{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T16:58:23Z","timestamp":1783702703805,"version":"3.55.0"},"reference-count":29,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T00:00:00Z","timestamp":1758931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002674","name":"Russian Academy of Sciences","doi-asserted-by":"crossref","award":["FGFZ-2025-0006 (125021302128-7)"],"award-info":[{"award-number":["FGFZ-2025-0006 (125021302128-7)"]}],"id":[{"id":"10.13039\/501100002674","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002674","name":"Russian Academy of Sciences","doi-asserted-by":"crossref","award":["FURG-2024-0027 (124021600057-0)"],"award-info":[{"award-number":["FURG-2024-0027 (124021600057-0)"]}],"id":[{"id":"10.13039\/501100002674","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Cells"],"abstract":"<jats:p>Background: Tumor-associated microbiota are implicated in colorectal cancer (CRC). Formalin-fixed paraffin-embedded (FFPE) tumor tissue is widely available yet seldom profiled for microbiota. We tested whether quantitative and presence\/absence signals of selected taxa in FFPE tumors associate with clinicopathological features. Methods: DNA from FFPE primary CRCs (n = 52) was assayed by a targeted PCR panel quantifying 30 bacterial taxa, Candida spp., and total bacterial load. Presence\/absence combinations were selected by the Apriori algorithm with Fisher\u2019s exact testing and 10,000-permutation empirical p-values. Quantitative features were modeled by LASSO logistic regression; discrimination of single taxa and combinations was evaluated by ROC\/AUC. Results: In relative-abundance analyses, Fusobacterium nucleatum showed pro-metastatic value (AUC = 0.622). The best absolute-abundance model for metastasis combined F. nucleatum, Faecalibacterium prausnitzii, total bacterial load, and Akkermansia muciniphila (AUC = 0.739). Anti-metastatic directionality in relative-abundance models was driven by Acinetobacter spp.; the two-taxon set Eubacterium rectale + Acinetobacter spp. achieved AUC = 0.747. Conclusions: PCR-based profiling of FFPE CRC tumors is feasible and reveals hypothesis-generating patterns. Signals linking F. nucleatum to metastatic CRC and Acinetobacter spp. to non-metastatic disease merit validation in larger cohorts; tumor-tissue microbiome features may complement clinicopathological assessment.<\/jats:p>","DOI":"10.3390\/cells14191508","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T10:49:34Z","timestamp":1759142974000},"page":"1508","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Tumor Tissue Microbiota in Colorectal Cancer: PCR Profile of FFPE Blocks in Associations with Metastatic Status"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3386-7746","authenticated-orcid":false,"given":"Nikolay K.","family":"Shakhpazyan","sequence":"first","affiliation":[{"name":"Avtsyn Research Institute of Human Morphology, Petrovsky National Research Center of Surgery, Moscow 119435, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2052-914X","authenticated-orcid":false,"given":"Liudmila M.","family":"Mikhaleva","sequence":"additional","affiliation":[{"name":"Avtsyn Research Institute of Human Morphology, Petrovsky National Research Center of Surgery, Moscow 119435, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3800-1506","authenticated-orcid":false,"given":"Nikolay K.","family":"Sadykhov","sequence":"additional","affiliation":[{"name":"Avtsyn Research Institute of Human Morphology, Petrovsky National Research Center of Surgery, Moscow 119435, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1426-968X","authenticated-orcid":false,"given":"Konstantin Y.","family":"Midiber","sequence":"additional","affiliation":[{"name":"Avtsyn Research Institute of Human Morphology, Petrovsky National Research Center of Surgery, Moscow 119435, Russia"},{"name":"Institute of Medicine, Peoples\u2019 Friendship University of Russia Named After Patrice Lumumba, 6 Miklukho-Maklaya St., Moscow 117198, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3580-1492","authenticated-orcid":false,"given":"Anton S.","family":"Buchaka","sequence":"additional","affiliation":[{"name":"Avtsyn Research Institute of Human Morphology, Petrovsky National Research Center of Surgery, Moscow 119435, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5456-8692","authenticated-orcid":false,"given":"Zarina V.","family":"Gioeva","sequence":"additional","affiliation":[{"name":"Avtsyn Research Institute of Human Morphology, Petrovsky National Research Center of Surgery, Moscow 119435, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9463-8229","authenticated-orcid":false,"given":"Alexander I.","family":"Mikhalev","sequence":"additional","affiliation":[{"name":"Department of Hospital Surgery No. 2, Pirogov Russian National Research Medical University, Moscow 117997, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4377-0035","authenticated-orcid":false,"given":"Arkady L.","family":"Bedzhanyan","sequence":"additional","affiliation":[{"name":"Department of Abdominal Surgery and Oncology II (Coloproctology and Uro-Gynecology), Petrovsky National Research Center of Surgery, Moscow 119435, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Roshandel, G., Ghasemi-Kebria, F., and Malekzadeh, R. 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