{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:05:11Z","timestamp":1785935111711,"version":"3.56.0"},"reference-count":57,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T00:00:00Z","timestamp":1731369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>This prospective, case-control study evaluated the impact of obesity on oocyte quality based on mtDNA expression in cumulus cells (CC), and on bone morphogenetic protein 15 (BMP-15) and heparan sulfate proteoglycan 2 (HSPG2) in follicular fluid (FF). It included women 18 to &lt;40 years of age, divided according to BMI &lt; 24.9 (Group 1, n = 28) and BMI &gt; 25 (Group 2, n = 22). Demographics, treatment, and pregnancy outcomes were compared. The mtDNA in CC, BMP-15, HSPG2, the lipid profile, the hormonal profile, and C-reactive protein were evaluated in FF and in blood samples. The BMP-15 levels in FF and the mitochondrial DNA in CC were higher in Group 1 (38.8 \u00b1 32.5 vs. 14.3 \u00b1 10.8 ng\/mL; p = 0.001 and 1.10 \u00b1 0.3 vs. 0.87 \u00b1 0.18-fold change; p = 0.016, respectively) than in Group 2. High-density lipoprotein levels in blood and FF were higher in Group 1 (62 \u00b1 18 vs. 50 \u00b1 12 mg\/dL; p = 0.015 and 34 \u00b1 26 vs. 20.9 \u00b1 7.2 mg\/dL; p = 0.05, respectively). Group 2 had higher blood C-reactive protein (7.1 \u00b1 5.4 vs. 3.4 \u00b1 4.3 mg\/L; p = 0.015), FF (5.2 \u00b1 3.8 vs. 1.5 \u00b1 1.6 mg\/L; p = 0.002) and low-density lipoprotein levels (91 \u00b1 27 vs. 71 \u00b1 22 mg\/dL; p = 0.008) vs. Group 1. Group 1 demonstrated a trend toward a better clinical pregnancy rate (47.8% vs. 28.6%: p = 0.31) and frozen embryo transfer rate (69.2% vs. 53.8; p = 0.69). Higher BMI resulted in lower BMP-15 levels and reduced mtDNA expression, which reflect decreased oocyte quality in overweight women.<\/jats:p>","DOI":"10.3390\/cells13221872","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T05:21:59Z","timestamp":1731388919000},"page":"1872","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Excess Weight Impairs Oocyte Quality, as Reflected by mtDNA and BMP-15"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0205-123X","authenticated-orcid":false,"given":"Emiliya","family":"Sigal","sequence":"first","affiliation":[{"name":"IVF Unit, Department of Obstetrics and Gynecology, Hillel-Yaffe Medical Center, Hadera 3820302, Israel"},{"name":"A Ruth and Bruce Rappaport School of Medicine, Technion-Israel Institute of Technology, Haifa 3525433, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya","family":"Shavit","sequence":"additional","affiliation":[{"name":"IVF Unit, Department of Obstetrics and Gynecology, Hillel-Yaffe Medical Center, Hadera 3820302, Israel"},{"name":"A Ruth and Bruce Rappaport School of Medicine, Technion-Israel Institute of Technology, Haifa 3525433, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuval","family":"Atzmon","sequence":"additional","affiliation":[{"name":"IVF Unit, Department of Obstetrics and Gynecology, Hillel-Yaffe Medical Center, Hadera 3820302, Israel"},{"name":"A Ruth and Bruce Rappaport School of Medicine, Technion-Israel Institute of Technology, Haifa 3525433, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4807-9913","authenticated-orcid":false,"given":"Nardin","family":"Aslih","sequence":"additional","affiliation":[{"name":"IVF Unit, Department of Obstetrics and Gynecology, Hillel-Yaffe Medical Center, Hadera 3820302, Israel"},{"name":"A Ruth and Bruce Rappaport School of Medicine, Technion-Israel Institute of Technology, Haifa 3525433, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5440-7530","authenticated-orcid":false,"given":"Asaf","family":"Bilgory","sequence":"additional","affiliation":[{"name":"IVF Unit, Department of Obstetrics and Gynecology, Hillel-Yaffe Medical Center, Hadera 3820302, Israel"},{"name":"A Ruth and Bruce Rappaport School of Medicine, Technion-Israel Institute of Technology, Haifa 3525433, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniella","family":"Estrada","sequence":"additional","affiliation":[{"name":"IVF Unit, Department of Obstetrics and Gynecology, Hillel-Yaffe Medical Center, Hadera 3820302, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mediea","family":"Michaeli","sequence":"additional","affiliation":[{"name":"IVF Unit, Department of Obstetrics and Gynecology, Hillel-Yaffe Medical Center, Hadera 3820302, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nechama","family":"Rotfarb","sequence":"additional","affiliation":[{"name":"IVF Unit, Department of Obstetrics and Gynecology, Hillel-Yaffe Medical Center, Hadera 3820302, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmin","family":"Shibli Abu-Raya","sequence":"additional","affiliation":[{"name":"IVF Unit, Department of Obstetrics and Gynecology, Hillel-Yaffe Medical Center, Hadera 3820302, Israel"},{"name":"A Ruth and Bruce Rappaport School of Medicine, Technion-Israel Institute of Technology, Haifa 3525433, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilhav","family":"Meisel-Sharon","sequence":"additional","affiliation":[{"name":"IVF Unit, Department of Obstetrics and Gynecology, Hillel-Yaffe Medical Center, Hadera 3820302, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Einat","family":"Shalom-Paz","sequence":"additional","affiliation":[{"name":"IVF Unit, Department of Obstetrics and Gynecology, Hillel-Yaffe Medical Center, Hadera 3820302, Israel"},{"name":"A Ruth and Bruce Rappaport School of Medicine, Technion-Israel Institute of Technology, Haifa 3525433, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,12]]},"reference":[{"key":"ref_1","unstructured":"WHO (2000). 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