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This study utilized NSG-SGM3 mice to examine interactions between a human-derived mesothelioma reporter cell line (MZT-Luc2-mCherry) and the host\u2019s myeloid compartment. Tumor growth was assessed using optical tomography, while cytokine\/chemokine production was analyzed via multiplex assay. Histological and immunohistochemical analyses validated the epithelioid mesothelioma phenotype. In vitro mesothelioma cells secreted factors associated with myeloid cell chemoattraction and functions supporting the previously reported myeloid-biased secretory phenotype. In line with this, post-engraftment analysis revealed increased neutrophil-like Ly6G+ populations and decreased Ly6C+ inflammatory monocytes in the blood of tumor-bearing mice. Significant Ly6G+ cell infiltration was observed in the tumor, while CD11b+ myeloid cells were localized primarily in the tumor periphery. Tumor lysates showed increased levels of neutrophil chemoattractants and G-CSF, suggesting a previously not reported role of neutrophils in mesothelioma progression. This novel model provides a platform for studying mesothelioma\u2013host interactions, focusing on the myeloid compartment. It may also serve as a tool to facilitate the development of new therapeutic strategies targeting myeloid cell-mediated mechanisms in mesothelioma.<\/jats:p>","DOI":"10.3390\/cells13242135","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T09:13:38Z","timestamp":1734945218000},"page":"2135","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Myeloid Cell Mobilization and Recruitment by Human Mesothelioma in NSG-SGM3 Mice"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3165-6872","authenticated-orcid":false,"given":"Vadim V.","family":"Shindyapin","sequence":"first","affiliation":[{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"},{"name":"Department of Immunobiology and Biomedicine, Scientific Center of Genetics and Life Sciences, Sirius University of Science and Technology, Federal Territory Sirius, 354340 Krasnodar Krai, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina O.","family":"Gubernatorova","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"},{"name":"Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina A.","family":"Gorshkova","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"},{"name":"Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelya R.","family":"Chicherina","sequence":"additional","affiliation":[{"name":"Department of Immunobiology and Biomedicine, Scientific Center of Genetics and Life Sciences, Sirius University of Science and Technology, Federal Territory Sirius, 354340 Krasnodar Krai, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fedor A.","family":"Sysonov","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"},{"name":"Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia S.","family":"Yakovleva","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"},{"name":"Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"},{"name":"Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2851-3775","authenticated-orcid":false,"given":"Daria A.","family":"Bogdanova","sequence":"additional","affiliation":[{"name":"Department of Immunobiology and Biomedicine, Scientific Center of Genetics and Life Sciences, Sirius University of Science and Technology, Federal Territory Sirius, 354340 Krasnodar Krai, Russia"},{"name":"Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4323-7174","authenticated-orcid":false,"given":"Oleg N.","family":"Demidov","sequence":"additional","affiliation":[{"name":"Department of Immunobiology and Biomedicine, Scientific Center of Genetics and Life Sciences, Sirius University of Science and Technology, Federal Territory Sirius, 354340 Krasnodar Krai, Russia"},{"name":"Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg, Russia"},{"name":"INSERM UMR1231, University of Burgundy, 21078 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8170-1260","authenticated-orcid":false,"given":"Mariya V.","family":"Samsonova","sequence":"additional","affiliation":[{"name":"Pulmonology Research Institute, Federal Medical-Biological Agency of Russian Federation, 115682 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1039-4245","authenticated-orcid":false,"given":"Vladimir P.","family":"Baklaushev","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"},{"name":"Pulmonology Research Institute, Federal Medical-Biological Agency of Russian Federation, 115682 Moscow, Russia"},{"name":"Federal Research and Clinical Center for Specialized Types of Medical Care and Medical Technologies, Federal Medical-Biological Agency of Russian Federation, 123098 Moscow, Russia"},{"name":"Federal Center of Brain Research and Neurotechnologies, Federal Medical-Biological Agency of Russian Federation, 117513 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaukhar M.","family":"Yusubalieva","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"},{"name":"Federal Research and Clinical Center for Specialized Types of Medical Care and Medical Technologies, Federal Medical-Biological Agency of Russian Federation, 123098 Moscow, Russia"},{"name":"Federal Center of Brain Research and Neurotechnologies, Federal Medical-Biological Agency of Russian Federation, 117513 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina 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