{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T15:46:04Z","timestamp":1776786364176,"version":"3.51.2"},"reference-count":75,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,6,8]],"date-time":"2024-06-08T00:00:00Z","timestamp":1717804800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014413","name":"University of Information Technology and Management in Rzeszow, Poland","doi-asserted-by":"publisher","award":["503-07-01-27"],"award-info":[{"award-number":["503-07-01-27"]}],"id":[{"id":"10.13039\/501100014413","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014413","name":"University of Information Technology and Management in Rzeszow, Poland","doi-asserted-by":"publisher","award":["503-07-01-59"],"award-info":[{"award-number":["503-07-01-59"]}],"id":[{"id":"10.13039\/501100014413","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014413","name":"University of Information Technology and Management in Rzeszow, Poland","doi-asserted-by":"publisher","award":["0121U100690"],"award-info":[{"award-number":["0121U100690"]}],"id":[{"id":"10.13039\/501100014413","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Health of Ukraine","award":["503-07-01-27"],"award-info":[{"award-number":["503-07-01-27"]}]},{"name":"Ministry of Health of Ukraine","award":["503-07-01-59"],"award-info":[{"award-number":["503-07-01-59"]}]},{"name":"Ministry of Health of Ukraine","award":["0121U100690"],"award-info":[{"award-number":["0121U100690"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Cancer is one of the most important problems of modern societies. Recently, studies have reported the anticancer properties of rosiglitazone related to its ability to bind peroxisome proliferator receptor \u03b3 (PPAR\u03b3), which has various effects on cancer and can inhibit cell proliferation. In this study, we investigated the effect of new 4-thiazolidinone (4-TZD) hybrids Les-4369 and Les-3467 and their effect on reactive oxygen species (ROS) production, metabolic activity, lactate dehydrogenase (LDH) release, caspase-3 activity, and gene and protein expression in human foreskin fibroblast (BJ) cells and lung adenocarcinoma (A549) cells. The ROS production and caspase-3 activity were mainly increased in the micromolar concentrations of the studied compounds in both cell lines. Les-3467 and Les-4369 increased the mRNA expression of PPARG, P53 (tumor protein P53), and ATM (ATM serine\/threonine kinase) in the BJ cells, while the mRNA expression of these genes (except PPARG) was mainly decreased in the A549 cells treated with both of the tested compounds. Our results indicate a decrease in the protein expression of AhR, PPAR\u03b3, and PARP-1 in the BJ cells exposed to 1 \u00b5M Les-3467 and Les-4369. In the A549 cells, the protein expression of AhR, PPAR\u03b3, and PARP-1 increased in the treatment with 1 \u00b5M Les-3467 and Les-4369. We have also shown the PPAR\u03b3 modulatory properties of Les-3467 and Les-4369. However, both compounds prove weak anticancer properties evidenced by their action at high concentrations and non-selective effects against BJ and A549 cells.<\/jats:p>","DOI":"10.3390\/cells13121007","type":"journal-article","created":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T08:49:03Z","timestamp":1718009343000},"page":"1007","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Role of 4-Thiazolidinone\u2013Pyrazoline\/Indoline Hybrids Les-4369 and Les-3467 in BJ and A549 Cell Lines"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6194-4792","authenticated-orcid":false,"given":"Karolina","family":"Kosi\u0144ska","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Cell Biology, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7833-9985","authenticated-orcid":false,"given":"Bartosz","family":"Sk\u00f3ra","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Cell Biology, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9892-437X","authenticated-orcid":false,"given":"Serhii","family":"Holota","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical, Organic and Bioorganic Chemistry, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine"},{"name":"Department of Organic Chemistry and Pharmacy, Lesya Ukrainka Volyn National University, Volya Avenue 13, 43025 Lutsk, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2125-3766","authenticated-orcid":false,"given":"Yulia","family":"Shepeta","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, National Pirogov Memorial Medical University, Pirogov 56, 21018 Vinnytsia, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4000-751X","authenticated-orcid":false,"given":"Anna","family":"Tab\u0119cka-\u0141onczy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Cell Biology, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3322-0080","authenticated-orcid":false,"given":"Roman","family":"Lesyk","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Cell Biology, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland"},{"name":"Department of Pharmaceutical, Organic and Bioorganic Chemistry, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2207-1160","authenticated-orcid":false,"given":"Konrad A.","family":"Szychowski","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Cell Biology, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.12703\/r\/12-22","article-title":"Targeted Therapies in Non-Small Cell Lung Cancer: Present and Future","volume":"12","author":"McLaughlin","year":"2023","journal-title":"Fac. 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