{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:18:39Z","timestamp":1760145519232,"version":"build-2065373602"},"reference-count":132,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T00:00:00Z","timestamp":1723161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation (DFG)","doi-asserted-by":"publisher","award":["ME 1706\/18-1"],"award-info":[{"award-number":["ME 1706\/18-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Sonnenfeld-Stiftung (Berlin, Germany)","award":["ME 1706\/18-1"],"award-info":[{"award-number":["ME 1706\/18-1"]}]},{"name":"Open Access Publication Fund of Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin","award":["ME 1706\/18-1"],"award-info":[{"award-number":["ME 1706\/18-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The conjunctiva has immune-responsive properties to protect the eye from infections. Its innate immune system reacts against external pathogens, such as fungi. The complement factor C5a is an important contributor to the initial immune response. It is known that activation of transient-receptor-potential-vanilloid 1 (TRPV1) and TRP-melastatin 8 (TRPM8) channels is involved in different immune reactions and inflammation in the human body. The aim of this study was to determine if C5a and mucor\u00a0racemosus e voluminae cellulae (MR) modulate Ca2+-signaling through changes in TRPs activity in human conjunctival epithelial cells (HCjECs). Furthermore, crosstalk was examined between C5a and MR in mediating calcium regulation. Intracellular Ca2+-concentration ([Ca2+]i) was measured by fluorescence calcium imaging, and whole-cell currents were recorded using the planar-patch-clamp technique. MR was used as a purified extract. Application of C5a (0.05\u201350 ng\/mL) increased both [Ca2+]i and whole-cell currents, which were suppressed by either the TRPV1-blocker AMG 9810 or the TRPM8-blocker AMTB (both 20 \u00b5M). The N-terminal peptide C5L2p (20\u201350 ng\/mL) blocked rises in [Ca2+]i induced by C5a. Moreover, the MR-induced rise in Ca2+-influx was suppressed by AMG 9810 and AMTB, as well as 0.05 ng\/mL C5a. In conclusion, crosstalk between C5a and MR controls human conjunctival cell function through modulating interactions between TRPV1 and TRPM8 channel activity.<\/jats:p>","DOI":"10.3390\/cells13161329","type":"journal-article","created":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T05:19:38Z","timestamp":1723439978000},"page":"1329","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Complement Component C5a and Fungal Pathogen Induce Diverse Responses through Crosstalk between Transient Receptor Potential Channel (TRPs) Subtypes in Human Conjunctival Epithelial Cells"],"prefix":"10.3390","volume":"13","author":[{"given":"Loreena","family":"Rech","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, 10117 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9041-2249","authenticated-orcid":false,"given":"Tina","family":"Dietrich-Ntoukas","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, 10117 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3494-4523","authenticated-orcid":false,"given":"Peter S.","family":"Reinach","sequence":"additional","affiliation":[{"name":"School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou 325015, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6939-1159","authenticated-orcid":false,"given":"Tobias","family":"Brockmann","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Universit\u00e4tsmedizin Rostock, 18057 Rostock, Germany"},{"name":"SciTec Department, University of Applied Sciences Jena, 07745 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5569-1473","authenticated-orcid":false,"given":"Uwe","family":"Pleyer","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, 10117 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8608-648X","authenticated-orcid":false,"given":"Stefan","family":"Mergler","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, 10117 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,9]]},"reference":[{"key":"ref_1","first-page":"363","article-title":"Histological, immunohistochemical and clinical considerations on amniotic membrane transplant for ocular surface reconstruction","volume":"58","author":"Costea","year":"2017","journal-title":"Rom. 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