{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T03:44:21Z","timestamp":1774323861296,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Next Generation EU-Italian NRRP, Mission 4, Component 2, Investment 1.5","award":["2021\/3277"],"award-info":[{"award-number":["2021\/3277"]}]},{"name":"Next Generation EU-Italian NRRP, Mission 4, Component 2, Investment 1.5","award":["ECS0000009"],"award-info":[{"award-number":["ECS0000009"]}]},{"name":"Territorial R&amp;D Leaders","award":["2021\/3277"],"award-info":[{"award-number":["2021\/3277"]}]},{"name":"Territorial R&amp;D Leaders","award":["ECS0000009"],"award-info":[{"award-number":["ECS0000009"]}]},{"name":"Tech4You-Technologies for climate change adaptation and quality of life improvement","award":["2021\/3277"],"award-info":[{"award-number":["2021\/3277"]}]},{"name":"Tech4You-Technologies for climate change adaptation and quality of life improvement","award":["ECS0000009"],"award-info":[{"award-number":["ECS0000009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>During pregnancy, uterine vasculature undergoes significant circumferential growth to increase uterine blood flow, vital for the growing feto-placental unit. However, this process is often compromised in conditions like maternal high blood pressure, particularly in preeclampsia (PE), leading to fetal growth impairment. Currently, there is no cure for PE, partly due to the adverse effects of anti-hypertensive drugs on maternal and fetal health. This study aimed to investigate the vasodilator effect of extra virgin olive oil (EVOO) phenols on the reproductive vasculature, potentially benefiting both mother and fetus. Isolated uterine arteries (UAs) from pregnant rats were tested with EVOO phenols in a pressurized myograph. To elucidate the underlying mechanisms, additional experiments were conducted with specific inhibitors: L-NAME\/L-NNA (10\u22124 M) for nitric oxide synthases, ODQ (10\u22125 M) for guanylate cyclase, Verapamil (10\u22125 M) for the L-type calcium channel, Ryanodine (10\u22125 M) + 2-APB (3 \u00d7 10\u22125 M) for ryanodine and the inositol triphosphate receptors, respectively, and Paxilline (10\u22125 M) for the large-conductance calcium-activated potassium channel. The results indicated that EVOO-phenols activate Ca2+ signaling pathways, generating nitric oxide, inducing vasodilation via cGMP and BKCa2+ signals in smooth muscle cells. This study suggests the potential use of EVOO phenols to prevent utero-placental blood flow restriction, offering a promising avenue for managing PE.<\/jats:p>","DOI":"10.3390\/cells13070619","type":"journal-article","created":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:58:38Z","timestamp":1712105918000},"page":"619","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Dilation of Pregnant Rat Uterine Arteries with Phenols from Extra Virgin Olive Oil Is Endothelium-Dependent and Involves Calcium and Potassium Channels"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2326-5353","authenticated-orcid":false,"given":"Milena","family":"Esposito","sequence":"first","affiliation":[{"name":"Department of Biology, Ecology & Earth Science, University of Calabria, 87036 Arcavacata di Rende, CS, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariacarmela","family":"Gatto","sequence":"additional","affiliation":[{"name":"Department of Biology, Ecology & Earth Science, University of Calabria, 87036 Arcavacata di Rende, CS, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9172-5941","authenticated-orcid":false,"given":"Marilyn J.","family":"Cipolla","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences, University of Vermont, Larner College of Medicine, Burlington, VT 05405, USA"},{"name":"Department of Neurological Sciences, University of Vermont, Larner College of Medicine, Burlington, VT 05405, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ira M.","family":"Bernstein","sequence":"additional","affiliation":[{"name":"Department of Neurological Sciences, University of Vermont, Larner College of Medicine, Burlington, VT 05405, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3736-0205","authenticated-orcid":false,"given":"Maurizio","family":"Mandal\u00e0","sequence":"additional","affiliation":[{"name":"Department of Biology, Ecology & Earth Science, University of Calabria, 87036 Arcavacata di Rende, CS, Italy"},{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences, University of Vermont, Larner College of Medicine, Burlington, VT 05405, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1097\/00000542-196507000-00004","article-title":"Changes in the blood volume during pregnancy and delivery","volume":"26","author":"Pritchard","year":"1965","journal-title":"Anesthesiology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1097\/HJH.0b013e32834f0b1c","article-title":"Changes in blood pressure during healthy pregnancy: A longitudinal cohort study","volume":"30","author":"Grindheim","year":"2012","journal-title":"J. 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