{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:56:50Z","timestamp":1759334210007,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pilot & Feasibility grant (to M.A.K.) administered by the Indiana University Center for Diabetes and Metabolic Diseases","award":["P30 DK097512"],"award-info":[{"award-number":["P30 DK097512"]}]},{"name":"IBRI"},{"name":"AnalytixIN"},{"DOI":"10.13039\/100000057","name":"Indiana University Precision Health Initiative","doi-asserted-by":"publisher","award":["1R01GM148970","1R21CA264339"],"award-info":[{"award-number":["1R01GM148970","1R21CA264339"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Indiana University Grand Challenges Precision Health Initiative"}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Cells"],"abstract":"<jats:p>Endocrine cells are dedicated to the production and processing of hormones, from peptides to small molecules, to regulate key physiological processes, including glucose homeostasis and metabolism. Because of this relatively high productivity, endocrine cells must handle a variety of stresses from oxidative stress to the unfolded protein response of the endoplasmic reticulum (UPRER). While much is known about the major pathways regulating the UPRER, the roles of endocrine cell type-specific, context-dependent, and time-dependent transcriptional changes are not well explored. To identify unique and shared responses to the UPRER across a subset of endocrine cell types, we tested representative lines for \u03b2-cells (insulin), \u03b1-cells (glucagon), \u03b4-cells (somatostatin), X\/A-cells (ghrelin), L-cells (glucagon-like peptide 1 (GLP1)), and thyrotropes (thyroid hormone and thyroglobulin). We exposed each cell type to the canonical ER stressor thapsigargin for 6 and 24 h, or vehicle for 24 h, and performed mRNA sequencing. Analysis of the data showed all lines responded to thapsigargin. Comparisons of differentially expressed genes between each line revealed both shared and unique transcriptional signatures. These data represent a valuable mineable set of candidate genes that may have cell type-specific functions during the UPRER and have the potential to lead to a new understanding of how different endocrine cells mitigate or succumb to ER stress.<\/jats:p>","DOI":"10.3390\/cells14191529","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T14:16:29Z","timestamp":1759241789000},"page":"1529","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unique and Conserved Endoplasmic Reticulum Stress Responses in Neuroendocrine Cells"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3012-7171","authenticated-orcid":false,"given":"Karina","family":"Rodrigues-dos-Santos","sequence":"first","affiliation":[{"name":"Indiana Biosciences Research Institute, Indianapolis, IN 46202, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9622-0184","authenticated-orcid":false,"given":"Gitanjali","family":"Roy","sequence":"additional","affiliation":[{"name":"Indiana Biosciences Research Institute, Indianapolis, IN 46202, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7194-1321","authenticated-orcid":false,"given":"Anna","family":"Geisinger","sequence":"additional","affiliation":[{"name":"Indiana Biosciences Research Institute, Indianapolis, IN 46202, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahiti","family":"Somalraju","sequence":"additional","affiliation":[{"name":"Indiana Biosciences Research Institute, Indianapolis, IN 46202, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4628-2256","authenticated-orcid":false,"given":"Travis S.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Indiana Biosciences Research Institute, Indianapolis, IN 46202, USA"},{"name":"Department of Biostatistics, Indiana University School of Medicine, Indianapolis, IN 46202, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8349-9470","authenticated-orcid":false,"given":"Michael A.","family":"Kalwat","sequence":"additional","affiliation":[{"name":"Indiana Biosciences Research Institute, Indianapolis, IN 46202, USA"},{"name":"Center for Diabetes and Metabolic Diseases, Indiana University School of Medicine, Indianapolis, IN 46202, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nrm3270","article-title":"The unfolded protein response: Controlling cell fate decisions under ER stress and beyond","volume":"13","author":"Hetz","year":"2012","journal-title":"Nat. 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