{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:33:54Z","timestamp":1790019234081,"version":"4.0.1"},"reference-count":72,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T00:00:00Z","timestamp":1748476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Arginine methylation is a crucial post-translational modification (PTM) that plays a significant role in various biological processes. It occurs in two primary forms: mono-methylation (MMA) and di-methylation (DMA), with the latter further classified into symmetric (SDMA) and asymmetric methylation (ADMA). This review examines the functional implications of these methylation states, current detection methodologies, proteomics-based analytical approaches, and the different impacts of these methylations on protein function. Finally, the role of protein arginine methyltransferases (PRMTs) and their substrate specificity in shaping the arginine methylome are discussed.<\/jats:p>","DOI":"10.3390\/cells14110796","type":"journal-article","created":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T04:46:38Z","timestamp":1748493998000},"page":"796","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Functional Dynamics of Arginine Mono- and Di-Methylation"],"prefix":"10.3390","volume":"14","author":[{"given":"Xi\u2019ang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Division of Hematology, Oncology and Palliative Care, Massey Comprehensive Cancer Center, School of Medicine, Virginia Commonwealth University, Richmond, VI 23298, USA"},{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2829-2925","authenticated-orcid":false,"given":"Bin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Pulmonary Disease and Critical Care, Massey Comprehensive Cancer Center, School of Medicine, Virginia Commonwealth University, Richmond, VI 23298, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5948-9291","authenticated-orcid":false,"given":"Robert","family":"Winn","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Pulmonary Disease and Critical Care, Massey Comprehensive Cancer Center, School of Medicine, Virginia Commonwealth University, Richmond, VI 23298, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6284-4389","authenticated-orcid":false,"given":"Shanfa","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2414-0609","authenticated-orcid":false,"given":"Hengbin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Hematology, Oncology and Palliative Care, Massey Comprehensive Cancer Center, School of Medicine, Virginia Commonwealth University, Richmond, VI 23298, USA"},{"name":"Department of Internal Medicine, Division of Pulmonary Disease and Critical Care, Massey Comprehensive Cancer Center, School of Medicine, Virginia Commonwealth University, Richmond, VI 23298, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1038\/nsmb.1842","article-title":"Post-translational modifications in signal integration","volume":"17","author":"Deribe","year":"2010","journal-title":"Nat. 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