{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T23:51:39Z","timestamp":1785455499273,"version":"3.56.0"},"reference-count":78,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,9,6]],"date-time":"2025-09-06T00:00:00Z","timestamp":1757116800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["21\/05445-7"],"award-info":[{"award-number":["21\/05445-7"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["201281\/2018-0"],"award-info":[{"award-number":["201281\/2018-0"]}]},{"name":"Brazilian National Council for Scientific and Technological Development","award":["21\/05445-7"],"award-info":[{"award-number":["21\/05445-7"]}]},{"name":"Brazilian National Council for Scientific and Technological Development","award":["201281\/2018-0"],"award-info":[{"award-number":["201281\/2018-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Chronic kidney disease (CKD) is a common and serious condition in felines. Accordingly, several cell therapies have been studied over the past decades for effective treatments. This study aimed to develop a new lineage of renal progenitor cells for use in cats with CKD. Metanephric and mesonephric progenitor cells were obtained from mesonephros and metanephros tissues of feline conceptuses at four distinct gestational stages. The cultured cells were characterized by their morphology, tumorigenic potential, immunophenotype determined by flow cytometry, and differentiation potential. We then conducted a pilot study in CKD-affected cats, comparing intraperitoneal injections of cultured metanephric progenitor cells (n = 4) to a placebo solution (n = 3). All four cell types exhibited adhesion and colony formation, but showed no tumorigenic potential. Cells tested positive for renal progenitor markers (CD117, Nephron, and WT1), confirming their identity. Treated cats showed no statistically significant differences (p \u2264 0.05) in any of the data analyzed. However, caregivers reported a voluntary increase in appetite after cell administration. Veterinarians confirmed this information during double-blind evaluations conducted after treatment. Although this data are qualitative, no clinical deterioration was observed in cats. Our results suggest that this new lineage of renal progenitor cells did not induce immediate adverse effects, thus supporting its potential for use in cell-based therapies. However, further studies are needed to evaluate its efficacy in treating renal diseases.<\/jats:p>","DOI":"10.3390\/cells14171395","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T08:06:32Z","timestamp":1757318792000},"page":"1395","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development and Characterization of a Novel Lineage of Renal Progenitor Cells for Potential Use in Feline Chronic Kidney Disease: A Preliminary Study"],"prefix":"10.3390","volume":"14","author":[{"given":"Lara Carolina","family":"Mario","sequence":"first","affiliation":[{"name":"Department of Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, Universidade de S\u00e3o Paulo (FMVZ\/USP), S\u00e3o Paulo 05508-270, SP, Brazil"},{"name":"Center of Excellence in Morphological and Surgical Studies (CEMyQ), Universidad de La Frontera, Temuco 1145, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana de Paula","family":"Nhanharelli","sequence":"additional","affiliation":[{"name":"Department of Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, Universidade de S\u00e3o Paulo (FMVZ\/USP), S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica","family":"Borghesi","sequence":"additional","affiliation":[{"name":"Department of Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, Universidade de S\u00e3o Paulo (FMVZ\/USP), S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaela Rodrigues","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Department of Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, Universidade de S\u00e3o Paulo (FMVZ\/USP), S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8733-4932","authenticated-orcid":false,"given":"Hianka Jasmyne Costa","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Department of Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, Universidade de S\u00e3o Paulo (FMVZ\/USP), S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7848-8558","authenticated-orcid":false,"given":"Thamires Santos","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, Universidade de S\u00e3o Paulo (FMVZ\/USP), S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3686-6757","authenticated-orcid":false,"given":"Mariano del","family":"Sol","sequence":"additional","affiliation":[{"name":"Center of Excellence in Morphological and Surgical Studies (CEMyQ), Universidad de La Frontera, Temuco 1145, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5240-6959","authenticated-orcid":false,"given":"Rodrigo da Silva Nunes","family":"Barreto","sequence":"additional","affiliation":[{"name":"Department of Animal Morphology and Physiology, Faculty of Agricultural and Veterinary Sciences, Universidade Estadual Paulista (UNESP), Jaboticabal 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5035-876X","authenticated-orcid":false,"given":"Sandra Maria","family":"Barbalho","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Pharmacology, School of Medicine, Universidade de Mar\u00edlia (UNIMAR), Mar\u00edlia 17525-902, SP, Brazil"},{"name":"Laboratory for Systematic Investigations of Diseases, Department of Biochemistry and Pharmacology, School of Medicine, Universidade de Mar\u00edlia (UNIMAR), Mar\u00edlia 17525-902, SP, Brazil"},{"name":"Department of Structural and Functional Interactions in Rehabilitation, School of Medicine, Universidade de Mar\u00edlia (UNIMAR), Mar\u00edlia 17525-902, SP, Brazil"},{"name":"Department of Research, Research Coordination Center, Universidade de Mar\u00edlia (UNIMAR) Charitable Hospital, Universidade de Mar\u00edlia (UNIMAR), Mar\u00edlia 17525-902, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4979-115X","authenticated-orcid":false,"given":"Maria Angelica","family":"Miglino","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Interactions in Rehabilitation, School of Medicine, Universidade de Mar\u00edlia (UNIMAR), Mar\u00edlia 17525-902, SP, Brazil"},{"name":"Department of Animal Anatomy, School of Veterinary Medicine, Universidade de Mar\u00edlia (UNIMAR), Mar\u00edlia 17525-902, SP, Brazil"},{"name":"Department of Animal Health, Production and Environment, School of Veterinary Medicine, Universidade de Mar\u00edlia (UNIMAR), Mar\u00edlia 17525-902, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,6]]},"reference":[{"key":"ref_1","unstructured":"Chew, D.J., DiBartola, S.P., and Schenck, P.A. 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