{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T14:11:47Z","timestamp":1784729507103,"version":"3.55.0"},"reference-count":46,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T00:00:00Z","timestamp":1712620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Subarachnoid hemorrhage (SAH) remains a major cause of cerebrovascular morbidity, eliciting severe headaches and vasospasms that have been shown to inversely correlate with vasodilator calcitonin gene-related peptide (CGRP) levels. Although dura mater trigeminal afferents are an important source of intracranial CGRP, little is known about the effects of SAH on these neurons in preclinical models. The present study evaluated changes in CGRP levels and expression in trigeminal primary afferents innervating the dura mater 72 h after experimentally induced SAH in adult rats. SAH, eliciting marked damage revealed by neurological examination, significantly reduced the density of CGRP-immunoreactive nerve fibers both in the dura mater and the trigeminal caudal nucleus in the medulla but did not affect the total dural nerve fiber density. SAH attenuated ex vivo dural CGRP release by ~40% and in the trigeminal ganglion, reduced both CGRP mRNA levels and the number of highly CGRP-immunoreactive cell bodies. In summary, we provide novel complementary evidence that SAH negatively affects the integrity of the CGRP-expressing rat trigeminal neurons. Reduced CGRP levels suggest likely impaired meningeal neurovascular functions contributing to SAH complications. Further studies are to be performed to reveal the importance of impaired CGRP synthesis and its consequences in central sensory processing.<\/jats:p>","DOI":"10.3390\/cells13080653","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T02:52:17Z","timestamp":1712631137000},"page":"653","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Subarachnoid Hemorrhage Depletes Calcitonin Gene-Related Peptide Levels of Trigeminal Neurons in Rat Dura Mater"],"prefix":"10.3390","volume":"13","author":[{"given":"Thannoon","family":"Masood","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Albert Szent-Gy\u00f6rgyi Medical School, University of Szeged, Semmelweis utca 6., H-6725 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Szandra","family":"Lakatos","sequence":"additional","affiliation":[{"name":"Department of Physiology, Albert Szent-Gy\u00f6rgyi Medical School, University of Szeged, D\u00f3m t\u00e9r 10., H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gy\u00f6ngyi","family":"Kis","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, K\u00f6z\u00e9p fasor 52., H-6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9154-0885","authenticated-orcid":false,"given":"Melissza","family":"Ign\u00e1cz","sequence":"additional","affiliation":[{"name":"Department of Physiology, Albert Szent-Gy\u00f6rgyi Medical School, University of Szeged, D\u00f3m t\u00e9r 10., H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5581-2167","authenticated-orcid":false,"given":"Ferenc","family":"Domoki","sequence":"additional","affiliation":[{"name":"Department of Physiology, Albert Szent-Gy\u00f6rgyi Medical School, University of Szeged, D\u00f3m t\u00e9r 10., H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judit","family":"Rosta","sequence":"additional","affiliation":[{"name":"Department of Physiology, Albert Szent-Gy\u00f6rgyi Medical School, University of Szeged, D\u00f3m t\u00e9r 10., H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1212\/WNL.10.6.555","article-title":"The tentorial nerves and Iocalization of intracranial pain in man","volume":"10","author":"Feindel","year":"1960","journal-title":"Neurology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"630","DOI":"10.3171\/jns.1971.34.5.0630","article-title":"Referral of pain from dural stimulation in man","volume":"34","author":"Wirth","year":"1971","journal-title":"J. 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