{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T09:43:34Z","timestamp":1786787014665,"version":"build-2736575974"},"reference-count":137,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["R01HL157456"],"award-info":[{"award-number":["R01HL157456"]}]},{"name":"National Institutes of Health","award":["R01HL168464"],"award-info":[{"award-number":["R01HL168464"]}]},{"name":"National Institutes of Health","award":["R01HL167670"],"award-info":[{"award-number":["R01HL167670"]}]},{"name":"National Institutes of Health","award":["R01HL171495"],"award-info":[{"award-number":["R01HL171495"]}]},{"name":"National Institutes of Health","award":["968781"],"award-info":[{"award-number":["968781"]}]},{"DOI":"10.13039\/100000968","name":"American Heart Associate","doi-asserted-by":"publisher","award":["R01HL157456"],"award-info":[{"award-number":["R01HL157456"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Associate","doi-asserted-by":"publisher","award":["R01HL168464"],"award-info":[{"award-number":["R01HL168464"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Associate","doi-asserted-by":"publisher","award":["R01HL167670"],"award-info":[{"award-number":["R01HL167670"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Associate","doi-asserted-by":"publisher","award":["R01HL171495"],"award-info":[{"award-number":["R01HL171495"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Associate","doi-asserted-by":"publisher","award":["968781"],"award-info":[{"award-number":["968781"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Cardiovascular diseases resulting from myocardial infarction (MI) remain a leading cause of death worldwide, imposing a substantial burden on global health systems. Current MI treatments, primarily pharmacological and surgical, do not regenerate lost myocardium, leaving patients at high risk for heart failure. Engineered heart tissue (EHT) offers a promising solution for MI and related cardiac conditions by replenishing myocardial loss. However, challenges like immune rejection, inadequate vascularization, limited mechanical strength, and incomplete tissue maturation hinder clinical application. The discovery of human-induced pluripotent stem cells (hiPSCs) has transformed the EHT field, enabling new bioengineering innovations. This review explores recent advancements and future directions in hiPSC-derived EHTs, focusing on innovative materials and fabrication methods like bioprinting and decellularization, and assessing their therapeutic potential through preclinical and clinical studies. Achieving functional integration of EHTs in the heart remains challenging due to the need for synchronized contraction, sufficient vascularization, and mechanical compatibility. Solutions such as genome editing, personalized medicine, and AI technologies offer promising strategies to address these translational barriers. Beyond MI, EHTs also show potential in treating ischemic cardiomyopathy, heart valve engineering, and drug screening, underscoring their promise in cardiovascular regenerative medicine.<\/jats:p>","DOI":"10.3390\/cells13242098","type":"journal-article","created":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T08:56:32Z","timestamp":1734512192000},"page":"2098","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Current Advances and Future Directions of Pluripotent Stem Cells-Derived Engineered Heart Tissue for Treatment of Cardiovascular Diseases"],"prefix":"10.3390","volume":"13","author":[{"given":"Xingyu","family":"He","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Good","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1292-1143","authenticated-orcid":false,"given":"Wael","family":"Kalou","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3291-0629","authenticated-orcid":false,"given":"Waqas","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suchandrima","family":"Dutta","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5501-4992","authenticated-orcid":false,"given":"Sophie","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles Noah","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4895-2495","authenticated-orcid":false,"given":"Karthickeyan","family":"Chella Krishnan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Systems Physiology, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanbo","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialiang","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0872-1860","authenticated-orcid":false,"given":"Yigang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e93","DOI":"10.1161\/CIR.0000000000001123","article-title":"Heart disease and stroke statistics\u20142023 update: A report from the American Heart Association","volume":"147","author":"Tsao","year":"2023","journal-title":"Circulation"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"113934","DOI":"10.1016\/j.yexcr.2024.113934","article-title":"Direct differentiation of rat skin fibroblasts into cardiomyocytes","volume":"435","author":"Ahmad","year":"2024","journal-title":"Exp. Cell Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e021682","DOI":"10.1161\/JAHA.121.021682","article-title":"Trends in premature mortality from acute myocardial infarction in the United States, 1999 to 2019","volume":"11","author":"Dani","year":"2022","journal-title":"J. Am. Heart Assoc."},{"key":"ref_4","first-page":"16","article-title":"Myocardial Infarction","volume":"6","author":"Singh","year":"2021","journal-title":"Himal. J. Health Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"M\u0142ynarska, E., Czarnik, W., Fularski, P., Hajdys, J., Majchrowicz, G., Stabrawa, M., Rysz, J., and Franczyk, B. (2024). From Atherosclerotic Plaque to Myocardial Infarction\u2014The Leading Cause of Coronary Artery Occlusion. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25137295"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Lodrini, A.M., and Goumans, M.-J. (2021). Cardiomyocytes cellular phenotypes after myocardial infarction. Front. Cardiovasc. Med., 8.","DOI":"10.3389\/fcvm.2021.750510"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"120","DOI":"10.4070\/kcj.2019.0166","article-title":"Clopidogrel versus aspirin after dual antiplatelet therapy in acute myocardial infarction patients undergoing drug-eluting stenting","volume":"50","author":"Sim","year":"2020","journal-title":"Korean Circ. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2686","DOI":"10.1111\/bcp.15221","article-title":"Antiplatelet therapy in cardiovascular disease: Current status and future directions","volume":"88","author":"Passacquale","year":"2022","journal-title":"Br. J. Clin. Pharmacol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Heestermans, M., Poenou, G., Hamzeh-Cognasse, H., Cognasse, F., and Bertoletti, L. (2022). Anticoagulants: A short history, their mechanism of action, pharmacology, and indications. Cells, 11.","DOI":"10.3390\/cells11203214"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Wo\u0142owiec, \u0141., Grze\u015bk, G., Osiak, J., Wijata, A., M\u0119dlewska, M., Gaborek, P., Banach, J., Wo\u0142owiec, A., and G\u0142owacka, M. (2023). Beta-blockers in cardiac arrhythmias\u2013Clinical pharmacologist\u2019s point of view. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.1043714"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1002\/ejhf.2191","article-title":"Prospective ARNI vs. ACE inhibitor trial to DetermIne Superiority in reducing heart failure Events after Myocardial Infarction (PARADISE-MI): Design and baseline characteristics","volume":"23","author":"Jering","year":"2021","journal-title":"Eur. J. Heart Fail."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.ahj.2020.10.059","article-title":"Randomized evaluation of beta blocker and ACE-inhibitor\/angiotensin receptor blocker treatment in patients with myocardial infarction with non-obstructive coronary arteries (MINOCA-BAT): Rationale and design","volume":"231","author":"Agewall","year":"2021","journal-title":"Am. Heart J."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Mahmood, A., Eqan, M., Pervez, S., Javed, R., Ullah, R., Islam, A., Khan, A., Baig, A.A., Kumar, G., and Abaid-Ullah, M. (2021). Drugs Resistance in Heart Diseases. Biochemistry of Drug Resistance, Springer.","DOI":"10.1007\/978-3-030-76320-6_11"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Netala, V.R., Teertam, S.K., Li, H., and Zhang, Z. (2024). A Comprehensive Review of Cardiovascular Disease Management: Cardiac Biomarkers, Imaging Modalities, Pharmacotherapy, Surgical Interventions, and Herbal Remedies. Cells, 13.","DOI":"10.3390\/cells13171471"},{"key":"ref_15","unstructured":"Hardiman, S.C. (2023). Coronary Revascularization and Timing of Treatment: Comparative Effectiveness of PCI and CABG in British Columbia. [Ph.D. Thesis, University of British Columbia]."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3531","DOI":"10.1097\/MS9.0000000000000942","article-title":"Coronary allograft vasculopathy in post-heart transplant patients: Pathogenesis and role of cardiac computed tomography in diagnosis\u2014A comprehensive review","volume":"85","author":"Sharma","year":"2023","journal-title":"Ann. Med. Surg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"8815349","DOI":"10.1155\/2020\/8815349","article-title":"Pharmacological modulation of cardiac remodeling after myocardial infarction","volume":"2020","author":"Zhao","year":"2020","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.1002\/ehf2.12875","article-title":"Prioritizing symptom management in the treatment of chronic heart failure","volume":"7","author":"Koshy","year":"2020","journal-title":"ESC Heart Fail."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11886-021-01588-y","article-title":"Improving quality of life in heart failure","volume":"23","author":"Freedland","year":"2021","journal-title":"Curr. Cardiol. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Arjmand, B., Abedi, M., Arabi, M., Alavi-Moghadam, S., Rezaei-Tavirani, M., Hadavandkhani, M., Tayanloo-Beik, A., Kordi, R., Roudsari, P.P., and Larijani, B. (2021). Regenerative medicine for the treatment of ischemic heart disease; status and future perspectives. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.704903"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"633","DOI":"10.4252\/wjsc.v14.i8.633","article-title":"Combination of mesenchymal stem cells and three-dimensional collagen scaffold preserves ventricular remodeling in rat myocardial infarction model","volume":"14","author":"Khan","year":"2022","journal-title":"World J. Stem Cells"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1186\/s13287-021-02443-1","article-title":"Surfing the clinical trials of mesenchymal stem cell therapy in ischemic cardiomyopathy","volume":"12","author":"Matta","year":"2021","journal-title":"Stem Cell Res. Ther."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1161\/ATVBAHA.120.314695","article-title":"Clinical trial in a dish: Using patient-derived induced pluripotent stem cells to identify risks of drug-induced cardiotoxicity","volume":"41","author":"Lam","year":"2021","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1186\/s13287-021-02451-1","article-title":"Application of mesenchymal stem cell sheet to treatment of ischemic heart disease","volume":"12","author":"Chang","year":"2021","journal-title":"Stem Cell Res. Ther."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"19","DOI":"10.5334\/gh.1098","article-title":"Mesenchymal stem cell (MSCs) therapy for ischemic heart disease: A promising frontier","volume":"17","author":"Poomani","year":"2022","journal-title":"Glob. Heart"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Han, Y., Li, X., Zhang, Y., Han, Y., Chang, F., and Ding, J. (2019). Mesenchymal stem cells for regenerative medicine. Cells, 8.","DOI":"10.3390\/cells8080886"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Cai, L., Wang, R., and Zhang, D. (2023). Cardiac Disease Modeling with Engineered Heart Tissue. Handbook of Experimental Pharmacology, Springer.","DOI":"10.1007\/164_2023_681"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1161\/CIRCULATIONAHA.120.047904","article-title":"Human engineered heart tissue patches remuscularize the injured heart in a dose-dependent manner","volume":"143","author":"Querdel","year":"2021","journal-title":"Circulation"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Zou, J., Yang, W., Cui, W., Li, C., Ma, C., Ji, X., Hong, J., Qu, Z., Chen, J., and Liu, A. (2023). Therapeutic potential and mechanisms of mesenchymal stem cell-derived exosomes as bioactive materials in tendon\u2013bone healing. J. Nanobiotechnol., 21.","DOI":"10.1186\/s12951-023-01778-6"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Scafa Udri\u0219te, A., Niculescu, A.-G., Iliu\u021b\u0103, L., Bajeu, T., Georgescu, A., Grumezescu, A.M., and B\u0103dil\u0103, E. (2023). Progress in biomaterials for cardiac tissue engineering and regeneration. Polymers, 15.","DOI":"10.3390\/polym15051177"},{"key":"ref_31","unstructured":"Ciucci, G. (2021). Engineered Heart Tissues to Investigate the Role of Mechanical Loading and Injury in Cardiomyocyte Proliferation. [Ph.D. Thesis, University of Trento]."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Gokhan, I., Blum, T.S., and Campbell, S.G. (2024). Engineered heart tissue: Design considerations and the state of the art. Biophys. Rev., 5.","DOI":"10.1063\/5.0202724"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Pushp, P., and Gupta, M.K. (2021). Cardiac Tissue Engineering: Stem Cell Sources, Synthetic Biomaterials, and Scaffold Fabrication Methods. Engineering Materials for Stem Cell Regeneration, Springer.","DOI":"10.1007\/978-981-16-4420-7_11"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Massai, D., Pisani, G., Isu, G., Rodriguez Ruiz, A., Cerino, G., Galluzzi, R., Pisanu, A., Tonoli, A., Bignardi, C., and Audenino, A.L. (2020). Bioreactor platform for biomimetic culture and in situ monitoring of the mechanical response of in vitro engineered models of cardiac tissue. Front. Bioeng. Biotechnol., 8.","DOI":"10.3389\/fbioe.2020.00733"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/s00449-024-03004-5","article-title":"Analysis of the role of perfusion, mechanical, and electrical stimulation in bioreactors for cardiac tissue engineering","volume":"47","author":"Lara","year":"2024","journal-title":"Bioprocess Biosyst. Eng."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e144068","DOI":"10.1172\/jci.insight.144068","article-title":"In vivo grafting of large engineered heart tissue patches for cardiac repair","volume":"6","author":"Jabbour","year":"2021","journal-title":"JCI Insight"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Fassina, D., Costa, C.M., Bishop, M., Plank, G., Whitaker, J., Harding, S.E., and Niederer, S.A. (2023). Assessing the arrhythmogenic risk of engineered heart tissue patches through in silico application on infarcted ventricle models. Comput. Biol. Med., 154.","DOI":"10.1016\/j.compbiomed.2023.106550"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Pecha, S., and Yildirim, Y. (2017). Myocardial tissue engineering for cardiac repair. Liver, Lung and Heart Regeneration. Stem Cells in Clinical Applications, Springer.","DOI":"10.1007\/978-3-319-46693-4_9"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1007\/s00425-015-2362-9","article-title":"Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): A review","volume":"242","author":"Nguyen","year":"2015","journal-title":"Planta"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"I146","DOI":"10.1007\/s003950200043","article-title":"3D engineered heart tissue for replacement therapy","volume":"97","author":"Eschenhagen","year":"2002","journal-title":"Basic Res. Cardiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e99","DOI":"10.1038\/am.2014.19","article-title":"Hydrogels for cardiac tissue engineering","volume":"6","author":"Annabi","year":"2014","journal-title":"NPG Asia Mater."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.actbio.2019.05.016","article-title":"Engineered heart tissue models from hiPSC-derived cardiomyocytes and cardiac ECM for disease modeling and drug testing applications","volume":"92","author":"Goldfracht","year":"2019","journal-title":"Acta Biomater."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2798","DOI":"10.1016\/j.biomaterials.2013.12.052","article-title":"The effect of cyclic stretch on maturation and 3D tissue formation of human embryonic stem cell-derived cardiomyocytes","volume":"35","author":"Mihic","year":"2014","journal-title":"Biomaterials"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.biomaterials.2018.08.058","article-title":"Electrical stimulation increases hypertrophy and metabolic flux in tissue-engineered human skeletal muscle","volume":"198","author":"Khodabukus","year":"2019","journal-title":"Biomaterials"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Munawar, S., and Turnbull, I.C. (2021). Cardiac Tissue Engineering: Inclusion of Non-cardiomyocytes for Enhanced Features. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.653127"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1186\/s12967-016-1028-0","article-title":"Recent advances in bioprinting techniques: Approaches, applications and future prospects","volume":"14","author":"Li","year":"2016","journal-title":"J. Transl. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.addr.2015.11.020","article-title":"Fibrous scaffolds for building hearts and heart parts","volume":"96","author":"Capulli","year":"2016","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.actbio.2016.10.032","article-title":"Biodegradable and biomimetic elastomeric scaffolds for tissue-engineered heart valves","volume":"48","author":"Xue","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1126\/science.abg5159","article-title":"Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice","volume":"373","author":"Chen","year":"2021","journal-title":"Science"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1161\/CIRCRESAHA.110.227512","article-title":"Differentiation of human embryonic stem cells and induced pluripotent stem cells to cardiomyocytes: A methods overview","volume":"111","author":"Mummery","year":"2012","journal-title":"Circ Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3871","DOI":"10.1113\/JP282562","article-title":"Advances in induced pluripotent stem cell-derived cardiac myocytes: Technological breakthroughs, key discoveries and new applications","volume":"602","author":"Clancy","year":"2024","journal-title":"J. Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1038\/nprot.2017.033","article-title":"Differentiation of cardiomyocytes and generation of human engineered heart tissue","volume":"12","author":"Breckwoldt","year":"2017","journal-title":"Nat. Protoc."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Mahapatra, S., Sharma, M.V., Brownson, B., Gallicano, V.E., and Gallicano, G.I. (2022). Cardiac inducing colonies halt fibroblast activation and induce cardiac\/endothelial cells to move and expand via paracrine signaling. Mol. Biol. Cell, 33.","DOI":"10.1091\/mbc.E22-02-0032"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1755-1536-5-15","article-title":"Cardiac fibroblasts, fibrosis and extracellular matrix remodeling in heart disease","volume":"5","author":"Fan","year":"2012","journal-title":"Fibrogenes. Tissue Repair"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/s41536-021-00140-4","article-title":"Next generation of heart regenerative therapies: Progress and promise of cardiac tissue engineering","volume":"6","author":"Tenreiro","year":"2021","journal-title":"NPJ Regen. Med."},{"key":"ref_56","first-page":"920","article-title":"Collagen biomaterial for the treatment of myocardial infarction: An update on cardiac tissue engineering and myocardial regeneration","volume":"9","author":"Wu","year":"2019","journal-title":"Drug Deliv. Transl. Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1002\/(SICI)1097-0290(20000405)68:1<106::AID-BIT13>3.0.CO;2-3","article-title":"Three-dimensional engineered heart tissue from neonatal rat cardiac myocytes","volume":"68","author":"Zimmermann","year":"2000","journal-title":"Biotechnol. Bioeng."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/nm1394","article-title":"Engineered heart tissue grafts improve systolic and diastolic function in infarcted rat hearts","volume":"12","author":"Zimmermann","year":"2006","journal-title":"Nat. Med."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Farshidfar, N., Iravani, S., and Varma, R.S. (2023). Alginate-based biomaterials in tissue engineering and regenerative medicine. Mar. Drugs, 21.","DOI":"10.3390\/md21030189"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1002\/bit.10372","article-title":"Optimization of cardiac cell seeding and distribution in 3D porous alginate scaffolds","volume":"80","author":"Dar","year":"2002","journal-title":"Biotechnol. Bioeng."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"II-63","DOI":"10.1161\/circ.100.suppl_2.Ii-63","article-title":"Survival and Function of Bioengineered Cardiac Grafts","volume":"100","author":"Li","year":"1999","journal-title":"Circulation"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2305188","DOI":"10.1002\/adfm.202305188","article-title":"Formulation and Evaluation of PVA\/Gelatin\/Carrageenan Inks for 3D Printing and Development of Tissue-Engineered Heart Valves","volume":"34","author":"Jafari","year":"2024","journal-title":"Adv. Funct. Mater."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.biomaterials.2018.03.042","article-title":"Injectable hyaluronic acid based microrods provide local micromechanical and biochemical cues to attenuate cardiac fibrosis after myocardial infarction","volume":"169","author":"Le","year":"2018","journal-title":"Biomaterials"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Aziz, R., Falanga, M., Purenovic, J., Mancini, S., Lamberti, P., and Guida, M. (2023). A Review on the Applications of Natural Biodegradable Nano Polymers in Cardiac Tissue Engineering. Nanomaterials, 13.","DOI":"10.3390\/nano13081374"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Li, G., Zhao, M., Xu, F., Yang, B., Li, X., Meng, X., Teng, L., Sun, F., and Li, Y. (2020). Synthesis and biological application of polylactic acid. Molecules, 25.","DOI":"10.3390\/molecules25215023"},{"key":"ref_66","first-page":"60","article-title":"The development and challenges of poly (lactic acid) and poly (glycolic acid)","volume":"3","author":"Jem","year":"2020","journal-title":"Adv. Ind. Eng. Polym. Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.addr.2016.06.018","article-title":"Polylactic acid (PLA) controlled delivery carriers for biomedical applications","volume":"107","author":"Tyler","year":"2016","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S1053-2498(01)00349-7","article-title":"A novel small animal model of left ventricular tissue engineering","volume":"21","author":"Krupnick","year":"2002","journal-title":"J. Heart Lung Transplant."},{"key":"ref_69","first-page":"918","article-title":"Electrospun scaffold tailored for tissue-specific extracellular matrix","volume":"1","author":"Teo","year":"2006","journal-title":"Biotechnol. J. Healthc. Nutr. Technol."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Genovese, J.A., Spadaccio, C., Rainer, A., and Covino, E. (2011). Electrospun nanocomposites and stem cells in cardiac tissue engineering. Myocardial Tissue Engineering. Studies in Mechanobiology, Tissue Engineering and Biomaterials, Springer.","DOI":"10.1007\/8415_2011_69"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1802852","DOI":"10.1002\/adfm.201802852","article-title":"Electrospun fibrous architectures for drug delivery, tissue engineering and cancer therapy","volume":"29","author":"Ding","year":"2019","journal-title":"Adv. Funct. Mater."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"3343","DOI":"10.1002\/adma.200803092","article-title":"Progress in the field of electrospinning for tissue engineering applications","volume":"21","author":"Agarwal","year":"2009","journal-title":"Adv. Mater."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1002\/jbm.b.31862","article-title":"Guided orientation of cardiomyocytes on electrospun aligned nanofibers for cardiac tissue engineering","volume":"98","author":"Kai","year":"2011","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/nm1684","article-title":"Perfusion-decellularized matrix: Using nature\u2019s platform to engineer a bioartificial heart","volume":"14","author":"Ott","year":"2008","journal-title":"Nat. Med."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1161\/CIRCRESAHA.115.306874","article-title":"Bioengineering Human Myocardium on Native Extracellular Matrix","volume":"118","author":"Guyette","year":"2016","journal-title":"Circ. Res."},{"key":"ref_76","first-page":"70","article-title":"Current progress in 3D bioprinting of tissue analogs","volume":"24","author":"Zhang","year":"2019","journal-title":"SLAS Technol. Transl. Life Sci. Innov."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.2147\/IJN.S402855","article-title":"4D Thermo-Responsive Smart hiPSC-CM Cardiac Construct for Myocardial Cell Therapy","volume":"18","author":"Hann","year":"2023","journal-title":"Int. J. Nanomed."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/nnano.2010.246","article-title":"Nanotechnological strategies for engineering complex tissues","volume":"6","author":"Dvir","year":"2011","journal-title":"Nat. Nanotechnol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.12688\/f1000research.8237.1","article-title":"Advancing cardiovascular tissue engineering","volume":"5","author":"Truskey","year":"2016","journal-title":"F1000Research"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"13532","DOI":"10.1038\/s41598-018-31848-x","article-title":"A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes","volume":"8","author":"Maiullari","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1038\/nbt.3413","article-title":"A 3D bioprinting system to produce human-scale tissue constructs with structural integrity","volume":"34","author":"Kang","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Barbulescu, G.I., Bojin, F.M., Ordodi, V.L., Goje, I.D., Barbulescu, A.S., and Paunescu, V. (2022). Decellularized extracellular matrix scaffolds for cardiovascular tissue engineering: Current techniques and challenges. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232113040"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"2307","DOI":"10.1038\/ncomms3307","article-title":"Repopulation of decellularized mouse heart with human induced pluripotent stem cell-derived cardiovascular progenitor cells","volume":"4","author":"Lu","year":"2013","journal-title":"Nat. Commun."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"740","DOI":"10.3390\/polym3020740","article-title":"Hydrogels for cardiac tissue engineering","volume":"3","author":"Li","year":"2011","journal-title":"Polymers"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.actbio.2016.11.014","article-title":"Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering","volume":"48","author":"Kitsara","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1778","DOI":"10.1002\/ejhf.2631","article-title":"Accelerating cardiovascular research: Recent advances in translational 2D and 3D heart models","volume":"24","author":"Mohr","year":"2022","journal-title":"Eur. J. Heart Fail."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3390\/organoids1010008","article-title":"Metabolic studies in organoids: Current applications, Opportunities and Challenges","volume":"1","author":"Richiardone","year":"2022","journal-title":"Organoids"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.pbiomolbio.2017.07.003","article-title":"Electro-mechanical conditioning of human iPSC-derived cardiomyocytes for translational research","volume":"130","author":"Kroll","year":"2017","journal-title":"Prog. Biophys. Mol. Biol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1002\/adfm.201101905","article-title":"Self-assembling peptides as cell-interactive scaffolds","volume":"22","author":"Wu","year":"2012","journal-title":"Adv. Funct. Mater."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.msec.2010.12.017","article-title":"Biomimetic material strategies for cardiac tissue engineering","volume":"31","author":"Prabhakaran","year":"2011","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"363ra148","DOI":"10.1126\/scitranslmed.aaf8781","article-title":"Cardiac repair in guinea pigs with human engineered heart tissue from induced pluripotent stem cells","volume":"8","author":"Weinberger","year":"2016","journal-title":"Sci. Transl. Med."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1161\/CIRCRESAHA.116.310277","article-title":"Myocardial Tissue Engineering With Cells Derived From Human-Induced Pluripotent Stem Cells and a Native-Like, High-Resolution, 3-Dimensionally Printed Scaffold","volume":"120","author":"Gao","year":"2017","journal-title":"Circ. Res."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1161\/CIRCRESAHA.115.306985","article-title":"Human Engineered Heart Muscles Engraft and Survive Long Term in a Rodent Myocardial Infarction Model","volume":"117","author":"Riegler","year":"2015","journal-title":"Circ. Res."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"S29","DOI":"10.1161\/CIRCULATIONAHA.111.084343","article-title":"Feasibility, Safety, and Therapeutic Efficacy of Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Sheets in a Porcine Ischemic Cardiomyopathy Model","volume":"126","author":"Kawamura","year":"2012","journal-title":"Circulation"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/nature13233","article-title":"Human embryonic-stem-cell-derived cardiomyocytes regenerate non-human primate hearts","volume":"510","author":"Chong","year":"2014","journal-title":"Nature"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/nbt.4162","article-title":"Human embryonic stem cell\u2013derived cardiomyocytes restore function in infarcted hearts of non-human primates","volume":"36","author":"Liu","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Funakoshi, S., Miki, K., Takaki, T., Okubo, C., Hatani, T., Chonabayashi, K., Nishikawa, M., Takei, I., Oishi, A., and Narita, M. (2016). Enhanced engraftment, proliferation and therapeutic potential in heart using optimized human iPSC-derived cardiomyocytes. Sci. Rep., 6.","DOI":"10.1038\/srep19111"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"S145","DOI":"10.1161\/CIRCULATIONAHA.107.757286","article-title":"Endothelial Cell Coculture Within Tissue-Engineered Cardiomyocyte Sheets Enhances Neovascularization and Improves Cardiac Function of Ischemic Hearts","volume":"118","author":"Sekine","year":"2008","journal-title":"Circulation"},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Miyagawa, S., Kainuma, S., Kawamura, T., Suzuki, K., Ito, Y., Iseoka, H., Ito, E., Takeda, M., Sasai, M., and Mochizuki-Oda, N. (2022). Case report: Transplantation of human induced pluripotent stem cell-derived cardiomyocyte patches for ischemic cardiomyopathy. Front. Cardiovasc. Med., 9.","DOI":"10.3389\/fcvm.2022.950829"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.jacc.2017.11.047","article-title":"Transplantation of Human Embryonic Stem Cell-Derived Cardiovascular Progenitors for Severe Ischemic Left Ventricular Dysfunction","volume":"71","author":"Vanneaux","year":"2018","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_101","unstructured":"(2024, December 16). Safety and Efficacy of Induced Pluripotent Stem Cell-derived Engineered Human Myocardium as Biological Ventricular Assist Tissue in Terminal Heart Failure. ClinicalTrials.gov ID: NCT04396899, Available online: https:\/\/clinicaltrials.gov\/study\/NCT04396899."},{"key":"ref_102","unstructured":"(2024, December 16). Human Embryonic Stem Cell-Derived Cardiomyocyte Therapy for Chronic Ischemic Left Ventricular Dysfunction. ClinicalTrials.gov ID: NCT05068674, Available online: https:\/\/clinicaltrials.gov\/study\/NCT05068674."},{"key":"ref_103","first-page":"106","article-title":"Host response of implanted biomaterials","volume":"2015","author":"Rajesh","year":"2015","journal-title":"Biosurfaces A Mater. Sci. Eng. Perspect."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1080\/09723757.2004.11885875","article-title":"The human leukocyte antigen (HLA) system","volume":"4","author":"Shankarkumar","year":"2004","journal-title":"Int. J. Hum. Genet."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1093\/cvr\/cvz010","article-title":"ESC Working Group on Cellular Biology of the Heart: Position paper for Cardiovascular Research: Tissue engineering strategies combined with cell therapies for cardiac repair in ischaemic heart disease and heart failure","volume":"115","author":"Madonna","year":"2019","journal-title":"Cardiovasc. Res."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Rayat Pisheh, H., Nojabaei, F.S., Darvishi, A., Rayat Pisheh, A., and Sani, M. (2024). Cardiac tissue engineering: An emerging approach to the treatment of heart failure. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1441933"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1161\/CIRCRESAHA.117.310738","article-title":"Engineering cardiac muscle tissue: A maturating field of research","volume":"120","author":"Weinberger","year":"2017","journal-title":"Circ. Res."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.biomaterials.2015.01.067","article-title":"Functional maturation of human pluripotent stem cell derived cardiomyocytes in vitro\u2013correlation between contraction force and electrophysiology","volume":"51","author":"Ribeiro","year":"2015","journal-title":"Biomaterials"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1016\/j.jacc.2014.05.006","article-title":"Assessment of the 12-Lead electrocardiogram as a screening test for detection of cardiovascular disease in healthy general populations of young people (12\u201325 years of age) a scientific statement from the American Heart Association and the American College of Cardiology","volume":"64","author":"Maron","year":"2014","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_110","unstructured":"Ashraf, M. (2022). Development and Validation of Optical Mapping Instrumentation and Data Analysis Software for Use with Stem Cell-Derived Cardiomyocytes. [Ph.D. Thesis, University of British Columbia]."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"20417314221127908","DOI":"10.1177\/20417314221127908","article-title":"Molecular and electrophysiological evaluation of human cardiomyocyte subtypes to facilitate generation of composite cardiac models","volume":"13","author":"Li","year":"2022","journal-title":"J. Tissue Eng."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1038\/s44161-024-00472-6","article-title":"Challenges and perspectives of heart repair with pluripotent stem cell-derived cardiomyocytes","volume":"3","author":"Eschenhagen","year":"2024","journal-title":"Nat. Cardiovasc. Res."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1161\/CIRCRESAHA.118.311213","article-title":"Can we engineer a human cardiac patch for therapy?","volume":"123","author":"Zhang","year":"2018","journal-title":"Circ. Res."},{"key":"ref_114","doi-asserted-by":"crossref","unstructured":"Fassina, D., Costa, C.M., Longobardi, S., Karabelas, E., Plank, G., Harding, S.E., and Niederer, S.A. (2022). Modelling the interaction between stem cells derived cardiomyocytes patches and host myocardium to aid non-arrhythmic engineered heart tissue design. PLoS Comput. Biol., 18.","DOI":"10.1371\/journal.pcbi.1010030"},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1093\/cvr\/cvab075","article-title":"Alternative strategies in cardiac preclinical research and new clinical trial formats","volume":"118","author":"Kreutzer","year":"2022","journal-title":"Cardiovasc. Res."},{"key":"ref_116","doi-asserted-by":"crossref","unstructured":"Hawthorne, W.J. (2024). Ethical and legislative advances in xenotransplantation for clinical translation: Focusing on cardiac, kidney and islet cell xenotransplantation. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1355609"},{"key":"ref_117","unstructured":"Johnson, J.A. (2012). FDA Regulation of Medical Devices."},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"Feigal, E.G., and Cosenza, M.E. (2021). Cellular-based therapies. Translational Medicine, CRC Press.","DOI":"10.1201\/9781003124542-28"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1161\/CIRCRESAHA.121.319579","article-title":"Cardiac myoediting attenuates cardiac abnormalities in human and mouse models of Duchenne muscular dystrophy","volume":"129","author":"Atmanli","year":"2021","journal-title":"Circ. Res."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"584758","DOI":"10.1155\/2015\/584758","article-title":"Hypoxia inducible factor pathway and physiological adaptation: A cell survival pathway?","volume":"2015","author":"Kumar","year":"2015","journal-title":"Mediat. Inflamm."},{"key":"ref_121","unstructured":"Prondzynski, K.M. (2017). Modeling of Hypertrophic Cardiomyopathy and Assessment of Gene Therapy in Human iPSC-Derived Cardiomyocytes. [Ph.D. Thesis, Staats-und Universit\u00e4tsbibliothek Hamburg Carl von Ossietzky]."},{"key":"ref_122","unstructured":"Parmar, N. (2022). Investigating Targeted Therapies for Genetic Heart Disease. [Ph.D. Thesis, University of Toronto (Canada)]."},{"key":"ref_123","doi-asserted-by":"crossref","unstructured":"Dutton, L.C., Dudhia, J., Guest, D.J., and Connolly, D.J. (2024). CRISPR\/Cas9 gene editing in induced pluripotent stem cells to investigate the feline hypertrophic cardiomyopathy causing MYBPC3\/R820W mutation. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0311761"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"1","DOI":"10.33140\/JEMOA.02.01.06","article-title":"Exploring Biomedical Engineering (BME): Advances within Accelerated Computing and Regenerative Medicine for a Computational and Medical Science Perspective Exploration Analysis","volume":"2","author":"Akhtar","year":"2024","journal-title":"J. Emerg. Med. OA"},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1186\/s40001-023-01065-y","article-title":"Artificial intelligence in cardiovascular diseases: Diagnostic and therapeutic perspectives","volume":"28","author":"Sun","year":"2023","journal-title":"Eur. J. Med. Res."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"2235564","DOI":"10.1080\/07853890.2023.2235564","article-title":"Deep learning-derived 12-lead electrocardiogram-based genotype prediction for hypertrophic cardiomyopathy: A pilot study","volume":"55","author":"Chen","year":"2023","journal-title":"Ann. Med."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"e009056","DOI":"10.1161\/CIRCEP.120.009056","article-title":"Discovering and visualizing disease-specific electrocardiogram features using deep learning: Proof-of-concept in phospholamban gene mutation carriers","volume":"14","author":"Taha","year":"2021","journal-title":"Circ. Arrhythmia Electrophysiol."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"055011","DOI":"10.1088\/1361-6579\/ad46e2","article-title":"ELRL-MD: A deep learning approach for myocarditis diagnosis using cardiac magnetic resonance images with ensemble and reinforcement learning integration","volume":"45","author":"Kasmaee","year":"2024","journal-title":"Physiol. Meas."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"e007952","DOI":"10.1161\/CIRCEP.119.007952","article-title":"Artificial Intelligence and Machine Learning in Arrhythmias and Cardiac Electrophysiology","volume":"13","author":"Feeny","year":"2020","journal-title":"Circ. Arrhythmia Electrophysiol."},{"key":"ref_130","doi-asserted-by":"crossref","unstructured":"Nguyen, A.H., Marsh, P., Schmiess-Heine, L., Burke, P.J., Lee, A., Lee, J., and Cao, H. (2019). Cardiac tissue engineering: State-of-the-art methods and outlook. J. Biol. Eng., 13.","DOI":"10.1186\/s13036-019-0185-0"},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1093\/stcltm\/szae067","article-title":"Decoded cardiopoietic cell secretome linkage to heart repair biosignature","volume":"13","author":"Garmany","year":"2024","journal-title":"Stem. Cells Transl. Med."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1089\/biores.2015.0019","article-title":"Human mesenchymal stem cells reendothelialize porcine heart valve scaffolds: Novel perspectives in heart valve tissue engineering","volume":"4","author":"Lanuti","year":"2015","journal-title":"BioRes. Open Access"},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"Iii-44","DOI":"10.1161\/circ.102.suppl_3.III-44","article-title":"Functional Living Trileaflet Heart Valves Grown In Vitro","volume":"102","author":"Hoerstrup","year":"2000","journal-title":"Circulation"},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.stemcr.2016.04.011","article-title":"Human Engineered Heart Tissue: Analysis of Contractile Force","volume":"7","author":"Mannhardt","year":"2016","journal-title":"Stem Cell Rep."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"e2501","DOI":"10.1002\/pdi3.2501","article-title":"Cardiomyocyte proliferation and regeneration in congenital heart disease","volume":"2","author":"Liang","year":"2024","journal-title":"Pediatr. Discov."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"32","DOI":"10.5483\/BMBRep.2022-0185","article-title":"Engineered human cardiac tissues for modeling heart diseases","volume":"56","author":"Min","year":"2023","journal-title":"BMB Rep."},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/s41586-018-0016-3","article-title":"Advanced maturation of human cardiac tissue grown from pluripotent stem cells","volume":"556","author":"Ma","year":"2018","journal-title":"Nature"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/13\/24\/2098\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:54:41Z","timestamp":1760115281000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/13\/24\/2098"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,18]]},"references-count":137,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["cells13242098"],"URL":"https:\/\/doi.org\/10.3390\/cells13242098","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,18]]}}}