{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:33:10Z","timestamp":1760059990226,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Institute of Arthritis and Musculoskeletal and Skin Diseases","award":["R01 AR075413"],"award-info":[{"award-number":["R01 AR075413"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Skeletal muscle regeneration requires a reliable source of myogenic progenitor cells capable of forming new fibers and creating a self-renewing satellite cell pool. Human induced pluripotent stem cell (hiPSC)-derived teratomas have emerged as a novel in vivo platform for generating skeletal myogenic progenitors, although in vivo studies to date have provided only an early single-time-point snapshot. In this study, we isolated a specific population of CD82+ ERBB3+ NGFR+ cells from human iPSC-derived teratomas and verified their long-term in vivo regenerative capacity following transplantation into NSG-mdx4Cv mice. Transplanted cells engrafted, expanded, and generated human Dystrophin+ muscle fibers that increased in size over time and persisted stably long-term. A dynamic population of PAX7+ human satellite cells was established, initially expanding post-transplantation and declining moderately between 4 and 8 months as fibers matured. MyHC isoform analysis revealed a time-based shift from embryonic to neonatal and slow fiber types, indicating a slow progressive maturation of the graft. We further show that these progenitors can be cryopreserved and maintain their engraftment potential. Together, these findings give insight into the evolution of teratoma-derived human myogenic stem cell grafts, and highlight the long-term regenerative potential of teratoma-derived human skeletal myogenic progenitors.<\/jats:p>","DOI":"10.3390\/cells14151150","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T12:56:43Z","timestamp":1753448203000},"page":"1150","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Long-Term Engraftment and Satellite Cell Expansion from Human PSC Teratoma-Derived Myogenic Progenitors"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7243-7127","authenticated-orcid":false,"given":"Zahra","family":"Khosrowpour","sequence":"first","affiliation":[{"name":"Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Greg Marzolf Jr. Muscular Dystrophy Center, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nivedha","family":"Ramaswamy","sequence":"additional","affiliation":[{"name":"Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Greg Marzolf Jr. Muscular Dystrophy Center, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elise N.","family":"Engquist","sequence":"additional","affiliation":[{"name":"Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Greg Marzolf Jr. Muscular Dystrophy Center, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0171-1256","authenticated-orcid":false,"given":"Berkay","family":"Dincer","sequence":"additional","affiliation":[{"name":"Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Greg Marzolf Jr. Muscular Dystrophy Center, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alisha M.","family":"Shah","sequence":"additional","affiliation":[{"name":"Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Greg Marzolf Jr. Muscular Dystrophy Center, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9466-0012","authenticated-orcid":false,"given":"Hossam A. N.","family":"Soliman","sequence":"additional","affiliation":[{"name":"Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6002-4125","authenticated-orcid":false,"given":"Natalya A.","family":"Goloviznina","sequence":"additional","affiliation":[{"name":"Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter I.","family":"Karachunski","sequence":"additional","affiliation":[{"name":"Greg Marzolf Jr. Muscular Dystrophy Center, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Department of Neurology, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5579-7534","authenticated-orcid":false,"given":"Michael","family":"Kyba","sequence":"additional","affiliation":[{"name":"Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Greg Marzolf Jr. Muscular Dystrophy Center, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Seale, P., Ishibashi, J., Scim\u00e8, A., and Rudnicki, M.A. (2004). 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