{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:59:40Z","timestamp":1790107180770,"version":"4.0.1"},"reference-count":63,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Heat stroke (HS) is a life-threatening condition that leads to neuronal injury, particularly in the prefrontal cortex, though its mechanisms remain unclear. In this study, we established a rat HS model and observed significant inflammatory responses and neuronal pyroptosis in the prefrontal cortex 6 h post-heat exposure, with the injury severity increasing over time. Mechanistically, HS activated the caspase-1\/GSDMD-dependent pyroptosis pathway through NLRP3 inflammasome activation, resulting in IL-1\u03b2 and IL-18 release. Additionally, HS caused a marked increase in homocysteine (Hcy) levels in both the serum and the prefrontal cortex, accompanied by reduced expression of the Hcy metabolic enzymes MTHFR and CSE, suggesting Hcy metabolism disruption. In vitro, Hcy induced pyroptosis in PC12 cells, elevating IL-1\u03b2, IL-18, and LDH levels. Notably, the NLRP3 inhibitor MCC950 mitigated this effect by reducing IL-18 and LDH release. Reducing Hcy in vivo alleviated neuronal pyroptosis and counteracted the YTHDF2-mediated decrease in NLRP3 mRNA m6A modification. Hcy reduced global m6A modification, YTHDF2 expression, and NLRP3 m6A modification in PC12 cells. This study reveals that the activation of a novel m6A-YTHDF2-NLRP3 pathway by Hcy underlies HS-induced neuronal injury, suggesting potential therapeutic targets for HS intervention.<\/jats:p>","DOI":"10.3390\/cells14181437","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T10:51:33Z","timestamp":1757933493000},"page":"1437","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Homocysteine-Mediated Neuronal Pyroptosis Contributes to Brain Injury in Heatstroke Rats by Activating the m6A-YTHDF2-NLRP3 Pathway"],"prefix":"10.3390","volume":"14","author":[{"given":"Shijia","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China"},{"name":"Beijing Institute of Basic Medical Sciences, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Xie","sequence":"additional","affiliation":[{"name":"Beijing Institute of Basic Medical Sciences, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Basic Medical Sciences, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaowei","family":"Sun","sequence":"additional","affiliation":[{"name":"Beijing Institute of Basic Medical Sciences, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China"},{"name":"Beijing Institute of Basic Medical Sciences, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Basic Medical Sciences, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobing","family":"Chen","sequence":"additional","affiliation":[{"name":"Beijing Institute of Basic Medical Sciences, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingjia","family":"Qian","sequence":"additional","affiliation":[{"name":"Beijing Institute of Basic Medical Sciences, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China"},{"name":"Beijing Institute of Basic Medical Sciences, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1978","DOI":"10.1056\/NEJMra011089","article-title":"Heat stroke","volume":"346","author":"Bouchama","year":"2002","journal-title":"N. Engl. J. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"170869","DOI":"10.1016\/j.scitotenv.2024.170869","article-title":"Heat stroke-induced cerebral cortex nerve injury by mitochondrial dysfunction: A comprehensive multi-omics profiling analysis","volume":"919","author":"Fang","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"269","DOI":"10.2174\/1568008054863754","article-title":"Caspases as drug targets in ischemic organ injury","volume":"5","author":"Faubel","year":"2005","journal-title":"Curr. Drug Targets Immune Endocr. Metabol. Disord."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"8187529","DOI":"10.1155\/2019\/8187529","article-title":"Alpha-Lipoic Acid Protects Cardiomyocytes against Heat Stroke-Induced Apoptosis and Inflammatory Responses Associated with the Induction of Hsp70 and Activation of Autophagy","volume":"2019","author":"Shen","year":"2019","journal-title":"Mediators Inflamm."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"111305","DOI":"10.1016\/j.intimp.2023.111305","article-title":"Extracellular histones exacerbate heat stroke-induced liver injury by triggering hepatocyte pyroptosis and liver injury via the TLR9-NLRP3 pathway","volume":"126","author":"Li","year":"2024","journal-title":"Int. Immunopharmacol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/s41422-019-0164-5","article-title":"The molecular machinery of regulated cell death","volume":"29","author":"Tang","year":"2019","journal-title":"Cell Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Chi, H., Chang, H., and Sang, T. (2018). Neuronal Cell Death Mechanisms in Major Neurodegenerative Diseases. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19103082"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Jakubowski, H. (2017). Homocysteine Editing, Thioester Chemistry, Coenzyme A, and the Origin of Coded Peptide Synthesis. Life, 7.","DOI":"10.3390\/life7010006"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2994","DOI":"10.1016\/j.febslet.2006.04.088","article-title":"Mechanisms of homocysteine neurotoxicity in neurodegenerative diseases with special reference to dementia","volume":"580","author":"Obeid","year":"2006","journal-title":"FEBS Lett."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2239","DOI":"10.1016\/j.bbagen.2015.08.013","article-title":"Protein N-homocysteinylation: From cellular toxicity to neurodegeneration","volume":"1850","author":"Sharma","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_11","first-page":"1638","article-title":"The relationship between serum cystatin C, homocysteine, and febrile seizures in children and subsequent epileptic seizures","volume":"31","author":"Zhang","year":"2023","journal-title":"Chin. J. Eugen. Genet."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1515\/jpem-2020-0096","article-title":"Transcobalamin II deficiency in twins with a novel variant in the TCN2 gene: Case report and review of literature","volume":"33","author":"Kose","year":"2020","journal-title":"J. Pediatr. Endocrinol. Metab."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s10020-023-00656-z","article-title":"Homocysteine promotes atherosclerosis through macrophage pyroptosis via endoplasmic reticulum stress and calcium disorder","volume":"29","author":"Zhang","year":"2023","journal-title":"Mol. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1186\/s13045-021-01129-8","article-title":"N6-methyladenosine methyltransferases: Functions, regulation, and clinical potential","volume":"14","author":"Huang","year":"2021","journal-title":"J. Hematol. Oncol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.molcel.2016.05.041","article-title":"Structural Basis for Cooperative Function of Mettl3 and Mettl14 Methyltransferases","volume":"63","author":"Wang","year":"2016","journal-title":"Mol. Cell"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Tian, M., Mao, L., and Zhang, L. (2022). Crosstalk among N6-methyladenosine modification and RNAs in central nervous system injuries. Front. Cell Neurosci., 16.","DOI":"10.3389\/fncel.2022.1013450"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s13041-020-0554-0","article-title":"Epitranscriptomic profiling of N6-methyladenosine-related RNA methylation in rat cerebral cortex following traumatic brain injury","volume":"13","author":"Yu","year":"2020","journal-title":"Mol. Brain"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1754207800","DOI":"10.1177\/2040622320916024","article-title":"N6-methyladenosine demethylases Alkbh5\/Fto regulate cerebral ischemia-reperfusion injury","volume":"11","author":"Xu","year":"2020","journal-title":"Ther. Adv. Chronic Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1038\/s41419-020-03186-2","article-title":"YTHDC1 mitigates ischemic stroke by promoting Akt phosphorylation through destabilizing PTEN mRNA","volume":"11","author":"Zhang","year":"2020","journal-title":"Cell Death Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e13054","DOI":"10.1111\/obr.13054","article-title":"Epigenetic regulation of white adipose tissue in the onset of obesity and metabolic diseases","volume":"21","author":"Cardona","year":"2020","journal-title":"Obes. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Yang, Z.J., Huang, S.Y., Zhong, K.Y., Huang, W.G., Huang, Z.H., He, T.T., Yang, M.T., Wusiman, M., Zhou, D.D., and Chen, S. (2024). Betaine alleviates cognitive impairment induced by homocysteine through attenuating NLRP3-mediated microglial pyroptosis in an m(6)A-YTHDF2-dependent manner. Redox Biol., 69.","DOI":"10.1016\/j.redox.2024.103026"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1038\/s41467-023-36747-y","article-title":"Inhibition of ALKBH5 attenuates I\/R-induced renal injury in male mice by promoting Ccl28 m6A modification and increasing Treg recruitment","volume":"14","author":"Chen","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"96","DOI":"10.4085\/1062-6050-47.1.96","article-title":"National athletic trainers\u2019 association position statement: Preventing sudden death in sports","volume":"47","author":"Casa","year":"2012","journal-title":"J. Athl. Train."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1177\/0734242X07088433","article-title":"Mitigation of global greenhouse gas emissions from waste: Conclusions and strategies from the Intergovernmental Panel on Climate Change (IPCC) Fourth Assessment Report. Working Group III (Mitigation)","volume":"26","author":"Bogner","year":"2008","journal-title":"Waste Manag. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e8417","DOI":"10.1097\/MD.0000000000008417","article-title":"Outcome and risk factors associated with extent of central nervous system injury due to exertional heat stroke","volume":"96","author":"Yang","year":"2017","journal-title":"Medicine"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"173","DOI":"10.7326\/0003-4819-129-3-199808010-00001","article-title":"Near-fatal heat stroke during the 1995 heat wave in Chicago","volume":"129","author":"Dematte","year":"1998","journal-title":"Ann. Intern. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e135","DOI":"10.1016\/j.jstrokecerebrovasdis.2015.02.001","article-title":"Acute Vertebrobasilar Territory Infarcts due to Heat Stroke","volume":"24","author":"Jain","year":"2015","journal-title":"J. Stroke Cerebrovasc. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1186\/s12974-015-0324-6","article-title":"Heat stress-induced memory impairment is associated with neuroinflammation in mice","volume":"12","author":"Lee","year":"2015","journal-title":"J Neuroinflammation"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"S548","DOI":"10.1002\/mds.22062","article-title":"Functional organization of the basal ganglia: Therapeutic implications for Parkinson\u2019s disease","volume":"23","author":"Obeso","year":"2008","journal-title":"Mov. Disord."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1080\/23328940.2016.1174794","article-title":"Regional brain responses in humans during body heating and cooling","volume":"3","author":"Farrell","year":"2016","journal-title":"Temperature"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.5114\/aoms\/116550","article-title":"Microglia polarization in heat-induced early neural injury","volume":"20","author":"Wang","year":"2024","journal-title":"Arch. Med. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1097\/SHK.0b013e3181e761c1","article-title":"Heat stroke induces autophagy as a protection mechanism against neurodegeneration in the brain","volume":"34","author":"Liu","year":"2010","journal-title":"Shock"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1080\/02656736.2021.1931479","article-title":"Ethyl pyruvate ameliorates heat stroke-induced multiple organ dysfunction and inflammatory responses by induction of stress proteins and activation of autophagy in rats","volume":"38","author":"Hu","year":"2021","journal-title":"Int. J. Hyperthermia"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Chen, D., Geng, Y., Deng, Z., Li, P., Xue, S., Xu, T., and Li, G. (2023). Inhibition of TLR4 Alleviates Heat Stroke-Induced Cardiomyocyte Injury by Down-Regulating Inflammation and Ferroptosis. Molecules, 28.","DOI":"10.3390\/molecules28052297"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1007\/s00134-009-1500-x","article-title":"Early organ dysfunction course, cooling time and outcome in classic heatstroke","volume":"35","author":"Pease","year":"2009","journal-title":"Intensive Care Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1113\/expphysiol.2009.047761","article-title":"Treatment of hyperthermia: Is assessment of cooling efficiency enough?","volume":"94","author":"Buchheit","year":"2009","journal-title":"Exp. Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1038\/s41423-022-00905-x","article-title":"Role of pyroptosis in inflammation and cancer","volume":"19","author":"Wei","year":"2022","journal-title":"Cell Mol. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1016\/j.immuni.2012.08.027","article-title":"NLRP1 inflammasome activation induces pyroptosis of hematopoietic progenitor cells","volume":"37","author":"Masters","year":"2012","journal-title":"Immunity"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1038\/s41392-021-00507-5","article-title":"Pyroptosis: Mechanisms and diseases","volume":"6","author":"Yu","year":"2021","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1126\/science.aau2818","article-title":"Pathogen blockade of TAK1 triggers caspase-8-dependent cleavage of gasdermin D and cell death","volume":"362","author":"Orning","year":"2018","journal-title":"Science"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1038\/nature15541","article-title":"Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling","volume":"526","author":"Kayagaki","year":"2015","journal-title":"Nature"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/nri.2016.58","article-title":"Inflammasomes: Mechanism of assembly, regulation and signalling","volume":"16","author":"Broz","year":"2016","journal-title":"Nat. Rev. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1038\/nature15514","article-title":"Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death","volume":"526","author":"Shi","year":"2015","journal-title":"Nature"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.immuni.2017.11.013","article-title":"The Pore-Forming Protein Gasdermin D Regulates Interleukin-1 Secretion from Living Macrophages","volume":"48","author":"Evavold","year":"2018","journal-title":"Immunity"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1242\/dev.116.1.201","article-title":"NeuN, a neuronal specific nuclear protein in vertebrates","volume":"116","author":"Mullen","year":"1992","journal-title":"Development"},{"key":"ref_46","first-page":"7","article-title":"Hyperhomocysteinemia and cardiovascular diseases","volume":"80","author":"Guieu","year":"2022","journal-title":"Ann. Biol. Clin."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1007\/s12031-013-0122-5","article-title":"Nutri-epigenetics ameliorates blood-brain barrier damage and neurodegeneration in hyperhomocysteinemia: Role of folic acid","volume":"52","author":"Kalani","year":"2014","journal-title":"J. Mol. Neurosci."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Holbrook, J.A., Jarosz-Griffiths, H.H., Caseley, E., Lara-Reyna, S., Poulter, J.A., Williams-Gray, C.H., Peckham, D., and McDermott, M.F. (2021). Neurodegenerative Disease and the NLRP3 Inflammasome. Front. Pharmacol., 12.","DOI":"10.3389\/fphar.2021.643254"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.tibs.2016.10.004","article-title":"Pyroptosis: Gasdermin-Mediated Programmed Necrotic Cell Death","volume":"42","author":"Shi","year":"2017","journal-title":"Trends Biochem. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1007\/s00011-023-01790-4","article-title":"Role of NLRP3 inflammasome-mediated neuronal pyroptosis and neuroinflammation in neurodegenerative diseases","volume":"72","author":"Han","year":"2023","journal-title":"Inflamm. Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1084\/jem.20150237","article-title":"NLR members NLRC4 and NLRP3 mediate sterile inflammasome activation in microglia and astrocytes","volume":"214","author":"Freeman","year":"2017","journal-title":"J. Exp. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1038\/s41589-019-0278-6","article-title":"MCC950 closes the active conformation of NLRP3 to an inactive state","volume":"15","author":"Arostegui","year":"2019","journal-title":"Nat. Chem. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1146\/annurev.immunol.021908.132612","article-title":"Immunological and inflammatory functions of the interleukin-1 family","volume":"27","author":"Dinarello","year":"2009","journal-title":"Annu. Rev. Immunol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1016\/j.cell.2010.01.040","article-title":"The inflammasomes","volume":"140","author":"Schroder","year":"2010","journal-title":"Cell"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1038\/s41392-020-00450-x","article-title":"The role of m6A modification in the biological functions and diseases","volume":"6","author":"Jiang","year":"2021","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1186\/s13046-021-01952-4","article-title":"N6-methyladenosine-dependent signalling in cancer progression and insights into cancer therapies","volume":"40","author":"Tan","year":"2021","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1038\/s41571-023-00774-x","article-title":"The roles and implications of RNA m(6)A modification in cancer","volume":"20","author":"Deng","year":"2023","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"S40","DOI":"10.1007\/PL00014300","article-title":"The metabolism of homocysteine: Pathways and regulation","volume":"157","author":"Finkelstein","year":"1998","journal-title":"Eur. J. Pediatr."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1146\/annurev.nutr.19.1.217","article-title":"Homocysteine metabolism","volume":"19","author":"Selhub","year":"1999","journal-title":"Annu. Rev. Nutr."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2811","DOI":"10.1093\/jn\/131.11.2811","article-title":"Intracellular S-adenosylhomocysteine concentrations predict global DNA hypomethylation in tissues of methyl-deficient cystathionine beta-synthase heterozygous mice","volume":"131","author":"Caudill","year":"2001","journal-title":"J. Nutr."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"10822","DOI":"10.1016\/S0021-9258(19)70381-0","article-title":"S-Adenosylmethionine and S-adenosylhomocystein metabolism in isolated rat liver. Effects of L-methionine, L-homocystein, and adenosine","volume":"255","author":"Hoffman","year":"1980","journal-title":"J. Biol. Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2361S","DOI":"10.1093\/jn\/132.8.2361S","article-title":"Elevation in S-adenosylhomocysteine and DNA hypomethylation: Potential epigenetic mechanism for homocysteine-related pathology","volume":"132","author":"James","year":"2002","journal-title":"J. Nutr."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/nature12730","article-title":"N6-methyladenosine-dependent regulation of messenger RNA stability","volume":"505","author":"Wang","year":"2014","journal-title":"Nature"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/14\/18\/1437\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:45:43Z","timestamp":1760035543000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/14\/18\/1437"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,15]]},"references-count":63,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["cells14181437"],"URL":"https:\/\/doi.org\/10.3390\/cells14181437","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,15]]}}}