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Infiltration of surrounding normal tissues limits surgical approaches, wide inter- and intratumor heterogeneity hinders the development of universal therapeutics, and the presence of the blood\u2013brain barrier reduces the efficiency of their delivery. As a result, patients diagnosed with gliomas often face a poor prognosis and low survival rates. The spectrum of anti-glioma drugs used in clinical practice is quite narrow. Alkylating agents are often used as first-line therapy, but their effectiveness varies depending on the molecular subtypes of gliomas. This highlights the need for new, more effective therapeutic approaches. Standard drug-screening methods involve the use of two-dimensional cell cultures. However, these models cannot fully replicate the conditions present in real tumors, making it difficult to extrapolate the results to humans. We describe the advantages and disadvantages of existing glioma cell-based models designed to improve the situation and build future prospects to make drug discovery comprehensive and more effective for each patient according to personalized therapy paradigms.<\/jats:p>","DOI":"10.3390\/cells13242085","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T08:17:00Z","timestamp":1734423420000},"page":"2085","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Cell-Based Glioma Models for Anticancer Drug Screening: From Conventional Adherent Cell Cultures to Tumor-Specific Three-Dimensional Constructs"],"prefix":"10.3390","volume":"13","author":[{"given":"Daria","family":"Lanskikh","sequence":"first","affiliation":[{"name":"School of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3799-1691","authenticated-orcid":false,"given":"Olga","family":"Kuziakova","sequence":"additional","affiliation":[{"name":"School of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4632-4508","authenticated-orcid":false,"given":"Ivan","family":"Baklanov","sequence":"additional","affiliation":[{"name":"School of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2524-4015","authenticated-orcid":false,"given":"Alina","family":"Penkova","sequence":"additional","affiliation":[{"name":"School of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"Doroshenko","sequence":"additional","affiliation":[{"name":"School of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3485-8024","authenticated-orcid":false,"given":"Ivan","family":"Buriak","sequence":"additional","affiliation":[{"name":"School of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0655-8489","authenticated-orcid":false,"given":"Valeriia","family":"Zhmenia","sequence":"additional","affiliation":[{"name":"School of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1639-664X","authenticated-orcid":false,"given":"Vadim","family":"Kumeiko","sequence":"additional","affiliation":[{"name":"School of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1158\/2159-8290.CD-20-1474","article-title":"Diffuse Glioma Heterogeneity and Its Therapeutic Implications","volume":"11","author":"Nicholson","year":"2021","journal-title":"Cancer Discov."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/s41572-024-00516-y","article-title":"Glioma","volume":"10","author":"Weller","year":"2024","journal-title":"Nat. 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