{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:40:39Z","timestamp":1791582039491,"version":"4.3.4"},"reference-count":79,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T00:00:00Z","timestamp":1722211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>In recent years, several studies have analyzed the composition of the male genital tract microbiota and its changes in infertility or in different situations associated with infertility. The aim of this narrative review is to obtain more insight on this topic; in particular, to describe actual evidence about changes in the semen microbiota in patients with infertility, male tract infections, or HPV infections. In semen, an increase in semen Prevotella spp. is associated with oligozoospermia and with obesity-associated asthenozoospermia; an increase in Pseudomonas is more frequently associated with asthenozoospermia and oligozoospermia; a reduction in Lactobacilli spp. (namely in Lactobacillus crispatus) may represent a marker of low semen quality. However, an increase in Lactobacillus iners is considered a risk factor for a reduced sperm concentration. In patients with prostatitis, there is a reduction in Lactobacillus spp. and an increase in Streptococcus spp., opening important perspectives about the role of probiotic treatments in these patients. Finally, an increase in Fusobacteria spp. was observed in patients with an HPV infection. In the conclusion, we underline the interactions between the seminal and vaginal microbiota, so that further studies should focus on the \u201ccouple genital microbiota\u201d.<\/jats:p>","DOI":"10.3390\/cells13151275","type":"journal-article","created":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T16:37:17Z","timestamp":1722271037000},"page":"1275","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Male Tract Microbiota and Male Infertility"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3264-0937","authenticated-orcid":false,"given":"Giuseppe","family":"Grande","sequence":"first","affiliation":[{"name":"Unit of Andrology and Reproductive Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3239-2374","authenticated-orcid":false,"given":"Andrea","family":"Graziani","sequence":"additional","affiliation":[{"name":"Unit of Andrology and Reproductive Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"De Toni","sequence":"additional","affiliation":[{"name":"Unit of Andrology and Reproductive Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4736-9051","authenticated-orcid":false,"given":"Andrea","family":"Garolla","sequence":"additional","affiliation":[{"name":"Unit of Andrology and Reproductive Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5817-8141","authenticated-orcid":false,"given":"Alberto","family":"Ferlin","sequence":"additional","affiliation":[{"name":"Unit of Andrology and Reproductive Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1053\/j.gastro.2014.03.001","article-title":"The Intestinal Metabolome: An Intersection between Microbiota and Host","volume":"146","author":"Ursell","year":"2014","journal-title":"Gastroenterology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1146\/annurev-genom-090711-163814","article-title":"The Human Microbiome: Our Second Genome","volume":"13","author":"Grice","year":"2012","journal-title":"Annu. Rev. Genom. Hum. Genet."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Berg, G., Rybakova, D., Fischer, D., Cernava, T., Verg\u00e8s, M.-C.C., Charles, T., Chen, X., Cocolin, L., Eversole, K., and Corral, G.H. (2020). Microbiome Definition Re-Visited: Old Concepts and New Challenges. Microbiome, 8.","DOI":"10.1186\/s40168-020-00875-0"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5","DOI":"10.15171\/mejdd.2020.157","article-title":"Signature of Gut Microbiome by Conventional and Advanced Analysis Techniques: Advantages and Disadvantages","volume":"12","author":"Rezasoltani","year":"2019","journal-title":"Middle East J. Dig. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1097\/MOG.0000000000000139","article-title":"The Gut Microbiome in Health and in Disease","volume":"31","author":"Shreiner","year":"2015","journal-title":"Curr. Opin. Gastroenterol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1042\/BCJ20160510","article-title":"Introduction to the human gut microbiota","volume":"474","author":"Thursby","year":"2017","journal-title":"Biochem. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"103114","DOI":"10.1016\/j.jaut.2023.103114","article-title":"The gut ecosystem and immune tolerance","volume":"141","author":"Shao","year":"2023","journal-title":"J. Autoimmun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2959","DOI":"10.1007\/s00018-017-2509-x","article-title":"Mechanisms and Consequences of Intestinal Dysbiosis","volume":"74","author":"Weiss","year":"2017","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1136\/postgradmedj-2021-141311","article-title":"Gut microbiota and obesity: An overview of microbiota to microbial-based therapies","volume":"99","author":"Sheykhsaran","year":"2023","journal-title":"Postgrad. Med. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s12610-016-0034-7","article-title":"Gut Endotoxin Leading to a Decline IN Gonadal Function (GELDING)\u2014A Novel Theory for the Development of Late Onset Hypogonadism in Obese Men","volume":"26","author":"Tremellen","year":"2016","journal-title":"Basic Clin. Androl."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0739-7240(03)00042-0","article-title":"Endotoxin Inhibition of Luteinizing Hormone in Sheep","volume":"25","author":"Daniel","year":"2003","journal-title":"Domest. Anim. Endocrinol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2827","DOI":"10.1210\/en.2011-0031","article-title":"Toll-like receptor-initiated testicular innate immune responses in mouse Leydig cells","volume":"52","author":"Shang","year":"2011","journal-title":"Endocrinology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.reprotox.2006.03.003","article-title":"Bacterial lipopolysaccharide-induced oxidative stress in the impairment of steroidogenesis and spermatogenesis in rats","volume":"22","author":"Reddy","year":"2006","journal-title":"Reprod. Toxicol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0165-0378(02)00020-7","article-title":"Testicular macrophage modulation of Leydig cell steroidogenesis","volume":"57","author":"Hales","year":"2002","journal-title":"J. Reprod. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Shen, P., Ji, S., Li, X., Yang, Q., Xu, B., Wong, C.K.C., Wang, L., and Li, L. (2022). LPS-Induced Systemic Inflammation Caused mPOA-FSH\/LH Disturbance and Impaired Testicular Function. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.886085"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4260","DOI":"10.1038\/srep04260","article-title":"Interleukin-6 disrupts blood-testis barrier through inhibiting protein degradation or activating phosphorylated ERK in Sertoli cells","volume":"4","author":"Zhang","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Cao, T., Wang, S., Pan, Y., Guo, F., Wu, B., Zhang, Y., Wang, Y., Tian, J., Xing, Q., and Liu, X. (2023). Characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1182320"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1016\/j.fertnstert.2007.08.083","article-title":"Detection and Identification of Bacterial DNA in Semen","volume":"90","author":"Kiessling","year":"2008","journal-title":"Fertil. Steril."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/j.eururo.2021.01.014","article-title":"Functional and Taxonomic Dysbiosis of the Gut, Urine, and Semen Microbiomes in Male Infertility","volume":"79","author":"Lundy","year":"2021","journal-title":"Eur. Urol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.rbmo.2021.06.021","article-title":"Assessing the Testicular Sperm Microbiome: A Low-Biomass Site with Abundant Contamination","volume":"43","author":"Molina","year":"2021","journal-title":"Reprod. Biomed. Online"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"110417","DOI":"10.1016\/j.forsciint.2020.110417","article-title":"Effect of Indoor Environmental Exposure on Seminal Microbiota and Its Application in Body Fluid Identification","volume":"314","author":"Yao","year":"2020","journal-title":"Forensic Sci. Int."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"\u0160t\u0161epetova, J., Baranova, J., Simm, J., Parm, \u00dc., R\u00f6\u00f6p, T., Sokmann, S., Korrovits, P., Jaagura, M., Rosenstein, K., and Salumets, A. (2020). The Complex Microbiome from Native Semen to Embryo Culture Environment in Human In Vitro Fertilization Procedure. Reprod. Biol. Endocrinol., 18.","DOI":"10.1186\/s12958-019-0562-z"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Campisciano, G., Iebba, V., Zito, G., Luppi, S., Martinelli, M., Fischer, L., De Seta, F., Basile, G., Ricci, G., and Comar, M. (2020). Lactobacillus Iners and Gasseri, Prevotella Bivia and HPV Belong to the Microbiological Signature Negatively Affecting Human Reproduction. Microorganisms, 9.","DOI":"10.3390\/microorganisms9010039"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Weng, S.-L., Chiu, C.-M., Lin, F.-M., Huang, W.-C., Liang, C., Yang, T., Yang, T.-L., Liu, C.-Y., Wu, W.-Y., and Chang, Y.-A. (2014). Bacterial Communities in Semen from Men of Infertile Couples: Metagenomic Sequencing Reveals Relationships of Seminal Microbiota to Semen Quality. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0110152"},{"key":"ref_25","first-page":"2884","article-title":"Seminal Bacterial Composition in Patients with Obstructive and Non-obstructive Azoospermia","volume":"15","author":"Chen","year":"2018","journal-title":"Exp. Ther. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1038\/s41585-019-0250-y","article-title":"The Seminal Microbiome in Health and Disease","volume":"16","author":"Franasiak","year":"2019","journal-title":"Nat. Rev. Urol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Garcia-Segura, S., del Rey, J., Closa, L., Garcia-Mart\u00ednez, I., Hobeich, C., Castel, A.B., Vidal, F., Benet, J., and Oliver-Bonet, M. (2023). Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing Platform. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24097867"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Opazo, M.C., Ortega-Rocha, E.M., Coronado-Arr\u00e1zola, I., Bonifaz, L.C., Boudin, H., Neunlist, M., Bueno, S.M., Kalergis, A.M., and Riedel, C.A. (2018). Intestinal Microbiota Influences Non-Intestinal Related Autoimmune Diseases. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.00432"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1038\/cmi.2014.41","article-title":"Innate and Adaptive Immune Responses in Male and Female Reproductive Tracts in Homeostasis and following HIV Infection","volume":"11","author":"Nguyen","year":"2014","journal-title":"Cell. Mol. Immunol."},{"key":"ref_30","unstructured":"(2024, May 01). WHO, Infertility. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/infertility."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1007\/s40618-022-01741-6","article-title":"Management of Male Factor Infertility: Position Statement from the Italian Society of Andrology and Sexual Medicine (SIAMS)","volume":"45","author":"Ferlin","year":"2022","journal-title":"J. Endocrinol. Investig."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"670","DOI":"10.12688\/f1000research.17076.1","article-title":"Recent Advances in Understanding and Managing Male Infertility","volume":"8","author":"Fainberg","year":"2019","journal-title":"F1000Research"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Ferlin, A., and Foresta, C. (2020). Infertility: Practical Clinical Issues for Routine Investigation of the Male Partner. J. Clin. Med., 9.","DOI":"10.3390\/jcm9061644"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Graziani, A., Rocca, M.S., Vinanzi, C., Masi, G., Grande, G., De Toni, L., and Ferlin, A. (2024). Genetic Causes of Qualitative Sperm Defects: A Narrative Review of Clinical Evidence. Genes, 15.","DOI":"10.3390\/genes15050600"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1080\/14737159.2019.1633917","article-title":"Contemporary Genetics-Based Diagnostics of Male Infertility","volume":"19","author":"Ferlin","year":"2019","journal-title":"Expert Rev. Mol. Diagn."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1093\/humrep\/deae032","article-title":"Guideline for Unexplained Couple Infertility: Misunderstandings on the Approach to the Male Factor","volume":"39","author":"Grande","year":"2024","journal-title":"Hum. Reprod."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"16993","DOI":"10.1038\/s41598-022-20574-0","article-title":"Enterotypes in Asthenospermia Patients with Obesity","volume":"12","author":"Jiao","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1038\/s41598-024-51686-4","article-title":"Semen Microbiota Are Dramatically Altered in Men with Abnormal Sperm Parameters","volume":"14","author":"Osadchiy","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1151S","DOI":"10.1093\/ajcn\/76.5.1151S","article-title":"S-Adenosyl-L-Methionine (SAMe): From the Bench to the Bedside--Molecular Basis of a Pleiotrophic Molecule","volume":"76","author":"Bottiglieri","year":"2002","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1093\/molehr\/gam093","article-title":"Abnormal Methylation of Imprinted Genes in Human Sperm Is Associated with Oligozoospermia","volume":"14","author":"Marques","year":"2008","journal-title":"MHR Basic. Sci. Reprod. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1093\/oxfordjournals.humrep.a137909","article-title":"Andrology: Placebo-Controlled, Double-Blind, Cross-over Trial of Glutathione Therapy in Male Infertility","volume":"8","author":"Lenzi","year":"1993","journal-title":"Hum. Reprod."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Engel, K.M., Baumann, S., Rolle-Kampczyk, U., Schiller, J., von Bergen, M., and Grunewald, S. (2019). Metabolomic Profiling Reveals Correlations between Spermiogram Parameters and the Metabolites Present in Human Spermatozoa and Seminal Plasma. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0211679"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Chen, P., Li, Y., Zhu, X., Ma, M., Chen, H., He, J., Liang, X., Liu, G., and Yang, X. (2023). Interaction between Host and Microbes in the Semen of Patients with Idiopathic Nonobstructive Azoospermia. Microbiol. Spectr., 11.","DOI":"10.1128\/spectrum.04365-22"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1001\/jama.282.3.236","article-title":"NIH Consensus Definition and Classification of Prostatitis","volume":"282","author":"Krieger","year":"1999","journal-title":"JAMA J. Am. Med. Assoc."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.eururo.2015.08.061","article-title":"Contemporary Management of Chronic Prostatitis\/Chronic Pelvic Pain Syndrome","volume":"69","author":"Magistro","year":"2016","journal-title":"Eur. Urol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Graziani, A., Grande, G., Martin, M., Ferraioli, G., Colonnello, E., Iafrate, M., Dal Moro, F., and Ferlin, A. (2023). Chronic Prostatitis\/Chronic Pain Pelvic Syndrome and Male Infertility. Life, 13.","DOI":"10.3390\/life13081700"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e12992","DOI":"10.1111\/aji.12992","article-title":"Identification of Seminal Markers of Male Accessory Gland Inflammation: From Molecules to Proteome","volume":"80","author":"Grande","year":"2018","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/iju.13286","article-title":"Seminal Microbiome in Men with and without Prostatitis","volume":"24","author":"Punab","year":"2017","journal-title":"Int. J. Urol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"398","DOI":"10.4103\/aja202172","article-title":"Semen Microbiota in Normal and Leukocytospermic Males","volume":"24","author":"Yao","year":"2022","journal-title":"Asian J. Androl."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"155798832211190","DOI":"10.1177\/15579883221119064","article-title":"Association of Probiotic Treatment with Antibiotics in Male Accessory Gland Infections","volume":"16","author":"Grande","year":"2022","journal-title":"Am. J. Mens. Health"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"G1","DOI":"10.1016\/j.vaccine.2013.10.002","article-title":"Comprehensive Control of Human Papillomavirus Infections and Related Diseases","volume":"31","author":"Bosch","year":"2013","journal-title":"Vaccine"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s40618-023-02192-3","article-title":"HPV-Related Diseases in Male Patients: An Underestimated Conundrum","volume":"47","author":"Garolla","year":"2023","journal-title":"J. Endocrinol. Investig."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"G\u0142owienka-Stodolak, M., Bagi\u0144ska-Drabiuk, K., Szubert, S., Hennig, E.E., Horala, A., D\u0105browska, M., Micek, M., Ciebiera, M., and Zeber-Lubecka, N. (2024). Human Papillomavirus Infections and the Role Played by Cervical and Cervico-Vaginal Microbiota\u2014Evidence from Next-Generation Sequencing Studies. Cancers, 16.","DOI":"10.3390\/cancers16020399"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Tuominen, H., Rautava, J., Kero, K., Syrj\u00e4nen, S., Collado, M.C., and Rautava, S. (2021). HPV Infection and Bacterial Microbiota in the Semen from Healthy Men. BMC Infect. Dis., 21.","DOI":"10.1186\/s12879-021-06029-3"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.imlet.2014.05.014","article-title":"Fusobacterium and Enterobacteriaceae: Important Players for CRC?","volume":"162","author":"Jobin","year":"2014","journal-title":"Immunol. Lett."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Elnaggar, J.H., Huynh, V.O., Lin, D., Hillman, R.T., Abana, C.O., El Alam, M.B., Tomasic, K.C., Karpinets, T.V., Kouzy, R., and Phan, J.L. (2023). HPV-Related Anal Cancer Is Associated with Changes in the Anorectal Microbiome during Cancer Development. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1051431"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1973","DOI":"10.1136\/gutjnl-2015-310101","article-title":"Fusobacterium nucleatum in Colorectal Carcinoma Tissue and Patient Prognosis","volume":"65","author":"Mima","year":"2016","journal-title":"Gut"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1126\/science.aal5240","article-title":"Analysis of Fusobacterium Persistence and Antibiotic Response in Colorectal Cancer","volume":"358","author":"Bullman","year":"2017","journal-title":"Science"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Wang, S., Liu, Y., Li, J., Zhao, L., Yan, W., Lin, B., Guo, X., and Wei, Y. (2021). Fusobacterium nucleatum Acts as a Pro-Carcinogenic Bacterium in Colorectal Cancer: From Association to Causality. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.710165"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2907","DOI":"10.1128\/IAI.68.5.2907-2915.2000","article-title":"Inducible Expression of Human \u03b2-Defensin 2 by Fusobacterium nucleatum in Oral Epithelial Cells: Multiple Signaling Pathways and Role of Commensal Bacteria in Innate Immunity and the Epithelial Barrier","volume":"68","author":"Krisanaprakornkit","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1111\/j.1600-065X.2012.01099.x","article-title":"NF-\u03baB and the Link between Inflammation and Cancer","volume":"246","author":"DiDonato","year":"2012","journal-title":"Immunol. Rev."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.immuni.2015.01.010","article-title":"Binding of the Fap2 Protein of Fusobacterium nucleatum to Human Inhibitory Receptor TIGIT Protects Tumors from Immune Cell Attack","volume":"42","author":"Gur","year":"2015","journal-title":"Immunity"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1038\/nri3175","article-title":"Coordinated Regulation of Myeloid Cells by Tumours","volume":"12","author":"Gabrilovich","year":"2012","journal-title":"Nat. Rev. Immunol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"e969","DOI":"10.1016\/S2666-5247(22)00203-8","article-title":"The Gut Mycobiome in Health, Disease, and Clinical Applications in Association with the Gut Bacterial Microbiome Assembly","volume":"3","author":"Zhang","year":"2022","journal-title":"Lancet Microbe"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Hoffmann, C., Dollive, S., Grunberg, S., Chen, J., Li, H., Wu, G.D., Lewis, J.D., and Bushman, F.D. (2013). Archaea and Fungi of the Human Gut Microbiome: Correlations with Diet and Bacterial Residents. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0066019"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Petre, G.C., Francini-Pesenti, F., Di Nisio, A., De Toni, L., Grande, G., Mingardi, A., Cusmano, A., Spinella, P., Ferlin, A., and Garolla, A. (2023). Observational Cross-Sectional Study on Mediterranean Diet and Sperm Parameters. Nutrients, 15.","DOI":"10.3390\/nu15234989"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Fan, J., Zhou, Y., Meng, R., Tang, J., Zhu, J., Aldrich, M.C., Cox, N.J., Zhu, Y., Li, Y., and Zhou, D. (2023). Cross-Talks between Gut Microbiota and Tobacco Smoking: A Two-Sample Mendelian Randomization Study. BMC Med., 21.","DOI":"10.1186\/s12916-023-02863-1"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.fertnstert.2018.06.016","article-title":"Smoking and Infertility: A Committee Opinion","volume":"110","author":"Penzias","year":"2018","journal-title":"Fertil. Steril."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1016\/j.fertnstert.2013.07.1991","article-title":"Microbiota of the Seminal Fluid from Healthy and Infertile Men","volume":"100","author":"Hou","year":"2013","journal-title":"Fertil. Steril."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.phrs.2012.10.019","article-title":"Microbiota of Male Genital Tract: Impact on the Health of Man and His Partner","volume":"69","year":"2013","journal-title":"Pharmacol. Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.anaerobe.2011.04.015","article-title":"Influence of Sexual Intercourse on Genital Tract Microbiota in Infertile Couples","volume":"17","author":"Borovkova","year":"2011","journal-title":"Anaerobe"},{"key":"ref_72","first-page":"3","article-title":"Role of Escherichia coli Pili Adhesion Molecule to Inhibit Escherichia coli Adhesion to Human Spermatozoa In vitro","volume":"1","author":"Sukarjati","year":"2014","journal-title":"Androl. Gynecol. Curr. Res."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Zhang, F., Dai, J., and Chen, T. (2021). Role of Lactobacillus in Female Infertility Via Modulating Sperm Agglutination and Immobilization. Front. Cell Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.620529"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Teveroni, E., Di Nicuolo, F., Vergani, E., Bruno, C., Maulucci, G., Bianchetti, G., Astorri, A.L., Grande, G., Gervasoni, J., and Santucci, L. (2022). Short-Chain Fatty Acids Modulate Sperm Migration through Olfactory Receptor 51E2 Activity. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232112726"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1111\/aogs.14598","article-title":"Lactobacillus crispatus-dominated Vaginal Microbiome and Acinetobacter-dominated Seminal Microbiome Support Beneficial art Outcome","volume":"102","author":"Koort","year":"2023","journal-title":"Acta Obstet. Gynecol. Scand."},{"key":"ref_76","first-page":"341","article-title":"Probiotics supplementation in the treatment of male infertility: A Systematic Review","volume":"28","author":"Brasil","year":"2024","journal-title":"JBRA Assist. Reprod."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1017\/S1751731112001528","article-title":"Antioxidative activity and protective effect of probiotics against high-fat diet-induced sperm damage in rats","volume":"7","author":"Chen","year":"2013","journal-title":"Animal"},{"key":"ref_78","first-page":"235","article-title":"Synbiotic (FamiLact) administration in idiopathic male infertility enhances sperm quality, DNA integrity, and chromatin status: A triple-blinded randomized clinical trial","volume":"19","author":"Abbasi","year":"2021","journal-title":"Int. J. Reprod. Biomed."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1111\/andr.12336","article-title":"The association of a probiotic with a prebiotic (Flortec, Bracco) to improve the quality\/quantity of spermatozoa in infertile patients with idiopathic oligoasthenoteratospermia: A pilot study","volume":"5","author":"Maretti","year":"2017","journal-title":"Andrology"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/13\/15\/1275\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:26:01Z","timestamp":1760109961000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/13\/15\/1275"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,29]]},"references-count":79,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["cells13151275"],"URL":"https:\/\/doi.org\/10.3390\/cells13151275","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,29]]}}}