{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:36:58Z","timestamp":1787027818357,"version":"build-2736575974"},"reference-count":124,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T00:00:00Z","timestamp":1747267200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"German Research Foundation","award":["ME 1706\/18"],"award-info":[{"award-number":["ME 1706\/18"]}]},{"name":"Sonnenfeld-Stiftung (Berlin, Germany)","award":["ME 1706\/18"],"award-info":[{"award-number":["ME 1706\/18"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>NGF plays important roles in ocular surface homeostasis and different pathological conditions. One effect includes promoting conjunctival epithelial cell differentiation and mucin secretion. This study characterizes the individual roles of TRPV1 and TRPM8 channel activity in mediating the effects of NGF on intracellular Ca2+ regulation and its alteration of regulatory cell volume responses to anisosmotic challenges in human conjunctival epithelial cells (IOBA-NHC). With fura-2\/AM-loaded cells, the effects of 40 \u00b5M capsaicin and 20 \u00b5M AMG 9810 on Ca2+ regulation confirm functional TRPV1 expression. TRPM8 expression is evident since 500 \u00b5M menthol and 20 \u00b5M AMTB have opposing effects on [Ca2+]i. AMG 9810 and AMTB (both 20 \u00b5M) suppress the responses to NGF (100 ng\/mL). With calcein\/AM-loaded cells, the effects of these mediators are evaluated on apparent cell volume responses induced by an anisosmotic challenge. NGF decreases the apparent cell volume that AMG 9810 suppresses, whereas AMTB (both 20 \u00b5M) augments this response. Therefore, NGF interacts with TRPV1 and TRPM8 to induce opposing effects on cell volume regulatory behavior. These opposing effects suggest that the signaling pathways and effectors that mediate responses to TRPV1 and TRPM8 activation are not the same.<\/jats:p>","DOI":"10.3390\/cells14100719","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T06:10:44Z","timestamp":1747289444000},"page":"719","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Nerve Growth Factor Modulates Regulatory Cell Volume Behavior via Stimulating TRPV1, TRPM8 Channels and Inducing Ca2+ Signaling in Human Conjunctival Epithelial Cells"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6322-8034","authenticated-orcid":false,"given":"Friedrich","family":"Wolf","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Campus Virchow-Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9041-2249","authenticated-orcid":false,"given":"Tina","family":"Dietrich-Ntoukas","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Campus Virchow-Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3494-4523","authenticated-orcid":false,"given":"Peter S.","family":"Reinach","sequence":"additional","affiliation":[{"name":"School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou 325015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5569-1473","authenticated-orcid":false,"given":"Uwe","family":"Pleyer","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Campus Virchow-Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8608-648X","authenticated-orcid":false,"given":"Stefan","family":"Mergler","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Campus Virchow-Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1097\/ICO.0000000000002910","article-title":"Observation of Conjunctiva-Associated Lymphoid Tissue with In Vivo Confocal Microscopy in Healthy Patients and Patients with Meibomian Gland Dysfunction","volume":"41","author":"Liu","year":"2022","journal-title":"Cornea"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1159\/000099252","article-title":"Anatomy and immunology of the ocular surface","volume":"92","author":"Knop","year":"2007","journal-title":"Chem. 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