{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:22:16Z","timestamp":1772252536602,"version":"3.50.1"},"reference-count":98,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T00:00:00Z","timestamp":1756944000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["AR086382"],"award-info":[{"award-number":["AR086382"]}]},{"name":"NIH","award":["956014"],"award-info":[{"award-number":["956014"]}]},{"name":"NIH","award":["GGP19231"],"award-info":[{"award-number":["GGP19231"]}]},{"name":"MDA","award":["AR086382"],"award-info":[{"award-number":["AR086382"]}]},{"name":"MDA","award":["956014"],"award-info":[{"award-number":["956014"]}]},{"name":"MDA","award":["GGP19231"],"award-info":[{"award-number":["GGP19231"]}]},{"name":"Telethon ONLUS","award":["AR086382"],"award-info":[{"award-number":["AR086382"]}]},{"name":"Telethon ONLUS","award":["956014"],"award-info":[{"award-number":["956014"]}]},{"name":"Telethon ONLUS","award":["GGP19231"],"award-info":[{"award-number":["GGP19231"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Tubular aggregate myopathy (TAM) is an inherited skeletal muscle disease associated with progressive muscle weakness, cramps, and myalgia. Tubular aggregates (TAs) are regular arrays of highly ordered and densely packed straight-tubules observed in muscle biopsies; the extensive presence of TAs represent a key histopathological hallmark of this disease in TAM patients. TAM is caused by gain-of-function mutations in proteins that coordinate store-operated Ca2+ entry (SOCE): STIM1 Ca2+ sensor proteins in the sarcoplasmic reticulum (SR) and Ca2+-permeable ORAI1 channels in the surface membrane. Here, we assessed the therapeutic potential of endurance exercise in the form of voluntary wheel running (VWR) in mitigating TAs and muscle weakness in Orai1G100S\/+ (GS) mice harboring a gain-of-function mutation in the ORAI1 pore. Six months of VWR exercise significantly increased specific force production, upregulated biosynthetic and protein translation pathways, and normalized both mitochondrial protein expression and morphology in the soleus of GS mice. VWR also restored Ca2+ store content, reduced the incidence of TAs, and normalized pathways involving the formation of supramolecular complexes in fast twitch muscles of GS mice. In summary, sustained voluntary endurance exercise improved multiple skeletal muscle phenotypes observed in the GS mouse model of TAM.<\/jats:p>","DOI":"10.3390\/cells14171383","type":"journal-article","created":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T15:31:21Z","timestamp":1756999881000},"page":"1383","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Voluntary Wheel Running Mitigates Disease in an Orai1 Gain-of-Function Mouse Model of Tubular Aggregate Myopathy"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6965-0704","authenticated-orcid":false,"given":"Thomas N.","family":"O\u2019Connor","sequence":"first","affiliation":[{"name":"Genetics and Genomics Graduate Program, Department of Biomedical Genetics, University of Rochester Medical Center, Rochester, NY 14642, USA"},{"name":"Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haley M.","family":"Orciuoli","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA"},{"name":"Department of Biology, Biological Sciences, University of Rochester, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sundeep","family":"Malik","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5927-6995","authenticated-orcid":false,"given":"Alice","family":"Brasile","sequence":"additional","affiliation":[{"name":"Center for Advanced Studies and Technology (CAST), University G. d\u2019Annunzio of Chieti-Pescara, I-66100 Chieti, Italy"},{"name":"Department of Medicine and Aging Sciences (DMSI), University G. d\u2019Annunzio of Chieti-Pescara, I-66100 Chieti, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1197-7813","authenticated-orcid":false,"given":"Laura","family":"Pietrangelo","sequence":"additional","affiliation":[{"name":"Center for Advanced Studies and Technology (CAST), University G. d\u2019Annunzio of Chieti-Pescara, I-66100 Chieti, Italy"},{"name":"Department of Medicine and Aging Sciences (DMSI), University G. d\u2019Annunzio of Chieti-Pescara, I-66100 Chieti, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao","family":"He","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Groom","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Leigh","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Mahamed","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5562-350X","authenticated-orcid":false,"given":"Chen","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feliciano","family":"Protasi","sequence":"additional","affiliation":[{"name":"Center for Advanced Studies and Technology (CAST), University G. d\u2019Annunzio of Chieti-Pescara, I-66100 Chieti, Italy"},{"name":"Department of Medicine and Aging Sciences (DMSI), University G. d\u2019Annunzio of Chieti-Pescara, I-66100 Chieti, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3182-1755","authenticated-orcid":false,"given":"Robert T.","family":"Dirksen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,4]]},"reference":[{"key":"ref_1","first-page":"176","article-title":"Excitation-Contraction Coupling in Muscular Response","volume":"25","author":"Sandow","year":"1952","journal-title":"Yale J. 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