{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T10:06:35Z","timestamp":1772532395883,"version":"3.50.1"},"reference-count":73,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T00:00:00Z","timestamp":1728432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Missouri Department of Health and Senior Services","award":["#AOC23380006"],"award-info":[{"award-number":["#AOC23380006"]}]},{"name":"Children\u2019s Mercy-Kansas City","award":["#AOC23380006"],"award-info":[{"award-number":["#AOC23380006"]}]},{"name":"Katharine B. Richardson Associates Endowment Fund","award":["#AOC23380006"],"award-info":[{"award-number":["#AOC23380006"]}]},{"name":"Patton Trust Grant (Kansas City Area Life Sciences)","award":["#AOC23380006"],"award-info":[{"award-number":["#AOC23380006"]}]},{"name":"Kiersznowski Family Charitable Trust","award":["#AOC23380006"],"award-info":[{"award-number":["#AOC23380006"]}]},{"name":"KCVA Medical Center","award":["#AOC23380006"],"award-info":[{"award-number":["#AOC23380006"]}]},{"name":"MVBRF","award":["#AOC23380006"],"award-info":[{"award-number":["#AOC23380006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Chronic kidney disease (CKD) is a leading cause of morbidity and mortality globally. Maternal obesity during pregnancy is linked to systemic inflammation and elevated levels of the pro-inflammatory cytokine interleukin-6 (IL-6). In our previous work, we demonstrated that increased maternal IL-6 during gestation impacts intrauterine development in mice. We hypothesized that IL-6-induced inflammation alters gene expression in the developing fetus. To test this, pregnant mice were administered IL-6 or saline during mid-gestation. Newborn mouse kidneys were analyzed using mRNA-seq, miRNA-seq and whole-genome bisulfite-seq (WGBS). A multi-omics approach was employed to quantify mRNA gene expression, miRNA expression and DNA methylation, using advanced bioinformatics and data integration techniques. Our analysis identified 19 key genes present in multiple omics datasets, regulated by epigenetics and miRNAs. We constructed a regulatory network for these genes, revealing disruptions in pathways such as Mannose type O-glycan biosynthesis, the cell cycle, apoptosis and FoxO signaling. Notably, the Atp7b gene was regulated by DNA methylation and miR-223 targeting, whereas the Man2a1 gene was controlled by DNA methylation affecting energy metabolism. These findings suggest that these genes may play a role in fetal programming, potentially leading to CKD later in life due to gestational inflammation.<\/jats:p>","DOI":"10.3390\/cells13191667","type":"journal-article","created":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T06:30:03Z","timestamp":1728455403000},"page":"1667","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Employing Multi-Omics Analyses to Understand Changes during Kidney Development in Perinatal Interleukin-6 Animal Model"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3940-6724","authenticated-orcid":false,"given":"Ganesh","family":"Panzade","sequence":"first","affiliation":[{"name":"Christopher S. Bond Life Sciences Center, University of Missouri-Columbia, Columbia, MO 65211, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8654-0627","authenticated-orcid":false,"given":"Tarak","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Section of Nephrology, Children\u2019s Mercy Hospital and University of Missouri at Kansas City, 2401 Gillham Road, Kansas City, MO 64108, USA"},{"name":"Midwest Veterans\u2019 Biomedical Research Foundation (MVBRF), Kansas City, MO 64128, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3170-2585","authenticated-orcid":false,"given":"Daniel P.","family":"Heruth","sequence":"additional","affiliation":[{"name":"Children\u2019s Mercy Research Institute, Children\u2019s Mercy Hospital and University of Missouri at Kansas City, Kansas City, MO 64108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad H.","family":"Rezaiekhaligh","sequence":"additional","affiliation":[{"name":"Section of Nephrology, Children\u2019s Mercy Hospital and University of Missouri at Kansas City, 2401 Gillham Road, Kansas City, MO 64108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianping","family":"Zhou","sequence":"additional","affiliation":[{"name":"Midwest Veterans\u2019 Biomedical Research Foundation (MVBRF), Kansas City, MO 64128, USA"},{"name":"Kansas City VA Medical Center, Kansas City, MO 64128, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Lyu","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering and Computer Science, University of Missouri-Columbia, Columbia, MO 65211, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mukut","family":"Sharma","sequence":"additional","affiliation":[{"name":"Midwest Veterans\u2019 Biomedical Research Foundation (MVBRF), Kansas City, MO 64128, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8944-4924","authenticated-orcid":false,"given":"Trupti","family":"Joshi","sequence":"additional","affiliation":[{"name":"Christopher S. Bond Life Sciences Center, University of Missouri-Columbia, Columbia, MO 65211, USA"},{"name":"Department of Electrical Engineering and Computer Science, University of Missouri-Columbia, Columbia, MO 65211, USA"},{"name":"Department of Biomedical Informatics, Biostatistics and Medical Epidemiology, University of Missouri-Columbia, Columbia, MO 65211, USA"},{"name":"MU Institute for Data Science and Informatics, University of Missouri-Columbia, Columbia, MO 65211, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1111\/j.1523-1755.2004.00582.x","article-title":"Body Mass Index and the Risk of Development of End-Stage Renal Disease in a Screened Cohort","volume":"65","author":"Iseki","year":"2004","journal-title":"Kidney Int."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1097\/00000441-200209000-00003","article-title":"Mechanisms of Obesity-Associated Cardiovascular and Renal Disease","volume":"324","author":"Hall","year":"2002","journal-title":"Am. 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