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Meyer Children\u2019s University Hospital, Florence, Italy","award":["104 del 02\/02\/2022"],"award-info":[{"award-number":["104 del 02\/02\/2022"]}]},{"name":"Meyer Foundation, A. Meyer Children\u2019s University Hospital, Florence, Italy","award":["n. 0106182"],"award-info":[{"award-number":["n. 0106182"]}]},{"name":"Meyer Foundation, A. Meyer Children\u2019s University Hospital, Florence, Italy","award":["n. B\/2022\/0198"],"award-info":[{"award-number":["n. B\/2022\/0198"]}]},{"name":"Intesa San Paolo, Torino, Italy","award":["PRIN 2022 D.D"],"award-info":[{"award-number":["PRIN 2022 D.D"]}]},{"name":"Intesa San Paolo, Torino, Italy","award":["104 del 02\/02\/2022"],"award-info":[{"award-number":["104 del 02\/02\/2022"]}]},{"name":"Intesa San Paolo, Torino, Italy","award":["n. 0106182"],"award-info":[{"award-number":["n. 0106182"]}]},{"name":"Intesa San Paolo, Torino, Italy","award":["n. B\/2022\/0198"],"award-info":[{"award-number":["n. B\/2022\/0198"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The beta-3 adrenergic receptor (\u03b23-AR), whose expression is modulated by oxygen levels, was found to play a key role in organ maturation, and its agonism was reported to mitigate hyperoxia-induced large bowel damage by preventing organ hypoplasia, preserving epithelial integrity, vascularization, and the neurochemical coding in the colonic myenteric plexus. This study explored the effects of \u03b23-AR agonism in preventing hyperoxia-related alterations on the ileal enteric nervous system (ENS). Sprague\u2013Dawley rat pups were reared under normoxia or hyperoxia (85%) during the first two weeks after birth and treated or not with the \u03b23-AR agonist BRL37344 at 1, 3, or 6 mg\/kg. Hyperoxia caused an imbalance of inhibitory nitrergic and excitatory cholinergic neurons in both the myenteric and submucosal plexuses and decreased the amounts of neurons in the submucosal plexus and that of S100\u03b2+ and GFAP+ glial cells in the myenteric plexus. Administration of 3 mg\/kg BRL37344 preserved the neuronal chemical coding and partially prevented the loss of myenteric GFAP+ glial cells, while it did not counteract submucosal neuronal loss. Our findings indicate the potential of \u03b23-AR agonism as a new therapeutic strategy for hyperoxia-induced ileal ENS alterations.<\/jats:p>","DOI":"10.3390\/cells14070475","type":"journal-article","created":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T13:48:20Z","timestamp":1742824100000},"page":"475","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Beta-3 Adrenoceptor Agonism Protects the Enteric Nervous Tissue Against Hyperoxia-Induced Damage"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2374-5147","authenticated-orcid":false,"given":"Patrizia","family":"Nardini","sequence":"first","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5310-9147","authenticated-orcid":false,"given":"Luca","family":"Filippi","sequence":"additional","affiliation":[{"name":"Neonatology and Neonatal Intensive Care Unit, Department of Clinical and Experimental Medicine, University of Pisa, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1074-1681","authenticated-orcid":false,"given":"Virginia","family":"Zizi","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Molino","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3419-4600","authenticated-orcid":false,"given":"Camilla","family":"Fazi","sequence":"additional","affiliation":[{"name":"Department of Pediatric, Meyer Children\u2019s University Hospital, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Chivetti","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9828-2082","authenticated-orcid":false,"given":"Alessandro","family":"Pini","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1126\/science.2570461","article-title":"Molecular Characterization of the Human Beta 3-Adrenergic Receptor","volume":"245","author":"Emorine","year":"1989","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1002\/j.1550-8528.1994.tb00089.x","article-title":"Comparison of the Lipolytic Effects of Norepinephrine and BRL 37344 in Rat Brown and White Adipocytes","volume":"2","author":"Simard","year":"1994","journal-title":"Obes. 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