{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:29:52Z","timestamp":1777526992870,"version":"3.51.4"},"reference-count":35,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T00:00:00Z","timestamp":1724630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordination of Improvement of Higher Education Personnel","award":["n\u00ba 88881.311044\/2018-00"],"award-info":[{"award-number":["n\u00ba 88881.311044\/2018-00"]}]},{"name":"Coordination of Improvement of Higher Education Personnel","award":["2018-13588-0"],"award-info":[{"award-number":["2018-13588-0"]}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo State Research Support Foundation","doi-asserted-by":"publisher","award":["n\u00ba 88881.311044\/2018-00"],"award-info":[{"award-number":["n\u00ba 88881.311044\/2018-00"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo State Research Support Foundation","doi-asserted-by":"publisher","award":["2018-13588-0"],"award-info":[{"award-number":["2018-13588-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Butantan Foundation","award":["n\u00ba 88881.311044\/2018-00"],"award-info":[{"award-number":["n\u00ba 88881.311044\/2018-00"]}]},{"name":"Butantan Foundation","award":["2018-13588-0"],"award-info":[{"award-number":["2018-13588-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The Lucena 1 cell line, derived from the human chronic myeloid leukemia cell line K562 under selective pressure of vincristine supplementation, exhibits multidrug resistance (MDR). This study aims to explore and elucidate the underlying mechanisms driving MDR in the Lucena 1 cell line. A proteomic analysis comparing K562 and Lucena 1 revealed qualitative differences, with a focus on the ATP-dependent efflux pump, Translocase ABCB1, a key contributor to drug resistance. Tubulin analysis identified two unique isoforms, Tubulin beta 8B and alpha chain-like 3, exclusive to Lucena 1, potentially influencing resistance mechanisms. Additionally, the association of Rap1A and Krit1 in cytoskeletal regulation and the presence of STAT1, linked to the urea cycle and tumor development, offered insights into Lucena 1\u2019s distinctive biology. The increased expression of carbonic anhydrase I suggested a role in pH regulation. The discovery of COP9, a tumor suppressor targeting p53, further highlighted the Lucena 1 complex molecular landscape. This study offers new insights into the MDR phenotype and its multifactorial consequences in cellular pathways. Thus, unraveling the mechanisms of MDR holds promise for innovating cancer models and antitumor targeted strategies, since inhibiting the P-glycoprotein (P-gp)\/ABCB1 protein is not always an effective approach given the associated treatment toxicity.<\/jats:p>","DOI":"10.3390\/cells13171427","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T07:53:37Z","timestamp":1724658817000},"page":"1427","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Proteomic Dynamics of Multidrug Resistance Mechanisms in Lucena 1 Cell Line"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4735-5162","authenticated-orcid":false,"given":"Emidio","family":"Beraldo-Neto","sequence":"first","affiliation":[{"name":"Biochemistry Laboratory, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"},{"name":"Department of Biophysics, Paulista School of Medicine, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04023-062, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2405-887X","authenticated-orcid":false,"given":"Fernanda Cardoso","family":"Amador","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Paulista School of Medicine, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04023-062, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karolina Rosa","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Paulista School of Medicine, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04023-062, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giselle Zenker","family":"Justo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Paulista School of Medicine, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04023-062, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4588-7197","authenticated-orcid":false,"given":"Jos\u00e9 Thalles","family":"Lacerda","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Paulista School of Medicine, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04023-062, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria A.","family":"Juliano","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Paulista School of Medicine, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04023-062, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1182\/blood.2022015850","article-title":"International Consensus Classification of Myeloid Neoplasms and Acute Leukemias: Integrating Morphologic, Clinical, and Genomic Data","volume":"140","author":"Arber","year":"2022","journal-title":"Blood"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/s00428-022-03430-4","article-title":"The International Consensus Classification of Acute Myeloid Leukemia","volume":"482","author":"Weinberg","year":"2023","journal-title":"Virchows Arch."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1182\/blood.2022016867","article-title":"Diagnosis and Management of AML in Adults: 2022 Recommendations from an International Expert Panel on Behalf of the ELN","volume":"140","author":"Wei","year":"2022","journal-title":"Blood"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1001\/jama.2022.14578","article-title":"Diagnosis and Treatment of Myelodysplastic Syndromes: A Review","volume":"328","author":"Sekeres","year":"2022","journal-title":"JAMA"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2794","DOI":"10.3324\/haematol.2022.281493","article-title":"Measurable Residual Disease in Chronic Myeloid Leukemia","volume":"107","author":"Branford","year":"2022","journal-title":"Haematologica"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1478-811X-11-33","article-title":"Targeting Self-Renewal Pathways in Myeloid Malignancies","volume":"11","author":"Sands","year":"2013","journal-title":"Cell Commun. 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