{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:27:04Z","timestamp":1760059624121,"version":"build-2065373602"},"reference-count":83,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Gait disturbances are among the most prominent motor symptoms in multiple sclerosis (MS), yet their functional characterization in preclinical models remains limited. In this study, we used high-speed ventral plane videography (DigiGait\u2122) to analyze locomotor behavior during 5 weeks of cuprizone-induced demyelination in 10 male C57BL\/6 mice. Gait analysis revealed significant alterations in stride time (left front paw from 0.303 \u00b1 0.01 s to 0.257 \u00b1 0.007 s; p = 0.003), paw angle (right fore paw from \u221213.78 \u00b1 0.928\u00b0 to 5.456 \u00b1 2.146\u00b0; p = 0.003), and midline distance (right hind paw from 1.889 \u00b1 0.099 cm to 1.216 \u00b1 0.096 cm; p = 0.013), particularly in the hind limbs. These behavioral impairments correlated with histopathological findings of reduced myelination and elevated microglial activation in motor-relevant brain regions, including the corpus callosum, caudate-putamen, and motor cortex. Notably, specific gait parameters showed strong correlations with the degree of demyelination, supporting their relevance as functional biomarkers. Our data demonstrate that high-resolution gait analysis provides a sensitive, non-invasive tool to monitor functional deficits in demyelinating models and may aid in evaluating therapeutic efficacy in future studies.<\/jats:p>","DOI":"10.3390\/cells14130969","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T10:44:41Z","timestamp":1750761881000},"page":"969","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High-Speed-Ventral-Plane Videography Identifies Specific Gait Pattern Changes in Cuprizone-Induced Demyelination in Mice"],"prefix":"10.3390","volume":"14","author":[{"given":"Paula","family":"Giesler","sequence":"first","affiliation":[{"name":"Institute of Anatomy, Rostock University Medical Center, Gertrudenstra\u00dfe 9, 18057 Rostock, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5043-9052","authenticated-orcid":false,"given":"Markus","family":"Kipp","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, Rostock University Medical Center, Gertrudenstra\u00dfe 9, 18057 Rostock, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Hawlitschka","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, Rostock University Medical Center, Gertrudenstra\u00dfe 9, 18057 Rostock, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1212\/WNL.0000000000002469","article-title":"Multiple Sclerosis Prevalence in the United States Commercially Insured Population","volume":"86","author":"Dilokthornsakul","year":"2016","journal-title":"Neurology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/ene.13819","article-title":"Multiple Sclerosis\u2014A Review","volume":"26","author":"Dobson","year":"2019","journal-title":"Eur. 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