{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:06:17Z","timestamp":1769504777000,"version":"3.49.0"},"reference-count":37,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T00:00:00Z","timestamp":1731888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Aplastic anemia (AA) is characterized by bone marrow (BM) aplasia and pancytopenia. BM stromal microenvironment is closely intertwined with hematopoietic cells by reciprocal regulation. It is still unclear how hematopoietic deficiency affects the bone marrow stroma of the AA patients. Multipotent mesenchymal stromal cells (MMSCs) are the progenitors of stromal cells. In vitro, proliferation rate of MMSCs of AA patients is decreased compared to those of healthy donors. This may be explained by the influence of pathological environmental condition in the patients\u2019 BM. The aim of the study was to compare the effect of AA patients\u2019 sera on healthy donor MMSCs to healthy donors\u2019 sera and to elucidate the nature of their difference. Proliferation test showed 3-fold decrease in number of MMSCs after incubation in medium supplemented with AA patients\u2019 sera compared to donors\u2019 serum samples. The degree of this effect correlated with the severity of thrombocytopenia in patients. The decrease in cell number was not associated with cell death, as the number of apoptotic cells defined by flow cytometry did not differ between the groups. ELISA revealed a decreased level of PDGF-BB in the patients\u2019 sera compared to donors\u2019 serum samples (69 \u00b1 5 pg\/mL vs. 112 \u00b1 21 pg\/mL, respectively). The addition of recombinant PDGF-BB or healthy donor\u2019s platelet lysate to the culture medium supplemented with AA patients\u2019 serum restored its ability to support MMSCs growth. Thus, PDGF-BB deficiency is one of the environmental factors causing MMSCs damage in AA patients.<\/jats:p>","DOI":"10.3390\/cells13221908","type":"journal-article","created":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T13:03:14Z","timestamp":1731934994000},"page":"1908","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["PDGF-BB Deficiency in the Blood Serum from Aplastic Anemia Patients Affects Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells"],"prefix":"10.3390","volume":"13","author":[{"given":"Alena I.","family":"Dorofeeva","sequence":"first","affiliation":[{"name":"National Medical Research Center for Hematology, Moscow 127167, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1189-0283","authenticated-orcid":false,"given":"Irina N.","family":"Shipounova","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Hematology, Moscow 127167, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ksenia A.","family":"Nikiforova","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Hematology, Moscow 127167, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina V.","family":"Galtseva","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Hematology, Moscow 127167, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larisa A.","family":"Kuzmina","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Hematology, Moscow 127167, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anton V.","family":"Luchkin","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Hematology, Moscow 127167, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zalina T.","family":"Fidarova","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Hematology, Moscow 127167, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena A.","family":"Mikhailova","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Hematology, Moscow 127167, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena N.","family":"Parovichnikova","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Hematology, Moscow 127167, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s12185-017-2262-9","article-title":"Complexity of Bone Marrow Hematopoietic Stem Cell Niche","volume":"106","author":"Asada","year":"2017","journal-title":"Int. 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