{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:17:16Z","timestamp":1788826636337,"version":"build-2803163510"},"reference-count":44,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T00:00:00Z","timestamp":1729728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research Deanship fund, University of Ha\u2019il, Saudi Arabia","award":["RG-23067"],"award-info":[{"award-number":["RG-23067"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Vincamine, a monoterpenoid indole alkaloid with vasodilatory properties, is extracted from the leaves of Vinca minor. The present study aimed to determine the potential anticancer effects of vincamine loaded in silver nanoparticles (VCN-AgNPs) in mice with Ehrlich solid carcinoma (ESC). After tumor transplantation, the mice were divided into five groups: ESC, ESC+Cisplatin (CPN; 5 mg\/kg), ESC+VCN (40 mg\/kg), ESC+AgNPs (6 mg\/kg), and ESC+VCN-AgNPs (20 mg\/kg). The administration of VCN-AgNPs to ESC-bearing mice improved their survival rate and reduced their body weight, tumor size, and tumor weight compared to the ESC group. Furthermore, VCN-AgNPs intensified oxidative stress in tumor tissues, as evidenced by elevated levels of lipid peroxidation (LPO) and nitric oxide (NO), along with a reduction in the levels of the antioxidants investigated (GSH, GPx, GR, SOD, CAT, and TAC). Furthermore, VCN-AgNPs increased the apoptotic proteins Bax and caspase-3, decreased the anti-apoptotic protein (Bcl-2), increased the inflammatory markers TNF-\u03b1 and IL-1\u03b2, and inhibited angiogenesis by lowering VEGF levels in tumor tissues, all of which led to apoptosis. Furthermore, histopathological studies showed that VCN-AgNPs suppressed the progression of Ehrlich carcinoma and induced the formation of clusters of necrotic and fragmented tumor cells. VCN-AgNPs possess cytotoxic and genotoxic effects against ESC because of their pro-oxidant, pro-apoptotic, pro-inflammatory, and antiangiogenic effects. Additionally, the combination of VCN-AgNPs was more effective and safer than chemically synthesized AgNPs, as indicated by an increase in the lifespan of animals and the total tumor inhibition index.<\/jats:p>","DOI":"10.3390\/cells13211762","type":"journal-article","created":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T09:11:47Z","timestamp":1729761107000},"page":"1762","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of Vincamine Loaded with Silver Nanoparticles as a New Potential Therapeutic Agent Against Ehrlich\u2019s Solid Carcinoma in Mice"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3560-8166","authenticated-orcid":false,"given":"Naief","family":"Dahran","sequence":"first","affiliation":[{"name":"Department of Basic Medical Sciences, College of Medicine, University of Jeddah, Jeddah 21959, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0316-0961","authenticated-orcid":false,"given":"Mohamed S.","family":"Othman","sequence":"additional","affiliation":[{"name":"Biochemistry Department, College of Medicine, University of Ha\u2019il, Ha\u2032il 2440, Saudi Arabia"},{"name":"Faculty of Biotechnology, October University for Modern Science and Arts (MSA), Giza 12566, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3957-4365","authenticated-orcid":false,"given":"Mohamed E.","family":"Ghoniem","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, University of Ha\u2019il, Ha\u2019il 2240, Saudi Arabia"},{"name":"Department of Internal Medicine, Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5837-3921","authenticated-orcid":false,"given":"Mai A.","family":"Samak","sequence":"additional","affiliation":[{"name":"College of Medicine, University of Ha\u2019il, Ha\u2019il 2240, Saudi Arabia"},{"name":"Department of Medical Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0138-825X","authenticated-orcid":false,"given":"Mohamed T.","family":"Elabbasy","sequence":"additional","affiliation":[{"name":"College of Medicine, University of Ha\u2019il, Ha\u2019il 2240, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3096-9464","authenticated-orcid":false,"given":"Sofian T.","family":"Obeidat","sequence":"additional","affiliation":[{"name":"Basic Sciences Department, Deanship of Preparatory Year, University of Ha\u2019il, Ha\u2032il 2440, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghada M.","family":"Aleid","sequence":"additional","affiliation":[{"name":"Biochemistry Department, College of Medicine, University of Ha\u2019il, Ha\u2032il 2440, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimaa","family":"Abo Elnaga","sequence":"additional","affiliation":[{"name":"Basic Sciences Department, Deanship of Preparatory Year, University of Ha\u2019il, Ha\u2032il 2440, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azza M.","family":"Khaled","sequence":"additional","affiliation":[{"name":"Biochemistry Department, College of Medicine, University of Ha\u2019il, Ha\u2032il 2440, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya A.","family":"Altaleb","sequence":"additional","affiliation":[{"name":"College of Medicine, University of Gazi, Ankara 06500, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2654-2591","authenticated-orcid":false,"given":"Ahmed E.","family":"Abdel Moneim","sequence":"additional","affiliation":[{"name":"Zoology and Entomology Department, Faculty of Science, Helwan University, Ain Helwan, Cairo 11795, Egypt"},{"name":"Al-Ayen Scientific Research Center, Al-Ayen Iraqi University, AUIQ, An Nasiriyah, Thi Qar P.O. 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