{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:07:56Z","timestamp":1787170076592,"version":"build-2736575974"},"reference-count":48,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T00:00:00Z","timestamp":1709078400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["310030_182528"],"award-info":[{"award-number":["310030_182528"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["SKINTEGRITY.CH"],"award-info":[{"award-number":["SKINTEGRITY.CH"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Swiss Federal Institute of Technology (ETH) Zurich","award":["310030_182528"],"award-info":[{"award-number":["310030_182528"]}]},{"name":"Swiss Federal Institute of Technology (ETH) Zurich","award":["SKINTEGRITY.CH"],"award-info":[{"award-number":["SKINTEGRITY.CH"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Dendritic cell (DC) migration from peripheral tissues via afferent lymphatic vessels to draining lymph nodes (dLNs) is important for the organism\u2019s immune regulation and immune protection. Several lymphatic endothelial cell (LEC)-expressed adhesion molecules have thus far been found to support transmigration and movement within the lymphatic vasculature. In this study, we investigated the contribution of CD112, an adhesion molecule that we recently found to be highly expressed in murine LECs, to this process. Performing in vitro assays in the murine system, we found that transmigration of bone marrow-derived dendritic cells (BM-DCs) across or adhesion to murine LEC monolayers was reduced when CD112 was absent on LECs, DCs, or both cell types, suggesting the involvement of homophilic CD112\u2013CD112 interactions. While CD112 was highly expressed in murine dermal LECs, CD112 levels were low in endogenous murine dermal DCs and BM-DCs. This might explain why we observed no defect in the in vivo lymphatic migration of adoptively transferred BM-DCs or endogenous DCs from the skin to dLNs. Compared to murine DCs, human monocyte-derived DCs expressed higher CD112 levels, and their migration across human CD112-expressing LECs was significantly reduced upon CD112 blockade. CD112 expression was also readily detected in endogenous human dermal DCs and LECs by flow cytometry and immunofluorescence. Upon incubating human skin punch biopsies in the presence of CD112-blocking antibodies, DC emigration from the tissue into the culture medium was significantly reduced, indicating impaired lymphatic migration. Overall, our data reveal a contribution of CD112 to human DC migration.<\/jats:p>","DOI":"10.3390\/cells13050424","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T09:26:17Z","timestamp":1709112377000},"page":"424","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["CD112 Supports Lymphatic Migration of Human Dermal Dendritic Cells"],"prefix":"10.3390","volume":"13","author":[{"given":"Neda","family":"Haghayegh Jahromi","sequence":"first","affiliation":[{"name":"Institute of Pharmaceutical Sciences, ETH Zurich, Vladimir-Prelog-Weg 1-5\/10, 8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia-Olga","family":"Gkountidi","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, ETH Zurich, Vladimir-Prelog-Weg 1-5\/10, 8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Collado-Diaz","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, ETH Zurich, Vladimir-Prelog-Weg 1-5\/10, 8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Blatter","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, ETH Zurich, Vladimir-Prelog-Weg 1-5\/10, 8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Bauer","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, ETH Zurich, Vladimir-Prelog-Weg 1-5\/10, 8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lito","family":"Zambounis","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, ETH Zurich, Vladimir-Prelog-Weg 1-5\/10, 8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica Danielly","family":"Medina-Sanchez","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, ETH Zurich, Vladimir-Prelog-Weg 1-5\/10, 8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Russo","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, ETH Zurich, Vladimir-Prelog-Weg 1-5\/10, 8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Runge","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, ETH Zurich, Vladimir-Prelog-Weg 1-5\/10, 8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4364-2364","authenticated-orcid":false,"given":"Gaetana","family":"Restivo","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Epameinondas","family":"Gousopoulos","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, Raemistrasse 100, 8091 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Lindenblatt","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, Raemistrasse 100, 8091 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5902-9420","authenticated-orcid":false,"given":"Mitchell P.","family":"Levesque","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7899-2850","authenticated-orcid":false,"given":"Cornelia","family":"Halin","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, ETH Zurich, Vladimir-Prelog-Weg 1-5\/10, 8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"603","DOI":"10.3389\/fimmu.2015.00603","article-title":"T Lymphocyte-Endothelial Interactions: Emerging Understanding of Trafficking and Antigen-Specific Immunity","volume":"6","author":"Carman","year":"2015","journal-title":"Front. 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