{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T21:13:10Z","timestamp":1782681190833,"version":"3.54.5"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T00:00:00Z","timestamp":1739836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["R01AI051645"],"award-info":[{"award-number":["R01AI051645"]}]},{"name":"NIH","award":["R37AI020241"],"award-info":[{"award-number":["R37AI020241"]}]},{"name":"MWV Leukocyte Research Fund (PFW)","award":["R01AI051645"],"award-info":[{"award-number":["R01AI051645"]}]},{"name":"MWV Leukocyte Research Fund (PFW)","award":["R37AI020241"],"award-info":[{"award-number":["R37AI020241"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>High-affinity IgE receptors (Fc\u03b5RI) are expressed on human blood eosinophils and may be upregulated on eosinophils at sites of allergic inflammation including atopic dermatitis and allergic asthma. Fc\u03b5RI engagement, however, fails to elicit \u201ceffector\u201d responses from eosinophils. Thus, a functional role for Fc\u03b5RI on eosinophils has been uncertain. We evaluated the role of Fc\u03b5RI in enhancing eosinophil antigen presentation in vivo by using humanized Fc\u03b5RI \u03b1 chain (hFc\u03b5RI\u03b1) transgenic mice. Eosinophils from hFc\u03b5RI\u03b1 transgenic mice expressed humanized Fc\u03b5RI\u03b1, with higher levels of eosinophils from the bronchoalveolar lavage of experimental asthma than those from polymyxin-elicited peritoneal lavage. The hFc\u03b5RI\u03b1-bearing eosinophils instilled intratracheally (i.t.) into recipient wild-type mice migrated from airways into paratracheal lymph nodes (pLNs) and spleens. Eosinophils, pretreated in vitro with nitrophenyl-ovalbumin ((NP)-OVA) and anti-NP human IgE complexes and instilled i.t., presented NP antigen via hFc\u03b5RI\u03b1 to T cells more effectively than those pretreated with NP-OVA only, as assessed by pLN cell proliferation. IgE\/Fc\u03b5RI\u03b1-facilitated eosinophil antigen presentation resulted in increased IL-4 but not INF-\u03b3 production by pLN cells, with a bias towards Th2 cytokine production. Furthermore, cross-linking hFc\u03b5RI\u03b1 on eosinophils increased eosinophil expressions of T cell costimulatory proteins CD40, CD80, and CD86. Humanized Fc\u03b5RI\u03b1 on murine eosinophils functions to enhance eosinophil antigen presentation capacities by mediating IgE-facilitated antigen presentation and upregulating expression of requisite T cell costimulatory proteins. Thus, a functional, non-\u201ceffector\u201d role for Fc\u03b5RI on eosinophils is revealed through identifying a means by which IgE may act on eosinophils to mediate their immunomodulatory, enhanced antigen presentation capabilities.<\/jats:p>","DOI":"10.3390\/cells14040301","type":"journal-article","created":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T03:47:06Z","timestamp":1739850426000},"page":"301","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Humanized Fc\u03b5RI Expressed on Mouse Eosinophils Mediates IgE-Facilitated Eosinophil Antigen Presentation"],"prefix":"10.3390","volume":"14","author":[{"given":"Haibin","family":"Wang","sequence":"first","affiliation":[{"name":"Division of Allergy and Inflammation, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, CLS 943, Boston, MA 02215, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1475-3364","authenticated-orcid":false,"given":"Jean-Pierre","family":"Kinet","sequence":"additional","affiliation":[{"name":"Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, CLS 943, Boston, MA 02215, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9580-560X","authenticated-orcid":false,"given":"Peter F.","family":"Weller","sequence":"additional","affiliation":[{"name":"Division of Allergy and Inflammation, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, CLS 943, Boston, MA 02215, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1146\/annurev-immunol-061020-053712","article-title":"IgE, IgE Receptors and Anti-IgE Biologics: Protein Structures and Mechanisms of Action","volume":"41","author":"McDonnell","year":"2023","journal-title":"Annu. 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