{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:37:16Z","timestamp":1787027836660,"version":"build-2736575974"},"reference-count":53,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T00:00:00Z","timestamp":1758499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"King Abdulaziz University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Fibromyalgia syndrome (FMS) is a chronic disorder marked by widespread musculoskeletal pain, fatigue, mood disturbances, and cognitive impairments. Current treatments primarily focus on symptom management. Ashwagandha (Withania somnifera), a traditional Ayurvedic herb, is known for its adaptogenic and neuroprotective properties. This study evaluated the protective effects of the methanolic root extract of Ashwagandha (ARE) in a reserpine-induced fibromyalgia model in male Swiss albino mice. Mice received oral ARE (100 mg\/kg) for 17 days and reserpine (0.5 mg\/kg, subcutaneously) for three consecutive days to induce fibromyalgia-like symptoms. Behavioral assessments included Von Frey, tail suspension, rotarod, and Y-maze tests. Histological analysis was conducted on the hippocampus and thalamus; however, neurochemical analysis focused on markers such as serotonin, norepinephrine, IL-1\u03b2, TNF\u03b1, MDA, and NO. Results indicated that ARE significantly reduced pain and depressive-like behavior and improved motor function (p &lt; 0.0001); however, no significant changes were observed in open-field locomotion. Histological examination revealed protection of Ashwagandha against neurodegeneration and improved hippocampal integrity, accompanied by increased serotonin and norepinephrine levels and decreased pro-inflammatory cytokines. These findings suggest that Ashwagandha root extract may offer therapeutic benefits for managing fibromyalgia symptoms.<\/jats:p>","DOI":"10.3390\/cells14181478","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T11:18:16Z","timestamp":1758539896000},"page":"1478","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ashwagandha Root Extract Mitigates Fibromyalgia-like Symptoms via Neurochemical and Histological Modulation in Mice"],"prefix":"10.3390","volume":"14","author":[{"given":"Razan Fawaz","family":"Hasanyn","sequence":"first","affiliation":[{"name":"Department of Biological Science, Faculty of Science, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia"},{"name":"Neuroscience and Geroscience Research Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashwaq H.","family":"Batawi","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Faculty of Science, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3071-6854","authenticated-orcid":false,"given":"Mona A.","family":"AL-Thepyani","sequence":"additional","affiliation":[{"name":"Neuroscience and Geroscience Research Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia"},{"name":"Department of Chemistry, College of Sciences & Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reham","family":"Tash","sequence":"additional","affiliation":[{"name":"Anatomy and Embryology Departement, Faculty of Medicine, King Abdulaziz University, Rabigh 25724, Saudi Arabia"},{"name":"Anatomy and Embryology Department, Faculty of Medicine, Ain Shams University, Abassia, Cairo 11566, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6580-4443","authenticated-orcid":false,"given":"Asma","family":"Almuhammadi","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Faculty of Science, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0474-5627","authenticated-orcid":false,"given":"Ashwaq Hassan","family":"Alsabban","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Faculty of Science, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia"},{"name":"Unit of Neurological Disorders, Princess Al-Jawhara Center of Excellence in Research of Hereditary Disorders (PACER.HD), Faculty of Medicine, King Abdulaziz University, Rabigh 25724, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Badrah S.","family":"Alghamdi","sequence":"additional","affiliation":[{"name":"Neuroscience and Geroscience Research Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia"},{"name":"Clinical Physiology Department, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,22]]},"reference":[{"key":"ref_1","first-page":"570","article-title":"The diagnosis of fibromyalgia syndrome","volume":"22","author":"Berwick","year":"2022","journal-title":"Clin. Med. J. R. Coll. 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