{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:19:45Z","timestamp":1790882385196,"version":"4.1.0"},"reference-count":32,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>We assessed the anti-inflammatory activity of the TSPO ligand 2-Cl-MGV-1. Lipopolysaccharide (LPS) was used to induce inflammatory response in a murine RAW264.7 macrophage model (LPS: 100 ng\/mL) and a mouse model (C57BL\/6) of lung inflammation (LPS: 5 mg\/kg). In the macrophage model, the presence of 2-Cl-MGV-1 (25 \u00b5M) caused the LPS-induced elevation in nitrite levels to decrease by 70% (p &lt; 0.0001) and interleukin (IL)-6 by 50% (p &lt; 0.05). In the mouse model, 2-Cl-MGV-1, administered 30 min before, or co-administered with, an LPS injection, significantly inhibited the elevation in serum IL-5 levels (both by 65%; p &lt; 0.001 and p &lt; 0.01, respectively). 2-Cl-MGV-1 administration to mice 30 min before LPS injection and 1 h thereafter significantly inhibited the elevation in IL-1\u03b2 serum levels (both by 63%, p &lt; 0.005). IL-6 elevation was inhibited by 73% (p &lt; 0.005) when 2-Cl-MGV-1 was administered 30 min before LPS, by 60% (p &lt; 0.05) when co-administered with LPS, and by 64% (p &lt; 0.05) when administered 1 h after LPS. All cytokine assessments were conducted 6 h post LPS injection. Histological analyses showed decreased leukocyte adherence in the lung tissue of the ligand-treated mice. 2-Cl-MGV-1 administration 30 min prior to exposure to LPS inhibited inflammation-induced open field immobility. The beneficial effect of 2-Cl-MGV-1 suggests its potential as a therapeutic option for inflammatory diseases.<\/jats:p>","DOI":"10.3390\/cells13201702","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T08:09:08Z","timestamp":1728979748000},"page":"1702","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Novel TSPO Ligand 2-Cl-MGV-1 Can Counteract Lipopolysaccharide Induced Inflammatory Response in Murine RAW264.7 Macrophage Cell Line and Lung Models"],"prefix":"10.3390","volume":"13","author":[{"given":"Fadi","family":"Obeid","sequence":"first","affiliation":[{"name":"Ruth and Bruce Rappaport Faculty of Medicine, Technion\u2014Israel Institute of Technology, Haifa 31096, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meygal","family":"Kahana","sequence":"additional","affiliation":[{"name":"Ruth and Bruce Rappaport Faculty of Medicine, Technion\u2014Israel Institute of Technology, Haifa 31096, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baraah","family":"Dahle","sequence":"additional","affiliation":[{"name":"Ruth and Bruce Rappaport Faculty of Medicine, Technion\u2014Israel Institute of Technology, Haifa 31096, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7326-6083","authenticated-orcid":false,"given":"Sheelu","family":"Monga","sequence":"additional","affiliation":[{"name":"Ruth and Bruce Rappaport Faculty of Medicine, Technion\u2014Israel Institute of Technology, Haifa 31096, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaniv","family":"Zohar","sequence":"additional","affiliation":[{"name":"Ruth and Bruce Rappaport Faculty of Medicine, Technion\u2014Israel Institute of Technology, Haifa 31096, Israel"},{"name":"Institute of Pathology and Cytology, Rambam Health Care Campus, P.O. Box 9602, Haifa 3109601, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9765-8938","authenticated-orcid":false,"given":"Abraham","family":"Weizman","sequence":"additional","affiliation":[{"name":"Laboratory of Biological and Molecular Psychiatry, Felsenstein Medical Research Center, Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel"},{"name":"Research Unit, Geha Mental Health Center, Petah Tikva 4910002, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moshe","family":"Gavish","sequence":"additional","affiliation":[{"name":"Ruth and Bruce Rappaport Faculty of Medicine, Technion\u2014Israel Institute of Technology, Haifa 31096, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"ref_1","first-page":"169","article-title":"Chapter 9\u2014The impact of systemic inflammation on neuroinflammation","volume":"Volume 7","author":"Rezaei","year":"2023","journal-title":"Translational Neuroimmunology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1016\/j.jhep.2020.11.048","article-title":"The systemic inflammation hypothesis: Towards a new paradigm of acute decompensation and multiorgan failure in cirrhosis","volume":"74","author":"Arroyo","year":"2021","journal-title":"J. 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