{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T05:14:31Z","timestamp":1791695671579,"version":"4.3.4"},"reference-count":41,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health\u2014 Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)","award":["HD 049607"],"award-info":[{"award-number":["HD 049607"]}]},{"name":"National Institutes of Health\u2014 Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)","award":["#35203-16148"],"award-info":[{"award-number":["#35203-16148"]}]},{"name":"National Institutes of Health\u2014 Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)","award":["GR 696"],"award-info":[{"award-number":["GR 696"]}]},{"name":"National Institutes of Health\u2014 Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)","award":["DAAD19-03-1-0168"],"award-info":[{"award-number":["DAAD19-03-1-0168"]}]},{"name":"United States Department of Agriculture (USDA)-CSREES National Research Initiative Competitive Grants Program","award":["HD 049607"],"award-info":[{"award-number":["HD 049607"]}]},{"name":"United States Department of Agriculture (USDA)-CSREES National Research Initiative Competitive Grants Program","award":["#35203-16148"],"award-info":[{"award-number":["#35203-16148"]}]},{"name":"United States Department of Agriculture (USDA)-CSREES National Research Initiative Competitive Grants Program","award":["GR 696"],"award-info":[{"award-number":["GR 696"]}]},{"name":"United States Department of Agriculture (USDA)-CSREES National Research Initiative Competitive Grants Program","award":["DAAD19-03-1-0168"],"award-info":[{"award-number":["DAAD19-03-1-0168"]}]},{"name":"Michigan Economic Development Corporation","award":["HD 049607"],"award-info":[{"award-number":["HD 049607"]}]},{"name":"Michigan Economic Development Corporation","award":["#35203-16148"],"award-info":[{"award-number":["#35203-16148"]}]},{"name":"Michigan Economic Development Corporation","award":["GR 696"],"award-info":[{"award-number":["GR 696"]}]},{"name":"Michigan Economic Development Corporation","award":["DAAD19-03-1-0168"],"award-info":[{"award-number":["DAAD19-03-1-0168"]}]},{"name":"U.S. Army Research Laboratory and the U.S. Army Research Office","award":["HD 049607"],"award-info":[{"award-number":["HD 049607"]}]},{"name":"U.S. Army Research Laboratory and the U.S. Army Research Office","award":["#35203-16148"],"award-info":[{"award-number":["#35203-16148"]}]},{"name":"U.S. Army Research Laboratory and the U.S. Army Research Office","award":["GR 696"],"award-info":[{"award-number":["GR 696"]}]},{"name":"U.S. Army Research Laboratory and the U.S. Army Research Office","award":["DAAD19-03-1-0168"],"award-info":[{"award-number":["DAAD19-03-1-0168"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Classical preimplantation embryo culture is performed in static fluid environments. Whether a dynamic fluid environment, like the fallopian tube, is beneficial for embryo development remains to be determined across mammalian species. Objectives of these proof-of-concept studies were to determine if controllable dynamic microfluidic culture would enhance preimplantation murine, bovine, and human embryo development compared to static culture. This prospective randomized controlled trial tested static versus controlled dynamic culture of preimplantation mouse (n = 397), bovine (n = 242), and human (n = 512) zygotes to blastocyst stages with outcome measures of embryo cleavage, cellular fragmentation, apoptosis, and blastocyst conversion rates. Dynamic culture of mouse and bovine zygotes with microfluidics significantly improved embryo development. Mouse placental imprinted gene expression was significantly different between embryos derived in vivo, by static culture, and by dynamic culture. Using human sibling zygotes, this dynamic microfluidic culture system increased the number of blastomeres per cleavage-stage embryo, reduced cellular fragmentation or apoptosis, improved blastocyst conversion rates, and enhanced blastocyst developmental stages. In conclusion, species-specific longitudinal studies demonstrated that dynamic microfluidic culture significantly improved embryo development, independent of culture media composition, temperature, and gaseous environment. These cellular indicators represent improved embryo development that can translate into higher pregnancy rates in transgenics, domestic livestock and endangered species and treating human infertility.<\/jats:p>","DOI":"10.3390\/cells13242080","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T05:26:02Z","timestamp":1734413162000},"page":"2080","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Controlled Dynamic Microfluidic Culture of Murine, Bovine, and Human Embryos Improves Development: Proof-of-Concept Studies"],"prefix":"10.3390","volume":"13","author":[{"given":"Jose Roberto","family":"Alegretti","sequence":"first","affiliation":[{"name":"Huntington\u2014Medicina Reprodutiva, Av Republica do Libano, 529 Ibirapuera, Sao Paulo 04501-000, SP, Brazil"},{"name":"Department of Gynecology, Federal University of Sao Paulo-UNIFESP, Rua Napoleao de Barros, 715-7\u00b0, Sao Paulo 04024-002, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3324-3773","authenticated-orcid":false,"given":"Andre M. Da","family":"Rocha","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Michigan, 1500 E. Medical Center Dr., Ann Arbor, MI 48109-0617, USA"},{"name":"Internal Medicine\u2014Cardiology\/Frankel CVC Regeneration Core, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI 48109, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4486-181X","authenticated-orcid":false,"given":"Naiara C.","family":"Nogueira-de-Souza","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Michigan, 1500 E. Medical Center Dr., Ann Arbor, MI 48109-0617, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8210-6305","authenticated-orcid":false,"given":"Nobuhiro","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Michigan, 1500 E. Medical Center Dr., Ann Arbor, MI 48109-0617, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna C.","family":"Barros","sequence":"additional","affiliation":[{"name":"Huntington\u2014Medicina Reprodutiva, Av Republica do Libano, 529 Ibirapuera, Sao Paulo 04501-000, SP, Brazil"},{"name":"Department of Gynecology, Federal University of Sao Paulo-UNIFESP, Rua Napoleao de Barros, 715-7\u00b0, Sao Paulo 04024-002, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo L. A.","family":"Motta","sequence":"additional","affiliation":[{"name":"Huntington\u2014Medicina Reprodutiva, Av Republica do Libano, 529 Ibirapuera, Sao Paulo 04501-000, SP, Brazil"},{"name":"Department of Gynecology, Federal University of Sao Paulo-UNIFESP, Rua Napoleao de Barros, 715-7\u00b0, Sao Paulo 04024-002, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo C.","family":"Serafini","sequence":"additional","affiliation":[{"name":"Huntington\u2014Medicina Reprodutiva, Av Republica do Libano, 529 Ibirapuera, Sao Paulo 04501-000, SP, Brazil"},{"name":"Department of Gynecology, Federal University of Sao Paulo-UNIFESP, Rua Napoleao de Barros, 715-7\u00b0, Sao Paulo 04024-002, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4385-9080","authenticated-orcid":false,"given":"Shuichi","family":"Takayama","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel Blvd., Ann Arbor, MI 48109-0617, USA"},{"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, Atlanta, GA 30332, USA"},{"name":"The Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, 315 Ferst Dr., NW, Atlanta, GA 30332, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary D.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Michigan, 1500 E. Medical Center Dr., Ann Arbor, MI 48109-0617, USA"},{"name":"Department of Urology, University of Michigan, 1500 E. Medical Center Dr., Ann Arbor, MI 48109-0617, USA"},{"name":"Department of Physiology, University of Michigan, 1500 E. Medical Center Dr., Ann Arbor, MI 48109-0617, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1016\/S0015-0282(16)49834-1","article-title":"Factors influencing murine embryo bioassay: Effects of proteins, aging of medium, and surgical glove coatings","volume":"46","author":"Naz","year":"1986","journal-title":"Fertil. Steril."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1095\/biolreprod48.2.377","article-title":"Amino acids and ammonium regulate mouse embryo development in culture","volume":"48","author":"Gardner","year":"1993","journal-title":"Biol. 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