{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:19:53Z","timestamp":1760145593130,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T00:00:00Z","timestamp":1723507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Grants in Aid for Scientific Research","award":["24K11740","12103677"],"award-info":[{"award-number":["24K11740","12103677"]}]},{"name":"START Project of the Ministry of Education, Culture, Sports, Science and Technology","award":["24K11740","12103677"],"award-info":[{"award-number":["24K11740","12103677"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Tongue squamous cell carcinoma (TSCC) occurs frequently in the oral cavity, and because of its high proliferative and metastatic potential, it is necessary to develop a novel treatment for it. We have reported the importance of the inhibition of the periostin (POSTN) pathological splicing variant, including exon 21 (PN1-2), in various malignancies, but its influence is unclear in tongue cancer. In this study, we investigated the potential of POSTN exon 21-specific neutralizing antibody (PN21-Ab) as a novel treatment for TSCC. Human PN2 was transfected into the human TSCC (HSC-3) and cultured under stress, and PN2 was found to increase cell viability. PN2 induced chemotherapy resistance in HSC-3 via the phosphorylation of the cell survival signal Akt. In tissues from human TSCC and primary tumors of an HSC-3 xenograft model, PN1-2 was expressed in the tumor stroma, mainly from fibroblasts. The intensity of PN1-2 mRNA expression was positively correlated with malignancy. In the HSC-3 xenograft model, CDDP and PN21-Ab promoted CDPP\u2019s inhibition of tumor growth. These results suggest that POSTN exon 21 may be a biomarker for tongue cancer and that PN21-Ab may be a novel treatment for chemotherapy-resistant tongue cancer. The treatment points towards important innovations for TSCC, but many more studies are needed to extrapolate the results.<\/jats:p>","DOI":"10.3390\/cells13161341","type":"journal-article","created":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T10:54:54Z","timestamp":1723546494000},"page":"1341","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Experimental Study: The Development of a Novel Treatment for Chemotherapy-Resistant Tongue Cancer with the Inhibition of the Pathological Periostin Splicing Variant 1-2 with Exon 21"],"prefix":"10.3390","volume":"13","author":[{"given":"Shoji","family":"Ikebe","sequence":"first","affiliation":[{"name":"Graduate School of Dentistry (Second Department of Oral and Maxillofacial Surgery), Osaka Dental University, Hirakata 573-1121, Japan"},{"name":"Department of Advanced Molecular Therapy, Graduate School of Medicine, Faculty of Medicine, Osaka University, Suita 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobutaka","family":"Koibuchi","sequence":"additional","affiliation":[{"name":"Department of Advanced Molecular Therapy, Graduate School of Medicine, Faculty of Medicine, Osaka University, Suita 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kana","family":"Shibata","sequence":"additional","affiliation":[{"name":"Department of Advanced Molecular Therapy, Graduate School of Medicine, Faculty of Medicine, Osaka University, Suita 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3728-5607","authenticated-orcid":false,"given":"Fumihiro","family":"Sanada","sequence":"additional","affiliation":[{"name":"Department of Clinical Gene Therapy, Graduate School of Medicine, Faculty of Medicine, Osaka University, Suita 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Osaka Dental University, Hirakata 573-1121, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiko","family":"Takenobu","sequence":"additional","affiliation":[{"name":"Second Department of Oral and Maxillofacial Surgery, Osaka Dental University, Hirakata 573-1121, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiaki","family":"Taniyama","sequence":"additional","affiliation":[{"name":"Department of Advanced Molecular Therapy, Graduate School of Medicine, Faculty of Medicine, Osaka University, Suita 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S1535-6108(04)00090-X","article-title":"Focus on head and neck cancer","volume":"5","author":"Mao","year":"2004","journal-title":"Cancer Cell"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Lu, Y., Zheng, Z., Yuan, Y., Pathak, J.L., Yang, X., Wang, L., Ye, Z., Cho, W.C., Zeng, M., and Wu, L. 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