{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:32:39Z","timestamp":1787740359760,"version":"build-2784847793"},"reference-count":34,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010582","name":"National Cancer Institute of the National Institutes of Health","doi-asserted-by":"publisher","award":["R43CA275470"],"award-info":[{"award-number":["R43CA275470"]}],"id":[{"id":"10.13039\/100010582","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Appropriate concentrations of reagents, an absence of significant cell clumps and debris, minimization of artifacts and ensuring satisfactory cell preservation directly affect the quality of data generated and cannot be overestimated. Traditionally, cells in suspension are prepared using a cytospin, which uses centrifugal force to concentrate and deposit cells onto a glass slide. Adherent cells are traditionally grown on coverslips located on the bottom of the wells of cell culture plates, or using special chamber slide systems. In our laboratory, we developed and tested simplified homemade approaches for both techniques, allowing users to perform large volume cell functional tests followed by microscopy evaluation without a need for a cytospin, special chamber slide systems or the use of round cover slips. We present methods and illustrative examples involving the cellular uptake of self-delivering oligonucleotides in murine splenocytes and in two adherent human tumor cell lines.<\/jats:p>","DOI":"10.3390\/cells14161271","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T11:31:37Z","timestamp":1755516697000},"page":"1271","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["New Approaches to Old Techniques in Cell Handling for Microscopy"],"prefix":"10.3390","volume":"14","author":[{"given":"Zhanna","family":"Bartosh","sequence":"first","affiliation":[{"name":"Division of Transplant Immunology, Department of Pathology and Laboratory Medicine, Children\u2019s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA"},{"name":"AUM Biotech, LLC, Philadelphia, PA 19104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veenu","family":"Aishwarya","sequence":"additional","affiliation":[{"name":"AUM Biotech, LLC, Philadelphia, PA 19104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7211-5291","authenticated-orcid":false,"given":"Wayne W.","family":"Hancock","sequence":"additional","affiliation":[{"name":"Division of Transplant Immunology, Department of Pathology and Laboratory Medicine, Children\u2019s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2767-8762","authenticated-orcid":false,"given":"Tatiana","family":"Akimova","sequence":"additional","affiliation":[{"name":"Division of Transplant Immunology, Department of Pathology and Laboratory Medicine, Children\u2019s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA"},{"name":"AUM Biotech, LLC, Philadelphia, PA 19104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.4103\/JOC.JOC_240_16","article-title":"Utility of Cytospin and Cell block Technology in Evaluation of Body Fluids and Urine Samples: A Comparative Study","volume":"35","author":"Qamar","year":"2018","journal-title":"J. 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