{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T11:13:28Z","timestamp":1789816408894,"version":"4.0.1"},"reference-count":77,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Center for Advancing Translational Sciences","award":["KL2 TR002015"],"award-info":[{"award-number":["KL2 TR002015"]}]},{"name":"Hyundai Hope on Wheels Scholar Grant","award":["KL2 TR002015"],"award-info":[{"award-number":["KL2 TR002015"]}]},{"name":"Four Diamonds Fund of the Pennsylvania State University College of Medicine","award":["KL2 TR002015"],"award-info":[{"award-number":["KL2 TR002015"]}]},{"name":"John Wawrynovic Leukemia Research Scholar Endowment","award":["KL2 TR002015"],"award-info":[{"award-number":["KL2 TR002015"]}]},{"name":"St. Baldrick\u2019s Foundation","award":["KL2 TR002015"],"award-info":[{"award-number":["KL2 TR002015"]}]},{"name":"Team Connor and Rally Foundation","award":["KL2 TR002015"],"award-info":[{"award-number":["KL2 TR002015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Casein Kinase II (CK2) is a ubiquitously present serine\/threonine kinase essential for mammalian development. CK2 holoenzyme is a tetramer with two highly related catalytic subunits (\u03b1 or \u03b1\u2019) and two regulatory \u00df subunits. Global deletion of the \u03b1 or \u03b2 subunit in mice is embryonically lethal. We and others have shown that CK2 is overexpressed in leukemia cells and plays an important role in cell cycle, survival, and resistance to the apoptosis of leukemia stem cells (LSCs). To study the role of CK2\u03b1 in adult mouse hematopoiesis, we generated hematopoietic cell-specific CK2\u03b1-conditional knockout mice (Vav-iCreCK2 f\/f). Here we report the generation and validation of a novel mouse model that lacks CK2\u03b1 in the hematopoietic compartment. Vav-iCreCK2\u03b1 f\/f mice were viable without dysmorphic features and showed a mild phenotype under baseline conditions. In Vav-iCreCK2\u03b1 f\/f mice, the blood count showed a significant decrease in total red blood cells and platelets. The spleen was enlarged in Vav-iCreCK2\u03b1 f\/f mice with evidence of extramedullary hematopoiesis. HSC and early progenitor cell compartments showed expansion in CK2\u03b1-null bone marrow, suggesting that the absence of CK2\u03b1 impaired their proliferation and differentiation. Given the established roles of CK2 in cell cycle regulation and the findings reported here, further functional studies are warranted to investigate the role of CK2\u03b1 in HSC self-renewal and differentiation. This mouse model serves as a valuable tool for understanding the role of CK2\u03b1 in normal and malignant hematopoiesis.<\/jats:p>","DOI":"10.3390\/cells14130963","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T10:44:41Z","timestamp":1750761881000},"page":"963","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["CK2\u03b1 Deletion in the Hematopoietic Compartment Shows a Mild Alteration in Terminally Differentiated Cells and the Expansion of Stem Cells"],"prefix":"10.3390","volume":"14","author":[{"given":"Rajesh","family":"Rajaiah","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Milton S. Hershey Medical Center,  Pennsylvania State University College of Medicine Hershey, Hershey, PA 17033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5663-5211","authenticated-orcid":false,"given":"Muhammad","family":"Daniyal","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Milton S. Hershey Medical Center,  Pennsylvania State University College of Medicine Hershey, Hershey, PA 17033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5217-2950","authenticated-orcid":false,"given":"Marudhu Pandiyan","family":"Shanmugam","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Milton S. Hershey Medical Center,  Pennsylvania State University College of Medicine Hershey, Hershey, PA 17033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Valensi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Milton S. Hershey Medical Center,  Pennsylvania State University College of Medicine Hershey, Hershey, PA 17033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koby","family":"Duke","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Milton S. Hershey Medical Center,  Pennsylvania State University College of Medicine Hershey, Hershey, PA 17033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Mercer","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Milton S. Hershey Medical Center,  Pennsylvania State University College of Medicine Hershey, Hershey, PA 17033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8373-7838","authenticated-orcid":false,"given":"Morgann","family":"Klink","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Milton S. Hershey Medical Center,  Pennsylvania State University College of Medicine Hershey, Hershey, PA 17033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Lanza","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine, Pennsylvania State University College of Medicine Hershey, Hershey, PA 17033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasin","family":"Uzun","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Milton S. Hershey Medical Center,  Pennsylvania State University College of Medicine Hershey, Hershey, PA 17033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suming","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Milton S. Hershey Medical Center,  Pennsylvania State University College of Medicine Hershey, Hershey, PA 17033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinisa","family":"Dovat","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Milton S. Hershey Medical Center,  Pennsylvania State University College of Medicine Hershey, Hershey, PA 17033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandrika Gowda","family":"Behura","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Milton S. Hershey Medical Center,  Pennsylvania State University College of Medicine Hershey, Hershey, PA 17033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/s41392-021-00567-7","article-title":"Protein kinase CK2: A potential therapeutic target for diverse human diseases","volume":"6","author":"Borgo","year":"2021","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.3892\/br.2016.829","article-title":"Protein kinase CK2 in development and differentiation","volume":"6","author":"Montenarh","year":"2017","journal-title":"Biomed. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Hong, H., and Benveniste, E.N. (2021). The immune regulatory role of protein kinase CK2 and its implications for treatment of cancer. Biomedicines, 9.","DOI":"10.3390\/biomedicines9121932"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1096\/fasebj.9.5.7896000","article-title":"Protein kinase CK2: An enzyme with multiple substrates and a puzzling regulation","volume":"9","author":"Allende","year":"1995","journal-title":"FASEB J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3873","DOI":"10.1242\/jcs.00074","article-title":"Protein kinase CK2: A challenge to canons","volume":"115","author":"Pinna","year":"2002","journal-title":"J. Cell Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"8436","DOI":"10.1021\/bi00488a034","article-title":"Isolation and characterization of human cDNA clones encoding the. alpha. and the. alpha.\u2019subunits of casein kinase II","volume":"29","author":"Lozeman","year":"1990","journal-title":"Biochemistry"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Bibby, A.C., and Litchfield, D.W. (2005). The multiple personalities of the regulatory subunit of protein kinase CK2: CK2 dependent and CK2 independent roles reveal a secret identity for CK2\u03b2. Int. J. Biol. Sci., 1.","DOI":"10.7150\/ijbs.1.67"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5693","DOI":"10.1073\/pnas.0802065105","article-title":"Protein kinase CK2 as an ectokinase: The role of the regulatory CK2\u03b2 subunit","volume":"105","author":"Rodriguez","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1128\/MCB.23.3.908-915.2003","article-title":"Disruption of the regulatory \u03b2 subunit of protein kinase CK2 in mice leads to a cell-autonomous defect and early embryonic lethality","volume":"23","author":"Buchou","year":"2003","journal-title":"Mol. Cell. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s11010-011-0961-8","article-title":"CK2\u03b1 is essential for embryonic morphogenesis","volume":"356","author":"Dominguez","year":"2011","journal-title":"Mol. Cell. Biochem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s00441-010-1068-3","article-title":"Cellular regulators of protein kinase CK2","volume":"342","author":"Montenarh","year":"2010","journal-title":"Cell Tissue Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Halloran, D., Pandit, V., and Nohe, A. (2022). The role of protein kinase CK2 in development and disease progression: A critical review. J. Dev. Biol., 10.","DOI":"10.3390\/jdb10030031"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"17029","DOI":"10.1074\/jbc.M114.624106","article-title":"Casein kinase 2 (CK2)-mediated phosphorylation of Hsp90\u03b2 as a novel mechanism of rifampin-induced MDR1 expression","volume":"290","author":"Kim","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.ajpath.2023.01.016","article-title":"Protein Kinase CK2 promotes proliferation, abnormal differentiation, and proinflammatory cytokine production of keratinocytes via regulation of STAT3 and Akt pathways in psoriasis","volume":"193","author":"Huang","year":"2023","journal-title":"Am. J. Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Klink, M., Rahman, M.A., Song, C., Dhanyamraju, P.K., Ehudin, M., Ding, Y., Steffens, S., Bhadauria, P., Iyer, S., and Aliaga, C. (2021). mechanistic basis for in vivo therapeutic efficacy of CK2 inhibitor CX-4945 in acute myeloid leukemia. Cancers, 13.","DOI":"10.3390\/cancers13051127"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Nu\u00f1ez de Villavicencio-Diaz, T., Rabalski, A.J., and Litchfield, D.W. (2017). Protein kinase CK2: Intricate relationships within regulatory cellular networks. Pharmaceuticals, 10.","DOI":"10.3390\/ph10010027"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Chua, M.M., Ortega, C.E., Sheikh, A., Lee, M., Abdul-Rassoul, H., Hartshorn, K.L., and Dominguez, I. (2017). CK2 in cancer: Cellular and biochemical mechanisms and potential therapeutic target. Pharmaceuticals, 10.","DOI":"10.3390\/ph10010018"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.it.2017.12.002","article-title":"Protein kinase CK2: An emerging regulator of immunity","volume":"39","author":"Gibson","year":"2018","journal-title":"Trends Immunol."},{"key":"ref_19","first-page":"685","article-title":"Protein kinase CK2 subunits are positive regulators of AKT kinase","volume":"28","author":"Guerra","year":"2006","journal-title":"Int. J. Oncol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.cyto.2018.01.021","article-title":"Regulation of hematopoiesis by the chemokine system","volume":"109","author":"Bonavita","year":"2018","journal-title":"Cytokine"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/S0301-472X(99)00024-7","article-title":"Development of the hematopoietic system in the mouse","volume":"27","author":"Keller","year":"1999","journal-title":"Exp. Hematol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2230","DOI":"10.1002\/pbc.25221","article-title":"Protein Phosphatase 1 (PP1) and Casein Kinase II (CK2) regulate Ikaros-mediated repression of TdT in thymocytes and T-cell leukemia","volume":"61","author":"Wang","year":"2014","journal-title":"Pediatr. Blood Cancer"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s11010-011-0964-5","article-title":"Ikaros, CK2 kinase, and the road to leukemia","volume":"356","author":"Dovat","year":"2011","journal-title":"Mol. Cell. Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"799","DOI":"10.4049\/jimmunol.2100059","article-title":"Protein kinase CK2 regulates B cell development and differentiation","volume":"207","author":"Wei","year":"2021","journal-title":"J. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"674","DOI":"10.3324\/haematol.2009.011999","article-title":"Regulation of PTEN by CK2 and Notch1 in primary T-cell acute lymphoblastic leukemia: Rationale for combined use of CK2-and \u03b3-secretase inhibitors","volume":"95","author":"Silva","year":"2009","journal-title":"Haematologica"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.cell.2006.05.041","article-title":"A CK2-dependent mechanism for degradation of the PML tumor suppressor","volume":"126","author":"Scaglioni","year":"2006","journal-title":"Cell"},{"key":"ref_27","first-page":"3065","article-title":"Ser\/Thr phosphorylation of hematopoietic specific protein 1 (HS1) Implication of protein kinase CK2","volume":"267","author":"Ruzzene","year":"2000","journal-title":"Eur. J. Biochem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"8291","DOI":"10.1074\/jbc.M707906200","article-title":"Recruitment of ikaros to pericentromeric heterochromatin is regulated by phosphorylation","volume":"283","author":"Gurel","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Song, C., Li, Z., Erbe, A.K., Savic, A., and Dovat, S. (2011). Regulation of Ikaros function by casein kinase 2 and protein phosphatase 1. World J. Biol. Chem., 2.","DOI":"10.4331\/wjbc.v2.i6.126"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1038\/leu.2015.331","article-title":"Epigenetic regulation of gene expression by Ikaros, HDAC1 and Casein Kinase II in leukemia","volume":"30","author":"Song","year":"2016","journal-title":"Leukemia"},{"key":"ref_31","first-page":"1520","article-title":"IKAROS and CK2 regulate expression of BCL-XL and chemosensitivity in high-risk B-cell acute lymphoblastic leukemia","volume":"136","author":"Song","year":"2020","journal-title":"Blood J. Am. Soc. Hematol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1038\/leu.2016.209","article-title":"Protein kinase CK2 regulates AKT, NF-\u03baB and STAT3 activation, stem cell viability and proliferation in acute myeloid leukemia","volume":"31","author":"Brancalion","year":"2017","journal-title":"Leukemia"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1038\/leu.2011.385","article-title":"Protein kinase CK2 in hematologic malignancies: Reliance on a pivotal cell survival regulator by oncogenic signaling pathways","volume":"26","author":"Piazza","year":"2012","journal-title":"Leukemia"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Manni, S., Brancalion, A., Mandato, E., Tubi, L.Q., Colpo, A., Pizzi, M., Cappellesso, R., Zaffino, F., Di Maggio, S.A., and Cabrelle, A. (2013). Protein kinase CK2 inhibition down modulates the NF-\u03baB and STAT3 survival pathways, enhances the cellular proteotoxic stress and synergistically boosts the cytotoxic effect of bortezomib on multiple myeloma and mantle cell lymphoma cells. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0075280"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1182\/blood-2005-11-013672","article-title":"Multiple myeloma cell survival relies on high activity of protein kinase CK2","volume":"108","author":"Piazza","year":"2006","journal-title":"Blood"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"6915","DOI":"10.1038\/sj.onc.1210495","article-title":"Pharmacological inhibition of protein kinase CK2 reverts the multidrug resistance phenotype of a CEM cell line characterized by high CK2 level","volume":"26","author":"Brustolon","year":"2007","journal-title":"Oncogene"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1038\/leu.2013.226","article-title":"Targeting the PI3K\/Akt\/mTOR signaling pathway in B-precursor acute lymphoblastic leukemia and its therapeutic potential","volume":"28","author":"Neri","year":"2014","journal-title":"Leukemia"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/leu.2017.301","article-title":"Therapeutic targeting of CK2 in acute and chronic leukemias","volume":"32","author":"Buontempo","year":"2018","journal-title":"Leukemia"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1016\/j.molonc.2013.08.006","article-title":"Aberrant signalling by protein kinase CK2 in imatinib-resistant chronic myeloid leukaemia cells: Biochemical evidence and therapeutic perspectives","volume":"7","author":"Borgo","year":"2013","journal-title":"Mol. Oncol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Quotti Tubi, L., Mandato, E., Canovas Nunes, S., Arjomand, A., Zaffino, F., Manni, S., Casellato, A., Macaccaro, P., Vitulo, N., and Zumerle, S. (2023). CK2\u03b2-regulated signaling controls B cell differentiation and function. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.959138"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Larson, S.R., Bortell, N., Illies, A., Crisler, W.J., Matsuda, J.L., and Lenz, L.L. (2020). Myeloid cell CK2 regulates inflammation and resistance to bacterial infection. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.590266"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"383","DOI":"10.4049\/jimmunol.1701592","article-title":"CK2 controls Th17 and regulatory T cell differentiation through inhibition of FoxO1","volume":"201","author":"Gibson","year":"2018","journal-title":"J. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1002\/gene.10161","article-title":"VavCre transgenic mice: A tool for mutagenesis in hematopoietic and endothelial lineages","volume":"34","author":"Georgiades","year":"2002","journal-title":"Genesis"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.ccell.2016.05.011","article-title":"MLL-AF9 expression in hematopoietic stem cells drives a highly invasive AML expressing EMT-related genes linked to poor outcome","volume":"30","author":"Stavropoulou","year":"2016","journal-title":"Cancer Cell"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.ccr.2006.08.020","article-title":"Identification and characterization of leukemia stem cells in murine MLL-AF9 acute myeloid leukemia","volume":"10","author":"Somervaille","year":"2006","journal-title":"Cancer Cell"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Almosailleakh, M., and Schwaller, J. (2019). Murine models of acute myeloid leukaemia. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20020453"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.biopsych.2012.11.013","article-title":"Selective knockout of the casein kinase 2 in d1 medium spiny neurons controls dopaminergic function","volume":"74","author":"Rebholz","year":"2013","journal-title":"Biol. Psychiatry"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.stem.2013.10.010","article-title":"Deciphering hematopoietic stem cells in their niches: A critical appraisal of genetic models, lineage tracing, and imaging strategies","volume":"13","author":"Joseph","year":"2013","journal-title":"Cell Stem Cell"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: Ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"D966","DOI":"10.1093\/nar\/gkae1078","article-title":"GENCODE 2025: Reference gene annotation for human and mouse","volume":"53","author":"Mudge","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1038\/nprot.2012.016","article-title":"Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks","volume":"7","author":"Trapnell","year":"2012","journal-title":"Nat. Protoc."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkz114","article-title":"The R package Rsubread is easier, faster, cheaper and better for alignment and quantification of RNA sequencing reads","volume":"47","author":"Liao","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: A Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1038\/s41467-019-09234-6","article-title":"Metascape provides a biologist-oriented resource for the analysis of systems-level datasets","volume":"10","author":"Zhou","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1128\/MCB.01119-07","article-title":"The alpha catalytic subunit of protein kinase CK2 is required for mouse embryonic development","volume":"28","author":"Lou","year":"2008","journal-title":"Mol. Cell. Biol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Cenariu, D., Iluta, S., Zimta, A.-A., Petrushev, B., Qian, L., Dirzu, N., Tomuleasa, C., Bumbea, H., and Zaharie, F. (2021). Extramedullary hematopoiesis of the liver and spleen. J. Clin. Med., 10.","DOI":"10.3390\/jcm10245831"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1002\/cyto.a.20674","article-title":"Mouse hematopoietic stem cell identification and analysis","volume":"75","author":"Challen","year":"2009","journal-title":"Cytom. Part A: J. Int. Soc. Adv. Cytom."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.cell.2013.08.062","article-title":"The proliferation-quiescence decision is controlled by a bifurcation in CDK2 activity at mitotic exit","volume":"155","author":"Spencer","year":"2013","journal-title":"Cell"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"D1138","DOI":"10.1093\/nar\/gkad993","article-title":"BloodSpot 3.0: A database of gene and protein expression data in normal and malignant haematopoiesis","volume":"52","author":"Demircan","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"6045","DOI":"10.1038\/onc.2016.86","article-title":"Targeting CK2-driven non-oncogene addiction in B-cell tumors","volume":"35","author":"Mandato","year":"2016","journal-title":"Oncogene"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/hon.2694","article-title":"New responsibilities for aged kinases in B-lymphomas","volume":"38","author":"Piazza","year":"2020","journal-title":"Hematol. Oncol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"6544","DOI":"10.18632\/oncotarget.3446","article-title":"Protein kinase CK2 is widely expressed in follicular, Burkitt and diffuse large B-cell lymphomas and propels malignant B-cell growth","volume":"6","author":"Pizzi","year":"2015","journal-title":"Oncotarget"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.3324\/haematol.2013.096438","article-title":"Adult B-cell acute lymphoblastic leukemia cells display decreased PTEN activity and constitutive hyperactivation of PI3K\/Akt pathway despite high PTEN protein levels","volume":"99","author":"Gomes","year":"2014","journal-title":"Haematologica"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1097\/MOH.0b013e3280de2bf1","article-title":"Molecular insights into stress erythropoiesis","volume":"14","author":"Socolovsky","year":"2007","journal-title":"Curr. Opin. Hematol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1038\/nature15530","article-title":"A perisinusoidal niche for extramedullary haematopoiesis in the spleen","volume":"527","author":"Inra","year":"2015","journal-title":"Nature"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.cell.2008.01.025","article-title":"Hematopoiesis: An evolving paradigm for stem cell biology","volume":"132","author":"Orkin","year":"2008","journal-title":"Cell"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.1242\/dev.134866","article-title":"Human haematopoietic stem cell development: From the embryo to the dish","volume":"144","author":"Ivanovs","year":"2017","journal-title":"Development"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1111\/imcb.12035","article-title":"Splenic hematopoietic stem cells display a pre-activated phenotype","volume":"96","author":"Coppin","year":"2018","journal-title":"Immunol. Cell Biol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1016\/S0301-472X(02)00954-2","article-title":"Side population cells from diverse adult tissues are capable of in vitro hematopoietic differentiation","volume":"30","author":"Asakura","year":"2002","journal-title":"Exp. Hematol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1016\/j.exphem.2007.06.002","article-title":"The combined use of Hoechst efflux ability and aldehyde dehydrogenase activity to identify murine and human hematopoietic stem cells","volume":"35","author":"Pearce","year":"2007","journal-title":"Exp. Hematol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/j.stem.2018.04.015","article-title":"Blood development: Hematopoietic stem cell dependence and independence","volume":"22","author":"Dzierzak","year":"2018","journal-title":"Cell Stem Cell"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/S0952-7915(02)00320-5","article-title":"Ontogeny and genetics of the hemato\/lymphopoietic system","volume":"14","author":"Ling","year":"2002","journal-title":"Curr. Opin. Immunol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/s41375-021-01482-0","article-title":"Hematopoiesis and innate immunity: An inseparable couple for good and bad times, bound together by an hormetic relationship","volume":"36","author":"Ratajczak","year":"2022","journal-title":"Leukemia"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"24018","DOI":"10.1074\/jbc.M212260200","article-title":"CK2 phosphorylation of the armadillo repeat region of \u03b2-catenin potentiates Wnt signaling","volume":"278","author":"Song","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.stem.2012.08.006","article-title":"Less is more: Unveiling the functional core of hematopoietic stem cells through knockout mice","volume":"11","author":"Rossi","year":"2012","journal-title":"Cell Stem Cell"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/14\/13\/963\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:57:29Z","timestamp":1760032649000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/14\/13\/963"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,24]]},"references-count":77,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["cells14130963"],"URL":"https:\/\/doi.org\/10.3390\/cells14130963","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,24]]}}}