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Program","award":["2022B1111080007"],"award-info":[{"award-number":["2022B1111080007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Due to the increasing trend of delayed childbirth, the age-related decline in male reproductive function has become a widely recognized issue. Sertoli cells (SCs) play a vital role in creating the necessary microenvironment for spermatogenesis in the testis. However, the mechanism underlying Sertoli cell aging is still unclear. In this study, senescent Sertoli cells showed a substantial upregulation of miR-143-3p expression. miR-143-3p was found to limit Sertoli cell proliferation, promote cellular senescence, and cause blood\u2013testis barrier (BTB) dysfunction by targeting ubiquitin-conjugating enzyme E2 E3 (UBE2E3). Additionally, the TGF-\u03b2 receptor inhibitor SB431542 showed potential in alleviating age-related BTB dysfunction, rescuing testicular atrophy, and reversing the reduction in germ cell numbers by negatively regulating miR-143-3p. These findings clarified the regulatory pathways underlying Sertoli cell senescence and suggested a promising therapeutic approach to restore BTB function, alleviate Sertoli cell senescence, and improve reproductive outcomes for individuals facing fertility challenges.<\/jats:p>","DOI":"10.3390\/cells13040313","type":"journal-article","created":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T09:00:02Z","timestamp":1707382802000},"page":"313","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Inhibition of miR-143-3p Restores Blood\u2013Testis Barrier Function and Ameliorates Sertoli Cell Senescence"],"prefix":"10.3390","volume":"13","author":[{"given":"Ziyan","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlian","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8440-0890","authenticated-orcid":false,"given":"Rufei","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Mei","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingxian","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7835-4309","authenticated-orcid":false,"given":"Yan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Jinan University, Guangzhou 510632, China"},{"name":"Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yadong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Jinan University, Guangzhou 510632, China"},{"name":"Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1038\/aja.2013.92","article-title":"The effects of advanced paternal age on fertility","volume":"15","author":"Kovac","year":"2013","journal-title":"Asian J. 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