{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:29:36Z","timestamp":1777512576338,"version":"3.51.4"},"reference-count":66,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Montpellier Centre of Excellence in Neurodegeneration","award":["COEN-2023"],"award-info":[{"award-number":["COEN-2023"]}]},{"name":"Association France Alzheimer et Maladies Apparent\u00e9es","award":["AAP-Sciences M\u00e9dicales 2021"],"award-info":[{"award-number":["AAP-Sciences M\u00e9dicales 2021"]}]},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"crossref","award":["ANR-23-CE016-0012"],"award-info":[{"award-number":["ANR-23-CE016-0012"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"crossref","award":["ANR-20-CE18-0036"],"award-info":[{"award-number":["ANR-20-CE18-0036"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"crossref","award":["ANR-24-INBS-0005"],"award-info":[{"award-number":["ANR-24-INBS-0005"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Institut National pour la Sant\u00e9 et la Recherche M\u00e9dicale"},{"DOI":"10.13039\/501100008222","name":"University of Montpellier","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008222","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Tau was initially identified as a microtubule-binding protein critical for microtubule stabilization. It is also a pathological hallmark of tauopathies, a group of neurodegenerative diseases that include Alzheimer\u2019s disease. Under pathological conditions, Tau becomes hyperphosphorylated at numerous sites and aggregates into filamentous deposits, contributing to neuronal cell death and disease progression. While significant research has focused on Tau phosphorylation dynamics and their consequences in pathological contexts, comparatively few studies have investigated Tau phosphorylation during physiological processes, despite the potential relevance to the early onset of pathology. Previous findings have suggested similarities between mitotic Tau phosphorylation and hyperphosphorylation observed in tauopathies, particularly at sites such as AT8, PHF1, S214, and S422. In this study, we quantified the relative levels of phosphorylation at 12 Tau phospho-epitopes during interphase and mitosis in vitro to establish a preliminary mitotic phospho-Tau signature, which was subsequently validated in vivo. Our results demonstrated pronounced phosphorylation of Tau at AT8, p-T217, and p-S422 epitopes during mitosis, both in vitro and in vivo. These findings provide new insights into the physiological phosphorylation of Tau and its potential links to pathological processes.<\/jats:p>","DOI":"10.3390\/cells14201638","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T13:42:03Z","timestamp":1761054123000},"page":"1638","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phospho-Tau Signature During Mitosis: AT8, p-T217 and p-S422 as Key Phospho-Epitopes"],"prefix":"10.3390","volume":"14","author":[{"given":"Marion","family":"Goussard","sequence":"first","affiliation":[{"name":"IGF, Univ Montpellier, CNRS, Inserm, 34094 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly","family":"Zarka","sequence":"additional","affiliation":[{"name":"IGF, Univ Montpellier, CNRS, Inserm, 34094 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morgane","family":"Denus","sequence":"additional","affiliation":[{"name":"IGF, Univ Montpellier, CNRS, Inserm, 34094 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Curel","sequence":"additional","affiliation":[{"name":"IGF, Univ Montpellier, CNRS, Inserm, 34094 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0576-5518","authenticated-orcid":false,"given":"Sylvie","family":"Claeysen","sequence":"additional","affiliation":[{"name":"IGF, Univ Montpellier, CNRS, Inserm, 34094 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4953-3185","authenticated-orcid":false,"given":"Bruno","family":"Lefebvre","sequence":"additional","affiliation":[{"name":"Inserm, CHU Lille, CNRS, LilNCog\u2014Lille Neuroscience & Cognition, University of Lille, 59000 Lille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malika","family":"Hamdane","sequence":"additional","affiliation":[{"name":"Inserm, CHU Lille, CNRS, LilNCog\u2014Lille Neuroscience & Cognition, University of Lille, 59000 Lille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5977-7274","authenticated-orcid":false,"given":"Philippe","family":"Marin","sequence":"additional","affiliation":[{"name":"IGF, Univ Montpellier, CNRS, Inserm, 34094 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5430-1680","authenticated-orcid":false,"given":"Julien","family":"Villeneuve","sequence":"additional","affiliation":[{"name":"IGF, Univ Montpellier, CNRS, Inserm, 34094 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6133-3413","authenticated-orcid":false,"given":"Marie-Laure","family":"Parmentier","sequence":"additional","affiliation":[{"name":"IGF, Univ Montpellier, CNRS, Inserm, 34094 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.1073\/pnas.72.5.1858","article-title":"A Protein Factor Essential for Microtubule Assembly","volume":"72","author":"Weingarten","year":"1975","journal-title":"Proc. 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