of great interest in tissue engineering. We obtained hWJ-MSCs from four patients, and then we stimulated their chondrogenic phenotype formation in vitro by adding resveratrol (during cell expansion) and a canonical Wnt pathway activator, LiCl, as well as a Rho-associated protein kinase inhibitor, Y27632 (during differentiation). The effects of the added reagents on the formation of hWJ-MSC sheets destined to repair osteochondral injuries were investigated. Three-dimensional hWJ-MSC sheets grown on P(NIPAM-co-NtBA)-based matrices were characterized in vitro and in vivo. The combination of resveratrol and LiCl showed effects on hWJ-MSC sheets similar to those of the basal chondrogenic medium. Adding Y27632 decreased both the proportion of hypertrophied cells and the expression of the hyaline cartilage markers. In vitro, DMSO was observed to impede the effects of the chondrogenic factors. The mouse knee defect model experiment revealed that hWJ-MSC sheets grown with the addition of resveratrol and Y27632 were well integrated with the surrounding tissues; however, after 3 months, the restored tissue was identical to that of the naturally healed cartilage injury. Thus, the combination of chondrogenic supplements may not always have additive effects on the progress of cell culture and could be neutralized by the microenvironment after transplantation.<\/jats:p>","DOI":"10.3390\/cells12242845","type":"journal-article","created":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T06:53:16Z","timestamp":1702623196000},"page":"2845","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Resveratrol\u2019s Impact on the Chondrogenic Reagents\u2019 Effects in Cell Sheet Cultures of Wharton\u2019s Jelly-Derived MSCs"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6905-9703","authenticated-orcid":false,"given":"Anastasiia D.","family":"Kurenkova","sequence":"first","affiliation":[{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktoria S.","family":"Presniakova","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zlata A.","family":"Mosina","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1305-3905","authenticated-orcid":false,"given":"Pavel D.","family":"Kibirskiy","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5224-4611","authenticated-orcid":false,"given":"Irina A.","family":"Romanova","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4283-1980","authenticated-orcid":false,"given":"Gilyana K.","family":"Tugaeva","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nastasia V.","family":"Kosheleva","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia"},{"name":"FSBSI \u201cInstitute of General Pathology and Pathophysiology\u201d, Baltiyskaya St. 8, Moscow 125315, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5062-8280","authenticated-orcid":false,"given":"Kirill S.","family":"Vinogradov","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7466-3662","authenticated-orcid":false,"given":"Sergei V.","family":"Kostjuk","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia"},{"name":"Department of Chemistry, Belarussian State University, 14 Leningradskaya St., 220006 Minsk, Belarus"},{"name":"Research Institute for Physical Chemical Problems of the Belarusian State University, 14 Leningradskaya St., 220006 Minsk, Belarus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana L.","family":"Kotova","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yury A.","family":"Rochev","sequence":"additional","affiliation":[{"name":"Center for Research in Medical Devices (C\u00daRAM), National University of Ireland Galway, H91 W2TY Galway, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina V.","family":"Medvedeva","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter S.","family":"Timashev","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"218","DOI":"10.15283\/ijsc18034","article-title":"Mesenchymal stem cells from the Wharton\u2019s jelly of the human umbilical cord: Biological properties and therapeutic potential","volume":"12","author":"Marino","year":"2019","journal-title":"Int. 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