{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T09:44:59Z","timestamp":1775468699757,"version":"3.50.1"},"reference-count":55,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T00:00:00Z","timestamp":1725408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Project of China","award":["2018YFA0902804"],"award-info":[{"award-number":["2018YFA0902804"]}]},{"name":"National Key Research and Development Project of China","award":["32471003"],"award-info":[{"award-number":["32471003"]}]},{"name":"National Key Research and Development Project of China","award":["31870861"],"award-info":[{"award-number":["31870861"]}]},{"name":"National Natural Science Foundation","award":["2018YFA0902804"],"award-info":[{"award-number":["2018YFA0902804"]}]},{"name":"National Natural Science Foundation","award":["32471003"],"award-info":[{"award-number":["32471003"]}]},{"name":"National Natural Science Foundation","award":["31870861"],"award-info":[{"award-number":["31870861"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The complex structure of monoclonal antibodies (mAbs) expressed in Chinese hamster ovary (CHO) cells may result in the accumulation of unfolded proteins, triggering endoplasmic reticulum (ER) stress and an unfolded protein response (UPR). If the protein folding ability cannot maintain ER homeostasis, the cell will shut down protein translation and ultimately induce apoptosis. We co-overexpressed HsQSOX1b and survivin proteins in the antibody-producing cell line CHO-PAb to obtain a new cell line, CHO-PAb-QS. Compared with CHO-PAb cells, the survival time of CHO-PAb-QS cells in batch culture was extended by 2 days, and the antibody accumulation and productivity were increased by 52% and 45%, respectively. The proportion of (HC-LC)2 was approximately doubled in the CHO-PAb-QS cells, which adapted to the accelerated disulfide bond folding capacity by upregulating the UPR\u2019s strength and increasing the ER content. The results of the apoptosis assays indicated that the CHO-PAb-QS cell line exhibited more excellent resistance to apoptosis induced by ER stress. Finally, CHO-PAb-QS cells exhibited mild oxidative stress but did not significantly alter the redox status. This study demonstrated that strategies based on HsQSOX1b and survivin co-overexpression could facilitate protein disulfide bond folding and anti-apoptosis ability, enhancing antibody production efficiency in CHO cell lines.<\/jats:p>","DOI":"10.3390\/cells13171481","type":"journal-article","created":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T02:25:49Z","timestamp":1725416749000},"page":"1481","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Enhancing the Antibody Production Efficiency of Chinese Hamster Ovary Cells through Improvement of Disulfide Bond Folding Ability and Apoptosis Resistance"],"prefix":"10.3390","volume":"13","author":[{"given":"Chen","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunhui","family":"Fu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenyun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Chang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinping","family":"Niu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangmin","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8740-7748","authenticated-orcid":false,"given":"Xingyuan","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.copbio.2019.01.011","article-title":"The fickle CHO: A review of the causes, implications, and potential alleviation of the CHO cell line instability problem","volume":"60","author":"Dahodwala","year":"2019","journal-title":"Curr. Opin. Biotech."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ddtec.2021.02.003","article-title":"Recent advances in CHO cell line development for recombinant protein production","volume":"38","author":"Tihanyi","year":"2020","journal-title":"Drug Discov. Today Technol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Hamdi, A., Sz\u00e9liov\u00e1, D., Ruckerbauer, D.E., Rocha, I., Borth, N., and Zanghellini, J. (2020). Key Challenges in Designing CHO Chassis Platforms. Processes, 8.","DOI":"10.3390\/pr8060643"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Zheng, M., Zhen, L., Xiao, Y., and Tian, Y. (2022). Factors affecting the expression of recombinant protein and improvement strategies in Chinese hamster ovary cells. Front. Bioeng. Biotech., 10.","DOI":"10.3389\/fbioe.2022.880155"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1023\/A:1008077603328","article-title":"Quantitative analysis of transcription and translation in gene amplified Chinese hamster ovary cells on the basis of a kinetic model","volume":"29","author":"Friedl","year":"1999","journal-title":"Cytotechnology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.jbiosc.2018.06.018","article-title":"Analysis of intracellular IgG secretion in Chinese hamster ovary cells to improve IgG production","volume":"127","author":"Kaneyoshi","year":"2019","journal-title":"J. Biosci. Bioeng."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s10616-013-9678-8","article-title":"Dynamics of unfolded protein response in recombinant CHO cells","volume":"67","author":"Prashad","year":"2015","journal-title":"Cytotechnology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.ymben.2022.02.002","article-title":"Endogenous BiP reporter system for simultaneous identification of ER stress and antibody production in Chinese hamster ovary cells","volume":"72","author":"Kyeong","year":"2022","journal-title":"Metab. Eng."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/ni1149","article-title":"Building an antibody factory: A job for the unfolded protein response","volume":"6","author":"Brewer","year":"2005","journal-title":"Nat. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1002\/bit.20861","article-title":"Engineering responsiveness to cell culture stresses: Growth arrest and DNA damage gene 153 (GADD153) and the unfolded protein response (UPR) in NS0 myeloma cells","volume":"94","author":"Cudna","year":"2006","journal-title":"Biotechnol. Bioeng."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1002\/bit.20403","article-title":"eIF2\u03b1 phosphorylation, stress perception, and the shutdown of global protein synthesis in cultured CHO cells","volume":"89","author":"Underhill","year":"2005","journal-title":"Biotechnol. Bioeng."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1002\/bit.26263","article-title":"ATF6\u03b2-based fine-tuning of the unfolded protein response enhances therapeutic antibody productivity of Chinese hamster ovary cells","volume":"114","author":"Pieper","year":"2017","journal-title":"Biotechnol. Bioeng."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"447","DOI":"10.2174\/138920108786786367","article-title":"Development and production of commercial therapeutic monoclonal antibodies in Mammalian cell expression systems: An overview of the current upstream technologies","volume":"9","author":"Chartrain","year":"2008","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_14","first-page":"309","article-title":"The immunoglobulin fold. Structural classification, sequence patterns and common core","volume":"242","author":"Bork","year":"1994","journal-title":"J. Mol. Biol."},{"key":"ref_15","first-page":"63","article-title":"IgG Fc glycosylation in human immunity","volume":"423","author":"Wang","year":"2019","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"17","DOI":"10.4161\/mabs.4.1.18347","article-title":"Disulfide bond structures of IgG molecules: Structural variations, chemical modifications and possible impacts to stability and biological function","volume":"4","author":"Liu","year":"2012","journal-title":"mAbs"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Benham, A., Lith, M., Sitia, R., and Braakman, I. (2013). Ero1-PDI interactions, the response to redox flux and the implications for disulfide bond formation in the mammalian endoplasmic reticulum. Philos. Trans. R. Soc. London. Ser. B Biol. Sci., 368.","DOI":"10.1098\/rstb.2011.0403"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.jbiosc.2020.08.001","article-title":"Effect of the disulfide isomerase PDIa4 on the antibody production of Chinese hamster ovary cells","volume":"130","author":"Komatsu","year":"2020","journal-title":"J. Biosci. Bioeng."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1021\/bp0498241","article-title":"Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line","volume":"21","author":"Borth","year":"2005","journal-title":"Biotechnol. Progr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1021\/bp000107q","article-title":"Effect of PDI overexpression on recombinant protein secretion in CHO cells","volume":"16","author":"Davis","year":"2000","journal-title":"Biotechnol. Progr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1002\/bit.21453","article-title":"Effect of doxycycline-regulated protein disulfide isomerase expression on the specific productivity of recombinant CHO cells: Thrombopoietin and antibody","volume":"98","author":"Mohan","year":"2007","journal-title":"Biotechnol. Bioeng."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S1097-2765(00)80017-9","article-title":"The ERO1 gene of yeast is required for oxidation of protein dithiols in the endoplasmic reticulum","volume":"1","author":"Frand","year":"1998","journal-title":"Mol. Cell"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1002\/bit.22781","article-title":"Effect of inducible co-overexpression of protein disulfide isomerase and endoplasmic reticulum oxidoreductase on the specific antibody productivity of recombinant Chinese hamster ovary cells","volume":"107","author":"Mohan","year":"2010","journal-title":"Biotechnol. Bioeng."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1089\/ars.2014.5851","article-title":"Endoplasmic reticulum stress and oxidative stress in cell fate decision and human disease","volume":"21","author":"Cao","year":"2014","journal-title":"Antioxid. Redox Signal."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1002\/bit.27269","article-title":"Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production","volume":"117","author":"Henry","year":"2020","journal-title":"Biotechnol. Bioeng."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"7602","DOI":"10.1128\/MCB.20.20.7602-7612.2000","article-title":"Inhibition of Daxx-mediated apoptosis by heat shock protein 27","volume":"20","author":"Charette","year":"2000","journal-title":"Mol. Cell Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"195","DOI":"10.3727\/000000001783992605","article-title":"Hsp27 inhibits cytochrome c-mediated caspase activation by sequestering both pro-caspase-3 and cytochrome c","volume":"9","author":"Concannon","year":"2001","journal-title":"Gene Expr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1002\/biot.201400764","article-title":"Heat shock protein 27 overexpression in CHO cells modulates apoptosis pathways and delays activation of caspases to improve recombinant monoclonal antibody titre in fed-batch bioreactors","volume":"10","author":"Tan","year":"2015","journal-title":"Biotechnol. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1080\/07388551.2016.1214557","article-title":"Gene editing for cell engineering: Trends and applications","volume":"37","author":"Gupta","year":"2017","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1007\/s10529-018-2578-4","article-title":"Stable expression of infliximab in CRISPR\/Cas9-mediated BAK1-deficient CHO cells","volume":"40","author":"Miao","year":"2018","journal-title":"Biotechnol. Lett."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1002\/bit.22541","article-title":"BAK and BAX deletion using zinc-finger nucleases yields apoptosis-resistant CHO cells","volume":"105","author":"Cost","year":"2010","journal-title":"Biotechnol. Bioeng."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1002\/btpr.192","article-title":"Mcl-1 overexpression leads to higher viabilities and increased production of humanized monoclonal antibody in Chinese hamster ovary cells","volume":"25","author":"Majors","year":"2009","journal-title":"Biotechnol Prog"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.ymben.2006.05.005","article-title":"RNAi suppression of Bax and Bak enhances viability in fed-batch cultures of CHO cells","volume":"8","author":"Lim","year":"2006","journal-title":"Metab. Eng."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.pharmthera.2012.02.003","article-title":"Stress management at the ER: Regulators of ER stress-induced apoptosis","volume":"134","author":"Gorman","year":"2012","journal-title":"Pharmacol. Ther."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1002\/bit.27548","article-title":"Omics driven discoveries of gene targets for apoptosis attenuation in CHO cells","volume":"118","author":"Orellana","year":"2021","journal-title":"Biotechnol. Bioeng."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.ymben.2007.06.001","article-title":"Specific inhibition of caspase-8 and -9 in CHO cells enhances cell viability in batch and fed-batch cultures","volume":"9","author":"Yun","year":"2007","journal-title":"Metab. Eng."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1089\/ars.2010.3098","article-title":"Oxidative protein folding and the quiescin-sulfhydryl oxidase family of flavoproteins","volume":"13","author":"Kodali","year":"2010","journal-title":"Antioxid. Redox Signal."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5341","DOI":"10.1111\/febs.13079","article-title":"Disulfide bond formation activity of soybean quiescin sulfhydryl oxidase","volume":"281","author":"Okuda","year":"2014","journal-title":"FEBS J."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Zheng, W., Zhang, W., Hu, W., Zhang, C., and Yang, Y. (2012). Exploring the Smallest Active Fragment of HsQSOX1b and Finding a Highly Efficient Oxidative Engine. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0040935"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.3892\/ol.2016.4867","article-title":"Survivin and gliomas: A literature review","volume":"12","author":"Rosilin","year":"2016","journal-title":"Oncol. Lett."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"16515","DOI":"10.1021\/acs.jmedchem.3c01130","article-title":"Small molecule inhibitors targeting the \u201cundruggable\" survivin: The Past, Present, and future from a medicinal chemist\u2019s perspective","volume":"66","author":"Cui","year":"2023","journal-title":"J. Med. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1002\/path.1300","article-title":"Apoptosis and melanoma: Molecular mechanisms","volume":"199","author":"Hussein","year":"2003","journal-title":"J. Pathol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1021\/acssynbio.7b00375","article-title":"Enhanced biosynthesis performance of heterologous proteins in CHO-K1 cells using CRISPR-Cas9","volume":"7","author":"Wang","year":"2018","journal-title":"ACS Synth. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Zhang, C., Chang, F., Miao, H., Fu, Y., Tong, X., Feng, Y., Zheng, W., and Ma, X. (2023). Construction and application of a multifunctional CHO cell platform utilizing Cre\/ and Dre\/ site-specific recombination systems. Front. Bioeng. Biotech., 11.","DOI":"10.3389\/fbioe.2023.1320841"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3211","DOI":"10.1021\/bi9820816","article-title":"Egg white sulfhydryl oxidase: Kinetic mechanism of the catalysis of disulfide bond formation","volume":"38","author":"Hoober","year":"1999","journal-title":"Biochemistry"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1038\/ncb1001-874","article-title":"A flavoprotein oxidase defines a new endoplasmic reticulum pathway for biosynthetic disulphide bond formation","volume":"3","author":"Sevier","year":"2001","journal-title":"Nat. Cell Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.ymben.2019.11.007","article-title":"Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production","volume":"57","author":"Budge","year":"2020","journal-title":"Metab. Eng."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.ymben.2021.12.004","article-title":"Reprogramming of Chinese hamster ovary cells towards enhanced protein secretion","volume":"69","author":"Torresa","year":"2022","journal-title":"Metab. Eng."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"389","DOI":"10.4103\/0971-5916.159250","article-title":"Survivin: A molecular biomarker in cancer","volume":"141","author":"Jaiswal","year":"2015","journal-title":"Indian J. Med. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"314","DOI":"10.7150\/jca.13332","article-title":"Survivin and Tumorigenesis: Molecular Mechanisms and Therapeutic Strategies","volume":"7","author":"Xun","year":"2016","journal-title":"J. Cancer"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1002\/bit.27247","article-title":"Oxidative stress-alleviating strategies to improve recombinant protein production in CHO cells","volume":"117","author":"Chevallier","year":"2020","journal-title":"Biotechnol. Bioeng."},{"key":"ref_52","first-page":"1","article-title":"Screening of dietary antioxidants against mitochondria-mediated oxidative stress by visualization of intracellular redox state","volume":"80","author":"Sunita","year":"2016","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1002\/btpr.2047","article-title":"Factors that determine stability of highly concentrated chemically defined production media","volume":"31","author":"Mccoy","year":"2015","journal-title":"Biotechnol. Prog."},{"key":"ref_54","first-page":"99","article-title":"Cell culture, oxidative stress, and antioxidants: Avoiding pitfalls","volume":"37","author":"Barry","year":"2014","journal-title":"Biomed. J."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Ha, T.K., Hansen, A.H., Kol, S., Kildegaard, H.F., and Lee, G.M. (2017). Baicalein Reduces Oxidative Stress in CHO Cell Cultures and Improves Recombinant Antibody Productivity. Biotechnol. 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