{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T02:24:25Z","timestamp":1788834265958,"version":"build-2803163510"},"reference-count":44,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,23]],"date-time":"2023-12-23T00:00:00Z","timestamp":1703289600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wellcome Award","award":["207684\/Z\/17\/Z"],"award-info":[{"award-number":["207684\/Z\/17\/Z"]}]},{"name":"Wellcome Award","award":["MR\/S03241X\/1"],"award-info":[{"award-number":["MR\/S03241X\/1"]}]},{"name":"Wellcome Award","award":["BB\/V010344\/1"],"award-info":[{"award-number":["BB\/V010344\/1"]}]},{"DOI":"10.13039\/100014013","name":"UK Research and Innovation award","doi-asserted-by":"publisher","award":["207684\/Z\/17\/Z"],"award-info":[{"award-number":["207684\/Z\/17\/Z"]}],"id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014013","name":"UK Research and Innovation award","doi-asserted-by":"publisher","award":["MR\/S03241X\/1"],"award-info":[{"award-number":["MR\/S03241X\/1"]}],"id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014013","name":"UK Research and Innovation award","doi-asserted-by":"publisher","award":["BB\/V010344\/1"],"award-info":[{"award-number":["BB\/V010344\/1"]}],"id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Biotechnology and Biological Sciences Research Council","award":["207684\/Z\/17\/Z"],"award-info":[{"award-number":["207684\/Z\/17\/Z"]}]},{"name":"Biotechnology and Biological Sciences Research Council","award":["MR\/S03241X\/1"],"award-info":[{"award-number":["MR\/S03241X\/1"]}]},{"name":"Biotechnology and Biological Sciences Research Council","award":["BB\/V010344\/1"],"award-info":[{"award-number":["BB\/V010344\/1"]}]},{"name":"University of Leeds (Leeds Anniversary Research Scholarship)","award":["207684\/Z\/17\/Z"],"award-info":[{"award-number":["207684\/Z\/17\/Z"]}]},{"name":"University of Leeds (Leeds Anniversary Research Scholarship)","award":["MR\/S03241X\/1"],"award-info":[{"award-number":["MR\/S03241X\/1"]}]},{"name":"University of Leeds (Leeds Anniversary Research Scholarship)","award":["BB\/V010344\/1"],"award-info":[{"award-number":["BB\/V010344\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Coordinated events of calcium (Ca2+) released from the endoplasmic reticulum (ER) are key second messengers in excitable cells. In pain-sensing dorsal root ganglion (DRG) neurons, these events can be observed as Ca2+ sparks, produced by a combination of ryanodine receptors (RyR) and inositol 1,4,5-triphosphate receptors (IP3R1). These microscopic signals offer the neuronal cells with a possible means of modulating the subplasmalemmal Ca2+ handling, initiating vesicular exocytosis. With super-resolution dSTORM and expansion microscopies, we visualised the nanoscale distributions of both RyR and IP3R1 that featured loosely organised clusters in the subplasmalemmal regions of cultured rat DRG somata. We adapted a novel correlative microscopy protocol to examine the nanoscale patterns of RyR and IP3R1 in the locality of each Ca2+ spark. We found that most subplasmalemmal sparks correlated with relatively small groups of RyR whilst larger sparks were often associated with larger groups of IP3R1. These data also showed spontaneous Ca2+ sparks in &lt;30% of the subplasmalemmal cell area but consisted of both these channel species at a 3.8\u20135 times higher density than in nonactive regions of the cell. Taken together, these observations reveal distinct patterns and length scales of RyR and IP3R1 co-clustering at contact sites between the ER and the surface plasmalemma that encode the positions and the quantity of Ca2+ released at each Ca2+ spark.<\/jats:p>","DOI":"10.3390\/cells13010038","type":"journal-article","created":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T20:44:15Z","timestamp":1703450655000},"page":"38","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Super-Resolution Analysis of the Origins of the Elementary Events of ER Calcium Release in Dorsal Root Ganglion Neurons"],"prefix":"10.3390","volume":"13","author":[{"given":"Miriam E.","family":"Hurley","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shihab S.","family":"Shah","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M. D.","family":"Sheard","sequence":"additional","affiliation":[{"name":"School of Biosciences, Faculty of Science, The University of Sheffield, Sheffield S10 2TN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah M.","family":"Kirton","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek S.","family":"Steele","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikita","family":"Gamper","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2461-478X","authenticated-orcid":false,"given":"Izzy","family":"Jayasinghe","sequence":"additional","affiliation":[{"name":"School of Biosciences, Faculty of Science, The University of Sheffield, Sheffield S10 2TN, UK"},{"name":"EMBL Australia Node in Single Molecule Science, School of Biomedical Science, University of New South Wales, Kensington, Sydney 2052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1038\/415198a","article-title":"Cardiac excitation-contraction coupling","volume":"415","author":"Bers","year":"2002","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"11024","DOI":"10.1073\/pnas.191352298","article-title":"Calcium regulation of neuronal gene expression","volume":"98","author":"West","year":"2001","journal-title":"Proc. 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