{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T07:26:53Z","timestamp":1763018813440,"version":"build-2065373602"},"reference-count":74,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T00:00:00Z","timestamp":1755129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["HL137266","HL142864","S10 OD030483","HL153124","18EIA33900041","25TPA1473092","AFM-19613","ANR-16-CE14-0013","\u201cANR-10-IDEX-03-02,\u201d","20POST35211149"],"award-info":[{"award-number":["HL137266","HL142864","S10 OD030483","HL153124","18EIA33900041","25TPA1473092","AFM-19613","ANR-16-CE14-0013","\u201cANR-10-IDEX-03-02,\u201d","20POST35211149"]}]},{"name":"American Heart Association","award":["HL137266","HL142864","S10 OD030483","HL153124","18EIA33900041","25TPA1473092","AFM-19613","ANR-16-CE14-0013","\u201cANR-10-IDEX-03-02,\u201d","20POST35211149"],"award-info":[{"award-number":["HL137266","HL142864","S10 OD030483","HL153124","18EIA33900041","25TPA1473092","AFM-19613","ANR-16-CE14-0013","\u201cANR-10-IDEX-03-02,\u201d","20POST35211149"]}]},{"name":"AFM-Telethon Trampoline","award":["HL137266","HL142864","S10 OD030483","HL153124","18EIA33900041","25TPA1473092","AFM-19613","ANR-16-CE14-0013","\u201cANR-10-IDEX-03-02,\u201d","20POST35211149"],"award-info":[{"award-number":["HL137266","HL142864","S10 OD030483","HL153124","18EIA33900041","25TPA1473092","AFM-19613","ANR-16-CE14-0013","\u201cANR-10-IDEX-03-02,\u201d","20POST35211149"]}]},{"name":"ANR","award":["HL137266","HL142864","S10 OD030483","HL153124","18EIA33900041","25TPA1473092","AFM-19613","ANR-16-CE14-0013","\u201cANR-10-IDEX-03-02,\u201d","20POST35211149"],"award-info":[{"award-number":["HL137266","HL142864","S10 OD030483","HL153124","18EIA33900041","25TPA1473092","AFM-19613","ANR-16-CE14-0013","\u201cANR-10-IDEX-03-02,\u201d","20POST35211149"]}]},{"name":"\u201cInvestments for the Future\u201d Program","award":["HL137266","HL142864","S10 OD030483","HL153124","18EIA33900041","25TPA1473092","AFM-19613","ANR-16-CE14-0013","\u201cANR-10-IDEX-03-02,\u201d","20POST35211149"],"award-info":[{"award-number":["HL137266","HL142864","S10 OD030483","HL153124","18EIA33900041","25TPA1473092","AFM-19613","ANR-16-CE14-0013","\u201cANR-10-IDEX-03-02,\u201d","20POST35211149"]}]},{"name":"American Heart Association\u20132020 AHA Postdoctoral Fellowship","award":["HL137266","HL142864","S10 OD030483","HL153124","18EIA33900041","25TPA1473092","AFM-19613","ANR-16-CE14-0013","\u201cANR-10-IDEX-03-02,\u201d","20POST35211149"],"award-info":[{"award-number":["HL137266","HL142864","S10 OD030483","HL153124","18EIA33900041","25TPA1473092","AFM-19613","ANR-16-CE14-0013","\u201cANR-10-IDEX-03-02,\u201d","20POST35211149"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Mitochondrial fission and fusion appear to be relatively infrequent in cardiac cells compared to other cell types; however, the proteins involved in these events are highly expressed in adult cardiomyocytes (ACM). Therefore, these proteins likely have additional non-canonical roles. We have previously shown that DRP1 not only participates in mitochondrial fission processes but also regulates mitochondrial bioenergetics in cardiac tissue. However, it is still unknown where the DRP1 that does not participate in mitochondrial fission is located and what its role is at those non-fission spots. Therefore, this manuscript will clarify whether oligomeric DRP1 is located at the SR\u2013mitochondria interface, a specific region that harbors the Ca2+ microdomains created by Ca2+ release from the SR through the RyR2. The high Ca2+ microdomains and the subsequent Ca2+ uptake by mitochondria through the mitochondrial Ca2+ uniporter complex (MCUC) are essential to regulate mitochondrial bioenergetics during excitation\u2013contraction (EC) coupling. Herein, we aimed to test the hypothesis that mitochondria-bound DRP1 preferentially accumulates at the mitochondria\u2013SR contacts to deploy its function on regulating mitochondrial bioenergetics and that this strategic position is modulated by calcium in a beat-to-beat manner. In addition, the mechanism responsible for such a biased distribution and its functional implications was investigated. High-resolution imaging approaches, cell fractionation, Western blot, 2D blue native gel electrophoresis, and immunoprecipitations were applied to both electrically paced ACM and Langendorff-perfused beating hearts to elucidate the mechanisms of the strategic DRP1 localization. Our data show that in ACM, mitochondria-bound DRP1 clusters in high molecular weight protein complexes at mitochondria-associated membrane (MAM). This clustering requires DRP1 interaction with \u03b2-ACTIN and is fortified by EC coupling-mediated Ca2+ transients. In ACM, DRP1 is anchored at the mitochondria\u2013SR contacts through interactions with \u03b2-ACTIN and Ca2+ transients, playing a fundamental role in regulating mitochondrial physiology.<\/jats:p>","DOI":"10.3390\/cells14161259","type":"journal-article","created":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T14:51:46Z","timestamp":1755183106000},"page":"1259","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Highly Oligomeric DRP1 Strategic Positioning at Mitochondria\u2013Sarcoplasmic Reticulum Contacts in Adult Murine Heart Through ACTIN Anchoring"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0809-0675","authenticated-orcid":false,"given":"Celia","family":"Fernandez-Sanz","sequence":"first","affiliation":[{"name":"Center for Translational Medicine, Department of Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA"},{"name":"University of Bordeaux, CNRS, IBGC, UMR 5095, F-33000 Bordeaux, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"De la Fuente","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine, Department of Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA"},{"name":"Instituto de Biolog\u00eda y Gen\u00e9tica Molecular (IBGM), Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Fisiolog\u00eda, Facultad de Medicina, Universidad de Valladolid and Consejo Superior de Investigaciones Cient\u00edficas (CSIC), Ramon y Cajal, 7, E-47005 Valladolid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuzana","family":"Nichtova","sequence":"additional","affiliation":[{"name":"MitoCare Center for Mitochondrial Imaging Research and Diagnostics, Department of Pathology, Anatomy & Cell Biol., Thomas Jefferson University, Philadelphia, PA 19107, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilen","family":"Federico","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine, Department of Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0243-2697","authenticated-orcid":false,"given":"Stephane","family":"Duvezin-Caubet","sequence":"additional","affiliation":[{"name":"University of Bordeaux, CNRS, IBGC, UMR 5095, F-33000 Bordeaux, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Lanvermann","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine, Department of Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1328-9726","authenticated-orcid":false,"given":"Hui-Ying","family":"Tsai","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine, Department of Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanguo","family":"Xin","sequence":"additional","affiliation":[{"name":"Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA 98109, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gyorgy","family":"Csordas","sequence":"additional","affiliation":[{"name":"MitoCare Center for Mitochondrial Imaging Research and Diagnostics, Department of Pathology, Anatomy & Cell Biol., Thomas Jefferson University, Philadelphia, PA 19107, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9093-412X","authenticated-orcid":false,"given":"Wang","family":"Wang","sequence":"additional","affiliation":[{"name":"Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA 98109, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5413-611X","authenticated-orcid":false,"given":"Arnaud","family":"Mourier","sequence":"additional","affiliation":[{"name":"University of Bordeaux, CNRS, IBGC, UMR 5095, F-33000 Bordeaux, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3838-3601","authenticated-orcid":false,"given":"Shey-Shing","family":"Sheu","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine, Department of Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA 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