{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:50:32Z","timestamp":1772160632866,"version":"3.50.1"},"reference-count":100,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["2023\/06116-2"],"award-info":[{"award-number":["2023\/06116-2"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["2023\/10353-0"],"award-info":[{"award-number":["2023\/10353-0"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["2024\/07829-5"],"award-info":[{"award-number":["2024\/07829-5"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["2024\/17577-3"],"award-info":[{"award-number":["2024\/17577-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Background: Huntington\u2019s Disease (HD) remains without disease-modifying treatments, with existing therapies primarily targeting chorea symptoms and offering limited benefits. This study aims to identify druggable genes and potential biomarkers for HD, focusing on using RNA-Seq analysis to uncover molecular targets and improve clinical trial outcomes. Methods: We reanalyzed transcriptomic data from six independent studies comparing cortex samples of HD patients and healthy controls. The Propensity Score Matching (PSM) algorithm was applied to match cases and controls by age. Differential expression analysis (DEA) coupled with machine learning algorithms were coupled to identify differentially expressed genes (DEGs) and potential biomarkers in HD. Results: Our analysis identified 5834 DEGs, including 394 putative druggable genes involved in processes like neuroinflammation, metal ion dysregulation, and blood\u2013brain barrier dysfunction. These genes\u2019 expression levels correlated with CAG repeat length, disease onset, and progression. We also identified FTH1 as a promising biomarker for HD, with its expression downregulated in the prefrontal cortex and upregulated in peripheral blood in a CAG repeat-dependent manner. Conclusions: This study highlights the potential of FTH1 as both a biomarker and a therapeutic target for HD. Advanced bioinformatics approaches like RNA-Seq and PSM are crucial for uncovering novel targets in HD, paving the way for better therapeutic interventions and improved clinical trial outcomes. Further validation of FTH1\u2032s role is needed to confirm its utility in HD.<\/jats:p>","DOI":"10.3390\/cells14130976","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T07:55:43Z","timestamp":1751442943000},"page":"976","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["From Brain to Blood: Uncovering Potential Therapeutical Targets and Biomarkers for Huntington\u2019s Disease Using an Integrative RNA-Seq Analytical Platform (BDASeq\u00ae)"],"prefix":"10.3390","volume":"14","author":[{"given":"Jo\u00e3o Rafael","family":"Dias Pinto","sequence":"first","affiliation":[{"name":"BioDecision Analytics Ltd., S\u00e3o Paulo 01451-917, SP, Brazil"},{"name":"Post-Graduation Program in Structural and Functional Biology, Paulista School of Medicine, Federal University of S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04023-062, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benedito","family":"Faustinoni Neto","sequence":"additional","affiliation":[{"name":"BioDecision Analytics Ltd., S\u00e3o Paulo 01451-917, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2375-5935","authenticated-orcid":false,"given":"Luciana","family":"Munhoz","sequence":"additional","affiliation":[{"name":"Brazilian Huntington Disease Union House, Scientific Committee, S\u00e3o Paulo 01320-010, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Kerkis","sequence":"additional","affiliation":[{"name":"Genetics Laboratory, Butantan Institute, S\u00e3o Paulo 05503-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8288-2577","authenticated-orcid":false,"given":"Rodrigo Pinheiro","family":"Araldi","sequence":"additional","affiliation":[{"name":"BioDecision Analytics Ltd., S\u00e3o Paulo 01451-917, SP, Brazil"},{"name":"Post-Graduation Program in Structural and Functional Biology, Paulista School of Medicine, Federal University of S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04023-062, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.1002\/mds.29228","article-title":"Prevalence and Incidence of Huntington\u2019s Disease: An Updated Systematic Review and Meta-Analysis","volume":"37","author":"Medina","year":"2022","journal-title":"Mov. 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