{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T03:35:47Z","timestamp":1772595347421,"version":"3.50.1"},"reference-count":81,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Nasopharyngeal carcinoma (NPC) is a type of cancer that originates from the mucosal lining of the nasopharynx and can invade and spread. Although contemporary chemoradiotherapy effectively manages the disease locally, there are still challenges with locoregional recurrence and distant failure. Therefore, it is crucial to have a deeper understanding of the molecular basis of NPC cell movement in order to develop a more effective treatment and to improve patient survival rates. Cancer cell line models are invaluable in studying health and disease and it is not surprising that they play a critical role in NPC research. Consequently, scientists have established around 80 immortalized human NPC lines that are commonly used as in vitro models. However, over the years, it has been observed that many cell lines are misidentified or contaminated by other cells. This cross-contamination leads to the creation of false cell lines that no longer match the original donor. In this commentary, we discuss the impact of misidentified NPC cell lines on the scientific literature. We found 1159 articles from 2000 to 2023 that used NPC cell lines contaminated with HeLa cells. Alarmingly, the number of publications and citations using these contaminated cell lines continued to increase, even after information about the contamination was officially published. These articles were most commonly published in the fields of oncology, pharmacology, and experimental medicine research. These findings highlight the importance of science policy and support the need for journals to require authentication testing before publication.<\/jats:p>","DOI":"10.3390\/cells13070559","type":"journal-article","created":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T10:03:59Z","timestamp":1711101839000},"page":"559","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Nasopharyngeal Carcinoma Cell Lines: Reliable Alternatives to Primary Nasopharyngeal Cells?"],"prefix":"10.3390","volume":"13","author":[{"given":"Anna","family":"Makowska","sequence":"first","affiliation":[{"name":"Division of Pediatric Hematology, Oncology and Stem Cell Transplantation, RWTH University Hospital Aachen, D-52074 Aachen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3888-0931","authenticated-orcid":false,"given":"Ralf","family":"Weiskirchen","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, D-52074 Aachen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/S0140-6736(19)30956-0","article-title":"Nasopharyngeal carcinoma","volume":"394","author":"Chen","year":"2019","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3322\/caac.21492","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA Cancer J. Clin."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"115","DOI":"10.3322\/caac.21338","article-title":"Cancer statistics in China, 2015","volume":"66","author":"Chen","year":"2016","journal-title":"CA Cancer J. Clin."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1038\/s41374-022-00817-4","article-title":"Overexpression of KITLG predicts unfavorable clinical outcomes and promotes lymph node metastasis via the JAK\/STAT pathway in nasopharyngeal carcinoma","volume":"102","author":"Ling","year":"2022","journal-title":"Lab. Investig."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e49968","DOI":"10.2196\/49968","article-title":"Nasopharyngeal cancer incidence and mortality in 185 countries in 2020 and the projected burden in 2040: Population-based global epidemiological profiling","volume":"9","author":"Zhang","year":"2023","journal-title":"JMIR Public Health Surveill."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.7150\/ijbs.59688","article-title":"The role of exosomes in the pathogenesis of nasopharyngeal carcinoma and the involved clinical application","volume":"17","author":"Luo","year":"2021","journal-title":"Int. J. Biol. Sci."},{"key":"ref_7","first-page":"e15","article-title":"Review of the histological classification of nasopharyngeal carcinoma","volume":"1","author":"Li","year":"2014","journal-title":"J. Nasopharyng. Carcinoma"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1007\/s10555-017-9693-x","article-title":"Progression of understanding for the role of Epstein-Barr virus and management of nasopharyngeal carcinoma","volume":"36","author":"Nakanishi","year":"2017","journal-title":"Cancer Metastasis Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1083\/jcb.129.1.147","article-title":"Effects of CapG overexpression on agonist-induced motility and second messenger generation","volume":"129","author":"Sun","year":"1995","journal-title":"J. Cell Biol."},{"key":"ref_10","first-page":"2931","article-title":"Comparison of features of human breast cancer cell lines and their corresponding tumors","volume":"4","author":"Wistuba","year":"1998","journal-title":"Clin. Cancer Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/73432","article-title":"Systematic variation in gene expression patterns in human cancer cell lines","volume":"24","author":"Ross","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/ijc.25242","article-title":"Check your cultures! A list of cross-contaminated or misidentified cell lines","volume":"127","author":"Theodosopoulos","year":"2010","journal-title":"Int. J. Cancer"},{"key":"ref_13","unstructured":"(2024, March 16). Cellosaurus\u2014A Knowledge Resource on Cell Lines. Available online: https:\/\/www.cellosaurus.org\/."},{"key":"ref_14","first-page":"430","article-title":"Differentially expressed gene in nasopharyngeal carcinoma cell lines with various metastatic potentialities","volume":"24","author":"Zhang","year":"2002","journal-title":"Zhonghua Zhong Liu Za Zhi"},{"key":"ref_15","first-page":"190","article-title":"Transfer of EBV (Epstein-Barr virus) genome from lymphoblastoid cells to established epithelial cells derived from human tissues Experimental studies on the viral etiology of nasopharyngeal carcinoma (NPC) related to EBV infection","volume":"84","author":"Furukawa","year":"1975","journal-title":"Kanazawa Daigaku Juzen Igakkai Zasshi"},{"key":"ref_16","first-page":"575","article-title":"Relationship between the insulin-like growth factor 1 receptor signaling pathway and the resistance of nasopharyngeal carcinoma to cetuximab","volume":"32","author":"Zuo","year":"2010","journal-title":"Zhonghua Zhong Liu Za Zhi"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1017\/S0022215100094883","article-title":"Establishment of Epstein-Barr virus (EBV) infection in epithelial cells derived from the nasopharynx by cell fusion","volume":"97","author":"Takimoto","year":"1983","journal-title":"J. Laryngol. Otol."},{"key":"ref_18","first-page":"711","article-title":"Tumorigenicity of nasopharyngeal carcinoma hybrid cell line","volume":"73","author":"Takimoto","year":"1984","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1038\/s41374-018-0034-7","article-title":"Establishment of a nasopharyngeal carcinoma cell line capable of undergoing lytic Epstein-Barr virus reactivation","volume":"98","author":"Yip","year":"2018","journal-title":"Lab. Investig."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0165-4608(97)00231-8","article-title":"Characterization of a new EBV-associated nasopharyngeal carcinoma cell line","volume":"101","author":"Hui","year":"1998","journal-title":"Cancer Genet. Cytogenet."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1002\/(SICI)1097-0215(19990924)83:1<121::AID-IJC21>3.0.CO;2-F","article-title":"Nasopharyngeal carcinoma cell line (C666-1) consistently harbouring Epstein-Barr virus","volume":"83","author":"Cheung","year":"1999","journal-title":"Int. J. Cancer"},{"key":"ref_22","first-page":"6752","article-title":"Establishment and characterization of a tumor cell line from human nasopharyngeal carcinoma tissue","volume":"49","author":"Chang","year":"1989","journal-title":"Cancer Res."},{"key":"ref_23","unstructured":"(1978). Establishment of an epitheloid cell line and a fusiform cell line from a patient with nasopharyngeal carcinoma. Sci. Sin., 21, 127\u2013134."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1002\/ijc.2910310509","article-title":"Cytogenetic studies on an epithelial cell line derived from poorly differentiated nasopharyngeal carcinoma","volume":"31","author":"Sizhong","year":"1983","journal-title":"Int. J. Cancer"},{"key":"ref_25","first-page":"288","article-title":"The invasive behavior of two human nasopharyngeal carcinoma (NPC) cell lines CNE-1, CNE-2Z in vitro","volume":"20","author":"Kong","year":"1991","journal-title":"Zhonghua Bing. Li Xue Za Zhi"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1007\/BF01209487","article-title":"Suppression of human nasopharyngeal carcinoma cell growth in nude mice by the wild-type p53 gene","volume":"119","author":"Chen","year":"1992","journal-title":"J. Cancer Res. Clin. Oncol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e1224","DOI":"10.1002\/cnr2.1224","article-title":"CRISPR\/Cas9-mediated double knockout of SRPK1 and SRPK2 in a nasopharyngeal carcinoma cell line","volume":"3","author":"Prattapong","year":"2020","journal-title":"Cancer Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3450","DOI":"10.1158\/0008-5472.CAN-09-4099","article-title":"Identification of biomarkers for predicting nasopharyngeal carcinoma response to radiotherapy by proteomics","volume":"70","author":"Feng","year":"2010","journal-title":"Cancer Res."},{"key":"ref_29","first-page":"1300","article-title":"Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines","volume":"28","author":"Klein","year":"1968","journal-title":"Cancer Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"9524","DOI":"10.1073\/pnas.86.23.9524","article-title":"Two epithelial tumor cell lines (HNE-1 and HONE-1) latently infected with Epstein-Barr virus that were derived from nasopharyngeal carcinomas","volume":"86","author":"Glaser","year":"1989","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2169","DOI":"10.1002\/ijc.23374","article-title":"Authentication of nasopharyngeal carcinoma tumor lines","volume":"122","author":"Chan","year":"2008","journal-title":"Int. J. Cancer"},{"key":"ref_32","first-page":"3716","article-title":"Novel mutation of topoisomerase I in rendering cells resistant to camptothecin","volume":"62","author":"Chang","year":"2002","journal-title":"Cancer Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1002\/ijc.2910060206","article-title":"Nasopharyngeal carcinoma (NPC). I. Types of cultures derived from tumour biopsies and non-tumorous tissues of Chinese patients with special reference to lymphoblastoid transformation","volume":"6","author":"Ho","year":"1970","journal-title":"Int. J. Cancer"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1002\/ijc.2910040604","article-title":"Some factors affecting membrane immunofluorescence reactivity of Burkitt lymphoma tissue culture cell lines","volume":"4","author":"Yata","year":"1969","journal-title":"Int. J. Cancer."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"E3473","DOI":"10.1073\/pnas.1202637109","article-title":"Cyclin D1 overexpression supports stable EBV infection in nasopharyngeal epithelial cells","volume":"109","author":"Tsang","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/S0167-4889(02)00208-2","article-title":"Establishment of two immortalized nasopharyngeal epithelial cell lines using SV40 large T and HPV16E6\/E7 viral oncogenes","volume":"1590","author":"Tsao","year":"2002","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1002\/ijc.22032","article-title":"Molecular and cytogenetic changes involved in the immortalization of nasopharyngeal epithelial cells by telomerase","volume":"119","author":"Li","year":"2006","journal-title":"Int. J. Cancer"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"453","DOI":"10.2183\/pjab1945.46.453","article-title":"Presence of herpes-type virus in the culture cell line from a nasopharyngeal carcinoma in Taiwan","volume":"46","author":"Sugano","year":"1970","journal-title":"Proc. Jpn. Acad. B Phys. Biol. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Li, H.P., Huang, C.Y., Lui, K.W., Chao, Y.K., Yeh, C.N., Lee, L.Y., Huang, Y., Lin, T.L., Kuo, Y.C., and Huang, M.Y. (2021). Combination of epithelial growth factor receptor blockers and CDK4\/6 inhibitor for nasopharyngeal carcinoma treatment. Cancers, 13.","DOI":"10.3390\/cancers13122954"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1158\/2767-9764.CRC-23-0341","article-title":"Establishment and characterization of an Epstein-Barr virus-positive cell line from a non-keratinizing differentiated primary nasopharyngeal carcinoma","volume":"4","author":"Chai","year":"2024","journal-title":"Cancer Res. Commun."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Lin, J.C., Liao, S.K., Lee, E.H., Hung, M.S., Sayion, Y., Chen, H.C., Kang, C.C., Huang, L.S., and Cherng, J.M. (2009). Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome. J. Biomed. Sci., 16.","DOI":"10.1186\/1423-0127-16-105"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/S0165-4608(97)00416-0","article-title":"Chromosomal abnormalities of a new nasopharyngeal carcinoma cell line (NPC-BM1) derived from a bone marrow metastatic lesion","volume":"103","author":"Liao","year":"1998","journal-title":"Cancer Genet. Cytogenet."},{"key":"ref_43","first-page":"713","article-title":"Establishment and characterization of two nasopharyngeal carcinoma cell lines","volume":"62","author":"Lin","year":"1990","journal-title":"Lab. Investig."},{"key":"ref_44","first-page":"716","article-title":"Characterization of seven newly established nasopharyngeal carcinoma cell lines","volume":"68","author":"Lin","year":"1993","journal-title":"Lab. Investig."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1038\/labinvest.3780035","article-title":"Epstein-Barr virus infection to Epstein-Barr virus-negative nasopharyngeal carcinoma cell line TW03 enhances its tumorigenicity","volume":"80","author":"Teramoto","year":"2000","journal-title":"Lab. Investig."},{"key":"ref_46","first-page":"329","article-title":"Establishment and characterization of nasopharyngeal carcinoma cell line","volume":"34","author":"Yanoma","year":"1988","journal-title":"Jibi Rinsho"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1002\/ijc.2910260202","article-title":"Establishment of a cell line (NPC\/HK1) from a differentiated squamous carcinoma of the nasopharynx","volume":"26","author":"Huang","year":"1980","journal-title":"Int. J. Cancer"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"4663","DOI":"10.1038\/s41467-018-06889-5","article-title":"Establishment and characterization of new tumor xenografts and cancer cell lines from EBV-positive nasopharyngeal carcinoma","volume":"9","author":"Lin","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_49","first-page":"161","article-title":"Surface antigens on lymphoblastoid cells derived from nasopharyngeal carcinoma","volume":"7","author":"Klein","year":"1970","journal-title":"Clin. Exp. Immunol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1159\/000149930","article-title":"An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection","volume":"5","author":"Klein","year":"1975","journal-title":"Intervirology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0385-8146(87)80025-1","article-title":"Establishment of an Epstein-Barr virus (EBV) genome-positive subline of Ramos (Ramos\/NPC) following infection of Ramos with nasopharyngeal carcinoma (NPC)-derived EBV","volume":"14","author":"Takimoto","year":"1987","journal-title":"Auris Nasus Larynx"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"10365","DOI":"10.1158\/0008-5472.CAN-06-2977","article-title":"Preparing the \u201csoil\u201d: The primary tumor induces vasculature reorganization in the sentinel lymph node before the arrival of metastatic cancer cells","volume":"66","author":"Qian","year":"2006","journal-title":"Cancer Res."},{"key":"ref_53","unstructured":"Li, C.Z., Qian, C.N., Chen, J.D., Zhou, H.J., and Hao, D.L. (2019). A Visualized Nasopharyngeal Carcinoma Cell and Its Application. (CN109536452A)."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4268","DOI":"10.1096\/fj.14-266718","article-title":"Genetic profiling reveals an alarming rate of cross-contamination among human cell lines used in China","volume":"29","author":"Ye","year":"2015","journal-title":"FASEB J."},{"key":"ref_55","first-page":"36","article-title":"Establishment and characterization of a novel nasopharyngeal carcinoma cell line (SUNE2) from a Cantonese patient","volume":"31","author":"Dong","year":"2012","journal-title":"Chin. J. Cancer"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/356347a0","article-title":"Epstein-Barr virus infection and replication in a human epithelial cell system","volume":"356","author":"Li","year":"1992","journal-title":"Nature"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"American Type Culture Collection Standards Development Organization Workgroup ASN-0002 (2010). Cell line misidentification: The beginning of the end. Nat. Rev. Cancer, 10, 441\u2013448.","DOI":"10.1038\/nrc2852"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1002\/bit.28496","article-title":"Passage number of cancer cell lines: Importance, intricacies, and way-forward","volume":"120","author":"Prasad","year":"2023","journal-title":"Biotechnol. Bioeng."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"575","DOI":"10.2144\/000112598","article-title":"The costs of using unauthenticated, over-passaged cell lines: How much more data do we need?","volume":"43","author":"Hughes","year":"2007","journal-title":"Biotechniques"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1159\/000152408","article-title":"G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells","volume":"21","author":"Beckman","year":"1971","journal-title":"Hum. Hered."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1126\/science.6451928","article-title":"Cross-contamination of cells in culture","volume":"212","author":"Daniels","year":"1981","journal-title":"Science"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1177\/1933719114522518","article-title":"Discovery of HeLa cell contamination in HES cells: Call for cell line authentication in reproductive biology research","volume":"21","author":"Kniss","year":"2014","journal-title":"Reprod. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1290\/1543-706X(2004)40<211:CLCHAA>2.0.CO;2","article-title":"Cell line cross-contamination: How aware are Mammalian cell culturists of the problem and how to monitor it?","volume":"40","author":"Buehring","year":"2004","journal-title":"Vitr. Cell. Dev. Biol. Anim."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Butler, J.M. (2015). The future of forensic DNA analysis. Philos. Trans. R. Soc. Lond. B Biol. Sci., 370.","DOI":"10.1098\/rstb.2014.0252"},{"key":"ref_65","unstructured":"Markossian, S., Grossman, A., Brimacombe, K., Arkin, M., Auld, D., Austin, C., Baell, J., Chung, T.D.Y., Coussens, N.P., and Dahlin, J.L. (2024, March 16). Authentication of human and mouse cell lines by short tandem repeat (STR) DNA genotype analysis, Assay Guidance Manual [Internet], Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK144066\/."},{"key":"ref_66","unstructured":"(2012). Authentication of Human Cell Lines: Standardization of STR Profiling (Standard No. ASN-0002). Available online: https:\/\/webstore.ansi.org\/preview-pages\/ATCC\/preview_ANSI+ATCC+ASN-0002-2011.pdf."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s10616-006-9013-8","article-title":"Identity tests: Determination of cell line cross-contamination","volume":"51","author":"Cabrera","year":"2006","journal-title":"Cytotechnology"},{"key":"ref_68","first-page":"359","article-title":"Immunological and karyological criteria for identification of cell lines","volume":"25","author":"Defendi","year":"1960","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_69","first-page":"149","article-title":"Efficient DNA fingerprinting method for the identification of cross-culture contamination of cell lines","volume":"12","author":"Matsuo","year":"1999","journal-title":"Hum. Cell"},{"key":"ref_70","first-page":"158","article-title":"Molecular mechanisms of tumorgenesis and metastasis in nasopharyngeal carcinoma cell sublines","volume":"21","author":"Song","year":"2002","journal-title":"Ai Zheng"},{"key":"ref_71","first-page":"330","article-title":"Effect of EBV latent membrane protein 1 gene isolated from human nasopharyngeal carcinoma cell line SUNE on the growth of immortalized epithelial cells","volume":"20","author":"Chen","year":"1998","journal-title":"Zhonghua Zhong Liu Za Zhi"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2784","DOI":"10.1158\/0008-5472.CAN-16-2258","article-title":"Widespread use of misidentified cell line KB (HeLa): Incorrect attribution and its impact revealed through mining the scientific literature","volume":"77","author":"Vaughan","year":"2017","journal-title":"Cancer Res."},{"key":"ref_73","unstructured":"(2024, March 16). Web of Science. Available online: https:\/\/www.webofscience.com."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1007\/s11626-016-0124-z","article-title":"Are your results valid? Cellular authentication a need from the past, an emergency on the present","volume":"53","author":"Cosme","year":"2017","journal-title":"Vitr. Cell. Dev. Biol. Anim."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1158\/1055-9965.EPI-08-1231","article-title":"Human biospecimen research: Experimental protocol and quality control tools","volume":"18","author":"Betsou","year":"2009","journal-title":"Cancer Epidemiol. Biomarkers Prev."},{"key":"ref_76","unstructured":"(2024, March 16). ICLAC Register of Misidentified Cell Lines. Available online: https:\/\/iclac.org\/."},{"key":"ref_77","unstructured":"(2024, March 16). Cellosaurus. Description of the Cellosaurus\u2014A Knowledge Resource on Cell Lines. Available online: web.expasy.org\/cellosaurus\/description.html."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"152900","DOI":"10.1016\/j.tox.2021.152900","article-title":"Reproducibility of adipogenic responses to metabolism disrupting chemicals in the 3T3-L1 pre-adipocyte model system: An interlaboratory study","volume":"461","author":"Kassotis","year":"2021","journal-title":"Toxicology"},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Almeida, J.L., Cole, K.D., and Plant, A.L. (2016). Standards for cell line authentication and beyond. PLoS Biol., 14.","DOI":"10.1371\/journal.pbio.1002476"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Weiskirchen, S., Schr\u00f6der, S.K., Buhl, E.M., and Weiskirchen, R. (2023). A beginner\u2019s guide to cell culture: Practical advice for preventing needless problems. Cells, 12.","DOI":"10.3390\/cells12050682"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"171","DOI":"10.3390\/livers2030015","article-title":"Established liver cell lines: Are you sure to have the right ones?","volume":"2","author":"Weiskirchen","year":"2022","journal-title":"Livers"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/13\/7\/559\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:17:48Z","timestamp":1760105868000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/13\/7\/559"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,22]]},"references-count":81,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["cells13070559"],"URL":"https:\/\/doi.org\/10.3390\/cells13070559","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,22]]}}}