{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:27:57Z","timestamp":1787239677848,"version":"build-2736575974"},"reference-count":32,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T00:00:00Z","timestamp":1736812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Foundation","doi-asserted-by":"publisher","award":["APP2004957"],"award-info":[{"award-number":["APP2004957"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Foundation","doi-asserted-by":"publisher","award":["2019Erber"],"award-info":[{"award-number":["2019Erber"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Raine Medical Research Foundation","award":["APP2004957"],"award-info":[{"award-number":["APP2004957"]}]},{"name":"Raine Medical Research Foundation","award":["2019Erber"],"award-info":[{"award-number":["2019Erber"]}]},{"DOI":"10.13039\/100012155","name":"Channel 7 Telethon Trust","doi-asserted-by":"publisher","award":["APP2004957"],"award-info":[{"award-number":["APP2004957"]}],"id":[{"id":"10.13039\/100012155","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012155","name":"Channel 7 Telethon Trust","doi-asserted-by":"publisher","award":["2019Erber"],"award-info":[{"award-number":["2019Erber"]}],"id":[{"id":"10.13039\/100012155","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Acute lymphoblastic leukaemia is the most common childhood malignancy that remains a leading cause of death in childhood. It may be characterised by multiple known recurrent genetic aberrations that inform prognosis, the most common being hyperdiploidy and t(12;21) ETV6::RUNX1. We aimed to assess the applicability of a new imaging flow cytometry methodology that incorporates cell morphology, immunophenotype, and fluorescence in situ hybridisation (FISH) to identify aneuploidy of chromosomes 4 and 21 and the translocation ETV6::RUNX1. We evaluated this new \u201cimmuno-flowFISH\u201d platform on 39 cases of paediatric ALL of B-lineage known to have aneuploidy of chromosomes 4 and 21 and the translocation ETV6::RUNX1. After identifying the leukaemic population based on immunophenotype (i.e., expression of CD34, CD10, and CD19 antigens), we assessed for copy numbers of loci for the centromeres of chromosomes 4 and 21 and the ETV6 and RUNX1 regions using fluorophore-labelled DNA probes in more than 1000 cells per sample. Trisomy 4 and 21, tetrasomy 21, and translocations of ETV6::RUNX1, as well as gains and losses of ETV6 and RUNX1, could all be identified based on FISH spot counts and digital imagery. There was variability in clonal makeup in individual cases, suggesting the presence of sub-clones. Copy number alterations and translocations could be detected even when the cell population comprised less than 1% of cells and included cells with a mature B-cell phenotype, i.e., CD19-positive, lacking CD34 and CD10. In this proof-of-principle study of 39 cases, this sensitive and specific semi-automated high-throughput imaging flow cytometric immuno-flowFISH method has been able to show that alterations in ploidy and ETV6::RUNX1 could be detected in the 39 cases of paediatric ALL. This imaging flow cytometric FISH method has potential applications for diagnosis and monitoring disease and marrow regeneration (i.e., distinguishing residual ALL from regenerating haematogones) following chemotherapy.<\/jats:p>","DOI":"10.3390\/cells14020114","type":"journal-article","created":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T06:13:07Z","timestamp":1736835187000},"page":"114","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Imaging Flow Cytometric Identification of Chromosomal Defects in Paediatric Acute Lymphoblastic Leukaemia"],"prefix":"10.3390","volume":"14","author":[{"given":"Ana P. A.","family":"Simpson","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences, The University of Western Australia, Crawley, WA 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4982-4871","authenticated-orcid":false,"given":"Carly E.","family":"George","sequence":"additional","affiliation":[{"name":"Department of Clinical Haematology, Oncology, Blood and Marrow Transplantation, Perth Children\u2019s Hospital, Perth, WA 6009, Australia"},{"name":"PathWest Laboratory Medicine, Nedlands, WA 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry Y. L.","family":"Hui","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, The University of Western Australia, Crawley, WA 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi","family":"Doddi","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, The University of Western Australia, Crawley, WA 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1836-4075","authenticated-orcid":false,"given":"Rishi S.","family":"Kotecha","sequence":"additional","affiliation":[{"name":"Department of Clinical Haematology, Oncology, Blood and Marrow Transplantation, Perth Children\u2019s Hospital, Perth, WA 6009, Australia"},{"name":"Medical School, The University of Western Australia, Crawley, WA 6009, Australia"},{"name":"Curtin Medical School, Curtin University, Bentley, WA 6102, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy A.","family":"Fuller","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, The University of Western Australia, Crawley, WA 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1028-9376","authenticated-orcid":false,"given":"Wendy N.","family":"Erber","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, The University of Western Australia, Crawley, WA 6009, Australia"},{"name":"PathWest Laboratory Medicine, Nedlands, WA 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mohammadian-Hafshejani, A., Farber, I.M., and Kheiri, S. 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