{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:10:13Z","timestamp":1787242213272,"version":"build-2736575974"},"reference-count":57,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Simmaron Research Inc.","award":["R21NS129021-01A1"],"award-info":[{"award-number":["R21NS129021-01A1"]}]},{"name":"NIH R21 grant","award":["R21NS129021-01A1"],"award-info":[{"award-number":["R21NS129021-01A1"]}]},{"name":"Ramsay Award 2023","award":["R21NS129021-01A1"],"award-info":[{"award-number":["R21NS129021-01A1"]}]},{"name":"Foundation Hesse Sibylla, Quebec, QC, Canada","award":["R21NS129021-01A1"],"award-info":[{"award-number":["R21NS129021-01A1"]}]},{"name":"Therapeutic Acceleration Program (TAP) grant to MMH by the Medical College of Wisconsin, WI","award":["R21NS129021-01A1"],"award-info":[{"award-number":["R21NS129021-01A1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Enhancement of neuronal plasticity by small-molecule therapeutics protects cognitive skills and also ameliorates progressive neurodegenerative pathologies like Alzheimer\u2019s disease (AD) and dementia. One such compound, a novel histone deacetylase 2 (HDAC2) inhibitor named JRM-28, was shown here to enhance dendritic strength, augment spine density, and upregulate post-synaptic neurotransmission in hippocampal neurons. The molecular basis for this effect correlates with JRM-28-induced upregulation of the transcription of cAMP response element-binding protein(CREB), induction of its transcriptional activity, and subsequent stimulation of expressions of CREB-dependent plasticity-associated genes, such as those encoding N-methyl-D-aspartate (NMDA) receptor subunit NR2A and the \u03b1-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit GluR1. Specifically, JRM-28 stimulated the NMDA- and AMPA-receptor-sensitive ionotropic calcium influx in hippocampal neurons. Interestingly, JRM-28 did not induce NMDA- and AMPA-sensitive calcium influx in hippocampal neurons once the expression of CREB was knocked down by creb siRNA, suggesting the critical role of CREB in JRM-28-mediated upregulation of synaptic plasticity. Finally, JRM-28 upregulated CREB mRNA, CREB-dependent plasticity-associated markers, and ionotropic calcium influx in iPSC-derived AD human neurons, indicating its therapeutic implications in the amelioration of AD pathologies.<\/jats:p>","DOI":"10.3390\/cells13231964","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T03:21:23Z","timestamp":1732764083000},"page":"1964","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["JRM-28, a Novel HDAC2 Inhibitor, Upregulates Plasticity-Associated Proteins in Hippocampal Neurons and Enhances Morphological Plasticity via Activation of CREB: Implications for Alzheimer\u2019s Disease"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-8185-7687","authenticated-orcid":false,"given":"A. F. M. 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